BACKGROUND Intraductal papillary neoplasm of the bile duct(IPNB)is a premalignant biliarytype epithelial neoplasm with intraductal papillary or villous growth.Currently reported local palliative therapeutic modalities...BACKGROUND Intraductal papillary neoplasm of the bile duct(IPNB)is a premalignant biliarytype epithelial neoplasm with intraductal papillary or villous growth.Currently reported local palliative therapeutic modalities,including endoscopic nasobiliary drainage,stenting and biliary curettage,endoscopic biliary polypectomy,percutaneous biliary drainage,laser ablation,argon plasma coagulation,photodynamic therapy,and radiofrequency ablation to relieve mechanical obstruction are limited with weaknesses and disadvantages.We have applied percutaneous transhepatic cholangioscopy(PTCS)-assisted biliary polypectomy(PTCS-BP)technique for the management of IPNB including mucin-hypersecreting cast-like and polypoid type tumors since 2010.AIM To assess the technical feasibility,efficacy,and safety of PTCS-BP for local palliative treatment of IPNB.METHODS Patients with mucin-hypersecreting cast-like or polypoid type IPNB and receiving PTCS-BP between September 2010 and December 2019 were included.PTCS-BP was performed by using a half-moon type snare with a soft stainless-steel wire,and the tumor was snared and resected with electrocautery.The primary outcome was its feasibility,indicated by technical success.The secondary outcomes were efficacy,including therapeutic success,curative resection,and clinical success,and safety.RESULTS Five patients(four with mucin-hypersecreting cast-like type and one with polypoid type IPNB)were included.Low-and high-grade intraepithelial neoplasia(HGIN)and recurrent IPNB with invasive carcinoma were observed in one,two,and two patients,respectively.Repeated cholangitis and/or obstructive jaundice were presented in all four patients with mucin-hypersecreting cast-like type IPNB.All five patients achieved technical success of PTCS-BP.Four patients(three with mucin-hypersecreting cast-like type and one with polypoid type IPNB)obtained therapeutic success;one with mucin-hypersecreting cast-like type tumors in the intrahepatic small bile duct and HGIN had residual tumors.All four patients with mucin-hypersecreting IPNB achieved clinical success.The patient with polypoid type IPNB achieved curative resection.There were no PTCS-BP-related serious adverse events.CONCLUSION PTCS-BP appears to be feasible,efficacious,and safe for local palliative treatment of both mucin-hypersecreting cast-like and polypoid type IPNB.展开更多
The selective hydrogenation of highly toxic phenolic compounds to generate alcohols with thermal stability,environmental friendliness,and non-toxicity is of great importance.Herein,a series of Co-based catalysts,named...The selective hydrogenation of highly toxic phenolic compounds to generate alcohols with thermal stability,environmental friendliness,and non-toxicity is of great importance.Herein,a series of Co-based catalysts,named Co@NCNTs,were designed and constructed by direct pyrolysis of hollow ZIF-67(HZIF-67)under H_(2)/Ar atmosphere.The evolution of the catalyst surface from the shell layer assembled by ZIF-67-derived particles to the in situ-grown hollow nitrogen-doped carbon nanotubes(NCNTs)with certain length and density is achieved by adjusting the pyrolysis atmosphere and temperature.Due to the synergistic effects of in situ-formed hollow NCNTs,well-dispersed Co nanoparticles,and intact carbon matrix,the as-prepared Co@NCNTs-0.10-450 catalyst exhibits superior catalytic performance in the hydrogenation of phenolic compounds to alcohols.The turnover frequency value of Co@NCNTs-0.10-450is 3.52 h^(-1),5.9 times higher than that of Co@NCNTs-0.40-450 and 4.5 times higher than that of Co@NCNTs-0.10-550,exceeding most previously reported non-noble metal catalysts.Our findings provide new insights into the development of non-precious metal,efficient,and cost-effective metal-organic framework-derived catalysts for the hydrogenation of phenolic compounds to alcohols.展开更多
BACKGROUND Dysphoria and despondency are prevalent psychological issues in patients undergoing Maintenance Hemodialysis(MHD)that significantly affect their quality of life(QOL).High levels of social support can signif...BACKGROUND Dysphoria and despondency are prevalent psychological issues in patients undergoing Maintenance Hemodialysis(MHD)that significantly affect their quality of life(QOL).High levels of social support can significantly improve the physical and mental well-being of patients undergoing MHD.Currently,there is limited research on how social support mediates the relationship between dysphoria,despondency,and overall QOL in patients undergoing MHD.It is imperative to investigate this mediating effect to mitigate dysphoria and despondency in patients undergoing MHD,ultimately enhancing their overall QOL.AIM To investigate the mediating role of social support in relationships between dysphoria,despondency,and QOL among patients undergoing MHD.METHODS Participants comprised 289 patients undergoing MHD,who were selected using a random sampling approach.The Social Support Rating Scale,Self-Rating Anxiety Scale,Self-Rating Depression Scale,and QOL Scale were administered.Correlation analysis was performed to examine the associations between social support,dysphoria,despondency,and QOL in patients undergoing MHD.To assess the mediating impact of social support on dysphoria,despondency,and QOL in patients undergoing MHD,a bootstrap method was applied.RESULTS Significant correlations among social support,dysphoria,despondency,and quality in patients undergoing MHD were observed(all P<0.01).Dysphoria and despondency negatively correlated with social support and QOL(P<0.01).Dysphoria and despondency had negative predictive impacts on the QOL of patients undergoing MHD(P<0.05).The direct effect of dysphoria on QOL was statistically significant(P<0.05).Social support mediated the relationship between dysphoria and QOL,and this mediating effect was significant(P<0.05).Similarly,the direct effect of despondency on QOL was significant(P<0.05).Moreover,social support played a mediating role between despondency and QOL,with a significant mediating effect(P<0.05).CONCLUSION These findings suggest that social support plays a significant mediating role in the relationship between dysphoria,despondency,and QOL in patients undergoing MHD.展开更多
Teenagers are in the special stage of physical and mental development,and their psychological problems show complex and diverse characteristics.Depression is the most common mental disorder among teenagers.Literary re...Teenagers are in the special stage of physical and mental development,and their psychological problems show complex and diverse characteristics.Depression is the most common mental disorder among teenagers.Literary reading has a certain intervention effect on depression and can be used as an important strategy for psychological intervention.Reading literature works can help teenagers understand and discover themselves from different angles,and then realize the self-recognition and intervention of depression.This paper mainly discusses the causes of depression in teenagers and the intervention principles and strategies of literary reading on depression,aiming to alleviate and eliminate the depressive symptoms of teenagers through literary reading and promote their physical and mental health development.展开更多
Birnessite M_(x)MnO_(2)(M=Na^(+),K^(+),etc.)has emerged as a promising alternative to the classical MnO_(2)material owing to its improved pseudocapacitive performance for energy storage.Understanding their structure–...Birnessite M_(x)MnO_(2)(M=Na^(+),K^(+),etc.)has emerged as a promising alternative to the classical MnO_(2)material owing to its improved pseudocapacitive performance for energy storage.Understanding their structure–property correlation is essential for the development and application of advanced supercapacitors.Herein,we adopt the crystal field theory and density functional simulation to reveal the structural dependence of the pseudocapacitive property of M_(x)MnO_(2).Attributing to the Jahn–Teller effect of Mn^(3+),the bandgap of Kx MnO_(2)can be tuned by changing the x value(i.e.,the Mn(III)/Mn(IV)ratio).Then,we design a narrow-bandgap K 0.25 MnO_(2)(0.84 eV),which affords a high capacitance of 415 F g^(-1)at 1 A g^(-1)and a desirable rate capability of 293 F g^(-1)at 20 A g^(-1).Operando Raman spectroscopy confirms that the Jahn–Teller induced structure evolution of[MnO_(6)]octahedron accounts for the superior pseudocapacitive behavior of K_(0.25)MnO_(2).This finding offers theoretical guidance to the design and application of birnessite materials for pseudocapacitors.展开更多
To meet the high-performance requirements of fifth-generation(5G)and sixth-generation(6G)wireless networks,in particular,ultra-reliable and low-latency communication(URLLC)is considered to be one of the most important...To meet the high-performance requirements of fifth-generation(5G)and sixth-generation(6G)wireless networks,in particular,ultra-reliable and low-latency communication(URLLC)is considered to be one of the most important communication scenarios in a wireless network.In this paper,we consider the effects of the Rician fading channel on the performance of cooperative device-to-device(D2D)communication with URLLC.For better performance,we maximize and examine the system’s minimal rate of D2D communication.Due to the interference in D2D communication,the problem of maximizing the minimum rate becomes non-convex and difficult to solve.To solve this problem,a learning-to-optimize-based algorithm is proposed to find the optimal power allocation.The conventional branch and bound(BB)algorithm are used to learn the optimal pruning policy with supervised learning.Ensemble learning is used to train the multiple classifiers.To address the imbalanced problem,we used the supervised undersampling technique.Comparisons are made with the conventional BB algorithm and the heuristic algorithm.The outcome of the simulation demonstrates a notable performance improvement in power consumption.The proposed algorithm has significantly low computational complexity and runs faster as compared to the conventional BB algorithm and a heuristic algorithm.展开更多
Nucleotide binding site,leucine-rich repeat(NBS-LRR)proteins are the main types of disease resistance proteins in plants,which can perceive plant pathogens.Anthracnose,caused by the fungus Colletotrichum camelliae,is ...Nucleotide binding site,leucine-rich repeat(NBS-LRR)proteins are the main types of disease resistance proteins in plants,which can perceive plant pathogens.Anthracnose,caused by the fungus Colletotrichum camelliae,is one of the most severe diseases in tea plant.Here,we identified an NBS-LRR-encoding gene,CsRPM1,probably conferring resistance of tea plant to C.camelliae.Phylogenetic analysis showed that CsRPM1 was clustered with RPM1 in Arabidopsis and grouped into CC-NBS-LRR(CNL).It contained a signal peptide,a NB-ARC domain,and multiple LRR motifs.RNA-seq and qRT-PCR analysis showed that the transcript level of CsRPM1 was significantly up-regulated after inoculation with C.camelliae.Transiently silencing of CsRPM1 in tea leaves comprised the resistance to C.camelliae,indicating that CsRPM1 was required for plant defense against the pathogen.The subcellular localization showed that CsRPM1 protein was localized in the nucleus,cytoplasm,and cell membrane.Furthermore,the promoter region of CsRPM1 gene contained MeJA-responsive elements,and the expression of CsRPM1 was induced by exogenous methyl jasmonate,suggesting that CsRPM1 gene may be closely related to JA signaling pathway.A total of 17 transcription factors might be responsible for the expression of CsRPM1.Our data indicates that CsRPM1 is required for disease resistance to C.camelliae in tea plant.The characterization of this disease resistance gene sheds light on NLR protein function in tea plant and may facilitate breeding to control the severe anthracnose.展开更多
BACKGROUND： Development of new coronary thrombolytic agents is hot in the market. A new drug, mutated recombinant tissue-type plasminogen activator （rtPAm）, is the product of mutation of tPA by changing binding loc...BACKGROUND： Development of new coronary thrombolytic agents is hot in the market. A new drug, mutated recombinant tissue-type plasminogen activator （rtPAm）, is the product of mutation of tPA by changing binding loci with plasminogen activator inhibitor （PAl）-1 to reduce the degradation. In vitro test has demonstrated that the activity of rtPAm is much higher than rtPA in the absence of PAl. The present study is to observe the efficacy of mutated recombinant tissue-type plasminogen activator （rtPAm） in coronary thrombolytic therapy.METHODS： A total of 30 adult beagles were equally divided into 5 groups after thrombi： vehicle group, urokinase group, rtPAm low-dose group, rtPAm medium-dose group, and rtPAm high-dose group. Thrombolytic effect and myocardial infarction were observed after thrombolytic therapy.RESULTS： In the urokinase group, time to reperfusion was （15.8±3.8） minutes. TIMI 2 flow was demonstrated in 4 beagles, TIMI 3 flow in 2, and re-occlusion in 4 after 90 minutes respectively. In the low- dose rtPAm group, time to reperfusion was （15±4.5） minutes; TIMI 2 flow was demonstrated in 2 beagles, TIMI 3 flow in 4, and re-occlusion in 2 after 90 minutes. In the high-dose rtPAm group, time to reperfusion was （7.5±2.6） minutes. None of the beagles showed re-occlusion after 90 minutes. The infarction areas were （2.1±0.9）% in the medium-dose rtPAm group and （0.7±0.4）% in the high-dose rtPAm group, which decreased significantly than those in the low-dose rtPAm group. The aggregation rate in the medium-dose and high-dose rtPAm groups decreased significantly than that in the urokinase group.CONCLUSION： rtPAm may serve as a thrombolytic agent with platelet-targeted fibrinolysis and antiplatelet aggregation activities.展开更多
文摘BACKGROUND Intraductal papillary neoplasm of the bile duct(IPNB)is a premalignant biliarytype epithelial neoplasm with intraductal papillary or villous growth.Currently reported local palliative therapeutic modalities,including endoscopic nasobiliary drainage,stenting and biliary curettage,endoscopic biliary polypectomy,percutaneous biliary drainage,laser ablation,argon plasma coagulation,photodynamic therapy,and radiofrequency ablation to relieve mechanical obstruction are limited with weaknesses and disadvantages.We have applied percutaneous transhepatic cholangioscopy(PTCS)-assisted biliary polypectomy(PTCS-BP)technique for the management of IPNB including mucin-hypersecreting cast-like and polypoid type tumors since 2010.AIM To assess the technical feasibility,efficacy,and safety of PTCS-BP for local palliative treatment of IPNB.METHODS Patients with mucin-hypersecreting cast-like or polypoid type IPNB and receiving PTCS-BP between September 2010 and December 2019 were included.PTCS-BP was performed by using a half-moon type snare with a soft stainless-steel wire,and the tumor was snared and resected with electrocautery.The primary outcome was its feasibility,indicated by technical success.The secondary outcomes were efficacy,including therapeutic success,curative resection,and clinical success,and safety.RESULTS Five patients(four with mucin-hypersecreting cast-like type and one with polypoid type IPNB)were included.Low-and high-grade intraepithelial neoplasia(HGIN)and recurrent IPNB with invasive carcinoma were observed in one,two,and two patients,respectively.Repeated cholangitis and/or obstructive jaundice were presented in all four patients with mucin-hypersecreting cast-like type IPNB.All five patients achieved technical success of PTCS-BP.Four patients(three with mucin-hypersecreting cast-like type and one with polypoid type IPNB)obtained therapeutic success;one with mucin-hypersecreting cast-like type tumors in the intrahepatic small bile duct and HGIN had residual tumors.All four patients with mucin-hypersecreting IPNB achieved clinical success.The patient with polypoid type IPNB achieved curative resection.There were no PTCS-BP-related serious adverse events.CONCLUSION PTCS-BP appears to be feasible,efficacious,and safe for local palliative treatment of both mucin-hypersecreting cast-like and polypoid type IPNB.
基金the National Key Research and Development Program(2021YFC3001103)the National Natural Science Foundation(22278209,22178165,21921006,22208149)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20211262,BK20220354)a project funded by the priority academic program development of Jiangsu higher education institutions(PAPD)of China。
文摘The selective hydrogenation of highly toxic phenolic compounds to generate alcohols with thermal stability,environmental friendliness,and non-toxicity is of great importance.Herein,a series of Co-based catalysts,named Co@NCNTs,were designed and constructed by direct pyrolysis of hollow ZIF-67(HZIF-67)under H_(2)/Ar atmosphere.The evolution of the catalyst surface from the shell layer assembled by ZIF-67-derived particles to the in situ-grown hollow nitrogen-doped carbon nanotubes(NCNTs)with certain length and density is achieved by adjusting the pyrolysis atmosphere and temperature.Due to the synergistic effects of in situ-formed hollow NCNTs,well-dispersed Co nanoparticles,and intact carbon matrix,the as-prepared Co@NCNTs-0.10-450 catalyst exhibits superior catalytic performance in the hydrogenation of phenolic compounds to alcohols.The turnover frequency value of Co@NCNTs-0.10-450is 3.52 h^(-1),5.9 times higher than that of Co@NCNTs-0.40-450 and 4.5 times higher than that of Co@NCNTs-0.10-550,exceeding most previously reported non-noble metal catalysts.Our findings provide new insights into the development of non-precious metal,efficient,and cost-effective metal-organic framework-derived catalysts for the hydrogenation of phenolic compounds to alcohols.
基金Supported by the Natural Science Foundation Project of Xinjiang Uygur Autonomous Region,No.2021D01C143.
文摘BACKGROUND Dysphoria and despondency are prevalent psychological issues in patients undergoing Maintenance Hemodialysis(MHD)that significantly affect their quality of life(QOL).High levels of social support can significantly improve the physical and mental well-being of patients undergoing MHD.Currently,there is limited research on how social support mediates the relationship between dysphoria,despondency,and overall QOL in patients undergoing MHD.It is imperative to investigate this mediating effect to mitigate dysphoria and despondency in patients undergoing MHD,ultimately enhancing their overall QOL.AIM To investigate the mediating role of social support in relationships between dysphoria,despondency,and QOL among patients undergoing MHD.METHODS Participants comprised 289 patients undergoing MHD,who were selected using a random sampling approach.The Social Support Rating Scale,Self-Rating Anxiety Scale,Self-Rating Depression Scale,and QOL Scale were administered.Correlation analysis was performed to examine the associations between social support,dysphoria,despondency,and QOL in patients undergoing MHD.To assess the mediating impact of social support on dysphoria,despondency,and QOL in patients undergoing MHD,a bootstrap method was applied.RESULTS Significant correlations among social support,dysphoria,despondency,and quality in patients undergoing MHD were observed(all P<0.01).Dysphoria and despondency negatively correlated with social support and QOL(P<0.01).Dysphoria and despondency had negative predictive impacts on the QOL of patients undergoing MHD(P<0.05).The direct effect of dysphoria on QOL was statistically significant(P<0.05).Social support mediated the relationship between dysphoria and QOL,and this mediating effect was significant(P<0.05).Similarly,the direct effect of despondency on QOL was significant(P<0.05).Moreover,social support played a mediating role between despondency and QOL,with a significant mediating effect(P<0.05).CONCLUSION These findings suggest that social support plays a significant mediating role in the relationship between dysphoria,despondency,and QOL in patients undergoing MHD.
文摘Teenagers are in the special stage of physical and mental development,and their psychological problems show complex and diverse characteristics.Depression is the most common mental disorder among teenagers.Literary reading has a certain intervention effect on depression and can be used as an important strategy for psychological intervention.Reading literature works can help teenagers understand and discover themselves from different angles,and then realize the self-recognition and intervention of depression.This paper mainly discusses the causes of depression in teenagers and the intervention principles and strategies of literary reading on depression,aiming to alleviate and eliminate the depressive symptoms of teenagers through literary reading and promote their physical and mental health development.
基金supported by Ministry of Science and Technology of China(2016YFA0201904)National Natural Science Foundation of China(21631002,52172219,51872192)+3 种基金Projects of International Cooperation and Exchanges NSFC(22120102004)Beijing National Laboratory for Molecular Sciences(BNLMS-CXTD-202001)the Jiangsu Natural Science Foundation(No.BK20180002)Fundamental Research Program of Shanxi Province(202103021223019).
文摘Birnessite M_(x)MnO_(2)(M=Na^(+),K^(+),etc.)has emerged as a promising alternative to the classical MnO_(2)material owing to its improved pseudocapacitive performance for energy storage.Understanding their structure–property correlation is essential for the development and application of advanced supercapacitors.Herein,we adopt the crystal field theory and density functional simulation to reveal the structural dependence of the pseudocapacitive property of M_(x)MnO_(2).Attributing to the Jahn–Teller effect of Mn^(3+),the bandgap of Kx MnO_(2)can be tuned by changing the x value(i.e.,the Mn(III)/Mn(IV)ratio).Then,we design a narrow-bandgap K 0.25 MnO_(2)(0.84 eV),which affords a high capacitance of 415 F g^(-1)at 1 A g^(-1)and a desirable rate capability of 293 F g^(-1)at 20 A g^(-1).Operando Raman spectroscopy confirms that the Jahn–Teller induced structure evolution of[MnO_(6)]octahedron accounts for the superior pseudocapacitive behavior of K_(0.25)MnO_(2).This finding offers theoretical guidance to the design and application of birnessite materials for pseudocapacitors.
基金supported in part by the National Natural Science Foundation of China under Grant 61771410in part by the Sichuan Science and Technology Program 2023NSFSC1373in part by Postgraduate Innovation Fund Project of SWUST 23zx7101.
文摘To meet the high-performance requirements of fifth-generation(5G)and sixth-generation(6G)wireless networks,in particular,ultra-reliable and low-latency communication(URLLC)is considered to be one of the most important communication scenarios in a wireless network.In this paper,we consider the effects of the Rician fading channel on the performance of cooperative device-to-device(D2D)communication with URLLC.For better performance,we maximize and examine the system’s minimal rate of D2D communication.Due to the interference in D2D communication,the problem of maximizing the minimum rate becomes non-convex and difficult to solve.To solve this problem,a learning-to-optimize-based algorithm is proposed to find the optimal power allocation.The conventional branch and bound(BB)algorithm are used to learn the optimal pruning policy with supervised learning.Ensemble learning is used to train the multiple classifiers.To address the imbalanced problem,we used the supervised undersampling technique.Comparisons are made with the conventional BB algorithm and the heuristic algorithm.The outcome of the simulation demonstrates a notable performance improvement in power consumption.The proposed algorithm has significantly low computational complexity and runs faster as compared to the conventional BB algorithm and a heuristic algorithm.
基金supported by the Fundamental Research Funds for the Provincial Universities of Zhejiang(2020YQ001)the Open Fund of Key Laboratory of Biology,Genetics and Breeding of Special Economic Animals and Plants,Ministry of Agriculture and Rural Affairs,P.R.China(TZDZW202202)+1 种基金the Zhejiang Provincial Natural Science Foundation(LY22C160001)Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding-Tea Plant(2021C02067-7).
文摘Nucleotide binding site,leucine-rich repeat(NBS-LRR)proteins are the main types of disease resistance proteins in plants,which can perceive plant pathogens.Anthracnose,caused by the fungus Colletotrichum camelliae,is one of the most severe diseases in tea plant.Here,we identified an NBS-LRR-encoding gene,CsRPM1,probably conferring resistance of tea plant to C.camelliae.Phylogenetic analysis showed that CsRPM1 was clustered with RPM1 in Arabidopsis and grouped into CC-NBS-LRR(CNL).It contained a signal peptide,a NB-ARC domain,and multiple LRR motifs.RNA-seq and qRT-PCR analysis showed that the transcript level of CsRPM1 was significantly up-regulated after inoculation with C.camelliae.Transiently silencing of CsRPM1 in tea leaves comprised the resistance to C.camelliae,indicating that CsRPM1 was required for plant defense against the pathogen.The subcellular localization showed that CsRPM1 protein was localized in the nucleus,cytoplasm,and cell membrane.Furthermore,the promoter region of CsRPM1 gene contained MeJA-responsive elements,and the expression of CsRPM1 was induced by exogenous methyl jasmonate,suggesting that CsRPM1 gene may be closely related to JA signaling pathway.A total of 17 transcription factors might be responsible for the expression of CsRPM1.Our data indicates that CsRPM1 is required for disease resistance to C.camelliae in tea plant.The characterization of this disease resistance gene sheds light on NLR protein function in tea plant and may facilitate breeding to control the severe anthracnose.
基金This work was supported by a grant from the National Natural Science Foundation of China （30900459）, and it was also supported by the National 863 Hightechnology Program.
文摘BACKGROUND： Development of new coronary thrombolytic agents is hot in the market. A new drug, mutated recombinant tissue-type plasminogen activator （rtPAm）, is the product of mutation of tPA by changing binding loci with plasminogen activator inhibitor （PAl）-1 to reduce the degradation. In vitro test has demonstrated that the activity of rtPAm is much higher than rtPA in the absence of PAl. The present study is to observe the efficacy of mutated recombinant tissue-type plasminogen activator （rtPAm） in coronary thrombolytic therapy.METHODS： A total of 30 adult beagles were equally divided into 5 groups after thrombi： vehicle group, urokinase group, rtPAm low-dose group, rtPAm medium-dose group, and rtPAm high-dose group. Thrombolytic effect and myocardial infarction were observed after thrombolytic therapy.RESULTS： In the urokinase group, time to reperfusion was （15.8±3.8） minutes. TIMI 2 flow was demonstrated in 4 beagles, TIMI 3 flow in 2, and re-occlusion in 4 after 90 minutes respectively. In the low- dose rtPAm group, time to reperfusion was （15±4.5） minutes; TIMI 2 flow was demonstrated in 2 beagles, TIMI 3 flow in 4, and re-occlusion in 2 after 90 minutes. In the high-dose rtPAm group, time to reperfusion was （7.5±2.6） minutes. None of the beagles showed re-occlusion after 90 minutes. The infarction areas were （2.1±0.9）% in the medium-dose rtPAm group and （0.7±0.4）% in the high-dose rtPAm group, which decreased significantly than those in the low-dose rtPAm group. The aggregation rate in the medium-dose and high-dose rtPAm groups decreased significantly than that in the urokinase group.CONCLUSION： rtPAm may serve as a thrombolytic agent with platelet-targeted fibrinolysis and antiplatelet aggregation activities.
