Objective:To explore and analyze the application value of nursing intervention for patients with pancreatitis after endoscopic retrograde cholangiopancreatography(ERCP).Methods:From May 2022 to May 2023,100 patients w...Objective:To explore and analyze the application value of nursing intervention for patients with pancreatitis after endoscopic retrograde cholangiopancreatography(ERCP).Methods:From May 2022 to May 2023,100 patients with pancreatitis after ERCP who were admitted to the General Surgery Department of our hospital were selected as the research objects,they were divided into a research group and a general group by flipping coins,with 50 cases in each group.The research group received nursing intervention,and the general group received general nursing.The postoperative index recovery time,quality of life,and emotional performance were compared between the two groups.Results:The blood amylase recovery time,abdominal pain recovery time,white blood cell recovery time,and hospitalization time in the research group were significantly lower than those in the general group(P<0.05).Before intervention,the physical function,social factors,physiological performance,emotional state,and other quality of life indicators were compared between the groups,and there was no statistically significant difference(P>0.05);after intervention,the mentioned quality of life indicators were significantly better than that of the general group(P<0.05).Before intervention,the Self-Rating Anxiety Scale(SAS)and Self-Rating Depression Scale(SDS)scores between the groups were compared,and there was no statistically significant difference(P>0.05);after intervention,the SAS and SDS scores of the research group were significantly better than those of the general group(P<0.05).Conclusion:The application of nursing intervention in patients with pancreatitis after ERCP has high clinical value and specific practical significance.展开更多
Objective:Hepatitis B virus(HBV)infection is a major public health problem worldwide.However,the regulatory mechanisms underlying HBV replication remain unclear.Cullin 4 B-RING ubiquitin E3 ligase(CRL4 B)is involved i...Objective:Hepatitis B virus(HBV)infection is a major public health problem worldwide.However,the regulatory mechanisms underlying HBV replication remain unclear.Cullin 4 B-RING ubiquitin E3 ligase(CRL4 B)is involved in regulating diverse physiological and pathophysiological processes.In our study,we aimed to explain the role of CUL4 B in HBV infection.Methods:Cul4 b transgenic mice or conditional knockout mice,as well as liver cell lines with CUL4 B overexpression or knockdown,were used to assess the role of CUL4 B in HBV replication.Immunoprecipitation assays and immunofluorescence staining were performed to study the interaction between CUL4 B and HBx.Cycloheximide chase assays and in vivo ubiquitination assays were performed to evaluate the half-life and the ubiquitination status of HBx.Results:The hydrodynamics-based hepatitis B model in Cul4 b transgenic or conditional knockout mice indicated that CUL4 B promoted HBV replication(P<0.05).Moreover,the overexpression or knockdown system in human liver cell lines validated that CUL4 B increased HBV replication in an HBx-dependent manner.Importantly,immunoprecipitation assays and immunofluorescence staining showed an interaction between CUL4 B and HBx.Furthermore,CUL4 B upregulated HBx protein levels by inhibiting HBx ubiquitination and proteasomal degradation(P<0.05).Finally,a positive correlation between CUL4 B expression and HBV pg RNA level was observed in liver tissues from HBV-positive patients and HBV transgenic mice.Conclusions:CUL4 B enhances HBV replication by interacting with HBx and disrupting its ubiquitin-dependent proteasomal degradation.CUL4 B may therefore be a potential target for anti-HBV therapy.展开更多
The responses of the upper ocean to Typhoon Haitang in July 2005 are investigated using Argo float and multiplatform satellite data.The results show decreasing sea surface temperature(SST),a deepening of the mixed lay...The responses of the upper ocean to Typhoon Haitang in July 2005 are investigated using Argo float and multiplatform satellite data.The results show decreasing sea surface temperature(SST),a deepening of the mixed layer depth(MLD),and enhanced Chlorophyll-a(Chl-a)concentration.Two extreme cool regions are identified.While the magnitude of SST cooling in the two regions is similar,the biological response(Chla enhancement)differs.To facilitate comparisons,the region to the northeast of Taiwan is defined as region A and the region east of Taiwan as region B.Ekman pumping and the intrusion of the Kuroshio play an important role in the enhancement of Chl-a in region A.Cold eddies provide the material source for the formation of the cold center in region B,where mixing is dominant.Because of the relatively high translation speed(5 m/s)in region B,Ekman pumping has little influence on the cooling and Chl-a enhancement processes.Moreover,the MLD is shallower than the nutricline,which means that mixing does not result in a marked increase in nutrients in the euphotic layer(where the nutrient concentration is uniformly depleted).Sea temperatures,in contrast,gradually decrease with depth below the bottom of the mixed layer.In contrast to region A,region B showed no significant enhancement of Chl-a but strong SST cooling.展开更多
文摘Objective:To explore and analyze the application value of nursing intervention for patients with pancreatitis after endoscopic retrograde cholangiopancreatography(ERCP).Methods:From May 2022 to May 2023,100 patients with pancreatitis after ERCP who were admitted to the General Surgery Department of our hospital were selected as the research objects,they were divided into a research group and a general group by flipping coins,with 50 cases in each group.The research group received nursing intervention,and the general group received general nursing.The postoperative index recovery time,quality of life,and emotional performance were compared between the two groups.Results:The blood amylase recovery time,abdominal pain recovery time,white blood cell recovery time,and hospitalization time in the research group were significantly lower than those in the general group(P<0.05).Before intervention,the physical function,social factors,physiological performance,emotional state,and other quality of life indicators were compared between the groups,and there was no statistically significant difference(P>0.05);after intervention,the mentioned quality of life indicators were significantly better than that of the general group(P<0.05).Before intervention,the Self-Rating Anxiety Scale(SAS)and Self-Rating Depression Scale(SDS)scores between the groups were compared,and there was no statistically significant difference(P>0.05);after intervention,the SAS and SDS scores of the research group were significantly better than those of the general group(P<0.05).Conclusion:The application of nursing intervention in patients with pancreatitis after ERCP has high clinical value and specific practical significance.
基金supported by grants from the National Natural Science Foundation of China(Grant Nos.81970508,81672425,818300178,8197148,81670520,and 31671427)the National Key Research and Development Program(Grant No.2018YFE0126500)+1 种基金the Key Research&Development Plan of Shandong Province(Grant No.2018YFJH0503)support from the Collaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong。
文摘Objective:Hepatitis B virus(HBV)infection is a major public health problem worldwide.However,the regulatory mechanisms underlying HBV replication remain unclear.Cullin 4 B-RING ubiquitin E3 ligase(CRL4 B)is involved in regulating diverse physiological and pathophysiological processes.In our study,we aimed to explain the role of CUL4 B in HBV infection.Methods:Cul4 b transgenic mice or conditional knockout mice,as well as liver cell lines with CUL4 B overexpression or knockdown,were used to assess the role of CUL4 B in HBV replication.Immunoprecipitation assays and immunofluorescence staining were performed to study the interaction between CUL4 B and HBx.Cycloheximide chase assays and in vivo ubiquitination assays were performed to evaluate the half-life and the ubiquitination status of HBx.Results:The hydrodynamics-based hepatitis B model in Cul4 b transgenic or conditional knockout mice indicated that CUL4 B promoted HBV replication(P<0.05).Moreover,the overexpression or knockdown system in human liver cell lines validated that CUL4 B increased HBV replication in an HBx-dependent manner.Importantly,immunoprecipitation assays and immunofluorescence staining showed an interaction between CUL4 B and HBx.Furthermore,CUL4 B upregulated HBx protein levels by inhibiting HBx ubiquitination and proteasomal degradation(P<0.05).Finally,a positive correlation between CUL4 B expression and HBV pg RNA level was observed in liver tissues from HBV-positive patients and HBV transgenic mice.Conclusions:CUL4 B enhances HBV replication by interacting with HBx and disrupting its ubiquitin-dependent proteasomal degradation.CUL4 B may therefore be a potential target for anti-HBV therapy.
基金supported by the National Natural Science Foundation of China(40976011)the National Basic Research Program of China(2013CB430300)the Public Science and Technology Research Funds Projects of Ocean(201105018)
文摘The responses of the upper ocean to Typhoon Haitang in July 2005 are investigated using Argo float and multiplatform satellite data.The results show decreasing sea surface temperature(SST),a deepening of the mixed layer depth(MLD),and enhanced Chlorophyll-a(Chl-a)concentration.Two extreme cool regions are identified.While the magnitude of SST cooling in the two regions is similar,the biological response(Chla enhancement)differs.To facilitate comparisons,the region to the northeast of Taiwan is defined as region A and the region east of Taiwan as region B.Ekman pumping and the intrusion of the Kuroshio play an important role in the enhancement of Chl-a in region A.Cold eddies provide the material source for the formation of the cold center in region B,where mixing is dominant.Because of the relatively high translation speed(5 m/s)in region B,Ekman pumping has little influence on the cooling and Chl-a enhancement processes.Moreover,the MLD is shallower than the nutricline,which means that mixing does not result in a marked increase in nutrients in the euphotic layer(where the nutrient concentration is uniformly depleted).Sea temperatures,in contrast,gradually decrease with depth below the bottom of the mixed layer.In contrast to region A,region B showed no significant enhancement of Chl-a but strong SST cooling.
