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Methylation profiling of twenty four genes and the concordant methylation behaviours of nineteen genes that may contribute to hepatocellular carcinogenesis 被引量:34
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作者 JIANYU hongyuzhang +3 位作者 ZHENZHONGMA WEILU YIFEIWANG JINGDEZHU 《Cell Research》 SCIE CAS CSCD 2003年第5期319-333,共15页
To determine the possible role of the epigenetic mechanisms in carcinogenesis of the hepatocellular carcinoma, we methylation-profiled the promoter CpG islands of twenty four genes both in HCC tumors and the neighbori... To determine the possible role of the epigenetic mechanisms in carcinogenesis of the hepatocellular carcinoma, we methylation-profiled the promoter CpG islands of twenty four genes both in HCC tumors and the neighboring non-cancerous tissues of twenty eight patients using the methylation-specific PCR (MSP) method in conjunction with the DNA sequencing. In comparison with the normal liver tissues from the healthy donors, it was found that while remained unmethylated the ABL, CAV, EPO, GATA3, LKB1, NEP, NFL, NIS and p27^(KIP1) genes, varying extents of the HCC specific hypermethylation were found associated with the ABO, AR, CSPG2, cyclin al, DBCCR1, GALR2, IRF7, MGMT, MT1A, MYOD1, OCT6, p57^(KIP2), p73, WT1 genes, and demethylation with the MAGEA1 gene, respectively. Judged by whether the hypermethylated occurred in HCC more frequently than in their neighboring normal tissues, the hypermethylation status of the AR, DBCCR1, IRF7, OCT6, and p73 genes was considered as the event specific to the late stage, while that the rest that lacked such a distinguished contrast, as the event specific to the early stage of HCC carcinogenesis. Among all the clinical pathological parameters tested for the association with, the hypermethylation of the cyclin al gene was more prevalent in the non-cirrhosis group (P=0.021) while the hypermethylated p16^(INK4a) gene was more common in the cirrhosis group (P=0.017). The concordant methylation behaviors of nineteen genes, including the four previously studied and their association with cirrhosis has been evaluated by the best subgroup selection method. The data presented in this report would enable us to shape our understanding of the mechanisms for the HCC specific loss of the epigenetic stability of the genome, as well as the strategy of developing the novel robust methylation based diagnostic and prognostic tools. 展开更多
关键词 promoter CpG island methylation specific PCR concordant behaviors of methylation.
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1, 4-Pyrone Effects on O-H Bond Dissociation Energies of Catechols in Flavonoids: A Density Functional Theory Study
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作者 hongyuzhang YouMinSUN 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第6期531-534,共4页
关键词 ANTIOXIDANT density functional theory flavonoid O-H bond dissociation energy structure-activity relationships.
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EGF receptor-mediated intracellular calcium increasein human hepatoma BEL-7404 cells
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作者 FU TAO YONGHUA XU +3 位作者 WANLI JIANG hongyuzhang PEIHONG ZHU JUN WU(Labomtory of Cellular and Molecular Oncology, Shanghai Institute of Cell Biology)(Shanghai Institute of Physiology, Chinese Academy of Sciences, Shanghai 200031,China) 《Cell Research》 SCIE CAS CSCD 1994年第2期145-153,共9页
Epidermal growth factor (EGF) induced intracellular free calcium ([Ca2+]i) response was studied in fura-2- or fluo-3-loaded human hepatoma cells of BEL-7404 cell line. Single cell [Ca2+]i analysis and [Ca2+], measur... Epidermal growth factor (EGF) induced intracellular free calcium ([Ca2+]i) response was studied in fura-2- or fluo-3-loaded human hepatoma cells of BEL-7404 cell line. Single cell [Ca2+]i analysis and [Ca2+], measurement in cell populations revealed that EGF triggered a rapid [Ca2+]iincrease in the dose-dependent and time- dependent manner. Pretreatment of cells with an endoplasmic reticulum (ER) Ca2+-ATPase inhibitor, thapsigargin (TG) at 100 nM concentration for 20 min, completely abolished EGF-induced [Ca2+]i increase, and chelating extracellular calcium by excess EGTA partially inhibited the increase.Furthermore, the expression of antisense EGF receptor sequence in BEL-7404 cells suppressed the [Ca2+]i response to EGF. The results suggest that EGF receptor-mediated [Ca2+]i increase in the human hepatoma cells is essentially dependent on the Ca2+ storage in ER. 展开更多
关键词 EGF receptor CALCIUM THAPSIGARGIN human hepatoma cells
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Theoretical Investigation on Radical-Coupling Reactivity of Indolinonic Aminoxyls
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作者 hongyuzhang LanFenWANG 《Chinese Chemical Letters》 SCIE CAS CSCD 2003年第4期437-440,共4页
Indolinonic aminoxyls can effectively scavenge various radicals by directly coupling with them or by imitating superoxide dismutase. To better understand the radical-coupling reactions, DFT method B3LYP/6-31G(d,p) wa... Indolinonic aminoxyls can effectively scavenge various radicals by directly coupling with them or by imitating superoxide dismutase. To better understand the radical-coupling reactions, DFT method B3LYP/6-31G(d,p) was employed to calculate variations of free energy for the coupling reactions and other physico-chemical parameters. The radical-coupling activity difference between aminoxyls was elucidated to a large extent in terms of electronic properties of substituents. 展开更多
关键词 Density functional theory indolinonic aminoxyl radical-coupling reaction.
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