AIM:To investigate apparent diffusion coefficient (ADC) values as an indication of reconditioning of acute hepatic injury (AHI) after allogeneic mononuclear bone marrow cell (MBMC) transplantation.METHODS:Three groups...AIM:To investigate apparent diffusion coefficient (ADC) values as an indication of reconditioning of acute hepatic injury (AHI) after allogeneic mononuclear bone marrow cell (MBMC) transplantation.METHODS:Three groups were used in our study:a cell transplantation group (n=21),transplantation control group (n=21) and normal control group (n=10).AHI model rabbits in the cell transplantation group were injected with 5 mL of MBMC suspension at multiple sites in the liver and the transplantation controls were injected with 5 mL D-Hanks solution.At the end of the 1st,2nd and 4th wk,7 rabbits were randomly selected from the cell transplantation group and transplantation control group for magnetic resonance diffusion-weighted imaging (MR-DWI) and measurement of the mean ADC values of injured livers.After MR-DWI examination,the rabbits were sacrificed and the livers subjected to pathological examination.Ten healthy rabbits from the normal control group were used for MRDWI examination and measurement of the mean ADC value of normal liver.RESULTS:At all time points,the liver pathological scores from the cell transplantation group were significantly lower than those in the transplantation control group (27.14±1.46 vs 69.29±6.16,22.29±2.29 vs 57.00±1.53,19.00±2.31vs 51.86±6.04,P=0.000).The mean ADC values of the cell transplantation group were significantly higher than the transplantation control group ((1.07±0.07)×10-3 mm2/svs (0.69±0.05)×10-3 mm2/s,(1.41±0.04)×10-3 mm2/s vs (0.84±0.06)×10-3 mm2/s,(1.68±0.04)×10-3 mm2/svs (0.86±0.04)×10-3 mm2/s,P=0.000).The pathological scores of the cell transplantation group and transplantation control group gradually decreased.However,their mean ADC values gradually increased to near that of the normal control.At the end of the 1st wk,the mean ADC values of the cell transplantation group and transplantation control group were significantly lower than those of the normal control group [(1.07±0.07)×10-3 mm2/s vs (1.76±0.03)×10-3 mm2/s,(0.69±0.05)×10-3 mm2/s vs (1.76±0.03)×10-3 mm2/s,P=0.000].At any 2 time points,the pathological scores and the mean ADC values of the cell transplantation group were significantly different (P=0.000).At the end of the 1st wk,the pathological scores and the mean ADC values of the transplantation control group were significantly different from those at the end of the 2nd and 4th wk (P=0.000).However,there was no significant difference between the 2nd and 4th wk (P=0.073 and 0.473,respectively).The coefficient of correlation between the pathological score and the mean ADC value in the cell transplantation group was-0.883 (P=0.000) and-0.762 (P=0.000) in the transplantation control group.CONCLUSION:Tracking the longitudinally dynamic change in the mean ADC value of the AHI liver may reflect hepatic injury reconditioning after allogeneic MBMC transplantation.展开更多
AIM: To develop a potent and safe gene therapy for esophageal cancer.METHODS: An expression vector carrying fusion suicide gene(y CDgly TK) and sh RNA against vascular endothelial growth factor(VEGF) was constructed a...AIM: To develop a potent and safe gene therapy for esophageal cancer.METHODS: An expression vector carrying fusion suicide gene(y CDgly TK) and sh RNA against vascular endothelial growth factor(VEGF) was constructed and delivered into EC9706 esophageal cancer cells by calcium phosphate nanoparticles(CPNP). To achieve tumor selectivity, expression of the fusion suicide gene was driven by a tumor-specific human telomerase reverse transcriptase(h TERT) promoter. The biologic properties and therapeutic efficiency of the vector, in the presence of prodrug 5-fluorocytosine(5-FC), were evaluated in vitro and in vivo.RESULTS: Both in vitro and in vivo testing showed that the expression vector was efficiently introduced by CPNP into tumor cells, leading to cellular expression of y CDgly TK and decreased VEGF level. With exposure to 5-FC, it exhibited strong anti-tumor effects against esophageal cancer. Combination of VEGF sh RNA with the fusion suicide gene demonstrated strong anti-tumor activity.CONCLUSION: The sh VEGF-h TERT-y CDgly TK/5-FC system provided a novel approach for esophageal cancer-targeted gene therapy.展开更多
Self-excited oscillation in a collapsible tube is an important phenomenon in physiology. An experimental approach on self-excited oscillation in a thin-walled collapsible tube is developed by using a high transmittanc...Self-excited oscillation in a collapsible tube is an important phenomenon in physiology. An experimental approach on self-excited oscillation in a thin-walled collapsible tube is developed by using a high transmittance and low Young's modulus silicone rubber tube. The elastic tube is manufactured by the method of centrifugal casting in our laboratory. An optical method for recording the evolution of the cross-sectional areas at a certain position along the longitudinal direction of the tube is developed based on the technology of refractive index matching. With the transparent tube, the tube law is measured under the static no-flow condition. The cross section at the middle position of the tube transfers from a quasi-circular configuration to an ellipse, and then to a dumbell-shape as the chamber pressure is increased. During the self-excited oscillation, two periodic self-excited oscillating states and one transitional oscillating state are identified.They all belong to the LU mode. These different oscillating states are related to the initial cross-sectional shape of the tube caused by the difference of the downstream transmural pressure.展开更多
基金Supported by The National Natural Science Foundation of China,No. 30070235,No. 30470508 and No. 30870695The Natural Science Foundation of Hunan Province,No. 06JJ2008,07JJ6040
文摘AIM:To investigate apparent diffusion coefficient (ADC) values as an indication of reconditioning of acute hepatic injury (AHI) after allogeneic mononuclear bone marrow cell (MBMC) transplantation.METHODS:Three groups were used in our study:a cell transplantation group (n=21),transplantation control group (n=21) and normal control group (n=10).AHI model rabbits in the cell transplantation group were injected with 5 mL of MBMC suspension at multiple sites in the liver and the transplantation controls were injected with 5 mL D-Hanks solution.At the end of the 1st,2nd and 4th wk,7 rabbits were randomly selected from the cell transplantation group and transplantation control group for magnetic resonance diffusion-weighted imaging (MR-DWI) and measurement of the mean ADC values of injured livers.After MR-DWI examination,the rabbits were sacrificed and the livers subjected to pathological examination.Ten healthy rabbits from the normal control group were used for MRDWI examination and measurement of the mean ADC value of normal liver.RESULTS:At all time points,the liver pathological scores from the cell transplantation group were significantly lower than those in the transplantation control group (27.14±1.46 vs 69.29±6.16,22.29±2.29 vs 57.00±1.53,19.00±2.31vs 51.86±6.04,P=0.000).The mean ADC values of the cell transplantation group were significantly higher than the transplantation control group ((1.07±0.07)×10-3 mm2/svs (0.69±0.05)×10-3 mm2/s,(1.41±0.04)×10-3 mm2/s vs (0.84±0.06)×10-3 mm2/s,(1.68±0.04)×10-3 mm2/svs (0.86±0.04)×10-3 mm2/s,P=0.000).The pathological scores of the cell transplantation group and transplantation control group gradually decreased.However,their mean ADC values gradually increased to near that of the normal control.At the end of the 1st wk,the mean ADC values of the cell transplantation group and transplantation control group were significantly lower than those of the normal control group [(1.07±0.07)×10-3 mm2/s vs (1.76±0.03)×10-3 mm2/s,(0.69±0.05)×10-3 mm2/s vs (1.76±0.03)×10-3 mm2/s,P=0.000].At any 2 time points,the pathological scores and the mean ADC values of the cell transplantation group were significantly different (P=0.000).At the end of the 1st wk,the pathological scores and the mean ADC values of the transplantation control group were significantly different from those at the end of the 2nd and 4th wk (P=0.000).However,there was no significant difference between the 2nd and 4th wk (P=0.073 and 0.473,respectively).The coefficient of correlation between the pathological score and the mean ADC value in the cell transplantation group was-0.883 (P=0.000) and-0.762 (P=0.000) in the transplantation control group.CONCLUSION:Tracking the longitudinally dynamic change in the mean ADC value of the AHI liver may reflect hepatic injury reconditioning after allogeneic MBMC transplantation.
基金Supported by National Natural Science Foundation of ChinaNo.81372904+3 种基金No.81272971No.81272735 and No.30800518Science and Technology Department of Hunan ProvinceNo.2010CK3013
文摘AIM: To develop a potent and safe gene therapy for esophageal cancer.METHODS: An expression vector carrying fusion suicide gene(y CDgly TK) and sh RNA against vascular endothelial growth factor(VEGF) was constructed and delivered into EC9706 esophageal cancer cells by calcium phosphate nanoparticles(CPNP). To achieve tumor selectivity, expression of the fusion suicide gene was driven by a tumor-specific human telomerase reverse transcriptase(h TERT) promoter. The biologic properties and therapeutic efficiency of the vector, in the presence of prodrug 5-fluorocytosine(5-FC), were evaluated in vitro and in vivo.RESULTS: Both in vitro and in vivo testing showed that the expression vector was efficiently introduced by CPNP into tumor cells, leading to cellular expression of y CDgly TK and decreased VEGF level. With exposure to 5-FC, it exhibited strong anti-tumor effects against esophageal cancer. Combination of VEGF sh RNA with the fusion suicide gene demonstrated strong anti-tumor activity.CONCLUSION: The sh VEGF-h TERT-y CDgly TK/5-FC system provided a novel approach for esophageal cancer-targeted gene therapy.
基金support from the National Nature Science Foundation of China (Grants 11372305 and 11002138)K.C. Wong Education Foundation for a Royal Society K.C. Wong Postdoctoral Fellowship
文摘Self-excited oscillation in a collapsible tube is an important phenomenon in physiology. An experimental approach on self-excited oscillation in a thin-walled collapsible tube is developed by using a high transmittance and low Young's modulus silicone rubber tube. The elastic tube is manufactured by the method of centrifugal casting in our laboratory. An optical method for recording the evolution of the cross-sectional areas at a certain position along the longitudinal direction of the tube is developed based on the technology of refractive index matching. With the transparent tube, the tube law is measured under the static no-flow condition. The cross section at the middle position of the tube transfers from a quasi-circular configuration to an ellipse, and then to a dumbell-shape as the chamber pressure is increased. During the self-excited oscillation, two periodic self-excited oscillating states and one transitional oscillating state are identified.They all belong to the LU mode. These different oscillating states are related to the initial cross-sectional shape of the tube caused by the difference of the downstream transmural pressure.
