Mobile Industrial Internet of Things(IIoT)applications have achieved the explosive growth in recent years.The mobile IIoT has flourished and become the backbone of the industry,laying a solid foundation for the interc...Mobile Industrial Internet of Things(IIoT)applications have achieved the explosive growth in recent years.The mobile IIoT has flourished and become the backbone of the industry,laying a solid foundation for the interconnection of all things.The variety of application scenarios has brought serious challenges to mobile IIoT networks,which face complex and changeable communication environments.Ensuring data secure transmission is critical for mobile IIoT networks.This paper investigates the data secure transmission performance prediction of mobile IIoT networks.To cut down computational complexity,we propose a data secure transmission scheme employing Transmit Antenna Selection(TAS).The novel secrecy performance expressions are first derived.Then,to realize real-time secrecy analysis,we design an improved Convolutional Neural Network(CNN)model,and propose an intelligent data secure transmission performance prediction algorithm.For mobile signals,the important features may be removed by the pooling layers.This will lead to negative effects on the secrecy performance prediction.A novel nine-layer improved CNN model is designed.Out of the input and output layers,it removes the pooling layer and contains six convolution layers.Elman,Back-Propagation(BP)and LeNet methods are employed to compare with the proposed algorithm.Through simulation analysis,good prediction accuracy is achieved by the CNN algorithm.The prediction accuracy obtains a 59%increase.展开更多
Intellectualization has been an inevitable trend in the information network,allowing the network to achieve the capabilities of self-learning,self-optimization,and self-evolution in the dynamic environment.Due to the ...Intellectualization has been an inevitable trend in the information network,allowing the network to achieve the capabilities of self-learning,self-optimization,and self-evolution in the dynamic environment.Due to the strong adaptability to the environment,the cognitive theory methods from psychology gradually become an excellent approach to construct the intelligent information network(IIN),making the traditional definition of the intelligent information network no longer appropriate.Moreover,the thinking capability of existing IINs is always limited.This paper redefines the intelligent information network and illustrates the required properties of the architecture,core theory,and critical technologies by analyzing the existing intelligent information network.Besides,we innovatively propose a novel network cognition model with the network knowledge to implement the intelligent information network.The proposed model can perceive the overall environment data of the network and extract the knowledge from the data.As the model’s core,the knowledge guides the model to generate the optimal decisions adapting to the environmental changes.At last,we present the critical technologies needed to accomplish the proposed network cognition model.展开更多
In recent years,as giant satellite constellations grow rapidly worldwide,the co-existence between constellations has been widely concerned.In this paper,we overview the co-frequency interference(CFI)among the giant no...In recent years,as giant satellite constellations grow rapidly worldwide,the co-existence between constellations has been widely concerned.In this paper,we overview the co-frequency interference(CFI)among the giant non-geostationary orbit(NGSO)constellations.Specifically,we first summarize the CFI scenario and evaluation index among different NGSO constellations.Based on statistics about NGSO constellation plans,we analyse the challenges in mitigation and analysis of CFI.Next,the CFI calculation methods and research progress are systematically sorted out from the aspects of interference risk analysis framework,numerical calculation and link construction.Then,the feasibility of interference mitigation technologies based on space,frequency domain isolation,power control,and interference alignment mitigation in the NGSO mega-constellation CFI scenario are further sorted out.Finally,we present promising directions for future research in CFI analysis and CFI avoidance.展开更多
Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H ...Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H vessels have recently been identified as a bone-specific vascular subtype that supports osteogenesis.Here,we show that metformin accelerated fracture healing in both osteoporotic and normal mice.Moreover,metformin promoted angiogenesis in vitro under hypoxia as well as type H vessel formation throughout fracture healing.Mechanistically,metformin increased the expression of HIF-1α,an important positive regulator of type H vessel formation,by inhibiting the expression of YAP1/TAZ in calluses and hypoxia-cultured human microvascular endothelial cells(HMECs).The results of HIF-1αor YAP1/TAZ interference in hypoxia-cultured HMECs using si RNA further suggested that the enhancement of HIF-1αand its target genes by metformin is primarily through YAP1/TAZ inhibition.Finally,overexpression of YAP1/TAZ partially counteracted the effect of metformin in promoting type H vessel-induced angiogenesis-osteogenesis coupling during fracture repair.In summary,our findings suggest that metformin has the potential to be a therapeutic agent for fractures by promoting type H vessel formation through YAP1/TAZ inhibition.展开更多
Several theoretical models have been developed so far to predict the thermal conductivities of carbon nanotube(CNT)networks.However,these models overestimated the thermal conductivity significantly.In this paper,we cl...Several theoretical models have been developed so far to predict the thermal conductivities of carbon nanotube(CNT)networks.However,these models overestimated the thermal conductivity significantly.In this paper,we claimed that a CNT network can be considered as a contact thermal resistance network.In the contact thermal resistance network,the temperature of an individual CNT is nonuniform and the intrinsic thermal resistance of CNTs can be ignored.Compared with the previous models,the model we proposed agrees well with the experimental results of single-walled CNT networks.展开更多
Pear ring rot,caused by the pathogenic fungi Botryosphaeria dothidea,seriously affects pear production.While the infection-induced reactive oxygen species(ROS)burst of infected plants limits the proliferation of B.dot...Pear ring rot,caused by the pathogenic fungi Botryosphaeria dothidea,seriously affects pear production.While the infection-induced reactive oxygen species(ROS)burst of infected plants limits the proliferation of B.dothidea during the early infection stage,high ROS levels can also contribute to their growth during the later necrotrophic infection stage.Therefore,it is important to understand how plants balance ROS levels and resistance to pathogenic B.dothidea during the later stage.In this study,we identified PbrChiA,a glycosyl hydrolases 18(GH18)chitinase-encoding gene with high infection-induced expression,through a comparative transcriptome analysis.Artificial substitution,stable overexpression,and virus induced gene silencing(VIGS)experiments demonstrated that PbrChiA can positively regulate pear resistance as a secreted chitinase to break down B.dothidea mycelium in vitro and that overexpression of PbrChiA suppressed infection-induced ROS accumulation.Further analysis revealed that PbrChiA can bind to the ectodomain of PbrLYK1b2,and this interaction suppressed PbrLYK1b2-mediated chitin-induced ROS accumulation.Collectively,we propose that the combination of higher antifungal activity from abundant PbrChiA and lower ROS levels during later necrotrophic infection stage confer resistance of pear against B.dothidea.展开更多
The current High-Speed Railway(HSR)communications increasingly fail to satisfy the massive access services of numerous user equipment brought by the increasing number of people traveling by HSRs.To this end,this paper...The current High-Speed Railway(HSR)communications increasingly fail to satisfy the massive access services of numerous user equipment brought by the increasing number of people traveling by HSRs.To this end,this paper investigates millimeter-Wave(mmWave)extra-large scale(XL)-MIMO-based massive Internet-of-Things(loT)access in near-field HSR communications,and proposes a block simultaneous orthogonal matching pursuit(B-SOMP)-based Active User Detection(AUD)and Channel Estimation(CE)scheme by exploiting the spatial block sparsity of the XLMIMO-based massive access channels.Specifically,we first model the uplink mmWave XL-MIMO channels,which exhibit the near-field propagation characteristics of electromagnetic signals and the spatial non-stationarity of mmWave XL-MIMO arrays.By exploiting the spatial block sparsity and common frequency-domain sparsity pattern of massive access channels,the joint AUD and CE problem can be then formulated as a Multiple Measurement Vectors Compressive Sensing(MIMV-CS)problem.Based on the designed sensing matrix,a B-SOMP algorithm is proposed to achieve joint AUD and CE.Finally,simulation results show that the proposed solution can obtain a better AUD and CE performance than the conventional CS-based scheme for massive IoT access in near-field HSR communications.展开更多
Recent research has shown that microRNA (miRNA), which is involved in almost every step of gastric carcinogenesis, has broad prospective application in diagnosis and therapy of gastric carcinoma. Eastern Asia (South K...Recent research has shown that microRNA (miRNA), which is involved in almost every step of gastric carcinogenesis, has broad prospective application in diagnosis and therapy of gastric carcinoma. Eastern Asia (South Korea, Japan and China) has the highest incidence of gastric cancer in the world. There were 988 000 new cases of gastric cancer worldwide and 736 000 deaths in 2008. Approximately 60% of the cases of gastric cancer are found in East Asia (mainly China). We herein provide a brief review of the clinical applications of miRNA, which include the following aspects: (1) miRNA may serve as a potential new generation of tumor markers; (2) a complete miRNA expression profile is highly specific, can reflect the evolutionary lineage and differentiation of tumors, and be used to carry out diversity analysis; (3) detecting specific miRNA expression in peripheral blood will become a new method for diagnosis of gastric cancer; (4) miRNA can predict prognosis of gastric cancer; (5) miRNA has predictive value in determining chemotherapy and radiotherapy resistance; and (6) miRNA could be a type of innovative drug. Finally, we focus on assessing the value of miRNA from laboratory to clinical application and the challenges it faces in East Asia.展开更多
Osteoporosis is a frequent complication of chronic inflammatory diseases and increases in the pro-inflammatory cytokines make an important contribution to bone loss by promoting bone resorption and impairing bone form...Osteoporosis is a frequent complication of chronic inflammatory diseases and increases in the pro-inflammatory cytokines make an important contribution to bone loss by promoting bone resorption and impairing bone formation. Omentin-1 is a newly identified adipocytokine that has anti-inflammatory effects, but little is known about the role of omentin-1 in inflammatory osteoporosis. Here we generated global omentin-1 knockout(omentin-1^(-/-)) mice and demonstrated that depletion of omentin-1 induces inflammatory bone loss-like phenotypes in mice, as defined by abnormally elevated pro-inflammatory cytokines, increased osteoclast formation and bone tissue destruction, as well as impaired osteogenic activities. Using an inflammatory cell model induced by tumor necrosis factor-α(TNF-α), we determined that recombinant omentin-1 reduces the production of proinflammatory factors in the TNF-α-activated macrophages, and suppresses their anti-osteoblastic and pro-osteoclastic abilities. In the magnesium silicate-induced inflammatory osteoporosis mouse model, the systemic administration of adenoviral-delivered omentin-1 significantly protects from osteoporotic bone loss and inflammation. Our study suggests that omentin-1 can be used as a promising therapeutic agent for the prevention or treatment of inflammatory bone diseases by downregulating the proinflammatory cytokines.展开更多
Osteoporosis is a debilitating bone disease affecting millions of people. Here, we used human urine-derived stem cells(USCs),which were noninvasively harvested from unlimited and easily available urine, as a "fac...Osteoporosis is a debilitating bone disease affecting millions of people. Here, we used human urine-derived stem cells(USCs),which were noninvasively harvested from unlimited and easily available urine, as a "factory" to obtain extracellular vesicles(USCEVs) and demonstrated that the systemic injection of USC-EVs effectively alleviates bone loss and maintains bone strength in osteoporotic mice by enhancing osteoblastic bone formation and suppressing osteoclastic bone resorption. More importantly, the anti-osteoporotic properties of USC-EVs are not notably disrupted by the age, gender, or health condition(with or without osteoporosis) of the USC donor. Mechanistic studies determined that collagen triple-helix repeat containing 1(CTHRC1) and osteoprotegerin(OPG) proteins are enriched in USC-EVs and required for USC-EV-induced pro-osteogenic and anti-osteoclastic effects. Our results suggest that autologous USC-EVs represent a promising novel therapeutic agent for osteoporosis by promoting osteogenesis and inhibiting osteoclastogenesis by transferring CTHRC1 and OPG.展开更多
In recent years,the damage caused by soil nematodes has become increasingly serious;however,the varieties and structures of the nematicides available on the market are deficient.Fluopyram,a succinate dehydrogenase inh...In recent years,the damage caused by soil nematodes has become increasingly serious;however,the varieties and structures of the nematicides available on the market are deficient.Fluopyram,a succinate dehydrogenase inhibitor(SDHI)fungicide developed by Bayer AG in Germany,has been widely used in the prevention and control of soil nematodes due to its high efficiency and novel mechanism of action.In this paper,two series of novel target compounds were designed and synthesized with nematicidal and fungicidal fluopyram as the molecular skeleton in order to introduce sulfide and sulfone substructures.The structures were identified and characterized by 1H nuclear magnetic resonance(NMR),13C NMR,and high-resolution mass spectrometer(HRMS).The bioassays revealed that most of the compounds showed excellent nematicidal activities at 200 lgmL-1 in comparison with fluopyram,while the nematode mortality rate dropped sharply at 100μg·mL-1,except for compounds I-11 and II-6.In terms of fungicidal activity,compound I-9 was discovered to have an excellent inhibitory rate,and a molecular docking simulation was performed that can provide important guidance for the design and exploration of efficient fungicidal lead compounds.展开更多
Wireless sensor networks are open architectures, so any potential threat can easily intercept, wiretap and counterfeit the information. Therefore, the safety of WSN is very important. Since any single key system canno...Wireless sensor networks are open architectures, so any potential threat can easily intercept, wiretap and counterfeit the information. Therefore, the safety of WSN is very important. Since any single key system cannot guarantee the security of the wireless sensor network for communications, this paper introduces a hierarchical key management scheme based on the different abilities of different sensor nodes in the clustered wireless sensor network. In this scheme, the nodes are distributed into several clusters, and a cluster head must be elected for each cluster. Private communication between cluster heads is realized through the encryption system based on the identity of each head while private communication between cluster nodes in a same cluster head is achieved through the random key preliminary distribution system. Considering the characteristics of WSN, we adopt dynamic means called dynamic cluster key management scheme to deal with master key, so master key will be updated according to the changed dynamic network topology. For cluster head node plays a pivotal role in this scheme, a trust manage-ment system should be introduced into the election of the cluster head which will exclude the malicious node from outside the cluster, thus improve the whole network security.展开更多
基金supported by the National Natural Science Foundation of China(No.62201313)the Opening Foundation of Fujian Key Laboratory of Sensing and Computing for Smart Cities(Xiamen University)(No.SCSCKF202101)the Open Project of Fujian Provincial Key Laboratory of Information Processing and Intelligent Control(Minjiang University)(No.MJUKF-IPIC202206).
文摘Mobile Industrial Internet of Things(IIoT)applications have achieved the explosive growth in recent years.The mobile IIoT has flourished and become the backbone of the industry,laying a solid foundation for the interconnection of all things.The variety of application scenarios has brought serious challenges to mobile IIoT networks,which face complex and changeable communication environments.Ensuring data secure transmission is critical for mobile IIoT networks.This paper investigates the data secure transmission performance prediction of mobile IIoT networks.To cut down computational complexity,we propose a data secure transmission scheme employing Transmit Antenna Selection(TAS).The novel secrecy performance expressions are first derived.Then,to realize real-time secrecy analysis,we design an improved Convolutional Neural Network(CNN)model,and propose an intelligent data secure transmission performance prediction algorithm.For mobile signals,the important features may be removed by the pooling layers.This will lead to negative effects on the secrecy performance prediction.A novel nine-layer improved CNN model is designed.Out of the input and output layers,it removes the pooling layer and contains six convolution layers.Elman,Back-Propagation(BP)and LeNet methods are employed to compare with the proposed algorithm.Through simulation analysis,good prediction accuracy is achieved by the CNN algorithm.The prediction accuracy obtains a 59%increase.
基金supported by the China Postdoctoral Science Foundation (Grant No.2020M673687)。
文摘Intellectualization has been an inevitable trend in the information network,allowing the network to achieve the capabilities of self-learning,self-optimization,and self-evolution in the dynamic environment.Due to the strong adaptability to the environment,the cognitive theory methods from psychology gradually become an excellent approach to construct the intelligent information network(IIN),making the traditional definition of the intelligent information network no longer appropriate.Moreover,the thinking capability of existing IINs is always limited.This paper redefines the intelligent information network and illustrates the required properties of the architecture,core theory,and critical technologies by analyzing the existing intelligent information network.Besides,we innovatively propose a novel network cognition model with the network knowledge to implement the intelligent information network.The proposed model can perceive the overall environment data of the network and extract the knowledge from the data.As the model’s core,the knowledge guides the model to generate the optimal decisions adapting to the environmental changes.At last,we present the critical technologies needed to accomplish the proposed network cognition model.
文摘In recent years,as giant satellite constellations grow rapidly worldwide,the co-existence between constellations has been widely concerned.In this paper,we overview the co-frequency interference(CFI)among the giant non-geostationary orbit(NGSO)constellations.Specifically,we first summarize the CFI scenario and evaluation index among different NGSO constellations.Based on statistics about NGSO constellation plans,we analyse the challenges in mitigation and analysis of CFI.Next,the CFI calculation methods and research progress are systematically sorted out from the aspects of interference risk analysis framework,numerical calculation and link construction.Then,the feasibility of interference mitigation technologies based on space,frequency domain isolation,power control,and interference alignment mitigation in the NGSO mega-constellation CFI scenario are further sorted out.Finally,we present promising directions for future research in CFI analysis and CFI avoidance.
基金supported by the National Natural Science Foundation of China (Grant Nos.81874006,82172399,81902222,82060395,81902277,82072504,82000845)the Hunan Province Natural Science Foundation of China (Grant Nos.2020JJ4928,2020JJ4897,2021JJ30038,2021JJ40492)the Independent Exploration and Innovation Project of Central South University (Grant Nos.2020zzts255)。
文摘Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H vessels have recently been identified as a bone-specific vascular subtype that supports osteogenesis.Here,we show that metformin accelerated fracture healing in both osteoporotic and normal mice.Moreover,metformin promoted angiogenesis in vitro under hypoxia as well as type H vessel formation throughout fracture healing.Mechanistically,metformin increased the expression of HIF-1α,an important positive regulator of type H vessel formation,by inhibiting the expression of YAP1/TAZ in calluses and hypoxia-cultured human microvascular endothelial cells(HMECs).The results of HIF-1αor YAP1/TAZ interference in hypoxia-cultured HMECs using si RNA further suggested that the enhancement of HIF-1αand its target genes by metformin is primarily through YAP1/TAZ inhibition.Finally,overexpression of YAP1/TAZ partially counteracted the effect of metformin in promoting type H vessel-induced angiogenesis-osteogenesis coupling during fracture repair.In summary,our findings suggest that metformin has the potential to be a therapeutic agent for fractures by promoting type H vessel formation through YAP1/TAZ inhibition.
基金Project support by the National Natural Science Foundation of China(Grant No.52127811)Department of Science and Technology of Jiangsu Province,China(Grant No.BK20220032)。
文摘Several theoretical models have been developed so far to predict the thermal conductivities of carbon nanotube(CNT)networks.However,these models overestimated the thermal conductivity significantly.In this paper,we claimed that a CNT network can be considered as a contact thermal resistance network.In the contact thermal resistance network,the temperature of an individual CNT is nonuniform and the intrinsic thermal resistance of CNTs can be ignored.Compared with the previous models,the model we proposed agrees well with the experimental results of single-walled CNT networks.
基金This work has been supported by the National Key Research and Development Program of China(2018YFD1000300)the National Science Foundation of China(31872070,32072538),Fundamental Research Funds for the Central Universities(JCQY201901)+1 种基金the Earmarked Fund for China Agriculture Research System(CARS-28)the Jiangsu Agriculture Science and Technology Innovation Fund(SCX(22)3215).Bioinformatic analysis was supported by the Bioinformatics Center of Nanjing Agricultural University.We also thank Dr Jianming Zeng(University of Macao),and all the members of his bioinformatics team,biotrainee,for generously sharing their experience and codes.
文摘Pear ring rot,caused by the pathogenic fungi Botryosphaeria dothidea,seriously affects pear production.While the infection-induced reactive oxygen species(ROS)burst of infected plants limits the proliferation of B.dothidea during the early infection stage,high ROS levels can also contribute to their growth during the later necrotrophic infection stage.Therefore,it is important to understand how plants balance ROS levels and resistance to pathogenic B.dothidea during the later stage.In this study,we identified PbrChiA,a glycosyl hydrolases 18(GH18)chitinase-encoding gene with high infection-induced expression,through a comparative transcriptome analysis.Artificial substitution,stable overexpression,and virus induced gene silencing(VIGS)experiments demonstrated that PbrChiA can positively regulate pear resistance as a secreted chitinase to break down B.dothidea mycelium in vitro and that overexpression of PbrChiA suppressed infection-induced ROS accumulation.Further analysis revealed that PbrChiA can bind to the ectodomain of PbrLYK1b2,and this interaction suppressed PbrLYK1b2-mediated chitin-induced ROS accumulation.Collectively,we propose that the combination of higher antifungal activity from abundant PbrChiA and lower ROS levels during later necrotrophic infection stage confer resistance of pear against B.dothidea.
基金supported in part by the Natural Science Foundation of China(NSFC)under Grant 62071044 and Grant 62088101in part by the Shandong Province Natural Science Foundation under Grant ZR2022YQ62in part by the Beijing Nova Program.
文摘The current High-Speed Railway(HSR)communications increasingly fail to satisfy the massive access services of numerous user equipment brought by the increasing number of people traveling by HSRs.To this end,this paper investigates millimeter-Wave(mmWave)extra-large scale(XL)-MIMO-based massive Internet-of-Things(loT)access in near-field HSR communications,and proposes a block simultaneous orthogonal matching pursuit(B-SOMP)-based Active User Detection(AUD)and Channel Estimation(CE)scheme by exploiting the spatial block sparsity of the XLMIMO-based massive access channels.Specifically,we first model the uplink mmWave XL-MIMO channels,which exhibit the near-field propagation characteristics of electromagnetic signals and the spatial non-stationarity of mmWave XL-MIMO arrays.By exploiting the spatial block sparsity and common frequency-domain sparsity pattern of massive access channels,the joint AUD and CE problem can be then formulated as a Multiple Measurement Vectors Compressive Sensing(MIMV-CS)problem.Based on the designed sensing matrix,a B-SOMP algorithm is proposed to achieve joint AUD and CE.Finally,simulation results show that the proposed solution can obtain a better AUD and CE performance than the conventional CS-based scheme for massive IoT access in near-field HSR communications.
文摘Recent research has shown that microRNA (miRNA), which is involved in almost every step of gastric carcinogenesis, has broad prospective application in diagnosis and therapy of gastric carcinoma. Eastern Asia (South Korea, Japan and China) has the highest incidence of gastric cancer in the world. There were 988 000 new cases of gastric cancer worldwide and 736 000 deaths in 2008. Approximately 60% of the cases of gastric cancer are found in East Asia (mainly China). We herein provide a brief review of the clinical applications of miRNA, which include the following aspects: (1) miRNA may serve as a potential new generation of tumor markers; (2) a complete miRNA expression profile is highly specific, can reflect the evolutionary lineage and differentiation of tumors, and be used to carry out diversity analysis; (3) detecting specific miRNA expression in peripheral blood will become a new method for diagnosis of gastric cancer; (4) miRNA can predict prognosis of gastric cancer; (5) miRNA has predictive value in determining chemotherapy and radiotherapy resistance; and (6) miRNA could be a type of innovative drug. Finally, we focus on assessing the value of miRNA from laboratory to clinical application and the challenges it faces in East Asia.
基金supported by the Excellent Young Scientist Foundation of the National Natural Science Foundation of China(Grant No.81522012)the National Natural Science Foundation of China(Grant No.81670807,81600699,81702237,81701383,81400858)+8 种基金the Thousand Youth Talents Plan of China(Grant No.D1119003)the Hunan Youth Talent Project(Grant No.2016RS3021)the Innovation Driven Project of Central South University(2016CX028)the Youth Foundation of Xiangya Hospital in Central South University(Grant No.2016Q10)the Fundamental Research Funds for the Central Universities of Central South University(Grant No.2017zzts032,2017zzts014)the Hunan Province Natural Science Foundation of China(Grant No.2017JJ3501)the China Postdoctoral Science Foundation(Grant No.2017M612596)the Natural Science Foundation for Distinguished Yong Scholars of Guangdong Province(2016A030306051)the National Basic Research Program of China(973 Program,Grant no.2014CB942903)
文摘Osteoporosis is a frequent complication of chronic inflammatory diseases and increases in the pro-inflammatory cytokines make an important contribution to bone loss by promoting bone resorption and impairing bone formation. Omentin-1 is a newly identified adipocytokine that has anti-inflammatory effects, but little is known about the role of omentin-1 in inflammatory osteoporosis. Here we generated global omentin-1 knockout(omentin-1^(-/-)) mice and demonstrated that depletion of omentin-1 induces inflammatory bone loss-like phenotypes in mice, as defined by abnormally elevated pro-inflammatory cytokines, increased osteoclast formation and bone tissue destruction, as well as impaired osteogenic activities. Using an inflammatory cell model induced by tumor necrosis factor-α(TNF-α), we determined that recombinant omentin-1 reduces the production of proinflammatory factors in the TNF-α-activated macrophages, and suppresses their anti-osteoblastic and pro-osteoclastic abilities. In the magnesium silicate-induced inflammatory osteoporosis mouse model, the systemic administration of adenoviral-delivered omentin-1 significantly protects from osteoporotic bone loss and inflammation. Our study suggests that omentin-1 can be used as a promising therapeutic agent for the prevention or treatment of inflammatory bone diseases by downregulating the proinflammatory cytokines.
基金supported by the National Natural Science Foundation of China (Grant nos. 81522012, 81702237, 81670807, 81871822, 81801395, 81600699, 81701383, and 81802138)the Thousand Youth Talents Plan of China (Grant no. D1119003)+8 种基金the Medicine and Health Science and Technology Innovation Project of Chinese Academy of Medical Sciences (Grant no. 2018-I2M-HL-024)the High Level Talent Gathering Project of Hunan Province (Grant nos. 2017XK2039, and 2018RS3029)the Innovation Driven Project of Central South University (Grant nos. 2016CX028,2019CX014, and 2018CX029)the Youth Foundation of Xiangya Hospital in Central South University (Grant no. 2016Q10)the Hunan Provincial Innovation Foundation for Postgraduate (Grant no. CX2018B045)the Fundamental Research Funds for the Central Universities of Central South University (Grant nos. 2017zzts211 and 2018zzts895)the Hunan Province Natural Science Foundation of China (Grant no. 2017JJ3501)the China Postdoctoral Science Foundation (Grant nos. 2017M612596, 2017M622614, and 2018M632998)the Natural Science Foundation for Distinguished Yong Scholars of Guangdong Province (Grant no. 2016A030306051)
文摘Osteoporosis is a debilitating bone disease affecting millions of people. Here, we used human urine-derived stem cells(USCs),which were noninvasively harvested from unlimited and easily available urine, as a "factory" to obtain extracellular vesicles(USCEVs) and demonstrated that the systemic injection of USC-EVs effectively alleviates bone loss and maintains bone strength in osteoporotic mice by enhancing osteoblastic bone formation and suppressing osteoclastic bone resorption. More importantly, the anti-osteoporotic properties of USC-EVs are not notably disrupted by the age, gender, or health condition(with or without osteoporosis) of the USC donor. Mechanistic studies determined that collagen triple-helix repeat containing 1(CTHRC1) and osteoprotegerin(OPG) proteins are enriched in USC-EVs and required for USC-EV-induced pro-osteogenic and anti-osteoclastic effects. Our results suggest that autologous USC-EVs represent a promising novel therapeutic agent for osteoporosis by promoting osteogenesis and inhibiting osteoclastogenesis by transferring CTHRC1 and OPG.
基金This work was financially supported by the Natural Science Foundation of Shandong Province,China(ZR2017BC053)the Doctoral Research Startup Foundation of Liaocheng University(318051625).
文摘In recent years,the damage caused by soil nematodes has become increasingly serious;however,the varieties and structures of the nematicides available on the market are deficient.Fluopyram,a succinate dehydrogenase inhibitor(SDHI)fungicide developed by Bayer AG in Germany,has been widely used in the prevention and control of soil nematodes due to its high efficiency and novel mechanism of action.In this paper,two series of novel target compounds were designed and synthesized with nematicidal and fungicidal fluopyram as the molecular skeleton in order to introduce sulfide and sulfone substructures.The structures were identified and characterized by 1H nuclear magnetic resonance(NMR),13C NMR,and high-resolution mass spectrometer(HRMS).The bioassays revealed that most of the compounds showed excellent nematicidal activities at 200 lgmL-1 in comparison with fluopyram,while the nematode mortality rate dropped sharply at 100μg·mL-1,except for compounds I-11 and II-6.In terms of fungicidal activity,compound I-9 was discovered to have an excellent inhibitory rate,and a molecular docking simulation was performed that can provide important guidance for the design and exploration of efficient fungicidal lead compounds.
基金supported by National Natural Science Foundation of China Grant No. 60803150, No.60803151the National High Technology Research and Development Program of China under grant Nos.2008AA01Z411+1 种基金the Key Program of NSFC-Guangdong Union Foundation under Grant No.U0835004China Postdoctoral Science Foundation No. 20090451495
文摘Wireless sensor networks are open architectures, so any potential threat can easily intercept, wiretap and counterfeit the information. Therefore, the safety of WSN is very important. Since any single key system cannot guarantee the security of the wireless sensor network for communications, this paper introduces a hierarchical key management scheme based on the different abilities of different sensor nodes in the clustered wireless sensor network. In this scheme, the nodes are distributed into several clusters, and a cluster head must be elected for each cluster. Private communication between cluster heads is realized through the encryption system based on the identity of each head while private communication between cluster nodes in a same cluster head is achieved through the random key preliminary distribution system. Considering the characteristics of WSN, we adopt dynamic means called dynamic cluster key management scheme to deal with master key, so master key will be updated according to the changed dynamic network topology. For cluster head node plays a pivotal role in this scheme, a trust manage-ment system should be introduced into the election of the cluster head which will exclude the malicious node from outside the cluster, thus improve the whole network security.
