The grass spikelet is a unique inflorescence structure that determines grain size.Although many genetic factors have been well characterized for grain size and glume development,the underlying molecular mechanisms in ...The grass spikelet is a unique inflorescence structure that determines grain size.Although many genetic factors have been well characterized for grain size and glume development,the underlying molecular mechanisms in rice are far from established.Here,we isolated rice gene,AGL1 that controlled grain size and determines the fate of the sterile lemma.Loss of function of AGL1 produced larger grains and reduced the size of the sterile lemma.Larger grains in the agl1 mutant were caused by a larger number of cells that were longer and wider than in the wild type.The sterile lemma in the mutant spikelet was converted to a rudimentary glume-like organ.Our findings showed that the AGL1(also named LAX1)protein positively regulated G1 expression,and negatively regulated NSG1 expression,thereby affecting the fate of the sterile lemma.Taken together,our results revealed that AGL1 played a key role in negative regulation of grain size by controlling cell proliferation and expansion,and supported the opinion that rudimentary glume and sterile lemma in rice are homologous organs.展开更多
The continuous eddy current pulse treatment(ECPT)combined with heat treatment was employed to heal the microcracks in spin formed Mg alloy tubes and improve their mechanical properties in this study.The results show t...The continuous eddy current pulse treatment(ECPT)combined with heat treatment was employed to heal the microcracks in spin formed Mg alloy tubes and improve their mechanical properties in this study.The results show that all the microcracks in different tube specimens were almost healed after different continuous ECPT schemes up to 15 cycles.The schemes with less cooling intervals exhibited better healing effect and increased the strength and elongation of Mg alloy tubes more obviously.After aging treatment,the strength improvement of the specimens with ECPT was more remarkable than that of the specimens without ECPT,and the elongation decrease of the specimens with ECPT was less evident than that of specimens without ECPT due to the segregation of RE elements on the crack surface.Besides,after solution treatment,the strength reduction and ductility improvement of the specimens with ECPT were more pronounced than that of the specimens without ECPT owing to the notable decrease of dislocation density of the specimens with ECPT.Both narrowed cracks induced by ECPT and the segregation of precipitates in the vicinity of microcrack surface during aging treatment contributed to the maximum strength in the as-spun specimens with ECPT followed by aging treatment.展开更多
Grain size is an important determinant of grain yield in rice.Although dozens of grain size genes have been reported,the molecular mechanisms that control grain size remain to be fully clarified.Here,we report the clo...Grain size is an important determinant of grain yield in rice.Although dozens of grain size genes have been reported,the molecular mechanisms that control grain size remain to be fully clarified.Here,we report the cloning and characterization of GR5(GRAIN ROUND 5),which is allelic to SMOS1/SHB/RLA1/NGR5 and en-codes an AP2 transcription factor.GR5 acts as a transcriptional activator and determines grain size by influencing cell proliferation and expansion.We demonstrated that GR5 physically interacts withfive Gg subunit proteins(RGG1,RGG2,DEP1,GS3,and GGC2)and acts downstream of the G protein complex.Four downstream target genes of GR5 in grain development(DEP2,DEP3,DRW1,and CyCD5;2)were re-vealed and their core T/CGCAC motif identified by yeast one-hybrid,EMSA,and ChIP–PCR experiments.Our results revealed that GR5 interacts with Gg subunits and cooperatively determines grain size by regu-lating the expression of downstream target genes.Thesefindings provide new insight into the genetic reg-ulatory network of the G protein signaling pathway in the control of grain size and provide a potential target for high-yield rice breeding.展开更多
Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, rnulti-tillering dwarf1 （mtdl）, a stable multi-tiller and dwarf mutant, was sc...Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, rnulti-tillering dwarf1 （mtdl）, a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtdl mutant exhibited pleiotropic phenotypes, including dwarf- ism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOCOsO9g02650 （BC12） in mtdl mutant had a single nucleotide substitution （G to A）, which gen- erated a premature translation stop. Over-expressing MTD1/BC12 coding sequ（nce rescued all the phenotypes of mtdl mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtdl mutant. Quantitative reverse tran,-eription-PCR analysis showed that MTDI/BCI2 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remark- ably, a-amylase activity analysis and gibberellic acid （GA） treatment showed that the dwarf phenotype of mtdl mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.展开更多
Grain size determines grain weight and affects grain quality. Several major quantitative trait loci （QTLs） regulating grain size have been cloned; however, our understanding of the underlying mechanism that regulate...Grain size determines grain weight and affects grain quality. Several major quantitative trait loci （QTLs） regulating grain size have been cloned; however, our understanding of the underlying mechanism that regulates the size of rice grains remains fragmentary. Here, we report the cloning and characterization of a dominant QTL, GRAIN SIZE ON CHROMOSOME 2 （GS2）, which encodes Growth-Regulating Factor 4 （OsGRF4）, a transcriptional regulator. GS2 localizes to the nucleus and may act as a transcription activator. A rare mutation of GS2 affecting the binding site of a microRNA, OsmiR396c, causes elevated expression of GS2/OsGRF4. The increase in GS2 expression leads to larger cells and increased numbers of cells, which thus enhances grain weight and yield. The introduction of this rare allele of GS2/OsGRF4 into rice cultivars could significantly enhance grain weight and increase grain yield, with possible applications in breeding high-yield rice varieties.展开更多
This paper presents a novel tracking algorithm which integrates two complementary trackers. Firstly, an improved Bayesian tracker(B-tracker) with adaptive learning rate is presented. The classification score of B-trac...This paper presents a novel tracking algorithm which integrates two complementary trackers. Firstly, an improved Bayesian tracker(B-tracker) with adaptive learning rate is presented. The classification score of B-tracker reflects tracking reliability, and a low score usually results from large appearance change. Therefore, if the score is low, we decrease the learning rate to update the classifier fast so that B-tracker can adapt to the variation and vice versa. In this way, B-tracker is more suitable than its traditional version to solve appearance change problem. Secondly, we present an improved incremental subspace learning method tracker(Stracker). We propose to calculate projected coordinates using maximum posterior probability, which results in a more accurate reconstruction error than traditional subspace learning tracker. Instead of updating at every time, we present a stopstrategy to deal with occlusion problem. Finally, we present an integrated framework(BAST), in which the pair of trackers run in parallel and return two candidate target states separately. For each candidate state, we define a tracking reliability metrics to measure whether the candidate state is reliable or not, and the reliable candidate state will be chosen as the target state at the end of each frame. Experimental results on challenging sequences show that the proposed approach is very robust and effective in comparison to the state-of-the-art trackers.展开更多
Rice(Oryza sativa)spikelets are a unique inflorescence structure and their development directly determines grain size and yield.Although many genes related to spikelet development have been reported,the molecular mech...Rice(Oryza sativa)spikelets are a unique inflorescence structure and their development directly determines grain size and yield.Although many genes related to spikelet development have been reported,the molecular mechanisms underlying this process have not been fully elucidated.In this study,we identified a new recessive rice mutant,lacking rudimentary glume 1(lrg1).The lrg1 spikelets only formed one rudimentary glume,which,along with the sterile lemmas,was homeotically transformed into lemma-like organs and acquired lemma identity.The transition from the spikelet to the floral meristem was delayed in the lrg1 mutant,resulting in the formation of an ectopic lemma-like organ between the sterile lemma and the terminal floret.In addition,we found that the abnormal lrg1 grain phenotype resulted from the alteration of cell numbers and the hull size.LRG1 encodes a ZOS4-06-C2H2 zinc-finger protein with the typical EAR motifs,and is expressed in all organs and tissues.LRG1 localizes to the nucleus and can interact with the TOPLESS-RELATED PROTEINs(TPRs)to repress the expressions of their downstream target genes.Taken together,our results reveal that LRG1 plays an important role in the regulation of spikelet organ identity and grain size.展开更多
The aerial parts of higher plants are generated from the shoot apical meristem （SAM）. In this study, we isolated a small rice （Oryza sativa L.） mutant that showed premature termination of shoot development and was...The aerial parts of higher plants are generated from the shoot apical meristem （SAM）. In this study, we isolated a small rice （Oryza sativa L.） mutant that showed premature termination of shoot development and was named mini rice 1 （mini1）. The mutant was first isolated from a japonica cultivar Zhonghua11 （ZH11） subjected to ethyl methanesulfonate （EMS） treatment. With bulked segregant analysis （BSA） and mapbased cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6 kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp. The candidate gene Os09go363900 was predicted to encode a putative adhesion of calyx edges protein ACE （putative HOTHEAD precursor） and genetic complementation experiment confirmed the identity of Minil. Os09go36：3900 contains glucose-methanol-choline （GMC） oxidoreductase and NAD（P）-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD （HTH）. Expression analysis indicated Minil was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant. We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice.展开更多
In recent years,there are numerous works been proposed to leverage the techniques of deep learning to improve social-aware recommendation performance.In most cases,it requires a larger number of data to train a robust...In recent years,there are numerous works been proposed to leverage the techniques of deep learning to improve social-aware recommendation performance.In most cases,it requires a larger number of data to train a robust deep learning model,which contains a lot of parameters to fit training data.However,both data of user ratings and social networks are facing critical sparse problem,which makes it not easy to train a robust deep neural network model.Towards this problem,we propose a novel correlative denoising autoencoder(CoDAE)method by taking correlations between users with multiple roles into account to learn robust representations from sparse inputs of ratings and social networks for recommendation.We develop the CoDAE model by utilizing three separated autoencoders to learn user features with roles of rater,truster and trustee,respectively.Especially,on account of that each input unit of user vectors with roles of truster and trustee is corresponding to a particular user,we propose to utilize shared parameters to learn common information of the units that corresponding to same users.Moreover,we propose a related regularization term to learn correlations between user features that learnt by the three subnetworks of CoDAE model.We further conduct a series of experiments to evaluate the proposed method on two public datasets for Top-N recommendation task.The experimental results demonstrate that the proposed model outperforms state-of-the-art algorithms on rank-sensitive metrics of MAP and NDCG.展开更多
Moderate plant height and successful establishment of reproductive organs play pivotal roles in rice grain production. The molecular mechanism that controls the two aspects remains unclear in rice. In the present stud...Moderate plant height and successful establishment of reproductive organs play pivotal roles in rice grain production. The molecular mechanism that controls the two aspects remains unclear in rice. In the present study,we characterized a rice gene, ABNORMAL FLOWER AND DWARF1（AFD1） that determined plant height, floral development and grain yield. The afd1 mutant showed variable defects including the dwarfism, long panicle, low seed setting and reduced grain yield. In addition, abnormal floral organs were also observed in the afd1 mutant including slender and thick hulls, and hull-like lodicules.AFD1 encoded a DUF640 domain protein and was expressed in all tested tissues and organs. Subcellular localization showed AFD1-green fluorescent fusion protein（GFP） was localized in the nucleus. Meantime, our results suggested that AFD1 regulated the expression of cell division and expansion related genes.展开更多
Rice （Oryza sativa L.） is an important crop providing staple food for more than half the world＇s population and also considered as a model plant for molecular biological study of the cereals. In 1998, the large-sca...Rice （Oryza sativa L.） is an important crop providing staple food for more than half the world＇s population and also considered as a model plant for molecular biological study of the cereals. In 1998, the large-scale sequencing of japonica rice cultivar Nipponbare （bred at Aichi Agricultural Center in Japan and released in 1963） was initiated by International Rice Genome Sequencing Project （IRGSP） and the high-quality draft of genome was announced in 2002 （Goff et al,, 2002）. Owing to its easy genetic transformation and released whole genome sequencing data, Nipponbare is widely used in functional genomic research （Piffanelli et al., 2007; Luan et al., 2008; Hu et al., 2010; Thang et al., 2010; Tabuchi et al., 2011; Zhang et al., 2011; Jiang et al., 2012; Lu et al.. 2013）.展开更多
基金supported by the National Natural Science Foundation of China(32372118,32188102,32071993)the Qian Qian Academician Workstation,Specific Research Fund of the Innovation Platform for Academicians in Hainan Province(YSPTZX202303)+1 种基金Key Research and Development Program of Zhejiang Province(2021C02056)Hainan Seed Industry Laboratory,China(B21HJ0220)。
文摘The grass spikelet is a unique inflorescence structure that determines grain size.Although many genetic factors have been well characterized for grain size and glume development,the underlying molecular mechanisms in rice are far from established.Here,we isolated rice gene,AGL1 that controlled grain size and determines the fate of the sterile lemma.Loss of function of AGL1 produced larger grains and reduced the size of the sterile lemma.Larger grains in the agl1 mutant were caused by a larger number of cells that were longer and wider than in the wild type.The sterile lemma in the mutant spikelet was converted to a rudimentary glume-like organ.Our findings showed that the AGL1(also named LAX1)protein positively regulated G1 expression,and negatively regulated NSG1 expression,thereby affecting the fate of the sterile lemma.Taken together,our results revealed that AGL1 played a key role in negative regulation of grain size by controlling cell proliferation and expansion,and supported the opinion that rudimentary glume and sterile lemma in rice are homologous organs.
基金the National Natural Science Foundation of China(Nos.51775137 and 51635005)。
文摘The continuous eddy current pulse treatment(ECPT)combined with heat treatment was employed to heal the microcracks in spin formed Mg alloy tubes and improve their mechanical properties in this study.The results show that all the microcracks in different tube specimens were almost healed after different continuous ECPT schemes up to 15 cycles.The schemes with less cooling intervals exhibited better healing effect and increased the strength and elongation of Mg alloy tubes more obviously.After aging treatment,the strength improvement of the specimens with ECPT was more remarkable than that of the specimens without ECPT,and the elongation decrease of the specimens with ECPT was less evident than that of specimens without ECPT due to the segregation of RE elements on the crack surface.Besides,after solution treatment,the strength reduction and ductility improvement of the specimens with ECPT were more pronounced than that of the specimens without ECPT owing to the notable decrease of dislocation density of the specimens with ECPT.Both narrowed cracks induced by ECPT and the segregation of precipitates in the vicinity of microcrack surface during aging treatment contributed to the maximum strength in the as-spun specimens with ECPT followed by aging treatment.
基金supported by the National Key Research and Development Program of China (2022YFF1002901)the Hainan Yazhou Bay Seed Laboratory (B21HJ0215)+4 种基金the Natural Science Foundation of China (32272109)the Nanfan special project (ZDXM2315)of CAASthe Natural Science Foundation of China (32072048,U2004204)the Qian Qian Academician Workstation,the specific research fund of the Innovation Platform for Academicians of Hainan Province (YSPTZX202303)the Key Research and Development Program of Zhejiang Province (2021C02056).
文摘Grain size is an important determinant of grain yield in rice.Although dozens of grain size genes have been reported,the molecular mechanisms that control grain size remain to be fully clarified.Here,we report the cloning and characterization of GR5(GRAIN ROUND 5),which is allelic to SMOS1/SHB/RLA1/NGR5 and en-codes an AP2 transcription factor.GR5 acts as a transcriptional activator and determines grain size by influencing cell proliferation and expansion.We demonstrated that GR5 physically interacts withfive Gg subunit proteins(RGG1,RGG2,DEP1,GS3,and GGC2)and acts downstream of the G protein complex.Four downstream target genes of GR5 in grain development(DEP2,DEP3,DRW1,and CyCD5;2)were re-vealed and their core T/CGCAC motif identified by yeast one-hybrid,EMSA,and ChIP–PCR experiments.Our results revealed that GR5 interacts with Gg subunits and cooperatively determines grain size by regu-lating the expression of downstream target genes.Thesefindings provide new insight into the genetic reg-ulatory network of the G protein signaling pathway in the control of grain size and provide a potential target for high-yield rice breeding.
基金supported by the National Natural Science Foundation of China (31401464, 31201183)Zhejiang Provincial Natural Science Foundation of China (Y3110194, LY16C130001)+1 种基金China Postdoctoral Science Foundation (2014M561108)the Open Foundation from Zhejiang Provincial Top Key Discipline of Biology (KFJJ2014006)
文摘Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, rnulti-tillering dwarf1 （mtdl）, a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtdl mutant exhibited pleiotropic phenotypes, including dwarf- ism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOCOsO9g02650 （BC12） in mtdl mutant had a single nucleotide substitution （G to A）, which gen- erated a premature translation stop. Over-expressing MTD1/BC12 coding sequ（nce rescued all the phenotypes of mtdl mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtdl mutant. Quantitative reverse tran,-eription-PCR analysis showed that MTDI/BCI2 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remark- ably, a-amylase activity analysis and gibberellic acid （GA） treatment showed that the dwarf phenotype of mtdl mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.
文摘Grain size determines grain weight and affects grain quality. Several major quantitative trait loci （QTLs） regulating grain size have been cloned; however, our understanding of the underlying mechanism that regulates the size of rice grains remains fragmentary. Here, we report the cloning and characterization of a dominant QTL, GRAIN SIZE ON CHROMOSOME 2 （GS2）, which encodes Growth-Regulating Factor 4 （OsGRF4）, a transcriptional regulator. GS2 localizes to the nucleus and may act as a transcription activator. A rare mutation of GS2 affecting the binding site of a microRNA, OsmiR396c, causes elevated expression of GS2/OsGRF4. The increase in GS2 expression leads to larger cells and increased numbers of cells, which thus enhances grain weight and yield. The introduction of this rare allele of GS2/OsGRF4 into rice cultivars could significantly enhance grain weight and increase grain yield, with possible applications in breeding high-yield rice varieties.
基金This paper was supported by the National Natural Science Foundation of China (Grant No. 61472289)the National Key Research and Development Project of China (2016YFC0106305).
文摘This paper presents a novel tracking algorithm which integrates two complementary trackers. Firstly, an improved Bayesian tracker(B-tracker) with adaptive learning rate is presented. The classification score of B-tracker reflects tracking reliability, and a low score usually results from large appearance change. Therefore, if the score is low, we decrease the learning rate to update the classifier fast so that B-tracker can adapt to the variation and vice versa. In this way, B-tracker is more suitable than its traditional version to solve appearance change problem. Secondly, we present an improved incremental subspace learning method tracker(Stracker). We propose to calculate projected coordinates using maximum posterior probability, which results in a more accurate reconstruction error than traditional subspace learning tracker. Instead of updating at every time, we present a stopstrategy to deal with occlusion problem. Finally, we present an integrated framework(BAST), in which the pair of trackers run in parallel and return two candidate target states separately. For each candidate state, we define a tracking reliability metrics to measure whether the candidate state is reliable or not, and the reliable candidate state will be chosen as the target state at the end of each frame. Experimental results on challenging sequences show that the proposed approach is very robust and effective in comparison to the state-of-the-art trackers.
基金supported by the Zhejiang Natural Science Foundation(LY18C130007)the National Natural Science Foundation of China(91735304)+1 种基金the Central Public-interest Scientific Institution Basal Research Fund of China National Rice Research Institute(2017RG001-4)the National Science and Technology Major Project(2016ZX08009003-003-008)。
文摘Rice(Oryza sativa)spikelets are a unique inflorescence structure and their development directly determines grain size and yield.Although many genes related to spikelet development have been reported,the molecular mechanisms underlying this process have not been fully elucidated.In this study,we identified a new recessive rice mutant,lacking rudimentary glume 1(lrg1).The lrg1 spikelets only formed one rudimentary glume,which,along with the sterile lemmas,was homeotically transformed into lemma-like organs and acquired lemma identity.The transition from the spikelet to the floral meristem was delayed in the lrg1 mutant,resulting in the formation of an ectopic lemma-like organ between the sterile lemma and the terminal floret.In addition,we found that the abnormal lrg1 grain phenotype resulted from the alteration of cell numbers and the hull size.LRG1 encodes a ZOS4-06-C2H2 zinc-finger protein with the typical EAR motifs,and is expressed in all organs and tissues.LRG1 localizes to the nucleus and can interact with the TOPLESS-RELATED PROTEINs(TPRs)to repress the expressions of their downstream target genes.Taken together,our results reveal that LRG1 plays an important role in the regulation of spikelet organ identity and grain size.
基金supported by grants from the National Natural Science Foundation of China (No. 31201194)
文摘The aerial parts of higher plants are generated from the shoot apical meristem （SAM）. In this study, we isolated a small rice （Oryza sativa L.） mutant that showed premature termination of shoot development and was named mini rice 1 （mini1）. The mutant was first isolated from a japonica cultivar Zhonghua11 （ZH11） subjected to ethyl methanesulfonate （EMS） treatment. With bulked segregant analysis （BSA） and mapbased cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6 kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp. The candidate gene Os09go363900 was predicted to encode a putative adhesion of calyx edges protein ACE （putative HOTHEAD precursor） and genetic complementation experiment confirmed the identity of Minil. Os09go36：3900 contains glucose-methanol-choline （GMC） oxidoreductase and NAD（P）-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD （HTH）. Expression analysis indicated Minil was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant. We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice.
基金supported by the National Natural Science Foundation of China(Grant No.61472289)the National Key Research and Development Project(2016YFC0106305).
文摘In recent years,there are numerous works been proposed to leverage the techniques of deep learning to improve social-aware recommendation performance.In most cases,it requires a larger number of data to train a robust deep learning model,which contains a lot of parameters to fit training data.However,both data of user ratings and social networks are facing critical sparse problem,which makes it not easy to train a robust deep neural network model.Towards this problem,we propose a novel correlative denoising autoencoder(CoDAE)method by taking correlations between users with multiple roles into account to learn robust representations from sparse inputs of ratings and social networks for recommendation.We develop the CoDAE model by utilizing three separated autoencoders to learn user features with roles of rater,truster and trustee,respectively.Especially,on account of that each input unit of user vectors with roles of truster and trustee is corresponding to a particular user,we propose to utilize shared parameters to learn common information of the units that corresponding to same users.Moreover,we propose a related regularization term to learn correlations between user features that learnt by the three subnetworks of CoDAE model.We further conduct a series of experiments to evaluate the proposed method on two public datasets for Top-N recommendation task.The experimental results demonstrate that the proposed model outperforms state-of-the-art algorithms on rank-sensitive metrics of MAP and NDCG.
基金supported by the National Natural Science Foundation of China (31401464 and 91435105416)supported by the Open Foundation from Top Key Discipline of Modern agricultural Biotechnology and Biological Control of Crop Diseases in Zhejiang Provincial Collegethe Agricultural Sciences and Technologies Innovation Program of Chinese Academy of Agricultural Sciences (CAAS) to Rice Reproductive Developmental Biology Group
文摘Moderate plant height and successful establishment of reproductive organs play pivotal roles in rice grain production. The molecular mechanism that controls the two aspects remains unclear in rice. In the present study,we characterized a rice gene, ABNORMAL FLOWER AND DWARF1（AFD1） that determined plant height, floral development and grain yield. The afd1 mutant showed variable defects including the dwarfism, long panicle, low seed setting and reduced grain yield. In addition, abnormal floral organs were also observed in the afd1 mutant including slender and thick hulls, and hull-like lodicules.AFD1 encoded a DUF640 domain protein and was expressed in all tested tissues and organs. Subcellular localization showed AFD1-green fluorescent fusion protein（GFP） was localized in the nucleus. Meantime, our results suggested that AFD1 regulated the expression of cell division and expansion related genes.
基金supported by grants from the National Natural Science Foundation of China (Nos.31201194 and 31221004)
文摘Rice （Oryza sativa L.） is an important crop providing staple food for more than half the world＇s population and also considered as a model plant for molecular biological study of the cereals. In 1998, the large-scale sequencing of japonica rice cultivar Nipponbare （bred at Aichi Agricultural Center in Japan and released in 1963） was initiated by International Rice Genome Sequencing Project （IRGSP） and the high-quality draft of genome was announced in 2002 （Goff et al,, 2002）. Owing to its easy genetic transformation and released whole genome sequencing data, Nipponbare is widely used in functional genomic research （Piffanelli et al., 2007; Luan et al., 2008; Hu et al., 2010; Thang et al., 2010; Tabuchi et al., 2011; Zhang et al., 2011; Jiang et al., 2012; Lu et al.. 2013）.
