为了探索馨香玉兰(Magnolia odoratissima Law et R.Z.Zhou)种子休眠与萌发特性,进行了野外踏察,首次采集到馨香玉兰种子,利用电镜观察了馨香玉兰种子的形态特征,分析了馨香玉兰种子浸提液对白菜种子发芽的影响,测定馨香玉兰种子是否含...为了探索馨香玉兰(Magnolia odoratissima Law et R.Z.Zhou)种子休眠与萌发特性,进行了野外踏察,首次采集到馨香玉兰种子,利用电镜观察了馨香玉兰种子的形态特征,分析了馨香玉兰种子浸提液对白菜种子发芽的影响,测定馨香玉兰种子是否含有萌发抑制物质;利用吸水、沙藏以及GA 3对种子进行处理,研究馨香玉兰种子的休眠特征。结果表明:馨香玉兰种子从母株脱离后具有已发育完整的胚;种子透水性良好,种子休眠不是种皮的不透水性造成的;低温层积后的种子发芽率高,说明馨香玉兰种子具备生理休眠;分别用清水、胚乳滤液、胚乳滤液+GA 3浸泡白菜种子,结果显示,对照组发芽率为100.00%,胚乳滤液组发芽率降低,但胚乳滤液+GA 3组浸泡的白菜种子发芽率比胚乳滤液组有所提升,说明馨香玉兰种子中存在抑制发芽的物质,且抑制效果显著,但外源GA 3能在一定程度上打破这种抑制作用。展开更多
To discuss the inhibitory effect of supramolecular azelaic acid(SAZA)and azelaic acid(AZA)on melanin synthesis.The inhibition rate of tyrosinase activity was tested in vitro.Zebrafish embryos were exposed to SAZA and ...To discuss the inhibitory effect of supramolecular azelaic acid(SAZA)and azelaic acid(AZA)on melanin synthesis.The inhibition rate of tyrosinase activity was tested in vitro.Zebrafish embryos were exposed to SAZA and AZA in different proportions.The development of embryo melanin was observed and quantitatively analyzed.The experimental result showed that both AZA and SAZA had inhibitory effect on tyrosinase,with IC_(50) of 8.34 mmol/L for AZA and 6.56 mmol/L for SAZA.Zebrafish embryo is basically normally developed,with no obvious deformity.Both AZA and SAZA can inhibit the production of melanin,and the SAZA has a better effect in the same amount of experiment.In addition,AZA is able to inhibit the tyrosinase activity and melanin production in zebrafish embryos.The SAZA prepared by cyclodextrin inclusion has shown better water solubility and significantly improved compatibility.展开更多
文摘为了探索馨香玉兰(Magnolia odoratissima Law et R.Z.Zhou)种子休眠与萌发特性,进行了野外踏察,首次采集到馨香玉兰种子,利用电镜观察了馨香玉兰种子的形态特征,分析了馨香玉兰种子浸提液对白菜种子发芽的影响,测定馨香玉兰种子是否含有萌发抑制物质;利用吸水、沙藏以及GA 3对种子进行处理,研究馨香玉兰种子的休眠特征。结果表明:馨香玉兰种子从母株脱离后具有已发育完整的胚;种子透水性良好,种子休眠不是种皮的不透水性造成的;低温层积后的种子发芽率高,说明馨香玉兰种子具备生理休眠;分别用清水、胚乳滤液、胚乳滤液+GA 3浸泡白菜种子,结果显示,对照组发芽率为100.00%,胚乳滤液组发芽率降低,但胚乳滤液+GA 3组浸泡的白菜种子发芽率比胚乳滤液组有所提升,说明馨香玉兰种子中存在抑制发芽的物质,且抑制效果显著,但外源GA 3能在一定程度上打破这种抑制作用。
文摘To discuss the inhibitory effect of supramolecular azelaic acid(SAZA)and azelaic acid(AZA)on melanin synthesis.The inhibition rate of tyrosinase activity was tested in vitro.Zebrafish embryos were exposed to SAZA and AZA in different proportions.The development of embryo melanin was observed and quantitatively analyzed.The experimental result showed that both AZA and SAZA had inhibitory effect on tyrosinase,with IC_(50) of 8.34 mmol/L for AZA and 6.56 mmol/L for SAZA.Zebrafish embryo is basically normally developed,with no obvious deformity.Both AZA and SAZA can inhibit the production of melanin,and the SAZA has a better effect in the same amount of experiment.In addition,AZA is able to inhibit the tyrosinase activity and melanin production in zebrafish embryos.The SAZA prepared by cyclodextrin inclusion has shown better water solubility and significantly improved compatibility.
