When evaluating an area's seismic risk or resilience,it is necessary to use the spatial correlation to analyze the ground motion parameters of multiple sites together in an earthquake.These two large earthquakes i...When evaluating an area's seismic risk or resilience,it is necessary to use the spatial correlation to analyze the ground motion parameters of multiple sites together in an earthquake.These two large earthquakes in Türkiye provided the possibility for spatial correlation analysis of ground motion intensity measurements in this area.Based on the strong motion records provided by The Disaster and Emergency Management Authority of Türkiye(AFAD),this study uses the local ground motion prediction equation in Türkiye to give spatial correlation analysis of Intensity Measurements.This study gives an exponential model based on a semivariogram and compares it with the correlation model obtained from previous studies.展开更多
Objective:Pancreatic ductal adenocarcinoma(PDAC)is a highly malignant gastrointestinal cancer with a 5-year survival rate of only 9%.Of PDAC patients,15%-20%are eligible for radical surgery.Gemcitabine is an important...Objective:Pancreatic ductal adenocarcinoma(PDAC)is a highly malignant gastrointestinal cancer with a 5-year survival rate of only 9%.Of PDAC patients,15%-20%are eligible for radical surgery.Gemcitabine is an important chemotherapeutic agent for patients with PDAC;however,the efficacy of gemcitabine is limited due to resistance.Therefore,reducing gemcitabine resistance is essential for improving survival of patients with PDAC.Identifying the key target that determines gemcitabine resistance in PDAC and reversing gemcitabine resistance using target inhibitors in combination with gemcitabine are crucial steps in the quest to improve survival prognosis in patients with PDAC.Methods:We constructed a human genome-wide CRISPRa/dCas 9 overexpression library in PDAC cell lines to screen key targets of drug resistance based on sgRNA abundance and enrichment.Then,co-IP,ChIP,ChIP-seq,transcriptome sequencing,and qPCR were used to determine the specific mechanism by which phospholipase D1(PLD1)confers resistance to gemcitabine.Results:PLD1 combines with nucleophosmin 1(NPM1)and triggers NPM1 nuclear translocation,where NPM1 acts as a transcription factor to upregulate interleukin 7 receptor(IL7R)expression.Upon interleukin 7(IL-7)binding,IL7R activates the JAK1/STAT5 signaling pathway to increase the expression of the anti-apoptotic protein,BCL-2,and induce gemcitabine resistance.The PLD1 inhibitor,Vu0155069,targets PLD1 to induce apoptosis in gemcitabine-resistant PDAC cells.Conclusions:PLD1 is an enzyme that has a critical role in PDAC-associated gemcitabine resistance through a non-enzymatic interaction with NPM1,further promoting the downstream JAK1/STAT5/Bcl-2 pathway.Inhibiting any of the participants of this pathway can increase gemcitabine sensitivity.展开更多
Fhb7 is a major gene that was transferred from Thinopyrum ponticum to chromosome 7D of wheat(Triticum aestivum)and confers resistance to both Fusarium head blight(FHB)and Fusarium crown rot(FCR).However,Fhb7 is tightl...Fhb7 is a major gene that was transferred from Thinopyrum ponticum to chromosome 7D of wheat(Triticum aestivum)and confers resistance to both Fusarium head blight(FHB)and Fusarium crown rot(FCR).However,Fhb7 is tightly linked to the PSY-E2 gene,which causes yellow flour,limiting its application in breeding.To break this linkage,marker K-PSY was developed for tagging PSY-E2 and used with Fhb7 markers to identify recombination between the two genes.Screening 21,000 BC1F2 backcross progeny(Chinese Spring ph1bph1b*2/SDAU 2028)revealed two Fhb7^(+)wheat-Tp7el_(2)L lines,Shannong 2–16and Shannong 16–1,that carry a desired truncated Fhb7^(+)translocation segment without PSY-E2.The two lines show levels of resistance to FHB and FCR similar to those of the original translocation line SDAU 2028,but have white flour.To facilitate Fhb7 use in wheat breeding,STS markers were developed and used to isolate Fhb7 on a truncated Tp7el_(2) translocation segment.Near-isogenic lines carrying the Fhb7^(+)segment were generated in the backgrounds of three commercial cultivars,and Fhb7^(+)lines showed increased FHB and FCR resistance without yield penalty.The breakage of the tight linkage between Fhb7 and PSY-E2 via homoeologous recombination provides genetic resources for improvement of wheat resistance to FHB and FCR and permit the large-scale deployment of Fhb7 in breeding using marker-assisted selection.展开更多
In order to comprehend the dynamic disaster mechanism induced by overburden rock caving during the advancement of a coal mining face, a physical simulation model is constructed basing on the geological condition of th...In order to comprehend the dynamic disaster mechanism induced by overburden rock caving during the advancement of a coal mining face, a physical simulation model is constructed basing on the geological condition of the 21221 mining face at Qianqiu coal mine in Henan Province, China. This study established, a comprehensive monitoring system to investigate the interrelations and evolutionary characteristics among multiple mechanical parameters, including mining-induced stress, displacement, temperature, and acoustic emission events during overburden rock caving. It is suggested that, despite the uniformity of the overburden rock caving interval, the main characteristic of overburden rock lies in its uneven caving strength. The mining-induced stress exhibits a reasonable interrelation with the displacement, temperature, and acoustic emission events of the rock strata. With the advancement of the coal seam, the mining-induced stress undergoes four successive stages: gentle stability, gradual accumulation, high-level mutation, and a return to stability. The variations in other mechanical parameters does not synchronize with the signifcant changes in mining-induced stress. Before the collapse of overburden rock occurs, rock strata temperature increment decreases and the acoustic emission ringing counts surges with the increase of rock strata displacement and mining-induced stress. Therefore, the collaborative characteristics of mining-induced stress, displacement, temperature, and acoustic emission ringing counts can be identifed as the precursor information or overburden rock caving. These results are in good consistent with on-site situation in the coal mine.展开更多
Reliance on agriculture for food security is a constant in all modern societies.Global climate change and population growth have put immense pressure on sustainable agriculture,exacerbating the effects of environmenta...Reliance on agriculture for food security is a constant in all modern societies.Global climate change and population growth have put immense pressure on sustainable agriculture,exacerbating the effects of environmental stresses.Drought is one of the most pressing abiotic stresses that farmers face,presenting an annual threat to crop growth and yield.Crops have evolved extensive morphological,physiological,and molecular mechanisms to combat drought stress.Drought resistance is a polygenic trait,controlled by a complex genetic network and an array of genes working together to ensure plant survival.Many studies have aimed at dissecting the genetic mechanisms underlying drought resistance.Recent studies using linkage and association mapping have made progress in identifying genetic variations that affect drought-resistance traits.These loci may potentially be engineered by genetic transformation and genome editing aimed at developing new,stress-resistant crop cultivars.Here we summarize recent progress in elucidating the genetic basis of crop drought resistance.Molecular-breeding technologies such as marker-assisted selection,genome selection,gene transformation,and genome editing are currently employed to develop drought-resistant germplasm in a variety of crops.Recent advances in basic research and crop biotechnology covered in this review will facilitate delivery of drought-resistant crops with unprecedented efficiency.展开更多
High-speed rail(HSR) has formed a networked operational scale in China. Any internal or external disturbance may deviate trains’ operation from the planned schedules, resulting in primary delays or even cascading del...High-speed rail(HSR) has formed a networked operational scale in China. Any internal or external disturbance may deviate trains’ operation from the planned schedules, resulting in primary delays or even cascading delays on a network scale. Studying the delay propagation mechanism could help to improve the timetable resilience in the planning stage and realize cooperative rescheduling for dispatchers. To quickly and effectively predict the spatial-temporal range of cascading delays, this paper proposes a max-plus algebra based delay propagation model considering trains’ operation strategy and the systems’ constraints. A double-layer network based breadth-first search algorithm based on the constraint network and the timetable network is further proposed to solve the delay propagation process for different kinds of emergencies. The proposed model could deal with the delay propagation problem when emergencies occur in sections or stations and is suitable for static emergencies and dynamic emergencies. Case studies show that the proposed algorithm can significantly improve the computational efficiency of the large-scale HSR network. Moreover, the real operational data of China HSR is adopted to verify the proposed model, and the results show that the cascading delays can be timely and accurately inferred, and the delay propagation characteristics under three kinds of emergencies are unfolded.展开更多
Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury(CIRI).The exact mechanisms underlying I/R injury are unclear.In this study,we aime...Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury(CIRI).The exact mechanisms underlying I/R injury are unclear.In this study,we aimed to determine the role of m6A-modified methylase complex methyltransferase-like 3(METTL3)in cerebral ischemiareperfusion(I/R)injury.We found that m6A and METTL3 levels increased in OGD/RX-induced mouse astrocytescerebellar(MA-C)and the brain of middle cerebral artery occlusion(MCAO)model mice.METTL3 siRNA treatment reduced OGD-RX-induced MAC cell viability and proliferation,which increased with METTL3 over-expression.Flow cytometry analysis showed that silencing METTL3 significantly enhanced OGD/RX-induced MAC apoptosis,which was significantly reduced with METTL3 up-regulation.In an MCAO model,METTL3 overexpression significantly reduced cerebral infarction area and decreased brain cell apoptosis,indicating that METTL3 OE treatment could ameliorate brain edema and injury.Thus,METTL3 could be used as a target to treat I/R injury.展开更多
With the rapid development of railway transportation,higher requirements for capacity are increasing and amount of communication,computer and control technologies are applied in train control systems which is the popu...With the rapid development of railway transportation,higher requirements for capacity are increasing and amount of communication,computer and control technologies are applied in train control systems which is the popular method for train control.The application of 3 C brings the enhancement of railway transportation performance.However,the number of internal and external cyber security threats is rising,which could lead to some railway accidents.On the other hand,safety is the core of the design process for railway but security is rarely considered due to the closure of traditional railway systems.As cyber security is threatening the normal operation of industrial control systems and the critical infrastructure,security ssues of train control systems should be paid more attention as railway transportation play an important role for society and economy.However,due to the safety-critical characteristics,safety assessment and analysis works have been performed for a long time,but cyber security related works are rarely considered.In the paper,we demonstrate the security situation of train control systems based on the inherent features and the experience of other industrial control systems,where the technical defects and potential threats are summarized.According to the practical engineering experience,the current security protection strategies are illustrated,which shows the popular security protection methods are limited and cannot realize the defense-in-depth.Finally,some research challenges of security issues of train control systems are identified.展开更多
This paper presents an investigation on the characteristics of overlying strata collapse and mininginduced pressure in fault-influenced zone by employing the physical modeling in consideration of fault structure.The p...This paper presents an investigation on the characteristics of overlying strata collapse and mininginduced pressure in fault-influenced zone by employing the physical modeling in consideration of fault structure.The precursory information of fault slip during the underground mining activities is studied as well.Based on the physical modeling,the optimization of roadway support design and the field verification in fault-influenced zone are conducted.Physical modeling results show that,due to the combined effect of mining activities and fault slip,the mining-induced pressure and the extent of damaged rock masses in the fault-influenced zone are greater than those in the uninfluenced zone.The sharp increase and the succeeding stabilization of stress or steady increase in displacement can be identified as the precursory information of fault slip.Considering the larger mining-induced pressure in the fault-influenced zone,the new support design utilizing cables is proposed.The optimization of roadway support design suggests that the cables can be anchored in the stable surrounding rocks and can effectively mobilize the load bearing capacity of the stable surrounding rocks.The field observation indicates that the roadway is in good condition with the optimized roadway support design.展开更多
To enhance the capacity of the radar-reconnaissance interception receiver recognizing linear frequency modulated(LFM) at a low signal-noise ratio, this paper presents WignerHough transform(WHT) of the LFM signal and i...To enhance the capacity of the radar-reconnaissance interception receiver recognizing linear frequency modulated(LFM) at a low signal-noise ratio, this paper presents WignerHough transform(WHT) of the LFM signal and its corresponding characteristics, derives the probability density functions of the LFM signal and Gaussian white noise within WHT based on entropy(WHTE), dimension under different assumptions and puts forward a WHT algorithm based on entropy of slice to improve the capacity of detecting the LFM signal. Entropy of the WHT domain slice is adopted to assess the information size of polar radius or angle slice, which is converted into the weight factor to weight every slice. Double-deck weight is used to weaken the influences of noise and disturbance terms and WHTE treatment and signal detection procedure are also summarized. The rationality of the algorithm is demonstrated through theoretical analysis and formula derivation, the efficiency of the algorithm is verified by simulation comparison between WHT, fractional Fourier transform and periodic WHT, and it is highlighted that the WHTE algorithm has better detection accuracy and range of application against strong noise background.展开更多
Coal bump refers to a sudden catastrophic failure of coal seam and usually can cause serious damages to underground mining facilities and staff. In this circumstance, this paper focuses on the recent achievements in t...Coal bump refers to a sudden catastrophic failure of coal seam and usually can cause serious damages to underground mining facilities and staff. In this circumstance, this paper focuses on the recent achievements in the mechanism and prevention techniques of coal bumps over the past five years in China.Based on theoretical analysis, laboratory experiment, numerical simulation and field test, the characteristics of coal bumps occurrence in China's coal mines were described, and the difference between coal bumps and rockbursts was also discussed. In addition, three categories of coal bumps induced by"material failure" were introduced, i.e. hard roof, floor strata and tectonic structures, in which the mechanism of coal bumps induced by geological structures was analyzed. This involves the bump liability and microstructure effects on bump-prone coal failure, the mechanism of coal bumps in response to fault reactivation, island face mining or hard roof failure. Next, the achievements in the monitoring and controlling methods of coal bumps were reviewed. These methods involve the incorporated prediction system of micro-seismicity and mining-induced pressure, the distributed micro-seismic monitoring system, energy absorption support system, bolts with constant resistance and large elongation,and the "multi-stage" high-performance support. Finally, an optimal mining design is desirable for the purpose of coal bump mitigation.展开更多
This research was undertaken to clarify the characteristics of vegetation change and its main influencing factors on the Qinghai-Tibet Plateau. Using the greenness rate of change(GRC) and correlation factors, we analy...This research was undertaken to clarify the characteristics of vegetation change and its main influencing factors on the Qinghai-Tibet Plateau. Using the greenness rate of change(GRC) and correlation factors, we analyzed the trend of vegetation change and its dominant factors from 2000 to 2015. The results indicate that the vegetation tended to improve from 2000 to 2015 on the Qinghai-Tibet Plateau, with the improved area accounting for 39.93% of the total; and the degraded area accounting for 19.32%. The areas of degraded vegetation are mainly concentrated in the low-relief and intermediate-relief mountains of the high-altitude and extremely high-altitude areas on the Qinghai-Tibet Plateau, as the vegetation characteristics are impacted by the terrain. Temperature and precipitation have obvious response mechanisms to vegetation growth, but the effects of precipitation and temperature on vegetation degradation are not significant over a short time frame. Overgrazing and population growth are the dominant factors of vegetation degradation on the Qinghai-Tibet Plateau.展开更多
The Ludian County of Yunnan Province in southwestern China was struck by an MS6.5 earthquake on August 3, 2014, which was another destructive event following the MS8.0 Wenchuan earthquake in 2008, MS7.1 Yushu earthqua...The Ludian County of Yunnan Province in southwestern China was struck by an MS6.5 earthquake on August 3, 2014, which was another destructive event following the MS8.0 Wenchuan earthquake in 2008, MS7.1 Yushu earthquake in 2010, and MS7.0 Lushan earthquake in 2013. National Strong-Motion Observation Network System of China collected 74 strong motion recordings,which the maximum peak ground acceleration recorded by the 053 LLT station in Longtoushan Town was 949 cm/s2 in E–W component. The observed PGAs and spectral ordinates were compared with ground-motion prediction equation in China and the NGA-West2 developed by Pacific Earthquake Engineering Researcher Center. This earthquake is considered as the first case for testing applicability of NGA-West2 in China. Results indicate that the observed PGAs and the 5 % damped pseudo-response spectral accelerations are significantly lower than the predicted ones. The field survey around some typical strong motion stations verified that the earthquake damage was consistent with the official isoseismal by China Earthquake Administration.展开更多
In this paper, diamond-like carbon (DLC) fi[ms were deposited on Ti alloy by electro-deposition. DLC films were brown and composed of the compact grains whose diameter was about 400 nm. Examined by XPS, the main compo...In this paper, diamond-like carbon (DLC) fi[ms were deposited on Ti alloy by electro-deposition. DLC films were brown and composed of the compact grains whose diameter was about 400 nm. Examined by XPS, the main composition of the films was carbon. In the Raman spectrum, there were a broad peak at 1350 cm-1 and a broad peak at 1600 cm-1, which indicated that the films were DLC films.展开更多
A new Gaussian approximation nonlinear filter called generalized cubature quadrature Kalman filter(GCQKF) is introduced for nonlinear dynamic systems. Based on standard GCQKF,two extensions are developed, namely squar...A new Gaussian approximation nonlinear filter called generalized cubature quadrature Kalman filter(GCQKF) is introduced for nonlinear dynamic systems. Based on standard GCQKF,two extensions are developed, namely square root generalized cubature quadrature Kalman filter(SR-GCQKF) and iterated generalized cubature quadrature Kalman filter(I-GCQKF). In SR-GCQKF,the QR decomposition is exploited to alter the Cholesky decomposition and both predicted and filtered error covariances have been propagated in square root format to make sure the numerical stability. In I-GCQKF, the measurement update step is executed iteratively to make full use of the latest measurement and a new terminal criterion is adopted to guarantee the increase of likelihood. Detailed numerical experiments demonstrate the superior performance on both tracking stability and estimation accuracy of I-GCQKF and SR-GCQKF compared with GCQKF.展开更多
It is generally recognized that Caucasians and Asians have different skin aging features.The aim of this study was to develop a facial wrinkle grading scale for Chinese women.Standard photographs were taken of 242 Chi...It is generally recognized that Caucasians and Asians have different skin aging features.The aim of this study was to develop a facial wrinkle grading scale for Chinese women.Standard photographs were taken of 242 Chinese women.Six sets of 0 to 9 wrinkle scales with reference photographs and descriptions were selected,including grading scales for resting and hyperkinetic crow's feet,frontalis lines,glabellar frown lines,and nasolabial folds.To identify the scale by objective quantitative measurement,skin surface measurements from the Visioscan~ VC98 were used.To test the reliability and validity of our wrinkle scale,a multi-rater consensus method was used.A double-blind,randomized,vehicle-controlled 12-week study was conducted to use this clinical photo-score to evaluate the efficacy and safety of Centella triterpenes cream~ in treating crow's feet.A newly developed 10-point photographic and descriptive scale emerged from this study.The final atlas of these photographs contained a total of 6 sets with 10 pictures each.From 0 to 9,surface evaluation of smoothness(SEsm) parametric measurements decreased progressively,indicating that the scale increased inversely.Weighted kappa coefficients for intra-assessor were between 0.75-0.87.The overall Kendall's coefficient is 0.86 on the first rating and 0.87 on the second rating.Thirtysix volunteers were recruited and 35 subjects completed a 12-week trial.Clinical photo-score by investigator showed a significant difference(P < 0.05) between the treatment side and control side after 4 weeks.Use of these scales in clinical settings to evaluate facial wrinkles in Asians individuals is recommended.展开更多
In response to the development of deep-sea oil and gas resources,which require a high degree of cooperation by crude oil transportation equipment,a new type of ship known as the cargo transfer vessel(CTV)has been deve...In response to the development of deep-sea oil and gas resources,which require a high degree of cooperation by crude oil transportation equipment,a new type of ship known as the cargo transfer vessel(CTV)has been developed.To provide a theoretical reference for the design and equipment of the CTV’s dynamic positioning system,in this paper,we take the new deepwater CTVas the study object and theoretically and numerically analyze its operation,wind load,current load,wave load,and navigational resistance in a range of Brazilian sea conditions with respect to its positioning and towing modes.We confirm that our proposed method can successfully calculate the total environmental load of the CTVand that the CTV is able to operate normally under the designed sea conditions.展开更多
It is well known that the complicated channeling of fluid flow and heat transfer is strongly related with the intricate natural fracture system.However,it is still challenging to set up the fracture network model whic...It is well known that the complicated channeling of fluid flow and heat transfer is strongly related with the intricate natural fracture system.However,it is still challenging to set up the fracture network model which is strong heterogeneous.Compared with other methods(e.g.equivalent continuum model(ECM),discrete fracture model(DFM),and ECM-DFM),the fracture flow module in the COMSOL Multiphysics simulator is powerful in definition of fractures as the inner flow boundary existing in the porous media.Thus it is selected to simulate the fluid flow and heat transfer in the geothermal-developed fractured granite of Sanguliu area located at Liaodong Peninsula,Eastern China.The natural faults/fractures based on field investigation combined with the discrete fracture network(DFN)generated by the MATLAB are used to represent the two-dimensional geological model.Numerical results show that early thermal breakthrough occurs at the production well caused by quick flow of cold water along the highly connected fractures.Suitable hydraulic fracturing treatments with proper injection rates,locations,etc.can efficiently hinder the thermal breakthrough time in the natural fracture system.Large well spacing helps the long-term operation of geothermal production,but it is highly dependent on the geometrical morphology of the fracture network.The enhancement of reservoir properties at the near-well regions can also increase the geothermal production efficiency.The results in this study can provide references to achieve a sustainable geothermal exploitation in fractured granitic geothermal reservoirs or hot dry rocks at depth.展开更多
Visualization of complex geological structures can technically support the accurate prediction and prevention of coal mine disasters.This study proposed a new digital reconstruction method to visualize geological stru...Visualization of complex geological structures can technically support the accurate prediction and prevention of coal mine disasters.This study proposed a new digital reconstruction method to visualize geological structures based on establishing a virtual model in the digital twin system.This methodology for the digital reconstruction of complex fault structures comprises the following four aspects:(1)collection and fdelity of multi-physical feld data of the fault structures,(2)the transmission of multi-physical feld data,(3)the normalization of multi-physical feld data,and(4)digital model reconstruction of fault structures.The key scientifc issues of this methodology to be resolved include in situ fdelity of multi-feld data and normalized programming of multi-source data.In addition,according to the geological background and conditions in Da’anshan coal mine in western Beijing,China,a preliminary attempt is made to reconstruct a digital model of fault and fold structures using the methodology proposed in this study.展开更多
基金jointly supported by the National Natural Science Foundation of China U1901602,U2239252)the National Key R&D Program of China(No.2019YFE0115700)+1 种基金the Scientific Research Fund of Institute of Engineering Mechanics,China Earthquake Administration(Grant No.2021EEEVL0202)the Natural Science Foundation of Heilongjiang Province(LH2020E021)。
文摘When evaluating an area's seismic risk or resilience,it is necessary to use the spatial correlation to analyze the ground motion parameters of multiple sites together in an earthquake.These two large earthquakes in Türkiye provided the possibility for spatial correlation analysis of ground motion intensity measurements in this area.Based on the strong motion records provided by The Disaster and Emergency Management Authority of Türkiye(AFAD),this study uses the local ground motion prediction equation in Türkiye to give spatial correlation analysis of Intensity Measurements.This study gives an exponential model based on a semivariogram and compares it with the correlation model obtained from previous studies.
基金supported by the National Key Research and Development Program of China(Grant No.2021YFA1201100)the National Natural Science Foundation of China(Grant Nos.82103006,82030092,81720108028,82072657,82072716,82103003,82173295,81871968,81871978,82072691,and 82103222)+1 种基金the Tianjin Hygiene Healthy Science and Technology Project(Grant No.TJWJ2022MS007)the Science&Technology Development Fund of Tianjin Education Commission for Higher Education(Grant No.2020KJ141).
文摘Objective:Pancreatic ductal adenocarcinoma(PDAC)is a highly malignant gastrointestinal cancer with a 5-year survival rate of only 9%.Of PDAC patients,15%-20%are eligible for radical surgery.Gemcitabine is an important chemotherapeutic agent for patients with PDAC;however,the efficacy of gemcitabine is limited due to resistance.Therefore,reducing gemcitabine resistance is essential for improving survival of patients with PDAC.Identifying the key target that determines gemcitabine resistance in PDAC and reversing gemcitabine resistance using target inhibitors in combination with gemcitabine are crucial steps in the quest to improve survival prognosis in patients with PDAC.Methods:We constructed a human genome-wide CRISPRa/dCas 9 overexpression library in PDAC cell lines to screen key targets of drug resistance based on sgRNA abundance and enrichment.Then,co-IP,ChIP,ChIP-seq,transcriptome sequencing,and qPCR were used to determine the specific mechanism by which phospholipase D1(PLD1)confers resistance to gemcitabine.Results:PLD1 combines with nucleophosmin 1(NPM1)and triggers NPM1 nuclear translocation,where NPM1 acts as a transcription factor to upregulate interleukin 7 receptor(IL7R)expression.Upon interleukin 7(IL-7)binding,IL7R activates the JAK1/STAT5 signaling pathway to increase the expression of the anti-apoptotic protein,BCL-2,and induce gemcitabine resistance.The PLD1 inhibitor,Vu0155069,targets PLD1 to induce apoptosis in gemcitabine-resistant PDAC cells.Conclusions:PLD1 is an enzyme that has a critical role in PDAC-associated gemcitabine resistance through a non-enzymatic interaction with NPM1,further promoting the downstream JAK1/STAT5/Bcl-2 pathway.Inhibiting any of the participants of this pathway can increase gemcitabine sensitivity.
基金supported by the National Natural Science Foundation of China(32030081,31871610)the Agricultural Variety Improvement Project of Shandong Province(2019LZGC016)the U.S.Wheat and Barley Scab Initiative。
文摘Fhb7 is a major gene that was transferred from Thinopyrum ponticum to chromosome 7D of wheat(Triticum aestivum)and confers resistance to both Fusarium head blight(FHB)and Fusarium crown rot(FCR).However,Fhb7 is tightly linked to the PSY-E2 gene,which causes yellow flour,limiting its application in breeding.To break this linkage,marker K-PSY was developed for tagging PSY-E2 and used with Fhb7 markers to identify recombination between the two genes.Screening 21,000 BC1F2 backcross progeny(Chinese Spring ph1bph1b*2/SDAU 2028)revealed two Fhb7^(+)wheat-Tp7el_(2)L lines,Shannong 2–16and Shannong 16–1,that carry a desired truncated Fhb7^(+)translocation segment without PSY-E2.The two lines show levels of resistance to FHB and FCR similar to those of the original translocation line SDAU 2028,but have white flour.To facilitate Fhb7 use in wheat breeding,STS markers were developed and used to isolate Fhb7 on a truncated Tp7el_(2) translocation segment.Near-isogenic lines carrying the Fhb7^(+)segment were generated in the backgrounds of three commercial cultivars,and Fhb7^(+)lines showed increased FHB and FCR resistance without yield penalty.The breakage of the tight linkage between Fhb7 and PSY-E2 via homoeologous recombination provides genetic resources for improvement of wheat resistance to FHB and FCR and permit the large-scale deployment of Fhb7 in breeding using marker-assisted selection.
基金supported by the State Key Research Development Program of China(2022YFC3004602)Independent Research fund of Joint NationalLocal Engineering Research Centre for Safe and Precise Coal Mining(Anhui University of Science and Technology)(EC2022001)+2 种基金the National Natural Science Foundation of China(41872205)Beijing Natural Science Foundation(8202041)the Fundamental Research Funds for the Central Universities(2022YJSLJ08,2022JCCXNY03).
文摘In order to comprehend the dynamic disaster mechanism induced by overburden rock caving during the advancement of a coal mining face, a physical simulation model is constructed basing on the geological condition of the 21221 mining face at Qianqiu coal mine in Henan Province, China. This study established, a comprehensive monitoring system to investigate the interrelations and evolutionary characteristics among multiple mechanical parameters, including mining-induced stress, displacement, temperature, and acoustic emission events during overburden rock caving. It is suggested that, despite the uniformity of the overburden rock caving interval, the main characteristic of overburden rock lies in its uneven caving strength. The mining-induced stress exhibits a reasonable interrelation with the displacement, temperature, and acoustic emission events of the rock strata. With the advancement of the coal seam, the mining-induced stress undergoes four successive stages: gentle stability, gradual accumulation, high-level mutation, and a return to stability. The variations in other mechanical parameters does not synchronize with the signifcant changes in mining-induced stress. Before the collapse of overburden rock occurs, rock strata temperature increment decreases and the acoustic emission ringing counts surges with the increase of rock strata displacement and mining-induced stress. Therefore, the collaborative characteristics of mining-induced stress, displacement, temperature, and acoustic emission ringing counts can be identifed as the precursor information or overburden rock caving. These results are in good consistent with on-site situation in the coal mine.
基金supported by the National Key Research and Development Program of China(2021YFD1200703)Beijing Outstanding Young Scientist Program(BJJWZYJH01201910019026)the National Natural Science Foundation of China(32171940)。
文摘Reliance on agriculture for food security is a constant in all modern societies.Global climate change and population growth have put immense pressure on sustainable agriculture,exacerbating the effects of environmental stresses.Drought is one of the most pressing abiotic stresses that farmers face,presenting an annual threat to crop growth and yield.Crops have evolved extensive morphological,physiological,and molecular mechanisms to combat drought stress.Drought resistance is a polygenic trait,controlled by a complex genetic network and an array of genes working together to ensure plant survival.Many studies have aimed at dissecting the genetic mechanisms underlying drought resistance.Recent studies using linkage and association mapping have made progress in identifying genetic variations that affect drought-resistance traits.These loci may potentially be engineered by genetic transformation and genome editing aimed at developing new,stress-resistant crop cultivars.Here we summarize recent progress in elucidating the genetic basis of crop drought resistance.Molecular-breeding technologies such as marker-assisted selection,genome selection,gene transformation,and genome editing are currently employed to develop drought-resistant germplasm in a variety of crops.Recent advances in basic research and crop biotechnology covered in this review will facilitate delivery of drought-resistant crops with unprecedented efficiency.
基金supported by the National Natural Science Foundation of China (U1834211, 61925302, 62103033)the Open Research Fund of the State Key Laboratory for Management and Control of Complex Systems (20210104)。
文摘High-speed rail(HSR) has formed a networked operational scale in China. Any internal or external disturbance may deviate trains’ operation from the planned schedules, resulting in primary delays or even cascading delays on a network scale. Studying the delay propagation mechanism could help to improve the timetable resilience in the planning stage and realize cooperative rescheduling for dispatchers. To quickly and effectively predict the spatial-temporal range of cascading delays, this paper proposes a max-plus algebra based delay propagation model considering trains’ operation strategy and the systems’ constraints. A double-layer network based breadth-first search algorithm based on the constraint network and the timetable network is further proposed to solve the delay propagation process for different kinds of emergencies. The proposed model could deal with the delay propagation problem when emergencies occur in sections or stations and is suitable for static emergencies and dynamic emergencies. Case studies show that the proposed algorithm can significantly improve the computational efficiency of the large-scale HSR network. Moreover, the real operational data of China HSR is adopted to verify the proposed model, and the results show that the cascading delays can be timely and accurately inferred, and the delay propagation characteristics under three kinds of emergencies are unfolded.
基金supported by the Natural Science Foundation of Guangdong Province(Grant No.2020A151501287)the General Project of Science and Technology Innovation Commission of Shenzhen(Grant Nos.JCYJ20210324134800001,JCYJ20190808103401655)+1 种基金Basic Public Welfare Research Project of Zhejiang Province(Grant No.LGF21H090011)the National Natural Science Foundation of China(Grant No.82174132).
文摘Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury(CIRI).The exact mechanisms underlying I/R injury are unclear.In this study,we aimed to determine the role of m6A-modified methylase complex methyltransferase-like 3(METTL3)in cerebral ischemiareperfusion(I/R)injury.We found that m6A and METTL3 levels increased in OGD/RX-induced mouse astrocytescerebellar(MA-C)and the brain of middle cerebral artery occlusion(MCAO)model mice.METTL3 siRNA treatment reduced OGD-RX-induced MAC cell viability and proliferation,which increased with METTL3 over-expression.Flow cytometry analysis showed that silencing METTL3 significantly enhanced OGD/RX-induced MAC apoptosis,which was significantly reduced with METTL3 up-regulation.In an MCAO model,METTL3 overexpression significantly reduced cerebral infarction area and decreased brain cell apoptosis,indicating that METTL3 OE treatment could ameliorate brain edema and injury.Thus,METTL3 could be used as a target to treat I/R injury.
文摘With the rapid development of railway transportation,higher requirements for capacity are increasing and amount of communication,computer and control technologies are applied in train control systems which is the popular method for train control.The application of 3 C brings the enhancement of railway transportation performance.However,the number of internal and external cyber security threats is rising,which could lead to some railway accidents.On the other hand,safety is the core of the design process for railway but security is rarely considered due to the closure of traditional railway systems.As cyber security is threatening the normal operation of industrial control systems and the critical infrastructure,security ssues of train control systems should be paid more attention as railway transportation play an important role for society and economy.However,due to the safety-critical characteristics,safety assessment and analysis works have been performed for a long time,but cyber security related works are rarely considered.In the paper,we demonstrate the security situation of train control systems based on the inherent features and the experience of other industrial control systems,where the technical defects and potential threats are summarized.According to the practical engineering experience,the current security protection strategies are illustrated,which shows the popular security protection methods are limited and cannot realize the defense-in-depth.Finally,some research challenges of security issues of train control systems are identified.
基金financially supported by the National Natural Science Foundation of China(No.41502184)Beijing Natural Science Foundation(No.2164067)+2 种基金National Key Research and Development Program(No.2016YFC0801401)Fundamental Research Funds for the Central Universities(No.2014QL01)Innovation Training Programs for Undergraduate Students(Nos.201411413054 and SKLCRSM14CXJH08)
文摘This paper presents an investigation on the characteristics of overlying strata collapse and mininginduced pressure in fault-influenced zone by employing the physical modeling in consideration of fault structure.The precursory information of fault slip during the underground mining activities is studied as well.Based on the physical modeling,the optimization of roadway support design and the field verification in fault-influenced zone are conducted.Physical modeling results show that,due to the combined effect of mining activities and fault slip,the mining-induced pressure and the extent of damaged rock masses in the fault-influenced zone are greater than those in the uninfluenced zone.The sharp increase and the succeeding stabilization of stress or steady increase in displacement can be identified as the precursory information of fault slip.Considering the larger mining-induced pressure in the fault-influenced zone,the new support design utilizing cables is proposed.The optimization of roadway support design suggests that the cables can be anchored in the stable surrounding rocks and can effectively mobilize the load bearing capacity of the stable surrounding rocks.The field observation indicates that the roadway is in good condition with the optimized roadway support design.
基金supported by the Aeronautical Science Fund of China(201455960252015209619)
文摘To enhance the capacity of the radar-reconnaissance interception receiver recognizing linear frequency modulated(LFM) at a low signal-noise ratio, this paper presents WignerHough transform(WHT) of the LFM signal and its corresponding characteristics, derives the probability density functions of the LFM signal and Gaussian white noise within WHT based on entropy(WHTE), dimension under different assumptions and puts forward a WHT algorithm based on entropy of slice to improve the capacity of detecting the LFM signal. Entropy of the WHT domain slice is adopted to assess the information size of polar radius or angle slice, which is converted into the weight factor to weight every slice. Double-deck weight is used to weaken the influences of noise and disturbance terms and WHTE treatment and signal detection procedure are also summarized. The rationality of the algorithm is demonstrated through theoretical analysis and formula derivation, the efficiency of the algorithm is verified by simulation comparison between WHT, fractional Fourier transform and periodic WHT, and it is highlighted that the WHTE algorithm has better detection accuracy and range of application against strong noise background.
基金supported by the National Key Research and Development Program (Grant No. 2016YFC0801401)National Natural Science Foundation of China (Grant No. 41502184)Beijing Natural Science Foundation (Grant No. 2164067)
文摘Coal bump refers to a sudden catastrophic failure of coal seam and usually can cause serious damages to underground mining facilities and staff. In this circumstance, this paper focuses on the recent achievements in the mechanism and prevention techniques of coal bumps over the past five years in China.Based on theoretical analysis, laboratory experiment, numerical simulation and field test, the characteristics of coal bumps occurrence in China's coal mines were described, and the difference between coal bumps and rockbursts was also discussed. In addition, three categories of coal bumps induced by"material failure" were introduced, i.e. hard roof, floor strata and tectonic structures, in which the mechanism of coal bumps induced by geological structures was analyzed. This involves the bump liability and microstructure effects on bump-prone coal failure, the mechanism of coal bumps in response to fault reactivation, island face mining or hard roof failure. Next, the achievements in the monitoring and controlling methods of coal bumps were reviewed. These methods involve the incorporated prediction system of micro-seismicity and mining-induced pressure, the distributed micro-seismic monitoring system, energy absorption support system, bolts with constant resistance and large elongation,and the "multi-stage" high-performance support. Finally, an optimal mining design is desirable for the purpose of coal bump mitigation.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences, China (Grant No. XDA20100101)the Foundation for Excellent Youth Scholars of Northwest Institute of Eco-environment and Resources, Chinese Acad emy of Sciences (Y651K91001)
文摘This research was undertaken to clarify the characteristics of vegetation change and its main influencing factors on the Qinghai-Tibet Plateau. Using the greenness rate of change(GRC) and correlation factors, we analyzed the trend of vegetation change and its dominant factors from 2000 to 2015. The results indicate that the vegetation tended to improve from 2000 to 2015 on the Qinghai-Tibet Plateau, with the improved area accounting for 39.93% of the total; and the degraded area accounting for 19.32%. The areas of degraded vegetation are mainly concentrated in the low-relief and intermediate-relief mountains of the high-altitude and extremely high-altitude areas on the Qinghai-Tibet Plateau, as the vegetation characteristics are impacted by the terrain. Temperature and precipitation have obvious response mechanisms to vegetation growth, but the effects of precipitation and temperature on vegetation degradation are not significant over a short time frame. Overgrazing and population growth are the dominant factors of vegetation degradation on the Qinghai-Tibet Plateau.
基金supported by the Science Foundation of Institute of Engineering Mechanics, China Earthquake Administration (CEA) under Grant No. 2014B06the National Natural Science Foundation of China Nos. 51308515 and 51278473
文摘The Ludian County of Yunnan Province in southwestern China was struck by an MS6.5 earthquake on August 3, 2014, which was another destructive event following the MS8.0 Wenchuan earthquake in 2008, MS7.1 Yushu earthquake in 2010, and MS7.0 Lushan earthquake in 2013. National Strong-Motion Observation Network System of China collected 74 strong motion recordings,which the maximum peak ground acceleration recorded by the 053 LLT station in Longtoushan Town was 949 cm/s2 in E–W component. The observed PGAs and spectral ordinates were compared with ground-motion prediction equation in China and the NGA-West2 developed by Pacific Earthquake Engineering Researcher Center. This earthquake is considered as the first case for testing applicability of NGA-West2 in China. Results indicate that the observed PGAs and the 5 % damped pseudo-response spectral accelerations are significantly lower than the predicted ones. The field survey around some typical strong motion stations verified that the earthquake damage was consistent with the official isoseismal by China Earthquake Administration.
基金Our work is supported by the Natural Science Fund of Jiangsu Province(BK20001414).
文摘In this paper, diamond-like carbon (DLC) fi[ms were deposited on Ti alloy by electro-deposition. DLC films were brown and composed of the compact grains whose diameter was about 400 nm. Examined by XPS, the main composition of the films was carbon. In the Raman spectrum, there were a broad peak at 1350 cm-1 and a broad peak at 1600 cm-1, which indicated that the films were DLC films.
基金supported by the National Natural Science Foundation of China(6147322711472222)+2 种基金the Aerospace Technology Support Fund of China(2014-HT-XGD)the Natural Science Foundation of Shaanxi Province(2015JM6304)the Aeronautical Science Foundation of China(20151353018)
文摘A new Gaussian approximation nonlinear filter called generalized cubature quadrature Kalman filter(GCQKF) is introduced for nonlinear dynamic systems. Based on standard GCQKF,two extensions are developed, namely square root generalized cubature quadrature Kalman filter(SR-GCQKF) and iterated generalized cubature quadrature Kalman filter(I-GCQKF). In SR-GCQKF,the QR decomposition is exploited to alter the Cholesky decomposition and both predicted and filtered error covariances have been propagated in square root format to make sure the numerical stability. In I-GCQKF, the measurement update step is executed iteratively to make full use of the latest measurement and a new terminal criterion is adopted to guarantee the increase of likelihood. Detailed numerical experiments demonstrate the superior performance on both tracking stability and estimation accuracy of I-GCQKF and SR-GCQKF compared with GCQKF.
文摘It is generally recognized that Caucasians and Asians have different skin aging features.The aim of this study was to develop a facial wrinkle grading scale for Chinese women.Standard photographs were taken of 242 Chinese women.Six sets of 0 to 9 wrinkle scales with reference photographs and descriptions were selected,including grading scales for resting and hyperkinetic crow's feet,frontalis lines,glabellar frown lines,and nasolabial folds.To identify the scale by objective quantitative measurement,skin surface measurements from the Visioscan~ VC98 were used.To test the reliability and validity of our wrinkle scale,a multi-rater consensus method was used.A double-blind,randomized,vehicle-controlled 12-week study was conducted to use this clinical photo-score to evaluate the efficacy and safety of Centella triterpenes cream~ in treating crow's feet.A newly developed 10-point photographic and descriptive scale emerged from this study.The final atlas of these photographs contained a total of 6 sets with 10 pictures each.From 0 to 9,surface evaluation of smoothness(SEsm) parametric measurements decreased progressively,indicating that the scale increased inversely.Weighted kappa coefficients for intra-assessor were between 0.75-0.87.The overall Kendall's coefficient is 0.86 on the first rating and 0.87 on the second rating.Thirtysix volunteers were recruited and 35 subjects completed a 12-week trial.Clinical photo-score by investigator showed a significant difference(P < 0.05) between the treatment side and control side after 4 weeks.Use of these scales in clinical settings to evaluate facial wrinkles in Asians individuals is recommended.
基金supported by the National Natural Science Foundation of China(Grant No.51509046)Foundation of Ministry of Industry and Information Technology High-tech Ship Scientific Research(Grant No.2016-26)
文摘In response to the development of deep-sea oil and gas resources,which require a high degree of cooperation by crude oil transportation equipment,a new type of ship known as the cargo transfer vessel(CTV)has been developed.To provide a theoretical reference for the design and equipment of the CTV’s dynamic positioning system,in this paper,we take the new deepwater CTVas the study object and theoretically and numerically analyze its operation,wind load,current load,wave load,and navigational resistance in a range of Brazilian sea conditions with respect to its positioning and towing modes.We confirm that our proposed method can successfully calculate the total environmental load of the CTVand that the CTV is able to operate normally under the designed sea conditions.
基金financial support from the projects of the National Natural Science Foundation of China(NSFC)(Grant Nos.51809259,51774056,and 51774095)the CAS Pioneer Hundred Talents Program in China。
文摘It is well known that the complicated channeling of fluid flow and heat transfer is strongly related with the intricate natural fracture system.However,it is still challenging to set up the fracture network model which is strong heterogeneous.Compared with other methods(e.g.equivalent continuum model(ECM),discrete fracture model(DFM),and ECM-DFM),the fracture flow module in the COMSOL Multiphysics simulator is powerful in definition of fractures as the inner flow boundary existing in the porous media.Thus it is selected to simulate the fluid flow and heat transfer in the geothermal-developed fractured granite of Sanguliu area located at Liaodong Peninsula,Eastern China.The natural faults/fractures based on field investigation combined with the discrete fracture network(DFN)generated by the MATLAB are used to represent the two-dimensional geological model.Numerical results show that early thermal breakthrough occurs at the production well caused by quick flow of cold water along the highly connected fractures.Suitable hydraulic fracturing treatments with proper injection rates,locations,etc.can efficiently hinder the thermal breakthrough time in the natural fracture system.Large well spacing helps the long-term operation of geothermal production,but it is highly dependent on the geometrical morphology of the fracture network.The enhancement of reservoir properties at the near-well regions can also increase the geothermal production efficiency.The results in this study can provide references to achieve a sustainable geothermal exploitation in fractured granitic geothermal reservoirs or hot dry rocks at depth.
基金This study was financially supported by the Beijing Natural Science Foundation(8202041)National Natural Science Foundation of China(41872205)+1 种基金Yue Qi Young Scholar Project,China University of Mining&Technology,Beijing(2018QN13)the Fundamental Research Funds for the Central Universities(2022YJSLJ08,2021YJSLJ10).
文摘Visualization of complex geological structures can technically support the accurate prediction and prevention of coal mine disasters.This study proposed a new digital reconstruction method to visualize geological structures based on establishing a virtual model in the digital twin system.This methodology for the digital reconstruction of complex fault structures comprises the following four aspects:(1)collection and fdelity of multi-physical feld data of the fault structures,(2)the transmission of multi-physical feld data,(3)the normalization of multi-physical feld data,and(4)digital model reconstruction of fault structures.The key scientifc issues of this methodology to be resolved include in situ fdelity of multi-feld data and normalized programming of multi-source data.In addition,according to the geological background and conditions in Da’anshan coal mine in western Beijing,China,a preliminary attempt is made to reconstruct a digital model of fault and fold structures using the methodology proposed in this study.
