Forty-eight male Lezhi black goat kids with similar body weight((12.09±1.70)kg)and age((60±5)d)were used to determine the effect of dietary copper(Cu),in the form of reagent grade Cu sulfate(CuSO4∙5H2O),on p...Forty-eight male Lezhi black goat kids with similar body weight((12.09±1.70)kg)and age((60±5)d)were used to determine the effect of dietary copper(Cu),in the form of reagent grade Cu sulfate(CuSO4∙5H2O),on performance,serum lipid profile,and the relative mRNA abundance of genes involved in lipid metabolism.Goat kids were stratified by body weight and randomly assigned to one of 4 treatment groups.Each treatment consisted of 12 replicate pens with each pen containing one goat kid.Treatment groups received the basal diet with no supplemental Cu(control),basal diet plus 10 mg of Cu kg^(-1)of dry matter(DM),basal diet plus 20 mg of Cu kg^(-1)of DM,or basal diet plus 30 mg of Cu kg^(-1)of DM.Goats were housed individually in pens and fed a high-concentrate pelleted diet for 60 d.Average daily gain,average daily feed intake and feed:gain of goats were not affected by dietary Cu supplementation(P>0.10).No differences were detected in serum total cholesterol,triglyceride,and high density lipoprotein cholesterol concentrations of goat kids fed with different Cu concentrations(P>0.05).However,serum low density lipoprotein cholesterol concentrations decreased linearly(P=0.01)as the concentration of dietary Cu increased.Intramuscular fat content of longissimus muscle increased(P=0.002)quadratically and liver Cu concentrations increased(P<0.001)linearly as dietary Cu concentration increased.Compared with the control,dietary supplementation of 20 mg Cu kg^(-1)DM decreased the relative mRNA abundance of fatty acid-binding protein 4(P=0.01)and lipoprotein lipase(P=0.05),and tended to decrease the relative mRNA abundance of carnitine palmitoyltransferase I(P=0.06)in longissimus muscle of goats.The relative mRNA abundance of peroxisome proliferator-activated receptor alpha(P<0.001),carnitine acetyltransferase(P=0.001),and carnitine palmitoyltransferase Ⅰ(P=0.001)were also decreased in liver by Cu supplementation.These results indicate that dietary supplementation of Cu modified lipid metabolism by increasing muscular fat and decreasing serum low density lipoprotein cholesterol,and the modification might be associated with the reduction of relative mRNA abundance of genes for oxidation of long-chain fatty acid in muscle and liver of Lezhi black goat kids.展开更多
“主客”网络交互行为是国内外重要的研究课题。文章运用CiteSpace v5.0 R7软件对Web of Science核心合集2000-2020年网络交互行为文献进行量化分析,探究社会科学的研究热点、演变趋势及其对旅游研究的启示。研究发现:(1)国外社会科学...“主客”网络交互行为是国内外重要的研究课题。文章运用CiteSpace v5.0 R7软件对Web of Science核心合集2000-2020年网络交互行为文献进行量化分析,探究社会科学的研究热点、演变趋势及其对旅游研究的启示。研究发现:(1)国外社会科学基于多学科理论视角,核心学科主要从社会学、心理学、经济学,交叉学科从社会心理学、人类学、计算机科学等,新进学科从传播学、生理心理学等学科对网络交互行为进行了研究。(2)旅游理论研究主要聚焦于社会学和心理学,缺乏对传播学、计算机科学等领域理论的运用;研究方法以定量为主,缺少利用大数据方法对多源异构数据进行定性和定量的混合分析。(3)社会科学研究主题集中在网络健康、电子商务、虚拟交往、网络生态环境行为;旅游研究更关注旅游后的信息分享行为。展开更多
当前食源性致病菌导致的食品安全事故频发,特别是大肠杆菌O157:H7,通过合成NH_2-Fe_3O_4磁性颗粒,利用其带电性对大肠杆菌O157:H7进行富集,在优化富集条件的基础上,通过利用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser des...当前食源性致病菌导致的食品安全事故频发,特别是大肠杆菌O157:H7,通过合成NH_2-Fe_3O_4磁性颗粒,利用其带电性对大肠杆菌O157:H7进行富集,在优化富集条件的基础上,通过利用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)对其进行检测,验证该方法的检测限为3.5×10~3 CFU/m L,同时对模拟实际样本(碳酸饮料)中的大肠杆菌O157:H7进行检测,并搜索核糖体蛋白数据库鉴别了大肠杆菌O157:H7。展开更多
基金supported by the National Natural Science Foundation of China(31501977)the Sichuan Provincial Key R&D Project,China(22ZDYF0194)+1 种基金the Fundamental Research Funds for the Central Universities of Southwest Minzu University(2021PTJS20)the Innovation Team Development Funds for Sichuan Mutton Sheep,China(cxtd2019–14).
文摘Forty-eight male Lezhi black goat kids with similar body weight((12.09±1.70)kg)and age((60±5)d)were used to determine the effect of dietary copper(Cu),in the form of reagent grade Cu sulfate(CuSO4∙5H2O),on performance,serum lipid profile,and the relative mRNA abundance of genes involved in lipid metabolism.Goat kids were stratified by body weight and randomly assigned to one of 4 treatment groups.Each treatment consisted of 12 replicate pens with each pen containing one goat kid.Treatment groups received the basal diet with no supplemental Cu(control),basal diet plus 10 mg of Cu kg^(-1)of dry matter(DM),basal diet plus 20 mg of Cu kg^(-1)of DM,or basal diet plus 30 mg of Cu kg^(-1)of DM.Goats were housed individually in pens and fed a high-concentrate pelleted diet for 60 d.Average daily gain,average daily feed intake and feed:gain of goats were not affected by dietary Cu supplementation(P>0.10).No differences were detected in serum total cholesterol,triglyceride,and high density lipoprotein cholesterol concentrations of goat kids fed with different Cu concentrations(P>0.05).However,serum low density lipoprotein cholesterol concentrations decreased linearly(P=0.01)as the concentration of dietary Cu increased.Intramuscular fat content of longissimus muscle increased(P=0.002)quadratically and liver Cu concentrations increased(P<0.001)linearly as dietary Cu concentration increased.Compared with the control,dietary supplementation of 20 mg Cu kg^(-1)DM decreased the relative mRNA abundance of fatty acid-binding protein 4(P=0.01)and lipoprotein lipase(P=0.05),and tended to decrease the relative mRNA abundance of carnitine palmitoyltransferase I(P=0.06)in longissimus muscle of goats.The relative mRNA abundance of peroxisome proliferator-activated receptor alpha(P<0.001),carnitine acetyltransferase(P=0.001),and carnitine palmitoyltransferase Ⅰ(P=0.001)were also decreased in liver by Cu supplementation.These results indicate that dietary supplementation of Cu modified lipid metabolism by increasing muscular fat and decreasing serum low density lipoprotein cholesterol,and the modification might be associated with the reduction of relative mRNA abundance of genes for oxidation of long-chain fatty acid in muscle and liver of Lezhi black goat kids.
文摘“主客”网络交互行为是国内外重要的研究课题。文章运用CiteSpace v5.0 R7软件对Web of Science核心合集2000-2020年网络交互行为文献进行量化分析,探究社会科学的研究热点、演变趋势及其对旅游研究的启示。研究发现:(1)国外社会科学基于多学科理论视角,核心学科主要从社会学、心理学、经济学,交叉学科从社会心理学、人类学、计算机科学等,新进学科从传播学、生理心理学等学科对网络交互行为进行了研究。(2)旅游理论研究主要聚焦于社会学和心理学,缺乏对传播学、计算机科学等领域理论的运用;研究方法以定量为主,缺少利用大数据方法对多源异构数据进行定性和定量的混合分析。(3)社会科学研究主题集中在网络健康、电子商务、虚拟交往、网络生态环境行为;旅游研究更关注旅游后的信息分享行为。
文摘当前食源性致病菌导致的食品安全事故频发,特别是大肠杆菌O157:H7,通过合成NH_2-Fe_3O_4磁性颗粒,利用其带电性对大肠杆菌O157:H7进行富集,在优化富集条件的基础上,通过利用基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS)对其进行检测,验证该方法的检测限为3.5×10~3 CFU/m L,同时对模拟实际样本(碳酸饮料)中的大肠杆菌O157:H7进行检测,并搜索核糖体蛋白数据库鉴别了大肠杆菌O157:H7。
