Astrocytes are the most abundant glial cells in the central nervous system;they participate in crucial biological processes,maintain brain structure,and regulate nervous system function.Exosomes are cell-derived extra...Astrocytes are the most abundant glial cells in the central nervous system;they participate in crucial biological processes,maintain brain structure,and regulate nervous system function.Exosomes are cell-derived extracellular vesicles containing various bioactive molecules including proteins,peptides,nucleotides,and lipids secreted from their cellular sources.Increasing evidence shows that exosomes participate in a communication network in the nervous system,in which astrocyte-derived exosomes play important roles.In this review,we have summarized the effects of exosomes targeting astrocytes and the astrocyte-derived exosomes targeting other cell types in the central nervous system.We also discuss the potential research directions of the exosome-based communication network in the nervous system.The exosome-based intercellular communication focused on astrocytes is of great significance to the biological and/or pathological processes in different conditions in the brain.New strategies may be developed for the diagnosis and treatment of neurological disorders by focusing on astrocytes as the central cells and utilizing exosomes as communication mediators.展开更多
Introduction:Among all malignant tumors of the digestive system,pancreatic carcinoma exhibits the highest mortality rate.Currently,prevention and effective treatment are urgent issues that need to be addressed.Methods...Introduction:Among all malignant tumors of the digestive system,pancreatic carcinoma exhibits the highest mortality rate.Currently,prevention and effective treatment are urgent issues that need to be addressed.Methods:The study focused on meiotic nuclear divisions 1(MND1),integrating data from the Gene Expression Profiling Interactive Analysis(GEPIA)database with prognostic survival analysis.Simultaneously,experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC.The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA(siRNA)in Patu-8988 and Panc1 cell lines.Results:The results of Cell Counting Kit-8 indicated that the suppression of MND1 resulted in a decrease in cell proliferation.Wound healing and Transwell assays revealed that MND1 knockdown reduced cell migration and invasion.Flow cytometry revealed that inhibiting MND1 hindered the cell cycle.Furthermore,MND1 could stimulate the proliferation,migration,and invasion of Patu-8988 and Panc1 cells by increasing the expression of MND1.Notably,MND1 had a positive effect on H2AFX expression in PC cells.Elevated MND1 expression suggests the low overall survival rate of individuals diagnosed with PC.Conclusion:These findings suggest that MND1 has the potential to be a gene with the ability to accurately diagnose and treat PC.展开更多
Two female-specific AFLP(amplified fragment length polymorphism)markers(named CseF464 and CseF136)were isolated by using one selective primer combination(E-AGC/M-CTG)from the genomic DNA of 20 females and 20 males of ...Two female-specific AFLP(amplified fragment length polymorphism)markers(named CseF464 and CseF136)were isolated by using one selective primer combination(E-AGC/M-CTG)from the genomic DNA of 20 females and 20 males of the half-smooth tongue sole Cynoglossus semilaevis.Both the markers were re-amplified,recovered from the agarose gels,cloned and sequenced.Bioinformatics analysis indicated that the length of the two markers were 468 bp and 134 bp,respectively,and the sequences showed no similarity to each other,as well as to the known sequences deposited in the GenBank database using BLASTn.Two pairs of SCAR(sequence characterized amplified regions)primers were designed based on the sequences of the two female-specific markers.Furthermore,PCR-based genetic sex identification method was developed in Cynoglossus semilaevis.A specific fragment was amplified in all females but not in any males by using these SCAR primers on the initial 20 female and 20 male individuals of Cynoglossus semilaevis.The feasibility of the two SCAR primer pairs was confirmed in additional 100 individuals(50 females and 50 males).This allowed for reliable,rapid molecular identification of genetic sex of the species,genetic mapping on the sex chromosomes and better understanding of the sex determination and sex differentiation in the half-smooth tongue sole.展开更多
An ultra-wideband metamaterial absorber is developed,which is polarized-insensitive and angular-stable.Three layers of square resistive films comprise the proposed metamaterial.The optimal values of geometric paramete...An ultra-wideband metamaterial absorber is developed,which is polarized-insensitive and angular-stable.Three layers of square resistive films comprise the proposed metamaterial.The optimal values of geometric parameters are obtained,such that the designed absorber can achieve an ultra-broadband absorption response from 4.73 to 39.04 GHz(relative bandwidth of 156.7%)for both transverse electricity and transverse magnetic waves.Moreover,impedance matching theory and an equivalent circuit model are utilized for the absorption mechanism analysis.The compatibility of equivalent circuit calculation results,together with both full-wave simulation and experimental results,demonstrates the excellent performance and applicability of the proposed metamaterial absorber.展开更多
Oceanic turbulence measurements made by an acoustic Doppler velocimeter(ADV)suffer from noise that potentially affects the estimates of turbulence statistics.This study examines the abilities of Kalman filtering and a...Oceanic turbulence measurements made by an acoustic Doppler velocimeter(ADV)suffer from noise that potentially affects the estimates of turbulence statistics.This study examines the abilities of Kalman filtering and autoregressive moving average models to eliminate noise in ADV velocity datasets of laboratory experiments and offshore observations.Results show that the two methods have similar performance in ADV de-noising,and both effectively reduce noise in ADV velocities,even in cases of high noise.They eliminate the noise floor at high frequencies of the velocity spectra,leading to a longer range that effectively fits the Kolmogorov-5/3 slope at midrange frequencies.After de-noising adopting the two methods,the values of the mean velocity are almost unchanged,while the root-mean-square horizontal velocities and thus turbulent kinetic energy decrease appreciably in these experiments.The Reynolds stress is also affected by high noise levels,and de-noising thus reduces uncertainties in estimating the Reynolds stress.展开更多
The slower response speed is the main problem in the application of ZnO quantum dots(QDs)photodetector,which has been commonly attributed to the presence of excess oxygen vacancy defects and oxygen adsorption/desorpti...The slower response speed is the main problem in the application of ZnO quantum dots(QDs)photodetector,which has been commonly attributed to the presence of excess oxygen vacancy defects and oxygen adsorption/desorption processes.However,the detailed mechanism is still not very clear.Herein,the properties of ZnO QDs and their photodetectors with different amounts of oxygen vacancy(VO)defects controlled by hydrogen peroxide(H_(2)O_(2))solution treatment have been investigated.After H_(2)O_(2) solution treatment,VO concentration of ZnO QDs decreased.The H_(2)O_(2) solution-treated device has a higher photocurrent and a lower dark current.Meanwhile,with the increase in VO concentration of ZnO QDs,the response speed of the device has been improved due to the increase of oxygen adsorption/desorption rate.More interestingly,the response speed of the device became less sensitive to temperature and oxygen concentration with the increase of VO defects.The findings in this work clarify that the surface VO defects of ZnO QDs could enhance the photoresponse speed,which is helpful for sensor designing.展开更多
At the end of May 2008,a massive bloom of macroalgae occurred in the western Yellow Sea off China and lasted for nearly two months,and annual blooms have occurred since then on. During bloom period,the surface-driftin...At the end of May 2008,a massive bloom of macroalgae occurred in the western Yellow Sea off China and lasted for nearly two months,and annual blooms have occurred since then on. During bloom period,the surface-drifting macroalgae have showed an interesting pattern dominated by a banded structure,and the distance between neighboring bands ranged from hundreds of meters to about 6 km with a peak at 1–1.5 km,which is an order of higher than the scale of Langmuir circulation of 50–100 m. In order to explain this new phenomenon,ocean current data obtained from a Doppler current profiler off Qingdao was used to implement stability analysis. By numerically solving the resulting differential Orr-Sommerfeld equation,the secondary circulation induced from the instability of the Emkan current was found to fit well with the observed spatial scale of the surface-drifting macroalgae’s banded structure. As the wind driven Emkan current exist universally in the global ocean,it is reasonable to conclude that the banded structure with kilometers distance between adjoining bands is ubiquitous. We found a new circulation in the upper ocean which is important for exchange of energy,materials and gas between the upper ocean and subsurface layer.展开更多
In order to see whether carbon ion (C-ion) beams have a biological advantage over X-rays, studies were designed to examine the effects of C-ion beams on radiosensitivity in X-ray resistant cells. Clinically relevant X...In order to see whether carbon ion (C-ion) beams have a biological advantage over X-rays, studies were designed to examine the effects of C-ion beams on radiosensitivity in X-ray resistant cells. Clinically relevant X-ray resistant SAS-R cells derived from human tongue cancer SAS cells were used. The cells were exposed to X-rays or Spread-Out Bragg peak (SOBP) beam C-ions. Cell survival was measured using a modified high-density survival assay. Cell survival signaling and cell death signaling were analyzed using flow cytometry. The cells were labeled with putative cancer stem cell markers such as CD44 and CD326. SAS-R cells were 1.6 times more radioresistant than SAS cells after exposure to X-rays. Cell survival was similar in each cell line after exposure to C-ion beams. SAS-R cells displayed enhanced cell survival signaling when compared to SAS cells under normal conditions. On the other hand, the phosphorylation of AKT-related proteins decreased and polycaspase activities were enhanced when cells were irradiated with C-ion beams in both cell lines. More CD44 and CD326 positive cells were seen in SAS-R cells than in SAS cells. Moreover, the marker positive cell numbers significantly decreased after exposure to C-ion beams when compared to X-rays at iso-survival doses in SAS-R cells. C-ion beams efficiently induced cell killing in X-ray resistant cells which displayed activated cell survival signaling and contained more numerous cancer stem-like cells.展开更多
This paper presents the development of a liquid pressure based automatic monitoring device of the vertical displacement of oil pipeline that has good applicability to the extreme cold conditions of the permafrost regi...This paper presents the development of a liquid pressure based automatic monitoring device of the vertical displacement of oil pipeline that has good applicability to the extreme cold conditions of the permafrost region and is capable of automatic collection and transmission of monitoring data. The effectiveness of the device has been verified using total station measurement method and the results show that the device can intuitively indicate the vertical displacement of the pipe under the harmful influence of frost heaving and thaw settlement. According to the monitoring data, safety evaluation of the pipe during the monitoring period is carried out using stress-based design criteria and strain-based design criteria respectively. The analytic results with stress-based design criteria show that the pipe may be in a dangerous state while the results with strain-based design criteria show that the pipe is in a safe state. The design method with strain-based criteria is complementary to the design method with stress-based criteria. Separate use of either method is not recommended.展开更多
Aluminum hydride(AlH3) is a binary metal hydride that contains more than 10.1 wt% of hydrogen and possesses a high volumetric hydrogen density of 148 kg H2 m^(-3).Pristine AlH3 can readily release hydrogen at a modera...Aluminum hydride(AlH3) is a binary metal hydride that contains more than 10.1 wt% of hydrogen and possesses a high volumetric hydrogen density of 148 kg H2 m^(-3).Pristine AlH3 can readily release hydrogen at a moderate temperature below 200℃.Such high hydrogen density and low desorption temperature make AlH3 one of most promising hydrogen storage media for mobile application.This review covers the research activity on the structures,synthesis,decomposition thermodynamics and kinetics,regeneration and application validation of AlH3 over the past decades.Finally,the future research directions of AlH3 as a hydrogen storage material will be revealed.展开更多
The mud crab Scylla paramamosain is a key species in China due to its high nutritional value and great economic worth and has grown in popularity.Myo-inositol can modulate versatile physiological functions in aquatic ...The mud crab Scylla paramamosain is a key species in China due to its high nutritional value and great economic worth and has grown in popularity.Myo-inositol can modulate versatile physiological functions in aquatic animals.In the present study,S.paramamosain megalopa were given graded concentrations of myo-inositol(0,1,2,4,and 8 ppm)by water immersion to explore how their metamorphosis would be affected.The results showed that supplementing with myo-inositol remarkably increased transformation and survival rate from megalopa to crablet by at least 1.16 and 1.26 times,respectively.To decipher the molecular mechanism of how myo-inositol increases metamorphosis and survival rate,we further performed transcriptome-based gene expression profiling of both megalopa and crablet treated with myo-inositol.The integrative transcriptome analyses predicted that the differentially expressed genes(DEGs)were significantly enriched in chitinase activity,structural constituent of cuticle,and chitin binding,which are associated with the decomposition and reconstruction of cuticle.qPCR results confirmed that myo-inositol mediated gene expression levels of the above cuticle-related pathways.Considering the importance of the cuticle in exoskeleton formation and molting,it can be concluded that myo-inositol-induced changes in the cuticle decomposition and reconstruction might have accelerated the transformation from megalopa to crablet of S.paramamosain.Besides,numerous DEGs were significantly enriched in protein digestion and absorption,amino sugar and nucleotide sugar metabolism.It implies that myo-inositol may improve survival by regulating energy or nutrient absorption.Additionally,the accelerated metamorphosis by myo-inositol may improve survival from megalopa to crablet of S.paramamosain.Overall,this study will provide the first insights into the underlying molecular mechanisms by which myo-inositol increases metamorphosis and survival.展开更多
The chloroplast is a critical battleground in the arms race between plants and pathogens.Among microbe-secreted mycotoxins,tenuazonic acid(TeA),produced by the genus Alternaria and other phytopathogenic fungi,inhibits...The chloroplast is a critical battleground in the arms race between plants and pathogens.Among microbe-secreted mycotoxins,tenuazonic acid(TeA),produced by the genus Alternaria and other phytopathogenic fungi,inhibits photosynthesis,leading to a burst of photosynthetic singlet oxygen(1O2)that is implicated in damage and chloroplast-to-nucleus retrograde signaling.Despite the signicant crop damage caused by Alternaria pathogens,our understanding of the molecular mechanism by which TeA promotes pathoge-nicity and cognate plant defense responses remains fragmentary.We now reveal that A.alternata induces necrotrophic foliar lesions by harnessing EXECUTER1(EX1)/EX2-mediated chloroplast-to-nucleus retro-grade signaling activated by TeA toxin–derived photosynthetic 1O2 in Arabidopsis thaliana.Mutation of the 1O2-sensitive EX1-W643 residue or complete deletion of the EX1 singlet oxygen sensor domain compro-mises expression of 1O2-responsive nuclear genes and foliar lesions.We also found that TeA toxin rapidly induces nuclear genes implicated in jasmonic acid(JA)synthesis and signaling,and EX1-mediated retro-grade signaling appears to be critical for establishing a signaling cascade from 1O2 to JA.The present study sheds new light on the foliar pathogenicity of A.alternata,during which EX1-dependent 1O2 signaling in-duces JA-dependent foliar cell death.展开更多
Holography can provide the desired wavefront phase and/or amplitude for imaging,particle manipulation,bacteria trapping,and cell patterning in optics and acoustics.However,previous work on acoustic holography is mostl...Holography can provide the desired wavefront phase and/or amplitude for imaging,particle manipulation,bacteria trapping,and cell patterning in optics and acoustics.However,previous work on acoustic holography is mostly based on local design optimization,either using active control of the sound source or relying on the structural design to provide the desired wavefront.Achieving precise control over the acoustic field remains a significant challenge.Here,we realize refined single-plane symmetric binary amplitude,asymmetric intensity gradient amplitude,and bi-objective hologram through the non-local holographic imaging theory that considers the acoustic coupling of structural units in detail.By taking into account the self-radiation and mutual radiation between many small units on a plate of well-designed thickness,as well as the transmission through the plate's apertures,we can effectively regulate the sound field behind the plate.We demonstrate the effectiveness of our approach through numerical simulations and experiments,showcasing a circle,a black hole,and a bi-objective with a circle and a square hologram.Notably,the acoustic black hole hologram precisely reconstructs the intensity gradient distribution at two bright spots.This non-local holographic imaging theory is valuable for the fine-intensity regulation of the sound field and is expected to be applied in ultrasound diagnosis and treatment,medical imaging,and other fields.展开更多
Graphene based nanosheets have been widely used as building blocks for fabrication of superior separation membrane for water processing.In particular,membranes made of reduced graphene oxide(rGO)show better stability ...Graphene based nanosheets have been widely used as building blocks for fabrication of superior separation membrane for water processing.In particular,membranes made of reduced graphene oxide(rGO)show better stability compared with graphene oxide(GO).However,densely stacked of rGO often results in low water flux.In this study,cellulose nanofibers(CNFs)were incorporated into the rGO laminates by vacuum filtration of dilute GO/CNF solution and thermal reduction at 150C for 1.5 h.The resulting rGO/CNF membrane was treated with oxygen plasma for 1–4 min to create nanopores on the membrane surface for the purpose of enhancing nano-filtration performance.The results showed that the optimum membrane performance was obtained by using the equal amount of GO(31.83 mg m^(-2))and CNFs accompanied by 3 min of plasma treatment,exhibiting a pure water permeance of 37.23.9 L m^(-2)h^(-1)bar^(-1)maintaining a rejection above 90%for Acid Fuchsin(1.2×1.1 nm),Rose Bengal(1.5×1.2 nm)and Brilliant Blue(2.2×1.7 nm).展开更多
Gynogenesis is an established technique to generate all female type offspring and this technique has been successfully induced diploid gynogens progeny in aquatic animals of fishes and crustaceans.Monosex culture of a...Gynogenesis is an established technique to generate all female type offspring and this technique has been successfully induced diploid gynogens progeny in aquatic animals of fishes and crustaceans.Monosex culture of all female shrimp and fishes were selected attribute to all female type offspring which have better size than male and help increase the market size and profitable.This article discusses on the protocol applied to produce gynogens progeny and the successful rate of gynogenesis production in fishes,molluscs and aquatic crustaceans of shrimps in general.Overall most of the UV length applied for irradiated the sperm were around 254-365 nm for(20-40 s),(20-80 s)and(5-8 s)for shrimps,254 nm,30 s for molluscs species and for fishes were around 254 nm for(1.5min)and(2-12 min)time of exposure respectively.For gynogenesis induction,the fertilized eggs were treated with cold shock,heat shock or cytochalasin-B for both shrimp and fishes gynogens technique.Fertilization rate was identified around 4.33%-19.67%in shrimp.Successful hatching rate was identified around 3.0%,14.9%-37.2%of gynogens offspring in shrimp and various percentages of hatching rates were identified from each species of fish gynogens.Overall,there is still low survival rate of gynogens produced using gynogenesis technique and further study should be carried out to improve the gynogens production.The discussed protocols serve as a guide lines for the gynogenesis technique application of all female monosex culture in the future.展开更多
L-Ergothioneine(L-EGT)possesses excellent antioxidant activity and has been used in the food,pharmaceuticals and cosmetics industries.In this study,a new efficient and sensitive ultra-performance liquid chromatography...L-Ergothioneine(L-EGT)possesses excellent antioxidant activity and has been used in the food,pharmaceuticals and cosmetics industries.In this study,a new efficient and sensitive ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method was established for the quantitative determination of L-EGT in food.The sample was extracted with methanol-water(70:30,V/V),separated by hydrophilic interaction liquid chromatography(HILIC)and detected by triple-quadrupole mass spectrometry.Validation studies were carried out on different product and the limit of quantitation was 20μg/kg(milk,alcohol-free beverages,dairy products)and 40µg/kg(cereal bars,chocolate).Excellent linearity(correlation coefficient(R2)≥0.999)was achieved for L-EGT quantification in the range of 5–200 ng/mL.The recoveries of the method(83.7%−107.5%)and the relative standard deviation(RSD,0.88%−6.84%(n=6))meet the performance criteria required for the determination of L-EGT in food.Finally,the applicability of the method was tested by analysing actual samples.In general,the method developed is simple,reliable,accurate,and stable and could be useful for routine analyses of L-EGT in food.展开更多
To obtain a high-performance heterogeneous photo-catalyst, herein, the hetero-structured Zn In_(2)S_(4)-Ni O@MOF(ZNM) nano-sheets are designed and prepared by partial pyrolysis of nickel-based MOFs(NiMOF) combined wit...To obtain a high-performance heterogeneous photo-catalyst, herein, the hetero-structured Zn In_(2)S_(4)-Ni O@MOF(ZNM) nano-sheets are designed and prepared by partial pyrolysis of nickel-based MOFs(NiMOF) combined with the low-temperature solvo-thermal method. The results indicate that the NiO nanoparticles, produced by partial pyrolysis of the Ni-MOF, have a high density of the surface active sites with limited aggregation, which act as a co-catalyst to capture photo-induced charge carriers. In addition, the morphology and structure of Ni-MOF nano-sheets were preserved in ZNM, which is beneficial to the reduction of the conduction barrier for the photo generated electron-hole pairs. With the synergetic advantages of co-catalyst and unique two-dimensional hetero-structure, ZNM nano-sheets exhibited significantly improved activity for photo-catalytic hydrogen production.展开更多
Background:Cerebrovascular disease has become the leading cause of death in China.The purpose of this article is to analyze China’s contribution to the interventional treatment of cerebrovascular diseases.Methods:Bib...Background:Cerebrovascular disease has become the leading cause of death in China.The purpose of this article is to analyze China’s contribution to the interventional treatment of cerebrovascular diseases.Methods:Bibliometric analysis was used for evaluating the quantity,quality,research hotspots,and cooperation network of publications regarding interventional treatment of cerebrovascular diseases from China.These articles were searched from the database of Web of Science Core Collection.The authors,publication years,citation times,regions,and source journals of retrieved articles were recorded.Network analysis and visualization were performed on Citespace5.6.Results:From 1991 to 2019,a total of 5052 articles regarding cerebrovascular intervention were contributed by Chinese researchers.The number of publications from China grew fastest annually in the latest 5 years among countries.These publications were cited 61,216 times,with 12.12 average citations per item.The h-index was 82.Affiliated hospitals of Capital Medical University contributed most articles.Cerebral ischemia and intracranial aneurysm were the most popular keywords over the three decades.The timeline view of keywords indicated that cerebral ischemia always was a hot spot.Stent techniques were the main treatment tools and still had a strong developing trend.Neural regeneration and neuroprotection were the hot topics of basic researches related to cerebrovascular intervention.Conclusions:The number of researches grows rapidly in China over the decades,but the quality still needs further improvement.The increasing contributions of Chinese researchers to the global knowledge system of cerebrovascular intervention are promising.展开更多
基金supported by the National Natural Science Foundation of China,No.82071278(to PY)Outstanding Young Medical Talents Project of Changhai Hospital,No.2021JCSQ03(to PY)+1 种基金Shanghai Sailing Program,No.20YF1448000(to XZ)Medical Health Science and Technology Project of Zhoushan City,No.2022JRC01(to HL).
文摘Astrocytes are the most abundant glial cells in the central nervous system;they participate in crucial biological processes,maintain brain structure,and regulate nervous system function.Exosomes are cell-derived extracellular vesicles containing various bioactive molecules including proteins,peptides,nucleotides,and lipids secreted from their cellular sources.Increasing evidence shows that exosomes participate in a communication network in the nervous system,in which astrocyte-derived exosomes play important roles.In this review,we have summarized the effects of exosomes targeting astrocytes and the astrocyte-derived exosomes targeting other cell types in the central nervous system.We also discuss the potential research directions of the exosome-based communication network in the nervous system.The exosome-based intercellular communication focused on astrocytes is of great significance to the biological and/or pathological processes in different conditions in the brain.New strategies may be developed for the diagnosis and treatment of neurological disorders by focusing on astrocytes as the central cells and utilizing exosomes as communication mediators.
基金supported by grants from National Innovation Program for College Students(202210367076)Graduate Student Research Innovation Program of Bengbu Medical College(Byycxz22016)the National Natural Science Foundation of China(82072585),and the Key Research Project of Bengbu Medical College(No.2020byzd029).
文摘Introduction:Among all malignant tumors of the digestive system,pancreatic carcinoma exhibits the highest mortality rate.Currently,prevention and effective treatment are urgent issues that need to be addressed.Methods:The study focused on meiotic nuclear divisions 1(MND1),integrating data from the Gene Expression Profiling Interactive Analysis(GEPIA)database with prognostic survival analysis.Simultaneously,experiments at cellular level were employed to demonstrate the effect of MND1 on the proliferation and migration of PC.The small-molecule inhibitor of MND1 was used to suppress the migration of PC cells by knocking down MND1 using small interfering RNA(siRNA)in Patu-8988 and Panc1 cell lines.Results:The results of Cell Counting Kit-8 indicated that the suppression of MND1 resulted in a decrease in cell proliferation.Wound healing and Transwell assays revealed that MND1 knockdown reduced cell migration and invasion.Flow cytometry revealed that inhibiting MND1 hindered the cell cycle.Furthermore,MND1 could stimulate the proliferation,migration,and invasion of Patu-8988 and Panc1 cells by increasing the expression of MND1.Notably,MND1 had a positive effect on H2AFX expression in PC cells.Elevated MND1 expression suggests the low overall survival rate of individuals diagnosed with PC.Conclusion:These findings suggest that MND1 has the potential to be a gene with the ability to accurately diagnose and treat PC.
基金supported by grants from State High-Technology R&D Project of China(863)(2006AA10A403)Shandong Genetic Improvement Key Project for Agricultural Organism,and Taishan Scholar Project of Shandong Province
文摘Two female-specific AFLP(amplified fragment length polymorphism)markers(named CseF464 and CseF136)were isolated by using one selective primer combination(E-AGC/M-CTG)from the genomic DNA of 20 females and 20 males of the half-smooth tongue sole Cynoglossus semilaevis.Both the markers were re-amplified,recovered from the agarose gels,cloned and sequenced.Bioinformatics analysis indicated that the length of the two markers were 468 bp and 134 bp,respectively,and the sequences showed no similarity to each other,as well as to the known sequences deposited in the GenBank database using BLASTn.Two pairs of SCAR(sequence characterized amplified regions)primers were designed based on the sequences of the two female-specific markers.Furthermore,PCR-based genetic sex identification method was developed in Cynoglossus semilaevis.A specific fragment was amplified in all females but not in any males by using these SCAR primers on the initial 20 female and 20 male individuals of Cynoglossus semilaevis.The feasibility of the two SCAR primer pairs was confirmed in additional 100 individuals(50 females and 50 males).This allowed for reliable,rapid molecular identification of genetic sex of the species,genetic mapping on the sex chromosomes and better understanding of the sex determination and sex differentiation in the half-smooth tongue sole.
基金Supported by the Six Talent Peaks Project in Jiangsu Province(Grant No.XYDXX-072)the National Natural Science Foundation of China(Grant Nos.61372048 and 61771226)the Natural Science Foundation of Jiangsu Province(Grant No.BK20161186).
文摘An ultra-wideband metamaterial absorber is developed,which is polarized-insensitive and angular-stable.Three layers of square resistive films comprise the proposed metamaterial.The optimal values of geometric parameters are obtained,such that the designed absorber can achieve an ultra-broadband absorption response from 4.73 to 39.04 GHz(relative bandwidth of 156.7%)for both transverse electricity and transverse magnetic waves.Moreover,impedance matching theory and an equivalent circuit model are utilized for the absorption mechanism analysis.The compatibility of equivalent circuit calculation results,together with both full-wave simulation and experimental results,demonstrates the excellent performance and applicability of the proposed metamaterial absorber.
基金The National Key Research and Development Program of China under contract No.2017YFC1404000the Basic Scientific Fund for National Public Research Institutes of China under contract No.2018S03the National Natural Science Foundation of China under contract Nos 41776038 and 41821004
文摘Oceanic turbulence measurements made by an acoustic Doppler velocimeter(ADV)suffer from noise that potentially affects the estimates of turbulence statistics.This study examines the abilities of Kalman filtering and autoregressive moving average models to eliminate noise in ADV velocity datasets of laboratory experiments and offshore observations.Results show that the two methods have similar performance in ADV de-noising,and both effectively reduce noise in ADV velocities,even in cases of high noise.They eliminate the noise floor at high frequencies of the velocity spectra,leading to a longer range that effectively fits the Kolmogorov-5/3 slope at midrange frequencies.After de-noising adopting the two methods,the values of the mean velocity are almost unchanged,while the root-mean-square horizontal velocities and thus turbulent kinetic energy decrease appreciably in these experiments.The Reynolds stress is also affected by high noise levels,and de-noising thus reduces uncertainties in estimating the Reynolds stress.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.62074148,61875194,11727902,12074372,11774341,11974344,61975204,and 11804335)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Grant No.2020225)the Open Project of the State Key Laboratory of Luminescence and Applications(Grant Nos.SKLA-2020-02 and SKLA-2020-06).
文摘The slower response speed is the main problem in the application of ZnO quantum dots(QDs)photodetector,which has been commonly attributed to the presence of excess oxygen vacancy defects and oxygen adsorption/desorption processes.However,the detailed mechanism is still not very clear.Herein,the properties of ZnO QDs and their photodetectors with different amounts of oxygen vacancy(VO)defects controlled by hydrogen peroxide(H_(2)O_(2))solution treatment have been investigated.After H_(2)O_(2) solution treatment,VO concentration of ZnO QDs decreased.The H_(2)O_(2) solution-treated device has a higher photocurrent and a lower dark current.Meanwhile,with the increase in VO concentration of ZnO QDs,the response speed of the device has been improved due to the increase of oxygen adsorption/desorption rate.More interestingly,the response speed of the device became less sensitive to temperature and oxygen concentration with the increase of VO defects.The findings in this work clarify that the surface VO defects of ZnO QDs could enhance the photoresponse speed,which is helpful for sensor designing.
基金The National Natural Science Foundation of China under contract No.41821004the National Program on Global Change and Air-Sea Interaction under contract No.GASI-IPOVAI-05.
文摘At the end of May 2008,a massive bloom of macroalgae occurred in the western Yellow Sea off China and lasted for nearly two months,and annual blooms have occurred since then on. During bloom period,the surface-drifting macroalgae have showed an interesting pattern dominated by a banded structure,and the distance between neighboring bands ranged from hundreds of meters to about 6 km with a peak at 1–1.5 km,which is an order of higher than the scale of Langmuir circulation of 50–100 m. In order to explain this new phenomenon,ocean current data obtained from a Doppler current profiler off Qingdao was used to implement stability analysis. By numerically solving the resulting differential Orr-Sommerfeld equation,the secondary circulation induced from the instability of the Emkan current was found to fit well with the observed spatial scale of the surface-drifting macroalgae’s banded structure. As the wind driven Emkan current exist universally in the global ocean,it is reasonable to conclude that the banded structure with kilometers distance between adjoining bands is ubiquitous. We found a new circulation in the upper ocean which is important for exchange of energy,materials and gas between the upper ocean and subsurface layer.
文摘In order to see whether carbon ion (C-ion) beams have a biological advantage over X-rays, studies were designed to examine the effects of C-ion beams on radiosensitivity in X-ray resistant cells. Clinically relevant X-ray resistant SAS-R cells derived from human tongue cancer SAS cells were used. The cells were exposed to X-rays or Spread-Out Bragg peak (SOBP) beam C-ions. Cell survival was measured using a modified high-density survival assay. Cell survival signaling and cell death signaling were analyzed using flow cytometry. The cells were labeled with putative cancer stem cell markers such as CD44 and CD326. SAS-R cells were 1.6 times more radioresistant than SAS cells after exposure to X-rays. Cell survival was similar in each cell line after exposure to C-ion beams. SAS-R cells displayed enhanced cell survival signaling when compared to SAS cells under normal conditions. On the other hand, the phosphorylation of AKT-related proteins decreased and polycaspase activities were enhanced when cells were irradiated with C-ion beams in both cell lines. More CD44 and CD326 positive cells were seen in SAS-R cells than in SAS cells. Moreover, the marker positive cell numbers significantly decreased after exposure to C-ion beams when compared to X-rays at iso-survival doses in SAS-R cells. C-ion beams efficiently induced cell killing in X-ray resistant cells which displayed activated cell survival signaling and contained more numerous cancer stem-like cells.
文摘This paper presents the development of a liquid pressure based automatic monitoring device of the vertical displacement of oil pipeline that has good applicability to the extreme cold conditions of the permafrost region and is capable of automatic collection and transmission of monitoring data. The effectiveness of the device has been verified using total station measurement method and the results show that the device can intuitively indicate the vertical displacement of the pipe under the harmful influence of frost heaving and thaw settlement. According to the monitoring data, safety evaluation of the pipe during the monitoring period is carried out using stress-based design criteria and strain-based design criteria respectively. The analytic results with stress-based design criteria show that the pipe may be in a dangerous state while the results with strain-based design criteria show that the pipe is in a safe state. The design method with strain-based criteria is complementary to the design method with stress-based criteria. Separate use of either method is not recommended.
基金financially supported by the National Natural Science Foundation of China (No. 51771171, and 51971199)Education Department of Guangxi Zhuang Autonomous Region (No. 2019KY0021)the Natural Science Foundation of Guangxi Province (2019GXNSFBA185004, and 2018GXNSFAA281308)。
文摘Aluminum hydride(AlH3) is a binary metal hydride that contains more than 10.1 wt% of hydrogen and possesses a high volumetric hydrogen density of 148 kg H2 m^(-3).Pristine AlH3 can readily release hydrogen at a moderate temperature below 200℃.Such high hydrogen density and low desorption temperature make AlH3 one of most promising hydrogen storage media for mobile application.This review covers the research activity on the structures,synthesis,decomposition thermodynamics and kinetics,regeneration and application validation of AlH3 over the past decades.Finally,the future research directions of AlH3 as a hydrogen storage material will be revealed.
基金the Leading Talent Project of Special Support Plan of Guangdong Province[grant number 2019TX05N067]the special Projects in Key Fields of Colleges and Universities in Guangdong Province[grant numbers 2020ZDZX1001]+2 种基金the National Natural Science Foundation of China[grant numbers 42076133,42206127]the Science and Technology Project of Guangdong Province[grant number STKJ202209029,KTP20210376]the Guangdong Basic and Applied Basic Research Fund Regional Joint Fund-Youth Fund Project[grant number 2021A1515110514].
文摘The mud crab Scylla paramamosain is a key species in China due to its high nutritional value and great economic worth and has grown in popularity.Myo-inositol can modulate versatile physiological functions in aquatic animals.In the present study,S.paramamosain megalopa were given graded concentrations of myo-inositol(0,1,2,4,and 8 ppm)by water immersion to explore how their metamorphosis would be affected.The results showed that supplementing with myo-inositol remarkably increased transformation and survival rate from megalopa to crablet by at least 1.16 and 1.26 times,respectively.To decipher the molecular mechanism of how myo-inositol increases metamorphosis and survival rate,we further performed transcriptome-based gene expression profiling of both megalopa and crablet treated with myo-inositol.The integrative transcriptome analyses predicted that the differentially expressed genes(DEGs)were significantly enriched in chitinase activity,structural constituent of cuticle,and chitin binding,which are associated with the decomposition and reconstruction of cuticle.qPCR results confirmed that myo-inositol mediated gene expression levels of the above cuticle-related pathways.Considering the importance of the cuticle in exoskeleton formation and molting,it can be concluded that myo-inositol-induced changes in the cuticle decomposition and reconstruction might have accelerated the transformation from megalopa to crablet of S.paramamosain.Besides,numerous DEGs were significantly enriched in protein digestion and absorption,amino sugar and nucleotide sugar metabolism.It implies that myo-inositol may improve survival by regulating energy or nutrient absorption.Additionally,the accelerated metamorphosis by myo-inositol may improve survival from megalopa to crablet of S.paramamosain.Overall,this study will provide the first insights into the underlying molecular mechanisms by which myo-inositol increases metamorphosis and survival.
基金funded by the National Key R&D Program (2021YFD1700100,2017YFD0201304)the Jiangsu Agricultural Science and Technology Innovation Fund (CX (21)3093)to S.C.+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB27040102)the National Natural Science Foundation of China (NSFC) (31871397)to C.K.
文摘The chloroplast is a critical battleground in the arms race between plants and pathogens.Among microbe-secreted mycotoxins,tenuazonic acid(TeA),produced by the genus Alternaria and other phytopathogenic fungi,inhibits photosynthesis,leading to a burst of photosynthetic singlet oxygen(1O2)that is implicated in damage and chloroplast-to-nucleus retrograde signaling.Despite the signicant crop damage caused by Alternaria pathogens,our understanding of the molecular mechanism by which TeA promotes pathoge-nicity and cognate plant defense responses remains fragmentary.We now reveal that A.alternata induces necrotrophic foliar lesions by harnessing EXECUTER1(EX1)/EX2-mediated chloroplast-to-nucleus retro-grade signaling activated by TeA toxin–derived photosynthetic 1O2 in Arabidopsis thaliana.Mutation of the 1O2-sensitive EX1-W643 residue or complete deletion of the EX1 singlet oxygen sensor domain compro-mises expression of 1O2-responsive nuclear genes and foliar lesions.We also found that TeA toxin rapidly induces nuclear genes implicated in jasmonic acid(JA)synthesis and signaling,and EX1-mediated retro-grade signaling appears to be critical for establishing a signaling cascade from 1O2 to JA.The present study sheds new light on the foliar pathogenicity of A.alternata,during which EX1-dependent 1O2 signaling in-duces JA-dependent foliar cell death.
基金supported by the National Key R&D Program of China(Grant Nos.2020YFA0211400,and 2020YFA0211402)the National Natural Science Foundation of China(Grant Nos.42074179,11774266,92263208,and 12074286)the Shanghai Science and Technology Committee(Grant No.21JC1405600)。
文摘Holography can provide the desired wavefront phase and/or amplitude for imaging,particle manipulation,bacteria trapping,and cell patterning in optics and acoustics.However,previous work on acoustic holography is mostly based on local design optimization,either using active control of the sound source or relying on the structural design to provide the desired wavefront.Achieving precise control over the acoustic field remains a significant challenge.Here,we realize refined single-plane symmetric binary amplitude,asymmetric intensity gradient amplitude,and bi-objective hologram through the non-local holographic imaging theory that considers the acoustic coupling of structural units in detail.By taking into account the self-radiation and mutual radiation between many small units on a plate of well-designed thickness,as well as the transmission through the plate's apertures,we can effectively regulate the sound field behind the plate.We demonstrate the effectiveness of our approach through numerical simulations and experiments,showcasing a circle,a black hole,and a bi-objective with a circle and a square hologram.Notably,the acoustic black hole hologram precisely reconstructs the intensity gradient distribution at two bright spots.This non-local holographic imaging theory is valuable for the fine-intensity regulation of the sound field and is expected to be applied in ultrasound diagnosis and treatment,medical imaging,and other fields.
基金the Australian Research Council(Project No.IH170100009)。
文摘Graphene based nanosheets have been widely used as building blocks for fabrication of superior separation membrane for water processing.In particular,membranes made of reduced graphene oxide(rGO)show better stability compared with graphene oxide(GO).However,densely stacked of rGO often results in low water flux.In this study,cellulose nanofibers(CNFs)were incorporated into the rGO laminates by vacuum filtration of dilute GO/CNF solution and thermal reduction at 150C for 1.5 h.The resulting rGO/CNF membrane was treated with oxygen plasma for 1–4 min to create nanopores on the membrane surface for the purpose of enhancing nano-filtration performance.The results showed that the optimum membrane performance was obtained by using the equal amount of GO(31.83 mg m^(-2))and CNFs accompanied by 3 min of plasma treatment,exhibiting a pure water permeance of 37.23.9 L m^(-2)h^(-1)bar^(-1)maintaining a rejection above 90%for Acid Fuchsin(1.2×1.1 nm),Rose Bengal(1.5×1.2 nm)and Brilliant Blue(2.2×1.7 nm).
文摘Gynogenesis is an established technique to generate all female type offspring and this technique has been successfully induced diploid gynogens progeny in aquatic animals of fishes and crustaceans.Monosex culture of all female shrimp and fishes were selected attribute to all female type offspring which have better size than male and help increase the market size and profitable.This article discusses on the protocol applied to produce gynogens progeny and the successful rate of gynogenesis production in fishes,molluscs and aquatic crustaceans of shrimps in general.Overall most of the UV length applied for irradiated the sperm were around 254-365 nm for(20-40 s),(20-80 s)and(5-8 s)for shrimps,254 nm,30 s for molluscs species and for fishes were around 254 nm for(1.5min)and(2-12 min)time of exposure respectively.For gynogenesis induction,the fertilized eggs were treated with cold shock,heat shock or cytochalasin-B for both shrimp and fishes gynogens technique.Fertilization rate was identified around 4.33%-19.67%in shrimp.Successful hatching rate was identified around 3.0%,14.9%-37.2%of gynogens offspring in shrimp and various percentages of hatching rates were identified from each species of fish gynogens.Overall,there is still low survival rate of gynogens produced using gynogenesis technique and further study should be carried out to improve the gynogens production.The discussed protocols serve as a guide lines for the gynogenesis technique application of all female monosex culture in the future.
基金This work was supported by National Key Research and Development Program of China(2019YFC1606400).
文摘L-Ergothioneine(L-EGT)possesses excellent antioxidant activity and has been used in the food,pharmaceuticals and cosmetics industries.In this study,a new efficient and sensitive ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method was established for the quantitative determination of L-EGT in food.The sample was extracted with methanol-water(70:30,V/V),separated by hydrophilic interaction liquid chromatography(HILIC)and detected by triple-quadrupole mass spectrometry.Validation studies were carried out on different product and the limit of quantitation was 20μg/kg(milk,alcohol-free beverages,dairy products)and 40µg/kg(cereal bars,chocolate).Excellent linearity(correlation coefficient(R2)≥0.999)was achieved for L-EGT quantification in the range of 5–200 ng/mL.The recoveries of the method(83.7%−107.5%)and the relative standard deviation(RSD,0.88%−6.84%(n=6))meet the performance criteria required for the determination of L-EGT in food.Finally,the applicability of the method was tested by analysing actual samples.In general,the method developed is simple,reliable,accurate,and stable and could be useful for routine analyses of L-EGT in food.
基金support of National Science Foundation of China (Nos.91963207 and 12075174)。
文摘To obtain a high-performance heterogeneous photo-catalyst, herein, the hetero-structured Zn In_(2)S_(4)-Ni O@MOF(ZNM) nano-sheets are designed and prepared by partial pyrolysis of nickel-based MOFs(NiMOF) combined with the low-temperature solvo-thermal method. The results indicate that the NiO nanoparticles, produced by partial pyrolysis of the Ni-MOF, have a high density of the surface active sites with limited aggregation, which act as a co-catalyst to capture photo-induced charge carriers. In addition, the morphology and structure of Ni-MOF nano-sheets were preserved in ZNM, which is beneficial to the reduction of the conduction barrier for the photo generated electron-hole pairs. With the synergetic advantages of co-catalyst and unique two-dimensional hetero-structure, ZNM nano-sheets exhibited significantly improved activity for photo-catalytic hydrogen production.
基金This work was supported by the National Natural Science Foundation of China(No 81870931)the Training Plan of Excellent Talents in Shanghai Municipality Health System(2017YQ034)
文摘Background:Cerebrovascular disease has become the leading cause of death in China.The purpose of this article is to analyze China’s contribution to the interventional treatment of cerebrovascular diseases.Methods:Bibliometric analysis was used for evaluating the quantity,quality,research hotspots,and cooperation network of publications regarding interventional treatment of cerebrovascular diseases from China.These articles were searched from the database of Web of Science Core Collection.The authors,publication years,citation times,regions,and source journals of retrieved articles were recorded.Network analysis and visualization were performed on Citespace5.6.Results:From 1991 to 2019,a total of 5052 articles regarding cerebrovascular intervention were contributed by Chinese researchers.The number of publications from China grew fastest annually in the latest 5 years among countries.These publications were cited 61,216 times,with 12.12 average citations per item.The h-index was 82.Affiliated hospitals of Capital Medical University contributed most articles.Cerebral ischemia and intracranial aneurysm were the most popular keywords over the three decades.The timeline view of keywords indicated that cerebral ischemia always was a hot spot.Stent techniques were the main treatment tools and still had a strong developing trend.Neural regeneration and neuroprotection were the hot topics of basic researches related to cerebrovascular intervention.Conclusions:The number of researches grows rapidly in China over the decades,but the quality still needs further improvement.The increasing contributions of Chinese researchers to the global knowledge system of cerebrovascular intervention are promising.
