A developed method was used for the enhancement of arachidonic acid production by M. isabellina. An orthogonal, rotatable and central composite design was applied to determine the optimum conditions for protoplast reg...A developed method was used for the enhancement of arachidonic acid production by M. isabellina. An orthogonal, rotatable and central composite design was applied to determine the optimum conditions for protoplast regeneration mutagenesis. The results showed that a commixture enzyme （cellulase and glusulase） at the concentration of 4%, enzymolysis temperature at 30℃ and enzymolysis time on 7.5 h were the optimal conditions, in which the lethality of M. isabellina spores was 78.4%. After mutagenesis and re-screenings, M. isabellina mutant Y-69 was obtained. GC analysis showed that the yield of arachidonic acid by Y-69 （2.92 g. L-1） was 3.56 times higher than that of the wild-type strain （0.82 g.L-1）. Pass generation tests showed that the properties of Y-69 by mutation were readily inherited.展开更多
目的从课程特点和培养学生兴趣的角度出发,分析微生物学检验实验教学改革的必要性,并初步探讨微生物学检验实验教学模式中使用模块式技能训练(modules of employable skills,MES)模式的效果。方法选取2020年7月在齐齐哈尔医学院附属第...目的从课程特点和培养学生兴趣的角度出发,分析微生物学检验实验教学改革的必要性,并初步探讨微生物学检验实验教学模式中使用模块式技能训练(modules of employable skills,MES)模式的效果。方法选取2020年7月在齐齐哈尔医学院附属第二医院实习的36名医学检验技术专业学生作为试验组,使用检验流程融入MES训练的教学模式;选取36名2019年实习的医学检验技术专业学生作为对照组,采取传统的教学模式。对比两组教学效果。结果和对照组的学生理论考核知识相比较,试验组学生的理论考核知识分数较高,差异有统计学意义(P<0.05);与对照组学生的试验技能测试考核分数相比,试验组学生的分数较高,差异有统计学意义(P<0.05);两组学生对课程的兴趣相比较,试验组学生的兴趣更高,差异有统计学意义(P<0.05)。结论在学生的微生物教学的过程中采用MES模式联合检验教学模式,可以取得较好的效果,具有一定的推广价值。展开更多
基金Supported by Young Scientifi c Fund of Heilongjiang Province (QC2010093)Project of Daqing Scientifi c Program (sggh2009-029)
文摘A developed method was used for the enhancement of arachidonic acid production by M. isabellina. An orthogonal, rotatable and central composite design was applied to determine the optimum conditions for protoplast regeneration mutagenesis. The results showed that a commixture enzyme （cellulase and glusulase） at the concentration of 4%, enzymolysis temperature at 30℃ and enzymolysis time on 7.5 h were the optimal conditions, in which the lethality of M. isabellina spores was 78.4%. After mutagenesis and re-screenings, M. isabellina mutant Y-69 was obtained. GC analysis showed that the yield of arachidonic acid by Y-69 （2.92 g. L-1） was 3.56 times higher than that of the wild-type strain （0.82 g.L-1）. Pass generation tests showed that the properties of Y-69 by mutation were readily inherited.
文摘目的从课程特点和培养学生兴趣的角度出发,分析微生物学检验实验教学改革的必要性,并初步探讨微生物学检验实验教学模式中使用模块式技能训练(modules of employable skills,MES)模式的效果。方法选取2020年7月在齐齐哈尔医学院附属第二医院实习的36名医学检验技术专业学生作为试验组,使用检验流程融入MES训练的教学模式;选取36名2019年实习的医学检验技术专业学生作为对照组,采取传统的教学模式。对比两组教学效果。结果和对照组的学生理论考核知识相比较,试验组学生的理论考核知识分数较高,差异有统计学意义(P<0.05);与对照组学生的试验技能测试考核分数相比,试验组学生的分数较高,差异有统计学意义(P<0.05);两组学生对课程的兴趣相比较,试验组学生的兴趣更高,差异有统计学意义(P<0.05)。结论在学生的微生物教学的过程中采用MES模式联合检验教学模式,可以取得较好的效果,具有一定的推广价值。
