Objective To preliminarily evaluate the efficacy of the Fu Zheng method(supporting resistance against pathogenic factors)applied by Professor Hong-Feng CHEN in improving the quality of life in patients with triple-neg...Objective To preliminarily evaluate the efficacy of the Fu Zheng method(supporting resistance against pathogenic factors)applied by Professor Hong-Feng CHEN in improving the quality of life in patients with triple-negative breast cancer(TNBC)to collect and organize the traditional Chinese medicine(TCM)formulas applied by Professor Hong-Feng CHEN in the treatment of TNBC in order to explore the patterns in prescription.Methods The Functional Assessment of Cancer Therapy-Breast scale(FACT-B V4.0)were collected before and after treatment;the database and data mining platform for prescribed TCM formulas were constructed using Microsoft SQL Server 2005,and the patterns in drug pairing and combination were summarized by correlation analysis of data mining.Results The formulas improved the mean scores of the patients’physical well-being,social/family well-being,emotional well-being,physical function well-being and additional concerns(P<0.01);the drug combinations and pairs frequently used by Professor Hong-Feng CHEN were summarized.Conclusion The TCM formulas applied by Professor Hong-Feng CHEN can alleviate adverse physiological reactions,improve psychological conditions and improve function in patients.The formulas take spleen invigoration and stomach nourishment as well as blood circulation promotion and stagnation dissipation as the therapeutic principles,with simplicity in prescription and focus on care and protection the foundation of acquired constitution.展开更多
BACKGROUND Granulomatous lobular mastitis(GLM)is a rare benign inflammatory disease of the breast and is classified under comedo mastitis in traditional Chinese medicine(TCM).The etiology of this disease is unknown,an...BACKGROUND Granulomatous lobular mastitis(GLM)is a rare benign inflammatory disease of the breast and is classified under comedo mastitis in traditional Chinese medicine(TCM).The etiology of this disease is unknown,and it mainly occurs in women of childbearing age.The diagnosis depends on histopathological biopsy.At present,there is no systematic and standardized treatment plan for GLM.In the absence of evidence supporting an infectious etiology,affected patients might continue to receive multiple courses of antibiotics and unnecessary surgery.CASE SUMMARY A 37-year-old Chinese woman with a history of coronavirus disease 2019 infection presented with swelling and pain in the left breast.She also had erythema,nodules in the lower extremities,arthritis in both knees,cough,and headache.In the early stage of GLM,the mass was not significantly reduced by conservative treatment with internal application of TCM;hence,surgical treatment was carried out.The aim of postoperative treatment was to drain the pus,eliminate the necrosed tissue,and expand the muscles;fumigation and washing using TCM was applied.CONCLUSION Combined internal and external treatment with TCM,following the principle of“Prioritize internal treatment before ulceration and emphasize external treatment after ulceration”was effective in our patient with GLM.The prognosis was good.We believe that TCM offered valuable therapeutic benefits in this disease.展开更多
Objectives In order to provide theoretical support for clinical standardization and promotion of the use of Hongfen(hydrargyrum oxydatum crudum),a mercury-containing Chinese medicine,as well as to make it easier to ap...Objectives In order to provide theoretical support for clinical standardization and promotion of the use of Hongfen(hydrargyrum oxydatum crudum),a mercury-containing Chinese medicine,as well as to make it easier to apply traditional Chinese topical preparations in a more contemporary manner.Methods We screened the topical research literature on Hongfen.Firstly,we analyzed the current research status of Hongfen statistically in terms of disciplinary distribution,research institutions,funding,publication volume,research types,and research focus of the literature;secondly,we analyzed the research status of Hongfen by MeSH term analysis,abstract and combined with the herbal literature,the indications,usage,dosage,and adverse effects of Hongfen were statistically analyzed,and keyword co-occurrence analysis and data visualization were performed using python+pyecharts.Results Early studies on Hongfen were mainly on the evaluation of the formulation process,while the current studies are mainly focused on clinical effectiveness and safety,with a considerable part of the studies also focused on drug toxicology,metabolism of mercury components target organ toxicity studies,while the basic studies on its mechanism of action are less.The clinical application is mainly focused on chronic ulcerated sores,refractory sinus fistula,and other surgical sore diseases,the use of which is mostly in the form of compounding and adding excipients,the use of which requires consideration of the accumulation of drug toxicity and attention to drug dosage and duration of use in order to reduce the occurrence of clinical side effects.Conclusion Hongfen is a type of mineral medicine that is extremely toxic but has a known curative effect.Its clinical use is low-risk,and its advantages greatly outweigh its drawbacks.Molecular biology technology should be actively carried out to analyze the pharmacological mechanism of Hongfen,which will play a positive role in the transmission and promotion of traditional Chinese medicine topical preparations.展开更多
Ferns and lycophytes have remarkably large genomes.However,little is known about how their genome size evolved in fern lineages.To explore the origins and evolution of chromosome numbers and genome size in ferns,we us...Ferns and lycophytes have remarkably large genomes.However,little is known about how their genome size evolved in fern lineages.To explore the origins and evolution of chromosome numbers and genome size in ferns,we used flow cytometry to measure the genomes of 240 species(255 samples)of extant ferns and lycophytes comprising 27 families and 72 genera,of which 228 species(242 samples)represent new reports.We analyzed correlations among genome size,spore size,chromosomal features,phylogeny,and habitat type preference within a phylogenetic framework.We also applied ANOVA and multinomial logistic regression analysis to preference of habitat type and genome size.Using the phylogeny,we conducted ancestral character reconstruction for habitat types and tested whether genome size changes simultaneously with shifts in habitat preference.We found that 2 C values had weak phylogenetic signal,whereas the base number of chromosomes(x)had a strong phylogenetic signal.Furthermore,our analyses revealed a positive correlation between genome size and chromosome traits,indicating that the base number of chromosomes(x),chromosome size,and polyploidization may be primary contributors to genome expansion in ferns and lycophytes.Genome sizes in different habitat types varied significantly and were significantly correlated with habitat types;specifically,multinomial logistic regression indicated that species with larger 2 C values were more likely to be epiphytes.Terrestrial habitat is inferred to be ancestral for both extant ferns and lycophytes,whereas transitions to other habitat types occurred as the major clades emerged.Shifts in habitat types appear be followed by periods of genomic stability.Based on these results,we inferred that habitat type changes and multiple whole-genome duplications have contributed to the formation of large genomes of ferns and their allies during their evolutionary history.展开更多
Objective:To observe the effect of Jiuyi powder and its active components on the bacterial culture and macrophage phenotypic factors of the chronic refractory wound rat model,and to explore its mechanism of removing d...Objective:To observe the effect of Jiuyi powder and its active components on the bacterial culture and macrophage phenotypic factors of the chronic refractory wound rat model,and to explore its mechanism of removing decay and promoting muscle growth.Methods:SD rats were divided into control group,Jiuyi powder group,Shengdan group,and calcined gypsum group,with 8 rats in each group.MRSA-infected skin lesions and wounds were used to build a model of chronic and difficult-to-heal wounds in rats.After the model was formed,the control group was treated with daily routine nitrofural disinfection and replaced with sterile gauze.On the basis of the control group,quantitative Jiuyi powder,Shengdan powder,and calcined gypsum powder were used for dressing change,once a day for 7 consecutive days.Before and after the last administration,collect rat wound secretions for bacterial culture,inducible monoxide nitrogen synthase content.At the same time,after the last administration,the rat wound tissue was excised for histopathology and immunofluorescence double staining to label macrophages and their M1 phenotype.Results:After the last dressing change,the wound healing of Jiuyi powder group was better than the other groups,and the wound healing rate of each group had significant difference(P<0.05).The histomology showed that the inflammation of Jiuyi powder group was controlled and had a healing trend.After the last drug change,the contents of TNF-α,IL-6 and iNOS in serum of all groups decreased,and the contents of IL-6,TNF-αand iNOS in serum of Jiuyi powder group decreased significantly before and after medication(P<0.05).There was statistical significance in serum IL-6 content between calcined gypsum group and Shengdan group before and after medication(P<0.05).In addition,the results showed that the contents of IL-6 and iNOS in serum of Jiuyi powder group were statistically different from those of the control group(P<0.05).Tissue immunofluorescence double staining showed that the positive rate of M1 macrophages in Jiuyi powder group and Shengdan group was significantly lower than that in control group(P<0.05).The MRSA negative conversion rate of Jiuyi powder group and Shengdan group was better than that of the control group and calcined gypsum group(P<0.05).Conclusion:Jiuyi powder can improve the inflammation of chronic refractory wounds,and has antibacterial,anti-corrosion and myogenic effects.Its mechanism may be related to the inhibition of macrophage M1 phenotype polarization.展开更多
Macrophages are a group of immune cells with highly heterogeneous phenotypes and functions,which run through the whole process of tissue repair.Macrophages are distributed in various organs and tissues of the body,whi...Macrophages are a group of immune cells with highly heterogeneous phenotypes and functions,which run through the whole process of tissue repair.Macrophages are distributed in various organs and tissues of the body,which are a kind of cell population with plasticity and high heterogeneity in the functional state.Under various conditions,it can be transformed into M1-type(classically activated macrophage)and M2-type macrophages(alternatively activated macrophage).It was also found that the phenotypic polarization of macrophages was regulated by a variety of conditions.This review summarizes the existing studies on the effect of macrophage subtypes on wound repair and the mechanism of regulating macrophage phenotype polarization,so as to provide theoretical basis for further studies.展开更多
The field of evolutionary biology has been revolutionized by phylogenomic approaches in the last decades. However, with the ever-increasing size and scope of molecular data collection, conflicting genealogies may be y...The field of evolutionary biology has been revolutionized by phylogenomic approaches in the last decades. However, with the ever-increasing size and scope of molecular data collection, conflicting genealogies may be yielded based on different datasets and analytical approaches, a phenomenon known as cytonuclear discordance(Figure 1A and B).展开更多
基金support from the Shanghai TCM Gushi Surgical School Heritage Research Base Construction Project of Shanghai Health Bureau(ZYSNXD-CC-APGC-JD-002)
文摘Objective To preliminarily evaluate the efficacy of the Fu Zheng method(supporting resistance against pathogenic factors)applied by Professor Hong-Feng CHEN in improving the quality of life in patients with triple-negative breast cancer(TNBC)to collect and organize the traditional Chinese medicine(TCM)formulas applied by Professor Hong-Feng CHEN in the treatment of TNBC in order to explore the patterns in prescription.Methods The Functional Assessment of Cancer Therapy-Breast scale(FACT-B V4.0)were collected before and after treatment;the database and data mining platform for prescribed TCM formulas were constructed using Microsoft SQL Server 2005,and the patterns in drug pairing and combination were summarized by correlation analysis of data mining.Results The formulas improved the mean scores of the patients’physical well-being,social/family well-being,emotional well-being,physical function well-being and additional concerns(P<0.01);the drug combinations and pairs frequently used by Professor Hong-Feng CHEN were summarized.Conclusion The TCM formulas applied by Professor Hong-Feng CHEN can alleviate adverse physiological reactions,improve psychological conditions and improve function in patients.The formulas take spleen invigoration and stomach nourishment as well as blood circulation promotion and stagnation dissipation as the therapeutic principles,with simplicity in prescription and focus on care and protection the foundation of acquired constitution.
文摘BACKGROUND Granulomatous lobular mastitis(GLM)is a rare benign inflammatory disease of the breast and is classified under comedo mastitis in traditional Chinese medicine(TCM).The etiology of this disease is unknown,and it mainly occurs in women of childbearing age.The diagnosis depends on histopathological biopsy.At present,there is no systematic and standardized treatment plan for GLM.In the absence of evidence supporting an infectious etiology,affected patients might continue to receive multiple courses of antibiotics and unnecessary surgery.CASE SUMMARY A 37-year-old Chinese woman with a history of coronavirus disease 2019 infection presented with swelling and pain in the left breast.She also had erythema,nodules in the lower extremities,arthritis in both knees,cough,and headache.In the early stage of GLM,the mass was not significantly reduced by conservative treatment with internal application of TCM;hence,surgical treatment was carried out.The aim of postoperative treatment was to drain the pus,eliminate the necrosed tissue,and expand the muscles;fumigation and washing using TCM was applied.CONCLUSION Combined internal and external treatment with TCM,following the principle of“Prioritize internal treatment before ulceration and emphasize external treatment after ulceration”was effective in our patient with GLM.The prognosis was good.We believe that TCM offered valuable therapeutic benefits in this disease.
基金sponsored by the National Natural Science Foundation of China(No.82104854)the 2nd major clinical research project of the Three-year Action Plan for Promoting Clinical Skills and Clinical Innovation in Municipal Hospitals(SHDC2020CR2051B)+1 种基金National Training Program for Innovative Key Talents of Traditional Chinese Medicine(19-Z-1-13)Shanghai Sailing Program(20YF1449800).
文摘Objectives In order to provide theoretical support for clinical standardization and promotion of the use of Hongfen(hydrargyrum oxydatum crudum),a mercury-containing Chinese medicine,as well as to make it easier to apply traditional Chinese topical preparations in a more contemporary manner.Methods We screened the topical research literature on Hongfen.Firstly,we analyzed the current research status of Hongfen statistically in terms of disciplinary distribution,research institutions,funding,publication volume,research types,and research focus of the literature;secondly,we analyzed the research status of Hongfen by MeSH term analysis,abstract and combined with the herbal literature,the indications,usage,dosage,and adverse effects of Hongfen were statistically analyzed,and keyword co-occurrence analysis and data visualization were performed using python+pyecharts.Results Early studies on Hongfen were mainly on the evaluation of the formulation process,while the current studies are mainly focused on clinical effectiveness and safety,with a considerable part of the studies also focused on drug toxicology,metabolism of mercury components target organ toxicity studies,while the basic studies on its mechanism of action are less.The clinical application is mainly focused on chronic ulcerated sores,refractory sinus fistula,and other surgical sore diseases,the use of which is mostly in the form of compounding and adding excipients,the use of which requires consideration of the accumulation of drug toxicity and attention to drug dosage and duration of use in order to reduce the occurrence of clinical side effects.Conclusion Hongfen is a type of mineral medicine that is extremely toxic but has a known curative effect.Its clinical use is low-risk,and its advantages greatly outweigh its drawbacks.Molecular biology technology should be actively carried out to analyze the pharmacological mechanism of Hongfen,which will play a positive role in the transmission and promotion of traditional Chinese medicine topical preparations.
基金the National Natural Science Foundation of China(grant number 31870188,31800174,31700172,41571056)to Wang,Shen,Wang and XingShanghai Landscaping and City Appearance Administrative Bureau of China,Scientific Research Grants(G182411)to Yan+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(grant number XDA13020603,XDA13020500)to Chen and JianGuangdong Natural Science Foundation(grant number 2015A030308015)to Wang。
文摘Ferns and lycophytes have remarkably large genomes.However,little is known about how their genome size evolved in fern lineages.To explore the origins and evolution of chromosome numbers and genome size in ferns,we used flow cytometry to measure the genomes of 240 species(255 samples)of extant ferns and lycophytes comprising 27 families and 72 genera,of which 228 species(242 samples)represent new reports.We analyzed correlations among genome size,spore size,chromosomal features,phylogeny,and habitat type preference within a phylogenetic framework.We also applied ANOVA and multinomial logistic regression analysis to preference of habitat type and genome size.Using the phylogeny,we conducted ancestral character reconstruction for habitat types and tested whether genome size changes simultaneously with shifts in habitat preference.We found that 2 C values had weak phylogenetic signal,whereas the base number of chromosomes(x)had a strong phylogenetic signal.Furthermore,our analyses revealed a positive correlation between genome size and chromosome traits,indicating that the base number of chromosomes(x),chromosome size,and polyploidization may be primary contributors to genome expansion in ferns and lycophytes.Genome sizes in different habitat types varied significantly and were significantly correlated with habitat types;specifically,multinomial logistic regression indicated that species with larger 2 C values were more likely to be epiphytes.Terrestrial habitat is inferred to be ancestral for both extant ferns and lycophytes,whereas transitions to other habitat types occurred as the major clades emerged.Shifts in habitat types appear be followed by periods of genomic stability.Based on these results,we inferred that habitat type changes and multiple whole-genome duplications have contributed to the formation of large genomes of ferns and their allies during their evolutionary history.
基金Science and technology innovation plan of Shanghai Municipal Science and Technology Commission(No.20YF1449800)Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Leading Scholar ofMedicine Cultivation Program(No.LYTD-74)。
文摘Objective:To observe the effect of Jiuyi powder and its active components on the bacterial culture and macrophage phenotypic factors of the chronic refractory wound rat model,and to explore its mechanism of removing decay and promoting muscle growth.Methods:SD rats were divided into control group,Jiuyi powder group,Shengdan group,and calcined gypsum group,with 8 rats in each group.MRSA-infected skin lesions and wounds were used to build a model of chronic and difficult-to-heal wounds in rats.After the model was formed,the control group was treated with daily routine nitrofural disinfection and replaced with sterile gauze.On the basis of the control group,quantitative Jiuyi powder,Shengdan powder,and calcined gypsum powder were used for dressing change,once a day for 7 consecutive days.Before and after the last administration,collect rat wound secretions for bacterial culture,inducible monoxide nitrogen synthase content.At the same time,after the last administration,the rat wound tissue was excised for histopathology and immunofluorescence double staining to label macrophages and their M1 phenotype.Results:After the last dressing change,the wound healing of Jiuyi powder group was better than the other groups,and the wound healing rate of each group had significant difference(P<0.05).The histomology showed that the inflammation of Jiuyi powder group was controlled and had a healing trend.After the last drug change,the contents of TNF-α,IL-6 and iNOS in serum of all groups decreased,and the contents of IL-6,TNF-αand iNOS in serum of Jiuyi powder group decreased significantly before and after medication(P<0.05).There was statistical significance in serum IL-6 content between calcined gypsum group and Shengdan group before and after medication(P<0.05).In addition,the results showed that the contents of IL-6 and iNOS in serum of Jiuyi powder group were statistically different from those of the control group(P<0.05).Tissue immunofluorescence double staining showed that the positive rate of M1 macrophages in Jiuyi powder group and Shengdan group was significantly lower than that in control group(P<0.05).The MRSA negative conversion rate of Jiuyi powder group and Shengdan group was better than that of the control group and calcined gypsum group(P<0.05).Conclusion:Jiuyi powder can improve the inflammation of chronic refractory wounds,and has antibacterial,anti-corrosion and myogenic effects.Its mechanism may be related to the inhibition of macrophage M1 phenotype polarization.
基金Project Funded by the National Natural Science Foundation of China.(No.8157150938)Project Funded by Longhua Hospital(No.D-74).
文摘Macrophages are a group of immune cells with highly heterogeneous phenotypes and functions,which run through the whole process of tissue repair.Macrophages are distributed in various organs and tissues of the body,which are a kind of cell population with plasticity and high heterogeneity in the functional state.Under various conditions,it can be transformed into M1-type(classically activated macrophage)and M2-type macrophages(alternatively activated macrophage).It was also found that the phenotypic polarization of macrophages was regulated by a variety of conditions.This review summarizes the existing studies on the effect of macrophage subtypes on wound repair and the mechanism of regulating macrophage phenotype polarization,so as to provide theoretical basis for further studies.
基金supported by South China Botanical Garden, Chinese Academy of Sciences (QNXM-202301)International Partnership Program of the Chinese Academy of Sciences (068GJHZ2022055FN)+1 种基金Department of Science and Technology of Guangzhou (202102080496)Department of Science and Technology of Guangdong Province (2022A0505030007)。
文摘The field of evolutionary biology has been revolutionized by phylogenomic approaches in the last decades. However, with the ever-increasing size and scope of molecular data collection, conflicting genealogies may be yielded based on different datasets and analytical approaches, a phenomenon known as cytonuclear discordance(Figure 1A and B).
