目的:探索中国人群对于健康相关生命质量(Health-related quality of life,HRQoL)定义的理解,构建中国人群HRQoL概念框架,用于指导中国人群HRQoL测量工具研发。方法:从全国31个省市招募中国一般人群与疾病患者样本,通过开展一对一半结...目的:探索中国人群对于健康相关生命质量(Health-related quality of life,HRQoL)定义的理解,构建中国人群HRQoL概念框架,用于指导中国人群HRQoL测量工具研发。方法:从全国31个省市招募中国一般人群与疾病患者样本,通过开展一对一半结构化质性访谈收集其对HRQoL的看法与理解。基于扎根理论对质性信息进行多层次编码,利用框架法对编码进行梳理、归纳,构建中国HRQoL概念框架。结果:共纳入68名受访者,研究从质性信息中提取1558个一级编码,分析整理为180个二级编码、31个三级编码,最终归纳出6个主题的中国人群HRQoL概念框架。结论:基于中国人群健康观念构建的HRQoL概念框架包含症状、身体功能、情绪、认知、活动、社会功能6个主题,其中尤以胃口/食欲、睡眠和疲乏维度显示出HRQoL概念的中国文化特异性。展开更多
Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveo...Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveolar bone formation and mineralization,and periodontal function.Bone resorption is controlled by a complex molecular network,and BSP can promote osteoclast differentiation and bone resorption.It is also associated with the metastasis of a range of malignancies.Osteoclasts(OC)are thought to be the only cells involved in bone resorption and play an important role in bone formation and late developmental remodeling.Osteoporosis and periodontal disease are caused by excessive bone resorption.This article will summarize the osteoclasts differentiation,the biological function of bone resorption,and explore the progress of the prevention and treatment of the related bone resorption diseases such as osteoporosis and periodontal disease through the regulation of osteoclasts.展开更多
目的:述评健康与幸福感量表(EuroQoL Health and Wellbeing,EQ-HWB)健康状态分级系统的构建过程,为国内学者了解国际新量表、积累量表研发经验提供参考。方法:从PubMed、Embase、知网和万方数据库中检索并纳入所有与EQ-HWB量表研发相关...目的:述评健康与幸福感量表(EuroQoL Health and Wellbeing,EQ-HWB)健康状态分级系统的构建过程,为国内学者了解国际新量表、积累量表研发经验提供参考。方法:从PubMed、Embase、知网和万方数据库中检索并纳入所有与EQ-HWB量表研发相关的文献,从中梳理并总结量表健康状态分级系统构建的步骤方法和经验启示。结果:研究共纳入11篇文献,均为英文文献。EQ-HWB量表的健康状态分级系统通过识别量表维度、组成备选条目池、基于表面效度筛选条目、基于心理测量学筛选条目四个步骤完成构建。其研发经验启发未来研发者应基于广泛来源与透明过程开展维度识别,重点关注条目的可读性、一致性和合理性,且在设定量表心理测量学验证的亚组样本和分析指标时多加考虑。结论:通过纳入丰富维度,EQ-HWB量表能够广泛测量健康相关生命质量、幸福感、社会护理相关生命质量和护理者相关生命质量。但其测量性能与应用场景,特别是在中国人群中的表现仍待进一步探索与研究。展开更多
In order to generate transgenic donor cells for nuclear transfer, bovine fetal fibroblasts were isolated in vitro and transfected with the eukaryotic expression vector pSRA-EGFP-Ipr1. The mouse Ipr1 gene and human SR-...In order to generate transgenic donor cells for nuclear transfer, bovine fetal fibroblasts were isolated in vitro and transfected with the eukaryotic expression vector pSRA-EGFP-Ipr1. The mouse Ipr1 gene and human SR-A promoter were successfully cloned and then used to construct this macrophage-specific eukaryotic expression vector. Bovine fetal fibroblasts in stable primary culture (4th passage) were transfected with pSRA-EGFP-Ipr1 by electroporation. Fluorescence from GFP was observed after 24h. Transgenic cells were selected using G418 and the resultant monoclones were picked and expanded. The transgenic cells, at the 9 th passage, were evaluated by PCR and flow cytometry. The inserted Ipr1 was confirmed by PCR, indicating stable integration of the transgene into the genome and cells had normal karyotypes and very good appearance, which indicate no deleterious result of the transgenesis. In conclusion, the cells obtained could be used as donor cells for nuclear transfer for further research of transgenic cattle.展开更多
Objective:To investigate the effects of Danmu Extract Syrup(DMS) on lipopolysaccharide(LPS)-induced acute lung injury(ALI) in mice and explore the mechanism.Methods:Seventy-two male Balb/C mice were randomly divided i...Objective:To investigate the effects of Danmu Extract Syrup(DMS) on lipopolysaccharide(LPS)-induced acute lung injury(ALI) in mice and explore the mechanism.Methods:Seventy-two male Balb/C mice were randomly divided into 6 groups according to a random number table(n=12),including control(normal saline),LPS(5 mg/kg),LPS+DMS 2.5 mL/kg,LPS+DMS 5 mL/kg,LPS+DMS 10 mL/kg,and LPS+Dexamethasone(DXM,5 mg/kg) groups.After pretreatment with DMS and DXM,the ALI mice model was induced by LPS,and the bronchoalveolar lavage fluid(BALF) were collected to determine protein concentration,cell counts and inflammatory cytokines.The lung tissues of mice were stained with hematoxylin-eosin,and the wet/dry weight ratio(W/D) of lung tissue was calculated.The levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-1βin BALF of mice were detected by enzyme linked immunosorbent assay.The expression levels of Claudin-5,vascular endothelial(VE)-cadherin,vascular endothelial growth factor(VEGF),phospho-protein kinase B(p-Akt) and Akt were detected by Western blot analysis.Results:DMS pre-treatment significantly ameliorated lung histopathological changes.Compared with the LPS group,the W/D ratio and protein contents in BALF were obviously reduced after DMS pretreatment(P<0.05 or P<0.01).The number of cells in BALF and myeloperoxidase(MPO) activity decreased significantly after DMS pretreatment(P<0.05 or P<0.01).DMS pre-treatment decreased the levels of TNF-α,IL-6 and IL-1β(P<0.01).Meanwhile,DMS activated the phosphoinositide 3-kinase/protein kinase B(PI3K/Akt) pathway and reversed the expressions of Claudin-5,VE-cadherin and VEGF(P<0.01).Conclusions:DMS attenuated LPS-induced ALI in mice through repairing endothelial barrier.It might be a potential therapeutic drug for LPS-induced lung injury.展开更多
文摘目的:探索中国人群对于健康相关生命质量(Health-related quality of life,HRQoL)定义的理解,构建中国人群HRQoL概念框架,用于指导中国人群HRQoL测量工具研发。方法:从全国31个省市招募中国一般人群与疾病患者样本,通过开展一对一半结构化质性访谈收集其对HRQoL的看法与理解。基于扎根理论对质性信息进行多层次编码,利用框架法对编码进行梳理、归纳,构建中国HRQoL概念框架。结果:共纳入68名受访者,研究从质性信息中提取1558个一级编码,分析整理为180个二级编码、31个三级编码,最终归纳出6个主题的中国人群HRQoL概念框架。结论:基于中国人群健康观念构建的HRQoL概念框架包含症状、身体功能、情绪、认知、活动、社会功能6个主题,其中尤以胃口/食欲、睡眠和疲乏维度显示出HRQoL概念的中国文化特异性。
基金National Natural Science Foundation Project(No.81260275)。
文摘Bone sialoprotein(BSP)is an important non-collagen extracellular matrix protein(EMC)that promotes bone formation and induces bone resorption.BSP is secreted by odontoblasts,it plays an important role in cementum,alveolar bone formation and mineralization,and periodontal function.Bone resorption is controlled by a complex molecular network,and BSP can promote osteoclast differentiation and bone resorption.It is also associated with the metastasis of a range of malignancies.Osteoclasts(OC)are thought to be the only cells involved in bone resorption and play an important role in bone formation and late developmental remodeling.Osteoporosis and periodontal disease are caused by excessive bone resorption.This article will summarize the osteoclasts differentiation,the biological function of bone resorption,and explore the progress of the prevention and treatment of the related bone resorption diseases such as osteoporosis and periodontal disease through the regulation of osteoclasts.
文摘目的:述评健康与幸福感量表(EuroQoL Health and Wellbeing,EQ-HWB)健康状态分级系统的构建过程,为国内学者了解国际新量表、积累量表研发经验提供参考。方法:从PubMed、Embase、知网和万方数据库中检索并纳入所有与EQ-HWB量表研发相关的文献,从中梳理并总结量表健康状态分级系统构建的步骤方法和经验启示。结果:研究共纳入11篇文献,均为英文文献。EQ-HWB量表的健康状态分级系统通过识别量表维度、组成备选条目池、基于表面效度筛选条目、基于心理测量学筛选条目四个步骤完成构建。其研发经验启发未来研发者应基于广泛来源与透明过程开展维度识别,重点关注条目的可读性、一致性和合理性,且在设定量表心理测量学验证的亚组样本和分析指标时多加考虑。结论:通过纳入丰富维度,EQ-HWB量表能够广泛测量健康相关生命质量、幸福感、社会护理相关生命质量和护理者相关生命质量。但其测量性能与应用场景,特别是在中国人群中的表现仍待进一步探索与研究。
基金supported by A key special project of breeding for disease resistance of PR china(Project No.2008ZX08007-004)
文摘In order to generate transgenic donor cells for nuclear transfer, bovine fetal fibroblasts were isolated in vitro and transfected with the eukaryotic expression vector pSRA-EGFP-Ipr1. The mouse Ipr1 gene and human SR-A promoter were successfully cloned and then used to construct this macrophage-specific eukaryotic expression vector. Bovine fetal fibroblasts in stable primary culture (4th passage) were transfected with pSRA-EGFP-Ipr1 by electroporation. Fluorescence from GFP was observed after 24h. Transgenic cells were selected using G418 and the resultant monoclones were picked and expanded. The transgenic cells, at the 9 th passage, were evaluated by PCR and flow cytometry. The inserted Ipr1 was confirmed by PCR, indicating stable integration of the transgene into the genome and cells had normal karyotypes and very good appearance, which indicate no deleterious result of the transgenesis. In conclusion, the cells obtained could be used as donor cells for nuclear transfer for further research of transgenic cattle.
基金Supported by the National Natural Science Foundation of China (No.82160826)Hainan Provincial Natural Science Foundation of China (No.2019RC207)。
文摘Objective:To investigate the effects of Danmu Extract Syrup(DMS) on lipopolysaccharide(LPS)-induced acute lung injury(ALI) in mice and explore the mechanism.Methods:Seventy-two male Balb/C mice were randomly divided into 6 groups according to a random number table(n=12),including control(normal saline),LPS(5 mg/kg),LPS+DMS 2.5 mL/kg,LPS+DMS 5 mL/kg,LPS+DMS 10 mL/kg,and LPS+Dexamethasone(DXM,5 mg/kg) groups.After pretreatment with DMS and DXM,the ALI mice model was induced by LPS,and the bronchoalveolar lavage fluid(BALF) were collected to determine protein concentration,cell counts and inflammatory cytokines.The lung tissues of mice were stained with hematoxylin-eosin,and the wet/dry weight ratio(W/D) of lung tissue was calculated.The levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-1βin BALF of mice were detected by enzyme linked immunosorbent assay.The expression levels of Claudin-5,vascular endothelial(VE)-cadherin,vascular endothelial growth factor(VEGF),phospho-protein kinase B(p-Akt) and Akt were detected by Western blot analysis.Results:DMS pre-treatment significantly ameliorated lung histopathological changes.Compared with the LPS group,the W/D ratio and protein contents in BALF were obviously reduced after DMS pretreatment(P<0.05 or P<0.01).The number of cells in BALF and myeloperoxidase(MPO) activity decreased significantly after DMS pretreatment(P<0.05 or P<0.01).DMS pre-treatment decreased the levels of TNF-α,IL-6 and IL-1β(P<0.01).Meanwhile,DMS activated the phosphoinositide 3-kinase/protein kinase B(PI3K/Akt) pathway and reversed the expressions of Claudin-5,VE-cadherin and VEGF(P<0.01).Conclusions:DMS attenuated LPS-induced ALI in mice through repairing endothelial barrier.It might be a potential therapeutic drug for LPS-induced lung injury.
