In this article, a detection method for organic explosives by capillary electrophoresis (CE) is developed based on previous detection techniques. Firstly, a buffer solution consisting of 50 mmol·L-1 sodium dodecy...In this article, a detection method for organic explosives by capillary electrophoresis (CE) is developed based on previous detection techniques. Firstly, a buffer solution consisting of 50 mmol·L-1 sodium dodecyl sulfate (SDS), 20 mmol·L-1 sodium tetraborate and 5% methanol was prepared and the UV detection in this buffer solution was conducted for three common organic explosives, including TNT, DNT and PETN. Then, the capillary UV detection method was investigated in terms of the transition time repeatability, the linear relationship between mass concentration and peak area and the limit of detection. The results revealed good reliability and stability of this method. In addition, these samples were characterized by photodiode array detector (PDA) to verify the qualitative results of UV detection.展开更多
Powder dusting method is the most practically useful approach for latent fingerprint development in the crime scene. Herein, a general powder dusting method has been explored for latent fingerprint development based o...Powder dusting method is the most practically useful approach for latent fingerprint development in the crime scene. Herein, a general powder dusting method has been explored for latent fingerprint development based on aggregation-induced emission luminogens(AIEgens). A series of tetraphenylethene(TPE) derivatives with multiple diphenylamine(DPA), namely, TPE-DPA,TPE-2 DPA and TPE-4 DPA, were selected as candidates to dope with magnetic powders and applied for latent fingerprint development. After screening, the magnetic powder 3 doped with TPE-4 DPA proves to be the best, in terms of fluorescent intensity, resolution and adhesiveness. Afterwards, the magnetic powder 3 was applied for visualization of latent fingerprint on various smooth and porous substrates, including glass, stainless steel, leaf, ceram, plastic bag, lime wall, wood and paper money.Specific details, such as island, core, termination and bifurcation, can be clearly observed for the fluorescent fingerprint images.展开更多
Recently,toxin and narcotics detection techniques have been developed in China.A number of new technologies have been applied.The pretreatment technologies such as solid‑phase extraction(SPE)techniques,solid‑phase mic...Recently,toxin and narcotics detection techniques have been developed in China.A number of new technologies have been applied.The pretreatment technologies such as solid‑phase extraction(SPE)techniques,solid‑phase microextraction(SPME)techniques,hollow‑fiber liquid‑phase microextraction(HF‑LPME)techniques,etc.,have matured,and thus have greatly improved both the accuracy and efficiency of the analysis of toxins and narcotics as well as the technical system of analysis.Based on analyses conducted using the traditional methods of gas chromatography(GC)as well as other conventional methods,various new methods of analysis have been gradually discovered and developed.For instance,ultra‑performance liquid chromatography(UPLC)‑mass spectrometry(MS)and other combination technologies have enriched the variety of detection methods that are available to researchers.These highly precise methods are developed on the basis of and as extensions of traditional applications and still draw the attention of researchers from a variety of disciplines.展开更多
In accordance with the research specifications and guidelines in China,we developed a novel experimental method to detect new piperazine‑type drugs,such as 1‑(3‑trifluoromethylphenyl)piperazine and 1‑(3‑chlorophenyl)p...In accordance with the research specifications and guidelines in China,we developed a novel experimental method to detect new piperazine‑type drugs,such as 1‑(3‑trifluoromethylphenyl)piperazine and 1‑(3‑chlorophenyl)piperazine.In this study,a new pretreatment method and gas chromatography(GC)/nitrogen–phosphorus detector detection technique were used to characterize these two kinds of drugs in urine and blood samples.For the purpose of isolation of these trace drugs from the samples,liquid‑liquid extraction/solid‑phase extraction was modified and validated for this specific study.The pretreatment method presented in this paper has many advantages,such as high recovery rate,high extraction efficiency,high detection sensitivity,low limit of detection,and simple operation.The GC/NPD instrument is popular in most laboratories because it can meet the routine requirements of forensic science.All these aspects make this combination of sample pretreatment and GC/NPD technique the most suitable choice for drug detection in biological samples.展开更多
2,4,6-trinitrotoluene(TNT)is a commonly used explosive.It is not only a threat to public safety but also causes environmental pollution,affecting human health.However,at this stage of TNT detection,technology cannot m...2,4,6-trinitrotoluene(TNT)is a commonly used explosive.It is not only a threat to public safety but also causes environmental pollution,affecting human health.However,at this stage of TNT detection,technology cannot meet the demands of the current situation.To acquire a new method devoted to the study of fast and quantitative detection of TNT.It combines the molecular imprinting technique(MIT)with surface plasmon resonance(SPR)technology for high sensitivity.In this study,a molecularly imprinted polymer(MIP)film for the detection of TNT was synthesized by heat in acetonitrile at 60°C,using the TNT imprinting molecule and azobisisobutyronitrile(AEBN)as initiators.In the present work,there are many factors that can influence the elution efficiency,such as raw material ratios,fore-reaction time,reaction time,etc.The polymers have the highest elution efficiency when raw material mole ratios[n(TNT):n methacrylic acid(MAA):n ethylene glycol dimethylacrylate(EGDMA)]were 1:4:8;the MIP sensor could detect a TNT concentration as low as 1x10-10 M.Compared to the blank polymer with the same chemical composition,the imprinted polymer had higher binding efficiency and higher selectivity.展开更多
A gold nanoparticle‑enhanced surface plasmon resonance(SPR)aptasensor was developed for high‑specificity and high‑sensitivity detection of 2,4,6‑trinitrotoluene(TNT).Self‑assembly film‑forming technology was used to m...A gold nanoparticle‑enhanced surface plasmon resonance(SPR)aptasensor was developed for high‑specificity and high‑sensitivity detection of 2,4,6‑trinitrotoluene(TNT).Self‑assembly film‑forming technology was used to modify the gold surface of the sensor chip with 2,4,6‑trinitrophenyl glycine,a TNT analogue,using polyethylene glycol to which the thiol group and carboxyl group are attached.Aptamer‑gold nanoparticle complexes were formed through Au‑S bonding.To detect TNT,the samples were incubated with the aptamer‑gold nanoparticle complexes,and the solution competition method was applied through the SPR aptasensor.The results showed that the SPR aptasensor achieved fast,real‑time detection of TNT.This gold nanoparticle‑enhanced SPR aptasensor is suitable for TNT detection in the field of public safety and environmental monitoring.展开更多
文摘In this article, a detection method for organic explosives by capillary electrophoresis (CE) is developed based on previous detection techniques. Firstly, a buffer solution consisting of 50 mmol·L-1 sodium dodecyl sulfate (SDS), 20 mmol·L-1 sodium tetraborate and 5% methanol was prepared and the UV detection in this buffer solution was conducted for three common organic explosives, including TNT, DNT and PETN. Then, the capillary UV detection method was investigated in terms of the transition time repeatability, the linear relationship between mass concentration and peak area and the limit of detection. The results revealed good reliability and stability of this method. In addition, these samples were characterized by photodiode array detector (PDA) to verify the qualitative results of UV detection.
基金supported by the Opening Project Fund of Shanghai Key Laboratory of Criminal Scene Evidence (201123417049)the Academician Foundation of the Ministry of Public Security of China (2011-23214203, 23215243, 23317015)+4 种基金Program for Young Innovative Research Team in China University of Political Science and Law (18CXTD09, 16CXTD05)the National Natural Science Foundation of China (21788102)the Research Grants Council of Hong Kong (16308116, C2014-15G, A-HKUST605/16, C6009-17G)the Nissan Chemical Industries Ltd., the AIEgen Biotech Co. Ltd., the Innovation and Technology Commission (ITC-CNERC14SC01)the Science and Technology Plan of Shenzhen (JCY20160229205601482)
文摘Powder dusting method is the most practically useful approach for latent fingerprint development in the crime scene. Herein, a general powder dusting method has been explored for latent fingerprint development based on aggregation-induced emission luminogens(AIEgens). A series of tetraphenylethene(TPE) derivatives with multiple diphenylamine(DPA), namely, TPE-DPA,TPE-2 DPA and TPE-4 DPA, were selected as candidates to dope with magnetic powders and applied for latent fingerprint development. After screening, the magnetic powder 3 doped with TPE-4 DPA proves to be the best, in terms of fluorescent intensity, resolution and adhesiveness. Afterwards, the magnetic powder 3 was applied for visualization of latent fingerprint on various smooth and porous substrates, including glass, stainless steel, leaf, ceram, plastic bag, lime wall, wood and paper money.Specific details, such as island, core, termination and bifurcation, can be clearly observed for the fluorescent fingerprint images.
基金supported by the Opening Project of Key Laboratory of Evidence Science at China University of Political Science and Law,Ministry of Education(2012KFKT07)the Program for Young Innovative Research Team at China University of Political Science and Law(1000‑10814344)Academician Foundation of the Ministry of Public Security of the People’s Republic of China(no.2011‑23210044,2011‑23211119,23212052).
文摘Recently,toxin and narcotics detection techniques have been developed in China.A number of new technologies have been applied.The pretreatment technologies such as solid‑phase extraction(SPE)techniques,solid‑phase microextraction(SPME)techniques,hollow‑fiber liquid‑phase microextraction(HF‑LPME)techniques,etc.,have matured,and thus have greatly improved both the accuracy and efficiency of the analysis of toxins and narcotics as well as the technical system of analysis.Based on analyses conducted using the traditional methods of gas chromatography(GC)as well as other conventional methods,various new methods of analysis have been gradually discovered and developed.For instance,ultra‑performance liquid chromatography(UPLC)‑mass spectrometry(MS)and other combination technologies have enriched the variety of detection methods that are available to researchers.These highly precise methods are developed on the basis of and as extensions of traditional applications and still draw the attention of researchers from a variety of disciplines.
基金supported by Fundamental research fund of Institute of Forensic Science,Ministry of Public Security(The detection of new drugs analysis in biological samples.No.2012JB005)Academician Foundation of the Ministry of Public Security of the People’s Republic of China(No.2011-23214203,23215243,23317015)+2 种基金Beijing Municipal Education Commission University Science and Technology Park Construction Project(2011-08111603)Program for Young Innovative Research Team in China University of Political Science and Law(2014CXTD04,16CXTD05)the opening project fund of Shanghai Key Laboratory of Criminal Scene Evidence(2011-23417049).
文摘In accordance with the research specifications and guidelines in China,we developed a novel experimental method to detect new piperazine‑type drugs,such as 1‑(3‑trifluoromethylphenyl)piperazine and 1‑(3‑chlorophenyl)piperazine.In this study,a new pretreatment method and gas chromatography(GC)/nitrogen–phosphorus detector detection technique were used to characterize these two kinds of drugs in urine and blood samples.For the purpose of isolation of these trace drugs from the samples,liquid‑liquid extraction/solid‑phase extraction was modified and validated for this specific study.The pretreatment method presented in this paper has many advantages,such as high recovery rate,high extraction efficiency,high detection sensitivity,low limit of detection,and simple operation.The GC/NPD instrument is popular in most laboratories because it can meet the routine requirements of forensic science.All these aspects make this combination of sample pretreatment and GC/NPD technique the most suitable choice for drug detection in biological samples.
基金This work is one part of research on drug and development of explosive detection technique,and was supported by the Opening Project of Key Laboratory of Evidence Science(China University of Political Science and Law)Ministry of Education(2012KFKT07)+1 种基金the Program for Young Innovative Research Team in China University of Political Science and Law(1000-10814344)Academician Foundation of the Ministry of Public Security of the People's Republic of China(No.2011-23210044,2011-23211119,23212052).
文摘2,4,6-trinitrotoluene(TNT)is a commonly used explosive.It is not only a threat to public safety but also causes environmental pollution,affecting human health.However,at this stage of TNT detection,technology cannot meet the demands of the current situation.To acquire a new method devoted to the study of fast and quantitative detection of TNT.It combines the molecular imprinting technique(MIT)with surface plasmon resonance(SPR)technology for high sensitivity.In this study,a molecularly imprinted polymer(MIP)film for the detection of TNT was synthesized by heat in acetonitrile at 60°C,using the TNT imprinting molecule and azobisisobutyronitrile(AEBN)as initiators.In the present work,there are many factors that can influence the elution efficiency,such as raw material ratios,fore-reaction time,reaction time,etc.The polymers have the highest elution efficiency when raw material mole ratios[n(TNT):n methacrylic acid(MAA):n ethylene glycol dimethylacrylate(EGDMA)]were 1:4:8;the MIP sensor could detect a TNT concentration as low as 1x10-10 M.Compared to the blank polymer with the same chemical composition,the imprinted polymer had higher binding efficiency and higher selectivity.
基金Humanities and Social Science Research Program of China University of Politics and Law(10ZFQ82009)Academician Foundation of the Ministry of Public Security of the People’s Republic of China(No.2011‑23214203,2011‑23215243)+1 种基金Beijing Municipal Education Commission University Science and Technology Park Construction Project(2011‑08111603)Program for Young Innovative Research Team in China University of Political Science and Law(14CXTD04,16CXTD05),for their financial support.
文摘A gold nanoparticle‑enhanced surface plasmon resonance(SPR)aptasensor was developed for high‑specificity and high‑sensitivity detection of 2,4,6‑trinitrotoluene(TNT).Self‑assembly film‑forming technology was used to modify the gold surface of the sensor chip with 2,4,6‑trinitrophenyl glycine,a TNT analogue,using polyethylene glycol to which the thiol group and carboxyl group are attached.Aptamer‑gold nanoparticle complexes were formed through Au‑S bonding.To detect TNT,the samples were incubated with the aptamer‑gold nanoparticle complexes,and the solution competition method was applied through the SPR aptasensor.The results showed that the SPR aptasensor achieved fast,real‑time detection of TNT.This gold nanoparticle‑enhanced SPR aptasensor is suitable for TNT detection in the field of public safety and environmental monitoring.
