In this study, we investigated the possible involvement of nitric oxide pathways in the presynaptic inhibition of acetylcholine release induced by ATP analogs in dogs. We performed the study using HPLC with electroche...In this study, we investigated the possible involvement of nitric oxide pathways in the presynaptic inhibition of acetylcholine release induced by ATP analogs in dogs. We performed the study using HPLC with electrochemical detection and the nitric oxide detection-HPLC system. The amount of acetylcholine released in response to preganglionic stimulation at 5 Hz for 10 min was reduced in a concentration-dependent manner after exposure to 10-7 - 10-4 M α,β-methylene-ATP (α,β-meATP), but not by the P2Y receptor agonist, 2-methyl-thio-ATP (2MeSATP) or the P2X1 receptor agonist, β,γ-methylene-ATP (β,γ-meATP), at the same concentrations. The inhibition of acetylcholine release induced by α,β-meATP was antagonized by: the nonselective P2 receptor antagonist, pyridoxalphosphate-6-azophenyl-2’,4’-disulphonic acid (PPADS);the P2X1, P2X3 and P2X2/3 receptors antagonist, 2’-(or-3’)-O-trinitrophenyl-ATP (TNP-ATP);the neuronal nitric oxide synthase (nNOS) inhibitor, 3-bromo-7-nitroindazole;the soluble guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ);the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethy-limidazoline-1-oxyl-3-oxide (carboxy-PTIO). Exposure to 10-5 M, but not 10-4 M, α,β-meATP, for 30 min increased the levels of , and this increase was antagonized by TNP-ATP and 3-bromo-7-nitroindazole. These results show that P2X receptor activation inhibits stellate ganglionic transmission by reducing acetylcholine release from presynaptic nerve terminals and that this inhibition seems to involve, at least in part, the activation of endogenous NO production and cGMP pathways.展开更多
The indoor mould concentration depends not only on the outdoor mould source but also on the indoor mould source. The mould source is not only on the interior surface but also in the concealed spaces: a crawl space, a ...The indoor mould concentration depends not only on the outdoor mould source but also on the indoor mould source. The mould source is not only on the interior surface but also in the concealed spaces: a crawl space, a beam space, a wall cavity etc. Most detached houses in Japan have crawl spaces under the first floor. The humidity is high from spring to autumn in crawl spaces. Therefore the concentration of mould spore is very high in most crawl spaces. Prefabricated bathrooms called “bath-units” are installed in most Japanese houses. The authors showed in the previous studies that there are infiltration routes between bath-units and walls that the routes may lead chemical compounds from crawl space to indoor space. In this study, the influence of mould infiltration from crawl space upon indoor mould concentration is investigated using a test house which consists of a lavatory and a bathroom. The structure of the house is wooden post-and-beam. The house is airtight and insulated according to the latest building code for save energy. The mould concentrations were measured in the crawl space and the indoor spaces continuously using a mould multi-sampling system with a pump, valves and impingers. Before the measurements, the indoor mould concentration was made low using an air cleaner. The air flow rates were measured using tracer gases. After the exhaust fan was switched on, the mould concentration increased. The number of infiltrated mould was calculated using air flow rates and mould concentrations. The results showed that mould infiltration ratio may be one of the important indicators of indoor air quality.展开更多
文摘In this study, we investigated the possible involvement of nitric oxide pathways in the presynaptic inhibition of acetylcholine release induced by ATP analogs in dogs. We performed the study using HPLC with electrochemical detection and the nitric oxide detection-HPLC system. The amount of acetylcholine released in response to preganglionic stimulation at 5 Hz for 10 min was reduced in a concentration-dependent manner after exposure to 10-7 - 10-4 M α,β-methylene-ATP (α,β-meATP), but not by the P2Y receptor agonist, 2-methyl-thio-ATP (2MeSATP) or the P2X1 receptor agonist, β,γ-methylene-ATP (β,γ-meATP), at the same concentrations. The inhibition of acetylcholine release induced by α,β-meATP was antagonized by: the nonselective P2 receptor antagonist, pyridoxalphosphate-6-azophenyl-2’,4’-disulphonic acid (PPADS);the P2X1, P2X3 and P2X2/3 receptors antagonist, 2’-(or-3’)-O-trinitrophenyl-ATP (TNP-ATP);the neuronal nitric oxide synthase (nNOS) inhibitor, 3-bromo-7-nitroindazole;the soluble guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ);the NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethy-limidazoline-1-oxyl-3-oxide (carboxy-PTIO). Exposure to 10-5 M, but not 10-4 M, α,β-meATP, for 30 min increased the levels of , and this increase was antagonized by TNP-ATP and 3-bromo-7-nitroindazole. These results show that P2X receptor activation inhibits stellate ganglionic transmission by reducing acetylcholine release from presynaptic nerve terminals and that this inhibition seems to involve, at least in part, the activation of endogenous NO production and cGMP pathways.
文摘The indoor mould concentration depends not only on the outdoor mould source but also on the indoor mould source. The mould source is not only on the interior surface but also in the concealed spaces: a crawl space, a beam space, a wall cavity etc. Most detached houses in Japan have crawl spaces under the first floor. The humidity is high from spring to autumn in crawl spaces. Therefore the concentration of mould spore is very high in most crawl spaces. Prefabricated bathrooms called “bath-units” are installed in most Japanese houses. The authors showed in the previous studies that there are infiltration routes between bath-units and walls that the routes may lead chemical compounds from crawl space to indoor space. In this study, the influence of mould infiltration from crawl space upon indoor mould concentration is investigated using a test house which consists of a lavatory and a bathroom. The structure of the house is wooden post-and-beam. The house is airtight and insulated according to the latest building code for save energy. The mould concentrations were measured in the crawl space and the indoor spaces continuously using a mould multi-sampling system with a pump, valves and impingers. Before the measurements, the indoor mould concentration was made low using an air cleaner. The air flow rates were measured using tracer gases. After the exhaust fan was switched on, the mould concentration increased. The number of infiltrated mould was calculated using air flow rates and mould concentrations. The results showed that mould infiltration ratio may be one of the important indicators of indoor air quality.
