This study was conducted to identify bacterial strain HeNan-001 isolated from intestinal tract of healthy rex rabbit. The strain was identified by gram staining, and OD600 values at different culture time were measure...This study was conducted to identify bacterial strain HeNan-001 isolated from intestinal tract of healthy rex rabbit. The strain was identified by gram staining, and OD600 values at different culture time were measured to develop a bacterial growth curve. The metabolic pathways of the bacterium using sugar in fermentation was identified with biochemical tubes. Total RNA of the strain was extracted, and total eDNA was obtained by Oligo （dT） method. Primers were designed using Primer 5.0 hip-software according to the 16S rRNA sequence published in GenBank, through cloning, ligation to vector PMD18T, construction of PMD18T/16S rRNA vector and transformation into DHSα, plasmid was extracted and sequenced, and the sequencing result was compared with sequences in NCBI followed by drawing of phylogenetic tree. The strain was verified to be double-stranded gram-positive cocci, which could ferment glucose, mannose, maltose and sorbitol, producing acids production, but failed to ferment sucrose, serum inulin and β-galactosidase. The phylogenetie tree analysis showed that the isolated bacterium shared the highest homology with an India isolate, and belongs to diplostreptococcus. This study provides significance experimental data and theoretical guidance for further development and utilization of the bacterial strain.展开更多
Investigations on adsorption behavior of triphenylene(TP) and subsequent graphene self-assembly on Cu(111) were carried out mainly by using scanning tunneling microscopy(STM).At monolayer coverage,TP molecules formed ...Investigations on adsorption behavior of triphenylene(TP) and subsequent graphene self-assembly on Cu(111) were carried out mainly by using scanning tunneling microscopy(STM).At monolayer coverage,TP molecules formed a longrange ordered adsorption structure on Cu(111) with an uniform orientation.Graphene self-assembly on the Cu(111) substrate with TP molecules as precursor was achieved by annealing the sample,and a large-scale graphene overlayer was successfully captured after the sample annealing up to 1000 K.Three different Moire patterns generated from relative rotational disorders between the graphene overlayer and the Cu(111) substrate were observed,one with 40 rotation between the graphene overlayer and the Cu(111) substrate with a periodicity of 2.93 nm,another with 70 rotation and 2.15 nm of the size of the Moire supercell,and the third with 100 rotation with a periodicity of 1.35 nm.展开更多
Bioorthogonal reactions involving transition metals have diversified applications in imaging,drug development,chemical catalysis and other fields.Transition metals used to catalyze the bioorthogonal reaction mainly in...Bioorthogonal reactions involving transition metals have diversified applications in imaging,drug development,chemical catalysis and other fields.Transition metals used to catalyze the bioorthogonal reaction mainly include ruthenium,palladium,copper,and gold.However,the great potential for translational applications of bioorthogonal reaction needs to be further expanded and their reaction efficiency should be improved.Therefore,it is an urgent need for the development of this field to find more suitable catalysts to efficiently catalyze existing biological orthogonal reactions and expand the types of biological orthogonal reactions.Thus,this review not only summarizes those transition metal complexes-based catalysts participating in bioorthogonal reaction and some bioorthogonal reactions involving transition metals inside the cells,but also sheds light into the discovery of new transition metal complexes and their future development in applications.展开更多
Herein,a novel strategy was exploited to achieve the delamination of TiCMXene multilayers into ultrathin flakes by blossom of ZnInSmicroflowers via a one-pot solvothermal method.There is no need to peel off the MXene ...Herein,a novel strategy was exploited to achieve the delamination of TiCMXene multilayers into ultrathin flakes by blossom of ZnInSmicroflowers via a one-pot solvothermal method.There is no need to peel off the MXene bulk ahead of its combination with the semiconductor.The obtained ZnInS/TiCbinary composites were applied for visible-light-driven photocatalytic hydrogen production without noble metal cocatalyst,and the optimized sample exhibited a hydrogen-production efficiency of 978.7 μmol hgwith the corresponding apparent quantum efficiency of 24.2% at 420 nm,which was 2.7 times higher than bare ZnInS.Through the comprehensive analysis based on spectroscopy measurements,electrochemical techniques and energy band theory,such enhancement was mainly attributed to(1) the highly-exposed surface that was beneficial for the adequate exposure of reactive sites and(2)the intimate contact interface that favored the transfer of photogenerated carriers.This study provides a new way of thinking for synthesizing ultrathin MXene-based composite materials for noble-metal-free and highly-efficient photocatalysis applications.展开更多
Hydrogels have blossomed as superstars in various fields,owing to their prospective applications in tissue engineering,soft electronics and sensors,flexible energy storage,and biomedicines.Two-dimensional(2D)nanomater...Hydrogels have blossomed as superstars in various fields,owing to their prospective applications in tissue engineering,soft electronics and sensors,flexible energy storage,and biomedicines.Two-dimensional(2D)nanomaterials,especially 2D mono-elemental nanosheets(Xenes)exhibit high aspect ratio morphology,good biocompatibility,metallic conductivity,and tunable electrochemical properties.These fascinating characteristics endow numerous tunable application-specific properties for the construction of Xene-based hydrogels.Hierarchical multifunctional hydrogels can be prepared according to the application requirements and can be effectively tuned by different stimulation to complete specific tasks in a spatiotemporal sequence.In this review,the synthesis mechanism,properties,and emerging applications of Xene hydrogels are summarized,followed by a discussion on expanding the performance and application range of both hydrogels and Xenes.展开更多
A lumped mass finite element formulation with consistent nodal quadrature is presented for improved frequency analysis of wave equations with particular reference to the Lagrangian elements with Lobatto nodes.For the ...A lumped mass finite element formulation with consistent nodal quadrature is presented for improved frequency analysis of wave equations with particular reference to the Lagrangian elements with Lobatto nodes.For the Lagrangian Lobatto elements,a lumped mass matrix can be conveniently constructed by employing the nodal quadrature rule that takes the Lobatto nodes as integration points.In the conventional finite element analysis,this nodal quadrature-based lumped mass matrix is usually accompanied by the stiffness matrix computed via the Gauss quadrature.In this work,it is shown that this combination is not optimal regarding the frequency accuracy of finite element analysis of wave equations.To elevate the frequency accuracy,in addition to the lumped mass matrix formulated by the nodal quadrature,a frequency accuracy measure is established as a function of the quadrature rule used in the stiffness matrix integration.This accuracy measure discloses that the frequency accuracy can be optimized if both lumped mass and stiffness matrices are simultaneously computed by the same nodal quadrature rule.These theoretical results are well demonstrated by two-and three-dimensional numerical examples,which clearly show that the proposed consistent nodal quadrature formulation yields much higher frequency accuracy than the conventional finite element analysis with nodal quadrature-based lumped mass and Gauss quadrature-based stiffness matrices for wave equations.展开更多
基金Supported by Modern Agricultural Technology System Construction Project of Shandong Province(SDAIT-21-15)Shen Zhiqiang Innovation Team Program of Animal Science and Veterinary Service of Shandong Province(LFFW[2014]01041)
文摘This study was conducted to identify bacterial strain HeNan-001 isolated from intestinal tract of healthy rex rabbit. The strain was identified by gram staining, and OD600 values at different culture time were measured to develop a bacterial growth curve. The metabolic pathways of the bacterium using sugar in fermentation was identified with biochemical tubes. Total RNA of the strain was extracted, and total eDNA was obtained by Oligo （dT） method. Primers were designed using Primer 5.0 hip-software according to the 16S rRNA sequence published in GenBank, through cloning, ligation to vector PMD18T, construction of PMD18T/16S rRNA vector and transformation into DHSα, plasmid was extracted and sequenced, and the sequencing result was compared with sequences in NCBI followed by drawing of phylogenetic tree. The strain was verified to be double-stranded gram-positive cocci, which could ferment glucose, mannose, maltose and sorbitol, producing acids production, but failed to ferment sucrose, serum inulin and β-galactosidase. The phylogenetie tree analysis showed that the isolated bacterium shared the highest homology with an India isolate, and belongs to diplostreptococcus. This study provides significance experimental data and theoretical guidance for further development and utilization of the bacterial strain.
基金Project supported by the National Key Research and Development Program of China(Grant No.2017YFB0503100)the National Natural Science Foundation of China(Grant No.11790313).
文摘Investigations on adsorption behavior of triphenylene(TP) and subsequent graphene self-assembly on Cu(111) were carried out mainly by using scanning tunneling microscopy(STM).At monolayer coverage,TP molecules formed a longrange ordered adsorption structure on Cu(111) with an uniform orientation.Graphene self-assembly on the Cu(111) substrate with TP molecules as precursor was achieved by annealing the sample,and a large-scale graphene overlayer was successfully captured after the sample annealing up to 1000 K.Three different Moire patterns generated from relative rotational disorders between the graphene overlayer and the Cu(111) substrate were observed,one with 40 rotation between the graphene overlayer and the Cu(111) substrate with a periodicity of 2.93 nm,another with 70 rotation and 2.15 nm of the size of the Moire supercell,and the third with 100 rotation with a periodicity of 1.35 nm.
基金supported by the National Natural Science Foundation for Distinguished Young Scholars (82225025)the National Natural Science Foundation of China (21877049,22177038,32171296)+1 种基金Guangdong Natural Science Foundation (2020B1515120043,2022A1515012235)K.C.Wong Education Foundation。
文摘Bioorthogonal reactions involving transition metals have diversified applications in imaging,drug development,chemical catalysis and other fields.Transition metals used to catalyze the bioorthogonal reaction mainly include ruthenium,palladium,copper,and gold.However,the great potential for translational applications of bioorthogonal reaction needs to be further expanded and their reaction efficiency should be improved.Therefore,it is an urgent need for the development of this field to find more suitable catalysts to efficiently catalyze existing biological orthogonal reactions and expand the types of biological orthogonal reactions.Thus,this review not only summarizes those transition metal complexes-based catalysts participating in bioorthogonal reaction and some bioorthogonal reactions involving transition metals inside the cells,but also sheds light into the discovery of new transition metal complexes and their future development in applications.
基金supported by the National Natural Science Foundation of China (21972171)the Hubei Provincial Natural Science Foundation, China (2021CFA022)National Innovation and Entrepreneurship Training Program for College Students, SouthCentral Minzu University (202110524009)。
文摘Herein,a novel strategy was exploited to achieve the delamination of TiCMXene multilayers into ultrathin flakes by blossom of ZnInSmicroflowers via a one-pot solvothermal method.There is no need to peel off the MXene bulk ahead of its combination with the semiconductor.The obtained ZnInS/TiCbinary composites were applied for visible-light-driven photocatalytic hydrogen production without noble metal cocatalyst,and the optimized sample exhibited a hydrogen-production efficiency of 978.7 μmol hgwith the corresponding apparent quantum efficiency of 24.2% at 420 nm,which was 2.7 times higher than bare ZnInS.Through the comprehensive analysis based on spectroscopy measurements,electrochemical techniques and energy band theory,such enhancement was mainly attributed to(1) the highly-exposed surface that was beneficial for the adequate exposure of reactive sites and(2)the intimate contact interface that favored the transfer of photogenerated carriers.This study provides a new way of thinking for synthesizing ultrathin MXene-based composite materials for noble-metal-free and highly-efficient photocatalysis applications.
基金supported by a grant from the National Natural Science Foundation of China(Grant No.32071322to X.J.),National Natural Science Foundation of China for Excellent Young Scholar(Grant No.32122044+8 种基金to X.J.),and the Technology&Innovation Commission of Shenzhen Municipality(Grant No.JCYJ20210324113004010to X.J.).W.T.acknowledges the supports from American Heart Association Collaborative Science Award(No.2018A004190to W.T.),Harvard Medical School/Brigham and Women’s Hospital Department of Anesthesiology-Basic Scientist Grant(No.2420 BPA075toW.T.),Nanotechnology Foundation(No.2022A002721to W.T.),Gillian Reny Stepping Strong Center for Trauma Innovation Breakthrough Innovator Award(No.113548to W.T.),Center for Nanomedicine Research Fund(No.2019A014810to W.T.),Khoury Innovation Award(No.2020A003219to W.T.),and Farokhzad Family Distinguished Chair Foundation(No.018129to W.T.)。
文摘Hydrogels have blossomed as superstars in various fields,owing to their prospective applications in tissue engineering,soft electronics and sensors,flexible energy storage,and biomedicines.Two-dimensional(2D)nanomaterials,especially 2D mono-elemental nanosheets(Xenes)exhibit high aspect ratio morphology,good biocompatibility,metallic conductivity,and tunable electrochemical properties.These fascinating characteristics endow numerous tunable application-specific properties for the construction of Xene-based hydrogels.Hierarchical multifunctional hydrogels can be prepared according to the application requirements and can be effectively tuned by different stimulation to complete specific tasks in a spatiotemporal sequence.In this review,the synthesis mechanism,properties,and emerging applications of Xene hydrogels are summarized,followed by a discussion on expanding the performance and application range of both hydrogels and Xenes.
基金supported by the National Natural Science Foundation of China(Grant Nos.12072302 and 11772280)the Natural Science Foundation of Fujian Province of China(Grant No.2021J02003)the Natural Science Foundation of Hebei Province of China(Grant No.A2018209319).
文摘A lumped mass finite element formulation with consistent nodal quadrature is presented for improved frequency analysis of wave equations with particular reference to the Lagrangian elements with Lobatto nodes.For the Lagrangian Lobatto elements,a lumped mass matrix can be conveniently constructed by employing the nodal quadrature rule that takes the Lobatto nodes as integration points.In the conventional finite element analysis,this nodal quadrature-based lumped mass matrix is usually accompanied by the stiffness matrix computed via the Gauss quadrature.In this work,it is shown that this combination is not optimal regarding the frequency accuracy of finite element analysis of wave equations.To elevate the frequency accuracy,in addition to the lumped mass matrix formulated by the nodal quadrature,a frequency accuracy measure is established as a function of the quadrature rule used in the stiffness matrix integration.This accuracy measure discloses that the frequency accuracy can be optimized if both lumped mass and stiffness matrices are simultaneously computed by the same nodal quadrature rule.These theoretical results are well demonstrated by two-and three-dimensional numerical examples,which clearly show that the proposed consistent nodal quadrature formulation yields much higher frequency accuracy than the conventional finite element analysis with nodal quadrature-based lumped mass and Gauss quadrature-based stiffness matrices for wave equations.
