[Objectives]To establish the quality standard of Nardostachys jatamansi Herba.[Methods]The characters and microscopical identification of N.jatamansi Herba were carried out.The contents of moisture,total ash,acid-inso...[Objectives]To establish the quality standard of Nardostachys jatamansi Herba.[Methods]The characters and microscopical identification of N.jatamansi Herba were carried out.The contents of moisture,total ash,acid-insoluble ash and extract were determined according to the relevant methods of the Chinese Pharmacopoeia(2020 edition).Using chlorogenic acid and 3,5-O-dicaffeoylquinic acid as quality control indexes,TLC and HPLC methods were established for qualitative and quantitative determination,and HPLC fingerprints were established.[Results]The characteristics of character identification,microscopic identification and thin layer identification were obvious.The moisture content ranged from 2.7%to 7.8%,with an average value of 5.4%.The total ash content ranged from 6.7%to 16.2%,with an average of 11.0%.The acid-insoluble ash content ranged from 0.7%to 8.5%,with an average of 3.6%.Extractives content ranged from 20.9%to 34.4%,with an average of 29.7%.Chlorogenic acid content was between 0.45%and 1.30%,with an average value of 0.77%.The content of 3,5-O-dicaffeoylquinic acid ranged from 0.18%to 0.58%,with an average of 0.31%.The similarity of each batch was between 0.930 and 0.994,indicating that the quality of medicinal materials from different producing areas was stable.[Conclusions]The quality standard of N.jatamansi Herba was established,which could provide quality control basis for rational,comprehensive and efficient utilization of N.jatamansi DC.resources and clinical use.展开更多
[Objectives]To investigate the acute toxicity and hepatoprotective effect of Jinchuan formula plum wine extract on mice,determine its safety range,and evaluate its hepatoprotective effect.[Methods]The median lethal do...[Objectives]To investigate the acute toxicity and hepatoprotective effect of Jinchuan formula plum wine extract on mice,determine its safety range,and evaluate its hepatoprotective effect.[Methods]The median lethal dose(LD_(50))was determined by acute toxicity test with the toxic reaction and mortality of mice as indexes.Sixty Kunming mice were randomly divided into 6 groups:normal control group,model group(ConA-induced liver injury model),Jinchuan formula plum wine high,medium and low dose groups(1.0,0.5,0.25 g/kg)and silybin group(0.1 g/kg).The levels of ALT,AST,LDH in serum and TG,VLDL in liver were measured.After HE staining,the pathological changes of liver tissue in mice were observed,and the liver protective effect of Jinchuan formula plum wine extract was analyzed and evaluated.[Results]LD_(50)was 11.18 g/kg,and the 95%confidence limit of LD_(50)was 10.31-12.05 g/kg.The high-dose group of Jinchuan formula plum wine extract could significantly reduce the serum ALT and AST activities of ConA-induced liver injury mice(P<0.05).[Conclusions]Jinchuan formula plum wine extract is relatively safe,and also has a protective effect on liver injury.展开更多
[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823 cells were treated with high,medium and low concentratio...[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823 cells were treated with high,medium and low concentrations of drug-containing serum(0.316%,0.158%and 0.079%)for 0,48,72 and 96 h,respectively.Then,the proliferation of the cells was detected with CCK-8 method,and the expression of related proteins,B lymphocytoma-2(Bcl-2),phosphorylated protein kinase B(p-Akt),protein kinase B(Akt)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH),was detected using Western blotting.[Results]The proliferation of the BGC-823 cells was significantly inhibited with different doses of Boenninghausenia albiflora(Hook.)Reichb.ex Meisn.var.albiflora(CH)and B.sessilicarpa Lévl.(S)(P<0.01),in dose-dependent and time-dependent manners.The inhibition of high-dose S on cell proliferation was similar to that of CTX 48 h after administration;the inhibition of high-dose CH on cell proliferation was significantly stronger than that of CTX(P<0.01);different doses of drug administration groups significantly inhibited the expression of p-Akt and Bcl-2 in the BGC-823 cells;the inhibition of high-dose CH on the expression of P-Akt and Bcl-2 and the inhibition of medium-dose CH on the expression of Bcl-2 were significantly stronger than that of CTX(P<0.05,P<0.01),in a certain dose-dependent manner;at the same dose,the inhibition of CH on the expression of the proteins was stronger than that of S(P<0.05,P<0.01);administration of S and CH significantly inhibited the expression of GAPDH compared with CTX(P<0.05,P<0.01).[Conclusions]Obazema has the capacity to inhibit the proliferation of BGC-823 cells.The mechanism may be achieved by inhibiting the expression of p-Akt and Bcl-2,and GAPDH may be the target gene of its anti-tumor mechanism.The inhibiting effect of CH on BGC-823 cells was more significantly than that of S.展开更多
[Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in roo...[Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in root,stem and leaf from healthy and diseased plants. Infrared characteristic absorption peaks of healthy and diseased plants were found by infrared fingerprint method for comparative analysis.[Results]Total saponins content in root sample J4 of healthy plant reached 4. 89% and was the highest,while it was 0. 13% in stem sample J11 of healthy plant and was the lowest. Total saponins content in root sample B1 of diseased plant was 1. 68% and was the highest,while it was 0. 1% in stem sample B7 of diseased plant and was the lowest. In healthy and diseased plants,total saponins content in root was significantly higher than that in stem and leaf. Content of total saponins in root from healthy plant was significantly higher than that from diseased plant,and there was little difference in stem and leaf of healthy and diseased plants. Root sample Jg of healthy plant had obvious C-O characteristic vibration of polysaccharides and glycosides and absorption peak of O-H bending vibration in steroidal saponin near 1 160. 02 cm-1,while diseased plant had no obvious absorption peak. Near 861. 97 and 768. 07 cm-1,root sample Jg of healthy plant had significant C-C stretching vibration in sugar ring and characteristic absorption peak of characteristic vibration of steroidal saponin,while root sample Bg of diseased plant had no obvious characteristic peak. [Conclusions] Diseases significantly affected composition and content of total saponins and steroidal saponins from P. polyphylla var. chinensis,further affecting quality of P. polyphylla var. chinensis.展开更多
[Objectives] To analyze the similarities of Boenninghausenia sessilicarpa Levl. and Boenninghausenia albiflora(Hook.) Meiss by the common and variant peak ratio dual indicator sequence method. [Methods] Four different...[Objectives] To analyze the similarities of Boenninghausenia sessilicarpa Levl. and Boenninghausenia albiflora(Hook.) Meiss by the common and variant peak ratio dual indicator sequence method. [Methods] Four different solvents(petroleum ether, chloroform, ethyl acetate and methanol) were used to extract the chemical components of different polar regions of B. sessilicarpa and B. albiflora. UV-visible spectrophotometry(second derivative method) was used to collect the fingerprints of different solvent extracts, and common and variant peak peak ratios were analyzed for the absorption peak data. [Results] The common peak ratio and variant peak ratio data of ground parts of B. albiflora and B. sessilicarpa was S2∶S5(46.2(54.2, 62.5)), compared with the data of other groups, the common peak ratio was the largest, thus the components of ground parts of B. albiflora and B. sessilicarpa were closest and had the largest similarities; the common peak ratio and variant peak ratio data of the components extracted by chloroform from B. albiflora and B. sessilicarpa was Y2∶Y6(54.2(38.5, 46.2)), compared with the data of other groups, the variant peak ratio was the smallest, thus, the chemical components near the chloroform polarity of two kinds of medicinal materials had the largest similarities and smallest differences. [Conclusions] This method is simple and easy to operate, and the ultraviolet fingerprint data of four different polar organic solvent extracts are used for comprehensive analysis, and the results have high specificity and high accuracy. Besides, there are certain similarities and also differences between the chemical components of B. sessilicarpa and B. albiflora. It is expected to provide a new evaluation method for the variety quality of B. sessilicarpa and B. albiflora.展开更多
[Objectives] To determine the content of 20 inorganic elements in 18 samples of roots, stems and leaves from of Arctium lappa L. produced in different areas. [Methods] The wet digestion and technique of inductively co...[Objectives] To determine the content of 20 inorganic elements in 18 samples of roots, stems and leaves from of Arctium lappa L. produced in different areas. [Methods] The wet digestion and technique of inductively coupled plasma emission spectrometry(ICP-OES) were used. [Results] 20 kinds of inorganic elements in the samples of A. lappa L. roots contained rich elements essential for human beings such as K, Ca, Na, P and trace elements Cu, Fe, Zn; heavy metals Pb, As, Cu, Cd in A. lappa L. samples did not exceed the limit, and Hg was not detected in all 12 samples; heavy metals in A. lappa L. roots harvested in 12 months did not exceed the limit. [Conclusions] Compared with the roots of A. lappa L., the inorganic elements in the stems and leaves of A. lappa L. were relatively less. The samples collected from Shuimo Town in Wenchuan County of Aba Prefecture with an altitude of 920 m showed little difference from samples collected from Qiongxi Town in Hongyuan County of Aba Prefecture with an altitude of 3 505 m in 20 kinds of inorganic elements.展开更多
[Objectives] This study aimed to provide experimental data for the identification and application of Leontopodium franchetii Beauv. [Methods] The original plants, traits, microstructure and powder of L. franchetii Bea...[Objectives] This study aimed to provide experimental data for the identification and application of Leontopodium franchetii Beauv. [Methods] The original plants, traits, microstructure and powder of L. franchetii Beauv. were identified. [Results] The identification characteristics of L. franchetii Beauv. in the original plants, traits and microstructure were obvious. [Conclusions] This study can provide reference for the identification of original plants and herbs and the development of quality standards of L. franchetii Beauv. and provide a basis for further research and development.展开更多
基金Supported by National Key R&D Plan Project (2018YFC1708005)Application Foundation Project of Sichuan Provincial Department of Science and Technology (20YYJC3299)Special Fund for Basic Scientific Research Business Expenses of Central Universities of Southwest Minzu University (2020NGD01).
文摘[Objectives]To establish the quality standard of Nardostachys jatamansi Herba.[Methods]The characters and microscopical identification of N.jatamansi Herba were carried out.The contents of moisture,total ash,acid-insoluble ash and extract were determined according to the relevant methods of the Chinese Pharmacopoeia(2020 edition).Using chlorogenic acid and 3,5-O-dicaffeoylquinic acid as quality control indexes,TLC and HPLC methods were established for qualitative and quantitative determination,and HPLC fingerprints were established.[Results]The characteristics of character identification,microscopic identification and thin layer identification were obvious.The moisture content ranged from 2.7%to 7.8%,with an average value of 5.4%.The total ash content ranged from 6.7%to 16.2%,with an average of 11.0%.The acid-insoluble ash content ranged from 0.7%to 8.5%,with an average of 3.6%.Extractives content ranged from 20.9%to 34.4%,with an average of 29.7%.Chlorogenic acid content was between 0.45%and 1.30%,with an average value of 0.77%.The content of 3,5-O-dicaffeoylquinic acid ranged from 0.18%to 0.58%,with an average of 0.31%.The similarity of each batch was between 0.930 and 0.994,indicating that the quality of medicinal materials from different producing areas was stable.[Conclusions]The quality standard of N.jatamansi Herba was established,which could provide quality control basis for rational,comprehensive and efficient utilization of N.jatamansi DC.resources and clinical use.
文摘[Objectives]To investigate the acute toxicity and hepatoprotective effect of Jinchuan formula plum wine extract on mice,determine its safety range,and evaluate its hepatoprotective effect.[Methods]The median lethal dose(LD_(50))was determined by acute toxicity test with the toxic reaction and mortality of mice as indexes.Sixty Kunming mice were randomly divided into 6 groups:normal control group,model group(ConA-induced liver injury model),Jinchuan formula plum wine high,medium and low dose groups(1.0,0.5,0.25 g/kg)and silybin group(0.1 g/kg).The levels of ALT,AST,LDH in serum and TG,VLDL in liver were measured.After HE staining,the pathological changes of liver tissue in mice were observed,and the liver protective effect of Jinchuan formula plum wine extract was analyzed and evaluated.[Results]LD_(50)was 11.18 g/kg,and the 95%confidence limit of LD_(50)was 10.31-12.05 g/kg.The high-dose group of Jinchuan formula plum wine extract could significantly reduce the serum ALT and AST activities of ConA-induced liver injury mice(P<0.05).[Conclusions]Jinchuan formula plum wine extract is relatively safe,and also has a protective effect on liver injury.
基金Supported by National Key Technology Research and Development Program(2018FYC1708000)Basic Research Project for Application of Science and Technology in Sichuan Province(2017JY0274)Science and Technology Research Project of Sichuan Traditional Chinese Medicine Administration(2018JC028).
文摘[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823 cells were treated with high,medium and low concentrations of drug-containing serum(0.316%,0.158%and 0.079%)for 0,48,72 and 96 h,respectively.Then,the proliferation of the cells was detected with CCK-8 method,and the expression of related proteins,B lymphocytoma-2(Bcl-2),phosphorylated protein kinase B(p-Akt),protein kinase B(Akt)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH),was detected using Western blotting.[Results]The proliferation of the BGC-823 cells was significantly inhibited with different doses of Boenninghausenia albiflora(Hook.)Reichb.ex Meisn.var.albiflora(CH)and B.sessilicarpa Lévl.(S)(P<0.01),in dose-dependent and time-dependent manners.The inhibition of high-dose S on cell proliferation was similar to that of CTX 48 h after administration;the inhibition of high-dose CH on cell proliferation was significantly stronger than that of CTX(P<0.01);different doses of drug administration groups significantly inhibited the expression of p-Akt and Bcl-2 in the BGC-823 cells;the inhibition of high-dose CH on the expression of P-Akt and Bcl-2 and the inhibition of medium-dose CH on the expression of Bcl-2 were significantly stronger than that of CTX(P<0.05,P<0.01),in a certain dose-dependent manner;at the same dose,the inhibition of CH on the expression of the proteins was stronger than that of S(P<0.05,P<0.01);administration of S and CH significantly inhibited the expression of GAPDH compared with CTX(P<0.05,P<0.01).[Conclusions]Obazema has the capacity to inhibit the proliferation of BGC-823 cells.The mechanism may be achieved by inhibiting the expression of p-Akt and Bcl-2,and GAPDH may be the target gene of its anti-tumor mechanism.The inhibiting effect of CH on BGC-823 cells was more significantly than that of S.
基金Supported by the National Key R&D Program(2018YFC1708005)the Sichuan Science and Technology Plan Project(2018JY0111)the Innovating Research Program of Postgraduates of Southwest Minzu University in 2018(cx2018sz83)
文摘[Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in root,stem and leaf from healthy and diseased plants. Infrared characteristic absorption peaks of healthy and diseased plants were found by infrared fingerprint method for comparative analysis.[Results]Total saponins content in root sample J4 of healthy plant reached 4. 89% and was the highest,while it was 0. 13% in stem sample J11 of healthy plant and was the lowest. Total saponins content in root sample B1 of diseased plant was 1. 68% and was the highest,while it was 0. 1% in stem sample B7 of diseased plant and was the lowest. In healthy and diseased plants,total saponins content in root was significantly higher than that in stem and leaf. Content of total saponins in root from healthy plant was significantly higher than that from diseased plant,and there was little difference in stem and leaf of healthy and diseased plants. Root sample Jg of healthy plant had obvious C-O characteristic vibration of polysaccharides and glycosides and absorption peak of O-H bending vibration in steroidal saponin near 1 160. 02 cm-1,while diseased plant had no obvious absorption peak. Near 861. 97 and 768. 07 cm-1,root sample Jg of healthy plant had significant C-C stretching vibration in sugar ring and characteristic absorption peak of characteristic vibration of steroidal saponin,while root sample Bg of diseased plant had no obvious characteristic peak. [Conclusions] Diseases significantly affected composition and content of total saponins and steroidal saponins from P. polyphylla var. chinensis,further affecting quality of P. polyphylla var. chinensis.
基金Supported by State Key Research and Development Program of Ministry of Science and Technology(2018YFC1708000)Scientific and Technological Project at Department and Bureau Level(2018JC028)Fundamental Research Funds for the Central Universities of Southwest Minzu University(2018NQN13)
文摘[Objectives] To analyze the similarities of Boenninghausenia sessilicarpa Levl. and Boenninghausenia albiflora(Hook.) Meiss by the common and variant peak ratio dual indicator sequence method. [Methods] Four different solvents(petroleum ether, chloroform, ethyl acetate and methanol) were used to extract the chemical components of different polar regions of B. sessilicarpa and B. albiflora. UV-visible spectrophotometry(second derivative method) was used to collect the fingerprints of different solvent extracts, and common and variant peak peak ratios were analyzed for the absorption peak data. [Results] The common peak ratio and variant peak ratio data of ground parts of B. albiflora and B. sessilicarpa was S2∶S5(46.2(54.2, 62.5)), compared with the data of other groups, the common peak ratio was the largest, thus the components of ground parts of B. albiflora and B. sessilicarpa were closest and had the largest similarities; the common peak ratio and variant peak ratio data of the components extracted by chloroform from B. albiflora and B. sessilicarpa was Y2∶Y6(54.2(38.5, 46.2)), compared with the data of other groups, the variant peak ratio was the smallest, thus, the chemical components near the chloroform polarity of two kinds of medicinal materials had the largest similarities and smallest differences. [Conclusions] This method is simple and easy to operate, and the ultraviolet fingerprint data of four different polar organic solvent extracts are used for comprehensive analysis, and the results have high specificity and high accuracy. Besides, there are certain similarities and also differences between the chemical components of B. sessilicarpa and B. albiflora. It is expected to provide a new evaluation method for the variety quality of B. sessilicarpa and B. albiflora.
基金Supported by The National Key Research and Development Program of China(2018YFC1708005)Science and Technology Planning Project of Sichuan Province(2016NZYZF0007)+1 种基金Fourth National Survey of Traditional Chinese Medicine Resources Program(2017)Innovating Research Program of Postgraduates of Southwest University for Nationalities in 2019(CX2019SZ175)
文摘[Objectives] To determine the content of 20 inorganic elements in 18 samples of roots, stems and leaves from of Arctium lappa L. produced in different areas. [Methods] The wet digestion and technique of inductively coupled plasma emission spectrometry(ICP-OES) were used. [Results] 20 kinds of inorganic elements in the samples of A. lappa L. roots contained rich elements essential for human beings such as K, Ca, Na, P and trace elements Cu, Fe, Zn; heavy metals Pb, As, Cu, Cd in A. lappa L. samples did not exceed the limit, and Hg was not detected in all 12 samples; heavy metals in A. lappa L. roots harvested in 12 months did not exceed the limit. [Conclusions] Compared with the roots of A. lappa L., the inorganic elements in the stems and leaves of A. lappa L. were relatively less. The samples collected from Shuimo Town in Wenchuan County of Aba Prefecture with an altitude of 920 m showed little difference from samples collected from Qiongxi Town in Hongyuan County of Aba Prefecture with an altitude of 3 505 m in 20 kinds of inorganic elements.
基金Supported by National Key Research and Development Program of China(2018YFC1708005)Science and Technology Support Program of Sichuan Province(2015SZ0034)+1 种基金Fourth National Survey of Traditional Chinese Medicine Resources Program(2017)Innovating Research Program of Postgraduates of Southwest University for Nationalities in 2019(CX2019SZ173)
文摘[Objectives] This study aimed to provide experimental data for the identification and application of Leontopodium franchetii Beauv. [Methods] The original plants, traits, microstructure and powder of L. franchetii Beauv. were identified. [Results] The identification characteristics of L. franchetii Beauv. in the original plants, traits and microstructure were obvious. [Conclusions] This study can provide reference for the identification of original plants and herbs and the development of quality standards of L. franchetii Beauv. and provide a basis for further research and development.
