AIM:To establish the presence of the hypothalamic hormone protirelin(thyrotropin-releasing hormone,TRH)in human thyroid and to investigate whether the concentration of this peptide in the thyroid gland is sensitive to...AIM:To establish the presence of the hypothalamic hormone protirelin(thyrotropin-releasing hormone,TRH)in human thyroid and to investigate whether the concentration of this peptide in the thyroid gland is sensitive to thyroid status.METHODS:A procedure has been developed for the determination of TRH in the thyroid gland,distinct from TRH-like peptides which also react with TRH-antibody.RESULTS:Human thyroid was shown to contain both authentic TRH and TRH-like peptides,a similar pattern was seen in a range of animal thyroids.The concentrations of TRH in non-active goiter thyroids were substantial(41.6-248 pmol·g-1);in contrast the thyroids from hyperthyroid patients contained very little TRH(0.01-2.52 pmol·g-1).CONCLUSION:The physiologic role of TRH in the thyroid is not known but the large difference between the concentrations of this hormone in non-active and hyperactive thyroids suggests that thyroidal TRH may be involved in the regulation of thyroid status.展开更多
基金This work was suported in part by EC Science Plan SCI-CT92-0762 to DGS and JRGby a grant(00206/CV/97)from the Seneca Foundation,Murcia,Spain.
文摘AIM:To establish the presence of the hypothalamic hormone protirelin(thyrotropin-releasing hormone,TRH)in human thyroid and to investigate whether the concentration of this peptide in the thyroid gland is sensitive to thyroid status.METHODS:A procedure has been developed for the determination of TRH in the thyroid gland,distinct from TRH-like peptides which also react with TRH-antibody.RESULTS:Human thyroid was shown to contain both authentic TRH and TRH-like peptides,a similar pattern was seen in a range of animal thyroids.The concentrations of TRH in non-active goiter thyroids were substantial(41.6-248 pmol·g-1);in contrast the thyroids from hyperthyroid patients contained very little TRH(0.01-2.52 pmol·g-1).CONCLUSION:The physiologic role of TRH in the thyroid is not known but the large difference between the concentrations of this hormone in non-active and hyperactive thyroids suggests that thyroidal TRH may be involved in the regulation of thyroid status.
