Objective: To investigate DNA-dependent protein kinase (DNA-PK) expression,and its relationship with lymphat-ic metastasis in colorectal cancer. Methods: Tumor tissues from 60 patients,divided into two groups accordin...Objective: To investigate DNA-dependent protein kinase (DNA-PK) expression,and its relationship with lymphat-ic metastasis in colorectal cancer. Methods: Tumor tissues from 60 patients,divided into two groups according to lymphatic metastasis,were immunohistochemically stained to detect the DNA-PK expression including Ku70,Ku80 and PKcs proteins. Results: Positivity of both Ku70 and Ku80 in colorectal cancer was negatively correlated with lymphatic metastasis with an r value of -0.57 and -0.38,respectively. Similar correlation was found between Ku expression,especially Ku70,and long-term survival. PKcs,however,displayed no significant correlation. Statistical analysis failed to detect any correlation between DNA-PK expression,and clinical characteristics,such as age,sex,tumor location,tumor thickness and distant metastasis (P>0.05). Conclusion: DNA-PK expression,especially Ku70 expression,is negatively correlated with lymphatic metastasis,and the survival of patients with colorectal cancer. Ku70 expression may be a potential indicator for the preoperative evaluation,and prognosis in colorectal cancer.展开更多
As the third generation new battery,the power conversion efficiency(PCE)of metal halide perovskite solar cells(PsCs)has increased from 3.8%in 2009 to 25.8%currently certified,which fully shows that they have great res...As the third generation new battery,the power conversion efficiency(PCE)of metal halide perovskite solar cells(PsCs)has increased from 3.8%in 2009 to 25.8%currently certified,which fully shows that they have great research value and development prospect.As one of the main components of high-efficiency PSCs,hole transport materials(HTMs)play an important role in extracting and transporting holes and inhibiting charge recombination.However,commonly used HTMs require doping,and the hygroscopicity and corrosiveness of the dopants will destroy the stability of PsCs and hinder their commercialization.Therefore,it is of great significance to develop dopant-free HTMs.展开更多
Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and accept...Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and acceptance of doctors,patients and donors.Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events.This washing preparation makes delivering a precise dose of the enriched microbiota feasible,instead of using the weight of stool.Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration.The toxic reactions that include death,the change in the level of peripheral white blood cells,and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted.The metagenomic next-generation sequencing(NGS)indicated the increasing types and amount of viruses could be washed out during the washing process.Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4,corticosterone,and prostaglandin G2 could be removed by repeated washing.Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess.In conclusion,this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation(WMT)is safer,more precise and more quality-controllable than the crude FMT by manual.展开更多
Scenario forecasting methods have been widely studied in recent years to cope with the wind power uncertainty problem. The main difficulty of this problem is to accurately and comprehensively reflect the time-series c...Scenario forecasting methods have been widely studied in recent years to cope with the wind power uncertainty problem. The main difficulty of this problem is to accurately and comprehensively reflect the time-series characteristics and spatial-temporal correlation of wind power generation. In this paper, the marginal distribution model and the dependence structure are combined to describe these complex characteristics. On this basis, a scenario generation method for multiple wind farms is proposed. For the marginal distribution model, the autoregressive integrated moving average-generalized autoregressive conditional heteroskedasticity-t (ARIMA-GARCH-t) model is proposed to capture the time-series characteristics of wind power generation. For the dependence structure, a time-varying regular vine mixed Copula (TRVMC) model is established to capture the spatial-temporal correlation of multiple wind farms. Based on the data from 8 wind farms in Northwest China, sufficient scenarios are generated. The effectiveness of the scenarios is evaluated in 3 aspects. The results show that the generated scenarios have similar fluctuation characteristics, autocorrelation, and crosscorrelation with the actual wind power sequences.展开更多
基金a grant from the Scientific Research Project of the Bureau of Health of Jiading in Shanghai (No KYXM-2004-11-07)
文摘Objective: To investigate DNA-dependent protein kinase (DNA-PK) expression,and its relationship with lymphat-ic metastasis in colorectal cancer. Methods: Tumor tissues from 60 patients,divided into two groups according to lymphatic metastasis,were immunohistochemically stained to detect the DNA-PK expression including Ku70,Ku80 and PKcs proteins. Results: Positivity of both Ku70 and Ku80 in colorectal cancer was negatively correlated with lymphatic metastasis with an r value of -0.57 and -0.38,respectively. Similar correlation was found between Ku expression,especially Ku70,and long-term survival. PKcs,however,displayed no significant correlation. Statistical analysis failed to detect any correlation between DNA-PK expression,and clinical characteristics,such as age,sex,tumor location,tumor thickness and distant metastasis (P>0.05). Conclusion: DNA-PK expression,especially Ku70 expression,is negatively correlated with lymphatic metastasis,and the survival of patients with colorectal cancer. Ku70 expression may be a potential indicator for the preoperative evaluation,and prognosis in colorectal cancer.
基金supported by the National Natural Science Foundation of China(Nos.51763013 and U20A20128)Jiangxi Provincial Natural Science Foundation(No.20224ACB213002)+1 种基金the Foundation of Jiangxi Educational Committee(No.GJJ200301)Jiangxi Provincial High-level and High-skilled Leading Talents Project.
文摘As the third generation new battery,the power conversion efficiency(PCE)of metal halide perovskite solar cells(PsCs)has increased from 3.8%in 2009 to 25.8%currently certified,which fully shows that they have great research value and development prospect.As one of the main components of high-efficiency PSCs,hole transport materials(HTMs)play an important role in extracting and transporting holes and inhibiting charge recombination.However,commonly used HTMs require doping,and the hygroscopicity and corrosiveness of the dopants will destroy the stability of PsCs and hinder their commercialization.Therefore,it is of great significance to develop dopant-free HTMs.
基金This work was supported by publicly donated Intestine Initiative FoundationPrimary Research&Development Plan of Jiangsu Province(BE2018751)+1 种基金Jiangsu Provincial Medical Innovation Team(Zhang F),National Natural Science Foundation of China(81600417,81670495 and 81873548)China National Center for Clinical Research of Digestive Diseases(201502026).
文摘Fecal microbiota transplantation(FMT)by manual preparation has been applied to treat diseases for thousands of years.However,this method still endures safety risks and challenges the psychological endurance and acceptance of doctors,patients and donors.Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events.This washing preparation makes delivering a precise dose of the enriched microbiota feasible,instead of using the weight of stool.Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration.The toxic reactions that include death,the change in the level of peripheral white blood cells,and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted.The metagenomic next-generation sequencing(NGS)indicated the increasing types and amount of viruses could be washed out during the washing process.Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4,corticosterone,and prostaglandin G2 could be removed by repeated washing.Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess.In conclusion,this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation(WMT)is safer,more precise and more quality-controllable than the crude FMT by manual.
基金This work was supported by the National Key Research and Development Program of China(No.2017YFB0902600).
文摘Scenario forecasting methods have been widely studied in recent years to cope with the wind power uncertainty problem. The main difficulty of this problem is to accurately and comprehensively reflect the time-series characteristics and spatial-temporal correlation of wind power generation. In this paper, the marginal distribution model and the dependence structure are combined to describe these complex characteristics. On this basis, a scenario generation method for multiple wind farms is proposed. For the marginal distribution model, the autoregressive integrated moving average-generalized autoregressive conditional heteroskedasticity-t (ARIMA-GARCH-t) model is proposed to capture the time-series characteristics of wind power generation. For the dependence structure, a time-varying regular vine mixed Copula (TRVMC) model is established to capture the spatial-temporal correlation of multiple wind farms. Based on the data from 8 wind farms in Northwest China, sufficient scenarios are generated. The effectiveness of the scenarios is evaluated in 3 aspects. The results show that the generated scenarios have similar fluctuation characteristics, autocorrelation, and crosscorrelation with the actual wind power sequences.
