The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. scleroti...The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. sclerotiorum degrades cellulose remain elusive. Here, we unveil a novel secretory cellobiohydrolase, SsdchA, characterized by a signal peptide and a Glyco_hydro_7(GH7) domain. SsdchA exhibits a robust expression of during early infection stages. Interestingly, colony morphology and growth rates remain unaffected across the wild-type, SsdchA deletion strains and SsdchA overexpression strains on potato dextrose agar(PDA) medium. Nevertheless, the pathogenicity and cellobiohydrolase activity decreased in the SsdchA deletion strains, but enhanced in the SsdchA overexpression strains. Moreover,the heterologous expression of SsdchA in Arabidopsis thaliana leads to reduced cellulose content and heightened susceptibility to S. sclerotiorum. Collectively, our data underscore the pivotal role of the novel cellobiohydrolase SsdchA in the pathogenicity of S. sclerotiorum.展开更多
With the increasing demand for environmentally friendly,safe,preservative and intelligent food packaging,there is a growing trend towards using plant-derived natural colorants that posses green,non-toxic,antioxidant,a...With the increasing demand for environmentally friendly,safe,preservative and intelligent food packaging,there is a growing trend towards using plant-derived natural colorants that posses green,non-toxic,antioxidant,antibacterial,and pH-sensitive properties.As a result,the development of active intelligent packaging films containing plant-derived natural colorants has become a research priority in the realm of food packaging.As a novel packaging approach,it can serve as an active and intelligent packaging system to prolong shelf life and monitor food quality.On the basis of introducing several widely used natural colorants derived from plants,this review examines the preparation,structural characterization,physical properties,and functional aspects of these plant-derived pigments.The preparation procedures of various film forming substrates and natural pigment based films are also comprehensively discussed.Furthermore,the utilization of natural pigment-based films as active and intelligent packaging materials in food is discussed in depth,providing valuable insights into the future development of this cuttingedge research area.展开更多
基金financially supported by the National Nature Science Foundation of China (32372077)the Project of Chongqing Science and Technology Commission (CSTB2023NSCQ-MSX0355)the Fundamental Research Funds for the Central Universities (SWU120075)。
文摘The necrotrophic fungus, Sclerotinia sclerotiorum, employs an array of cell wall-degrading enzymes(CWDEs), including cellulase, to dismantle host cell walls. However, the molecular mechanisms through which S. sclerotiorum degrades cellulose remain elusive. Here, we unveil a novel secretory cellobiohydrolase, SsdchA, characterized by a signal peptide and a Glyco_hydro_7(GH7) domain. SsdchA exhibits a robust expression of during early infection stages. Interestingly, colony morphology and growth rates remain unaffected across the wild-type, SsdchA deletion strains and SsdchA overexpression strains on potato dextrose agar(PDA) medium. Nevertheless, the pathogenicity and cellobiohydrolase activity decreased in the SsdchA deletion strains, but enhanced in the SsdchA overexpression strains. Moreover,the heterologous expression of SsdchA in Arabidopsis thaliana leads to reduced cellulose content and heightened susceptibility to S. sclerotiorum. Collectively, our data underscore the pivotal role of the novel cellobiohydrolase SsdchA in the pathogenicity of S. sclerotiorum.
文摘With the increasing demand for environmentally friendly,safe,preservative and intelligent food packaging,there is a growing trend towards using plant-derived natural colorants that posses green,non-toxic,antioxidant,antibacterial,and pH-sensitive properties.As a result,the development of active intelligent packaging films containing plant-derived natural colorants has become a research priority in the realm of food packaging.As a novel packaging approach,it can serve as an active and intelligent packaging system to prolong shelf life and monitor food quality.On the basis of introducing several widely used natural colorants derived from plants,this review examines the preparation,structural characterization,physical properties,and functional aspects of these plant-derived pigments.The preparation procedures of various film forming substrates and natural pigment based films are also comprehensively discussed.Furthermore,the utilization of natural pigment-based films as active and intelligent packaging materials in food is discussed in depth,providing valuable insights into the future development of this cuttingedge research area.
