The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis.The cells were exposed to differen...The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis.The cells were exposed to different concentrations of luteolin(0,40,80,120,160,200,240M)for 24,48,and 72 h respectively.The influence of luteolin on proliferation of Eca109 cells was detected using MTT assay.Eca109 cells were then treated with luteolin(0,40,160,240M)for 24 h.The effect of luteolin on cell cycle progression and apoptosis was assayed by using flow cytometry(FCM).Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively.The results showed that luteolin could inhibit the proliferation of Eca109 cells at all concentrations in a time-dependent manner and the relative inhibition rate showed an inverted U-shaped association with the concentration of luteolin.Further,the cell cycle was arrested in the S phase following treatment with luteolin.Apoptosis analysis indicated that luteolin could induce the apoptosis of Eca109 cells across the three concentration groups,which exhibited a trend of first promotional and then inhibitory with the increases in luteolin concentration.The effect of luteolin on the mRNA and protein expression of caspase 9 and caspase3 first manifested as promotion,then inhibition.Therefore,luteolin may serve a role in promoting cell apoptosis by inducing Eca109 cell apoptosis that involves the expression of caspase3,caspase9 mRNA and protein.This study provides theoretical basis for further study and clinical application of luteolin.The specific mechanism has not yet been clarified and the other activation pathways inducing apoptosis need to be further studied.展开更多
Recent studies have explored the spatial transcriptomics patterns of Alzheimer's disease(AD)brain by spatial sequencing in mouse models,enabling the identification of unique genome-wide transcriptomic features ass...Recent studies have explored the spatial transcriptomics patterns of Alzheimer's disease(AD)brain by spatial sequencing in mouse models,enabling the identification of unique genome-wide transcriptomic features associated with different spatial regions and pathological status.However,the dynamics of gene interactions that occur during amyloid-βaccumulation remain largely unknown.In this study,we performed analyses on ligand-receptor communication,transcription factor regulatory network,and spot-specific network to reveal the dependence and the dynamics of gene associations/interactions on spatial regions and pathological status with mouse and human brains.We first used a spatial transcriptomics dataset of the AppNL-G-F knock-in AD and wild-type mouse model.We revealed 17 ligand-receptor pairs with opposite tendencies throughout the amyloid-βaccumulation process and showed the specific ligand-receptor interactions across the hippocampus layers at different extents of pathological changes.We then identified nerve function related transcription factors in the hippocampus and entorhinal cortex,as well as genes with different transcriptomic association degrees in AD versus wild-type mice.Finally,another independent spatial transcriptomics dataset from different AD mouse models and human single-nuclei RNA-seq data/AlzData database were used for validation.This is the first study to identify various gene associations throughout amyloid-βaccumulation based on spatial transcriptomics,establishing the foundations to reveal advanced and in-depth AD etiology from a novel perspective based on the comprehensive analyses of gene interactions that are spatio-temporal dependent.展开更多
Rubbers have been well accepted for modifying brittle epoxies but rubber modified epoxies usually posses lowered tensile strength though enhanced ductility and fracture resistance. In this work, a polyethylene glycol ...Rubbers have been well accepted for modifying brittle epoxies but rubber modified epoxies usually posses lowered tensile strength though enhanced ductility and fracture resistance. In this work, a polyethylene glycol （PEG-4000） is used to modify diglycidyl ether of bisphenol A/methyltetrahydrophthalic anhydride system for enhancing cryogenic tensile strength, ductility and impact resistance. The results display that the cryogenic tensile strength, ductility （failure strain） and fracture resistance （impact strength） are all enhanced for the modified epoxy system at proper PEG contents. The maximum tensile strength （127.8 MPa） at the cryogenic temperature （77 K） with an improvement of 30.1% is observed for the modified system with the 15 wt% PEG content. The ductility and impact resistance at both room temperature and cryogenic temperature are all improved for the modified epoxy system with proper PEG-4000 contents. These observations are explained by the positron annihilation lifetime spectroscopy results and scanning electron microscopy results. Moreover, the glass transition temperature decreases slightly with increasing PEG content.展开更多
基金Thanks for the support of the National Natural Science Foundation of China(https://isisn.nsfc.gov.cn/egrantindex/funcindex/prjsearch-list,grant nos.:81673147,81372985)he Fundamental Research Funds for the Central Universities(grant no.:2242017K40035)Postgraduate Research&Practice Innovation Program of Jiangsu Province(grant no.:SJCX170070).
文摘The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis.The cells were exposed to different concentrations of luteolin(0,40,80,120,160,200,240M)for 24,48,and 72 h respectively.The influence of luteolin on proliferation of Eca109 cells was detected using MTT assay.Eca109 cells were then treated with luteolin(0,40,160,240M)for 24 h.The effect of luteolin on cell cycle progression and apoptosis was assayed by using flow cytometry(FCM).Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively.The results showed that luteolin could inhibit the proliferation of Eca109 cells at all concentrations in a time-dependent manner and the relative inhibition rate showed an inverted U-shaped association with the concentration of luteolin.Further,the cell cycle was arrested in the S phase following treatment with luteolin.Apoptosis analysis indicated that luteolin could induce the apoptosis of Eca109 cells across the three concentration groups,which exhibited a trend of first promotional and then inhibitory with the increases in luteolin concentration.The effect of luteolin on the mRNA and protein expression of caspase 9 and caspase3 first manifested as promotion,then inhibition.Therefore,luteolin may serve a role in promoting cell apoptosis by inducing Eca109 cell apoptosis that involves the expression of caspase3,caspase9 mRNA and protein.This study provides theoretical basis for further study and clinical application of luteolin.The specific mechanism has not yet been clarified and the other activation pathways inducing apoptosis need to be further studied.
基金supported by grants from the National Natural Science Foundation of China (No.81902277)the National Key R&D Program of China (No.2017YFC1001100).
文摘Recent studies have explored the spatial transcriptomics patterns of Alzheimer's disease(AD)brain by spatial sequencing in mouse models,enabling the identification of unique genome-wide transcriptomic features associated with different spatial regions and pathological status.However,the dynamics of gene interactions that occur during amyloid-βaccumulation remain largely unknown.In this study,we performed analyses on ligand-receptor communication,transcription factor regulatory network,and spot-specific network to reveal the dependence and the dynamics of gene associations/interactions on spatial regions and pathological status with mouse and human brains.We first used a spatial transcriptomics dataset of the AppNL-G-F knock-in AD and wild-type mouse model.We revealed 17 ligand-receptor pairs with opposite tendencies throughout the amyloid-βaccumulation process and showed the specific ligand-receptor interactions across the hippocampus layers at different extents of pathological changes.We then identified nerve function related transcription factors in the hippocampus and entorhinal cortex,as well as genes with different transcriptomic association degrees in AD versus wild-type mice.Finally,another independent spatial transcriptomics dataset from different AD mouse models and human single-nuclei RNA-seq data/AlzData database were used for validation.This is the first study to identify various gene associations throughout amyloid-βaccumulation based on spatial transcriptomics,establishing the foundations to reveal advanced and in-depth AD etiology from a novel perspective based on the comprehensive analyses of gene interactions that are spatio-temporal dependent.
基金support of the National Natural Science Foundation of China(Nos. 51073169,10972216 and 11002141)
文摘Rubbers have been well accepted for modifying brittle epoxies but rubber modified epoxies usually posses lowered tensile strength though enhanced ductility and fracture resistance. In this work, a polyethylene glycol （PEG-4000） is used to modify diglycidyl ether of bisphenol A/methyltetrahydrophthalic anhydride system for enhancing cryogenic tensile strength, ductility and impact resistance. The results display that the cryogenic tensile strength, ductility （failure strain） and fracture resistance （impact strength） are all enhanced for the modified epoxy system at proper PEG contents. The maximum tensile strength （127.8 MPa） at the cryogenic temperature （77 K） with an improvement of 30.1% is observed for the modified system with the 15 wt% PEG content. The ductility and impact resistance at both room temperature and cryogenic temperature are all improved for the modified epoxy system with proper PEG-4000 contents. These observations are explained by the positron annihilation lifetime spectroscopy results and scanning electron microscopy results. Moreover, the glass transition temperature decreases slightly with increasing PEG content.
