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苹果砧木新品种鲁砧1号离体叶片高效不定植株再生
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作者 王海波 王森 +6 位作者 郑文燕 王平 何晓文 常源升 何平 孙清荣 李林光 《果树学报》 CAS CSCD 北大核心 2024年第9期1781-1788,共8页
【目的】建立苹果半矮化砧木新品种鲁砧1号离体叶片高效不定梢再生技术体系,为该砧木快繁和遗传改良奠定技术基础。【方法】以鲁砧1号无菌苗离体叶片为外植体,研究碳源、细胞分裂素种类和质量浓度对叶片不定梢再生的影响;以不定梢为试材... 【目的】建立苹果半矮化砧木新品种鲁砧1号离体叶片高效不定梢再生技术体系,为该砧木快繁和遗传改良奠定技术基础。【方法】以鲁砧1号无菌苗离体叶片为外植体,研究碳源、细胞分裂素种类和质量浓度对叶片不定梢再生的影响;以不定梢为试材,研究基本培养基、蔗糖质量浓度对不定梢生根的影响。【结果】MS添加较低质量浓度(0.6 mg·L^(-1))细胞分裂素(TDZ)时,碳源物质为D-山梨醇的叶片不定梢再生率显著高于蔗糖;其他激素处理下,D-山梨醇和蔗糖之间叶片不定梢再生率无显著差异。两种碳源上的平均每叶不定芽(梢)数,都表现较高细胞分裂素质量浓度处理高于或显著高于较低细胞分裂素质量浓度处理,以TDZ质量浓度为1 mg·L^(-1)时产生的不定芽数最多,平均每叶不定芽数为3.8~4个。在不定芽诱导培养基上,6-苄基氨基嘌呤(BA)比TDZ更容易诱导产生直接伸长生长的不定梢。不定梢生根率和平均每株生根条数,两种基本培养基及两个蔗糖质量浓度之间都表现差异不显著,但以1/4MS基本培养基和20 g·L^(-1)蔗糖组合的生根培养基上获得的生根率和单株生根条数最高,分别超过93%和5.8。【结论】鲁砧1号离体叶片容易诱导再生不定芽和不定梢生根,诱导叶片不定芽再生最佳培养基为MS添加1 mg·L^(-1)TDZ、0.3 mg·L^(-1)IBA和30 g·L^(-1)蔗糖,最佳生根培养基为1/4MS添加0.3~0.5 mg·L^(-1)IBA和20 g·L^(-1)蔗糖。 展开更多
关键词 苹果 矮化砧木 离体叶片 叶片不定梢再生 不定梢生根
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早发性腹膜透析相关性腹膜炎的危险因素及预后研究 被引量:20
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作者 张宁 张蓓茹 +4 位作者 何平 于锐 白瑜 吴岩 张永哲 《中国全科医学》 CAS 北大核心 2019年第14期1696-1701,共6页
背景近年来,随着慢性肾衰竭患者例数逐年增加,腹膜透析作为一种操作简单、对残肾功能保护性好的替代治疗方式,逐渐成为关注的重点。腹膜透析相关性腹膜炎(PDAP)是腹膜透析最主要的并发症,因此寻找PDAP的危险因素对避免或延缓PDAP的发生... 背景近年来,随着慢性肾衰竭患者例数逐年增加,腹膜透析作为一种操作简单、对残肾功能保护性好的替代治疗方式,逐渐成为关注的重点。腹膜透析相关性腹膜炎(PDAP)是腹膜透析最主要的并发症,因此寻找PDAP的危险因素对避免或延缓PDAP的发生、延长腹膜透析患者生存时间至关重要。目的探讨早发性PDAP的危险因素及预后情况。方法选取2008年1月—2016年6月于中国医科大学附属盛京医院行腹膜透析并发生PDAP的患者116例,根据PDAP首次发生时间将患者分为早发性PDAP组(32例)和晚发性PDAP组(84例)。收集患者的临床资料、实验室检查资料,并培养致病菌。对患者进行随访,记录转归情况及生存时间,随访终点为死亡、长久转为血液透析或随访至2017年3月。结果早发性PDAP组违反无菌操作、居住环境为平房所占比例均高于晚发性PDAP组(P<0.05);两组患者操作者情况比较,差异有统计学意义(P<0.05)。早发性PDAP组血红蛋白、清蛋白水平均低于晚发性PDAP组(P<0.05)。116例PDAP患者中,82例进行了腹膜透析液致病菌培养,59例(72.0%)培养结果为阳性,其中,早发性PDAP组14例,晚发性PDAP组45例。两组致病菌分布情况比较,差异无统计学意义(χ~2=0.982,P=0.612)。二元Logistic回归分析结果显示,违反无菌操作〔OR=4.127,95%CI(1.353,12.589)〕、居住环境为平房〔OR=4.785,95%CI(1.455,15.846)〕、低血红蛋白〔OR=0.947,95%CI(0.917,0.978)〕和低清蛋白水平〔OR=0.890,95%CI(0.799,0.978)〕是早发性PDAP的危险因素(P<0.05)。两组患者治疗转归情况比较,差异无统计学意义(χ~2=1.488,P=0.475)。截至随访终点,早发性PDAP组中位生存时间为24.0(11.2,36.8)个月,晚发性PDAP组为40.0(34.4,45.6)个月。Kaplan-Meier曲线分析显示,早发性PDAP组中位生存时间短于晚发性PDAP组(χ~2=7.221,P=0.007)。结论早发性PDAP患者生存时间短,违反无菌操作、居住环境差、低血红蛋白、低清蛋白是早发性PDAP的危险因素,需采取有效措施给予干预,减少早发性PDAP,延长患者生存时间。 展开更多
关键词 腹膜炎 腹膜透析 危险因素 预后
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THE PRELIMINARY STUDY ON LANDSCAPE CULTURE ORIENTATION AND EXPLOITATION OF THE SOUTH DONGTING LAKE WETLAND 被引量:4
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作者 heping WANGBao-zhong 《Chinese Geographical Science》 SCIE CSCD 2002年第3期254-261,共8页
Dongting Lake is internationally a n important wetland.We studied and s ummarized the conception,func-tion,classification and current situation of the wetland-landscape cu lture in this region.The results showed that ... Dongting Lake is internationally a n important wetland.We studied and s ummarized the conception,func-tion,classification and current situation of the wetland-landscape cu lture in this region.The results showed that the cul-ture of Dongting Lake wetland was ric h in diversity,which are the Rice Cul tivation Culture,high-balustrade dwelling,Nuo Culture,Ship Culture,Dragon Bo at Culture,Chu Culture,Ancient Arc hitecture Landscape,Wetland Foodstuff and Cuisine Culture,Civil Art,Historic Heritage and Cultural Relics,Revolutionary Sites and Ruins,and Production and Living Culture,etc.We also evaluated the eco-tourism value of wetland l andscape culture,and analyzed its f eatures and orientation.The results revealed t hat the south Dongting Lake wetland p lays a key role on the Changjiang(Yangtze)River reaches civilization and Chinese civilization,even has great influence on the global civilization.We summ arized that the soul of the south Dongting Lake Cultu re was Wetland Culture,Water Cultur e,Rice Cultivation and Chu Culture.The thoughts,principles and approaches of sustainable exploitation and ut ilization of the wetland landscape c ulture were formulat-ed and suggested. 展开更多
关键词 south dongting lake wetland landscape landscape culture ECO-TOURISM wetland culture
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浅析跨境电商平台即时通讯工具在线翻译特点 被引量:1
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作者 董姣姣 贺萍 《科教文汇》 2018年第30期186-188,共3页
以"一带一路"战略为代表的贸易全球化的进程对跨境电商的时效性提出了挑战,同时对跨境电商平台即时通讯工具在线翻译功能也提高了要求。本文以在C2C跨境电商平台即时通讯工具中,中国商户与拥有不同母语的外国买家交流的话语... 以"一带一路"战略为代表的贸易全球化的进程对跨境电商的时效性提出了挑战,同时对跨境电商平台即时通讯工具在线翻译功能也提高了要求。本文以在C2C跨境电商平台即时通讯工具中,中国商户与拥有不同母语的外国买家交流的话语及其翻译为语料为例,在分析跨境电商在线交流话语特征的基础上,旨在研究跨境电商平台即时通讯工具英汉互译的特点。 展开更多
关键词 跨境电商 即时通讯工具 在线翻译
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Comparison of Phosphorous Absorption, Quality and Yield Between High Oil Corn and Common Corn as Influenced by Phosphorous Application 被引量:1
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作者 heping JINJi-yun +5 位作者 LIWen-juan LIUHai-long HUANGShao-wen WANGXiu-fang WANGLi-chun XIEJia-gui 《Agricultural Sciences in China》 CAS CSCD 2005年第5期376-381,共6页
A field trial was carried out to investigate phosphorous (P) absorption, grain quality and yield between high oil corn and common corn. The results indicated that high oil corn var.Tongyou 1 obtained lower highest P a... A field trial was carried out to investigate phosphorous (P) absorption, grain quality and yield between high oil corn and common corn. The results indicated that high oil corn var.Tongyou 1 obtained lower highest P absorption rate (HAR) and later occurring date of HAR, in comparison with common corn var. Simi 25. The highest HAR and the earliest occurring date of HAR was obtained by the treatments of P45 and P75 in Tongyou 1 and Simi 25 separately; while the total amount of P accumulated by maize plant was achieved by P105 treatment in both varieties. P in grain relied mainly on root uptake at maturation that accounted for 85.7-96.8% and 79.3-84.3% for Tongyou 1 and Simi 25, respectively. Tongyou 1 contained more oil and protein contents, but less starch content with lower grain yield. P application at appropriate rate enhanced contents of protein and fatty acid, but the increment of starch content was neglectable. Acknowledgements This study was financed by the National Key Tech- nologies R & D Program (2004BA520A13), P.R.China. 展开更多
关键词 High oil corn Phosphorous application Phosphorous absorption QUALITY YIELD
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Theories and Methods of Non-optimum Analysis on Systems 被引量:2
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作者 heping 《工程科学(英文版)》 2004年第1期73-80,共8页
The theory of non optimum analysis on systems were discussed in detail. It was pointed out that the main problem of exploring an indefinite systems’ optimum lies in the lack of non optimum analysis on the system. The... The theory of non optimum analysis on systems were discussed in detail. It was pointed out that the main problem of exploring an indefinite systems’ optimum lies in the lack of non optimum analysis on the system. The syndrome and empirical analysis based on the non optimum category of the system were established. At the same time, the non optimum measurement of the system along with non optimum tracing and self organization of the systems was also put forward. 展开更多
关键词 非最优化分析 系统自组织结构 非最优化测量 非最优化综合症 最优化系统 系统控制
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全面贯彻落实“十六大”精神 奋力推进五通桥区发展新跨越
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作者 王和平 Wang heping 《中共乐山市委党校学报》 2002年第6期32-33,共2页
党的十六大深刻阐明了我们党在新世纪举什么旗、走什么路、实现什么目标等重大问题,为我们在全面建设小康社会的新阶段,如何开拓建设有中国特色社会主义新局面勾画了宏伟蓝图,提出了战略目标.
关键词 "十六大"精神 五通桥区 区县经济 乐山市 十六大精神 跨越式发展 全面建设小康社会
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QTL analysis of rice(Oryza sativa L.)tiller angle in a double haploid population derived from anther culture of indica 被引量:2
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作者 QIANQian heping +2 位作者 TENGSheng ZENGDali ZHULihuang 《Chinese Rice Research Newsletter》 1999年第4期1-2,共2页
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The QTL analysis of seedling cold tolerance in a double haploid population derived from anther culture of indica/japonica
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作者 QIANQian ZHENDali +4 位作者 HUANGFang heping ZHENGXiamvu CHENYing ZHULihuang 《Chinese Rice Research Newsletter》 1999年第2期1-2,共2页
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Studies on the Pharmacoknetics of Acetaminophen in Patients with Liver Cirrhosis
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作者 LiuHai-lin HuangXin +1 位作者 HuoLi heping 《胃肠病学》 2000年第B08期117-117,共1页
关键词 药代动力学 对乙酰氨基酚 肝硬化 肝脏疾病
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