Objective:To investigate the effect of Maxingganshi decoction on the expression of STAT4 and STAT6 in lung tissues of rats with chronic obstructive pulmonary disease (COPD).Methods:A total of 30 COPD Wistar rat models...Objective:To investigate the effect of Maxingganshi decoction on the expression of STAT4 and STAT6 in lung tissues of rats with chronic obstructive pulmonary disease (COPD).Methods:A total of 30 COPD Wistar rat models with phlegm-heat depression and lung depression were established, with half male and half female. The 30 model rats were fed in different cages, weighed and labeled. They were randomly divided into three groups: Maxingganshi decoction group, roxithromycin tablets control group, model control group. At the same time, 10 normal rats were selected as a blank control group. The white blood cell count and other cell count levels in the alveolar lavage fluid of the rats in the four groups, as well as the STAT4 and STAT6 protein levels in the lung tissues, were observed and compared.Results: After treatment, the white cell counts in Maxingganshi decoction group were significantly higher than that of the model control group (P<0.05);lymph, neutral particles and eosinophil levels were significantly lower than those of the model control group (P<0.05). Compared with roxithromycin tablets control group, white blood cell count and other classification level of cell count in Maxingganshi decoction group were not significantly different (P>0.05). After treatment, STAT4 protein levels in roxithromycin tablets control group and Maxingganshi decoction group were lower than that in the model control group (P<0.05), and STAT6 protein levels in roxithromycin tablets control group and Maxingganshi decoction group were higher than that of the model control group (P<0.05), suggesting the two means of intervention in this study could inhabit the STAT4 protein expression in lung tissue of COPD rats and promote effect on STAT6 protein. In addition, the level of STAT4 and STAT6 in the Maxingganshi decoction group was not significantly different from that in the roxithromycin tablets control group (P>0.05), suggesting that the effect of Maxingganshi decoction was similar to that of roxithromycin tablets.Conclusions: The action mechanism of Maxingganshi decoction group treating COPD may be through the STAT4 and STAT6 protein expression level to impose an effect, and thus interfere with IL-12 / STAT4 and IL-4 / STAT6 these two signaling pathways of Th1 cells and Th2 cells in the body of the gene expression, inhibiting the Th1 polarization and adjusting the imbalance of Thl/Th2 cells, so as to lower inflammatory response mediated by T cells and various kinds of pathological damage.展开更多
Economicdevelopment and population growth havedeeplydamaged the urbanwater environment of Guilin City, China. Main problems involved structuraldamage and functionaldeterioration of the urbanwaters. An integrated techn...Economicdevelopment and population growth havedeeplydamaged the urbanwater environment of Guilin City, China. Main problems involved structuraldamage and functionaldeterioration of the urbanwaters. An integrated technical schemewasdeveloped to rehabilitate the urbanwater environment and to enhance thewaters＇ functionsduring 1998-2008. Improvement ofwaters＇ functions includedwater system reconstruction,water pollution control,water safety assurance, and aquatic ecological restoration. Thewater systemwas reconstructed to connectdifferentwaters and cleanwater supplies to the lakes. Moreover,water pollutionwas controlled to improvewater quality by endogenous pollutant elimination and extraneous pollutant interception. In addition, ecological measures put in place serve to enhancewater system functions and better benefit both nature and humans. The project has brought about sound ecological, economic and social benefits in Guilin City,which can potentially be extended to similar cities.展开更多
DNA hydrogel is one of DNA-based nanomaterials with unique advantages such as precise self-assembly,programmability,addressability,high stability,excellent biocompatibility and biodegradability,and tunable versatility...DNA hydrogel is one of DNA-based nanomaterials with unique advantages such as precise self-assembly,programmability,addressability,high stability,excellent biocompatibility and biodegradability,and tunable versatility.These features have greatly promoted the development of DNA hydrogels in various applications,especially molecular diagnostics,biosensing,drug delivery,and cancer therapy.In this review,we briefly review the history of DNA hydrogels,the latest advances of DNA hydrogels in biomedical applications especially in biosensing,drug delivery and cancer therapy,and prospect the key challenges and future directions.展开更多
Using synthetic nanorobots to efficiently and safely execute therapeutic functions in human bodies is a long-standing dream.In the recent issue of Nature Biotechnology,a paper describes the latest development in such ...Using synthetic nanorobots to efficiently and safely execute therapeutic functions in human bodies is a long-standing dream.In the recent issue of Nature Biotechnology,a paper describes the latest development in such an attempt to implement an autonomous DNA robot in cancer therapy[1].Upon being injected into animals,the DNA nanorobots can find and destroy life-threatening tumors.展开更多
The G-quadruplexes undergo complex folding and conformation exchanges.G-quadruplex stability is substantially influenced by sequence,bufer and temperature.Mutational analysis together with nuclear magnetic resonance s...The G-quadruplexes undergo complex folding and conformation exchanges.G-quadruplex stability is substantially influenced by sequence,bufer and temperature.Mutational analysis together with nuclear magnetic resonance spectroscopy(NMR),X-ray crystallography and circular dichroism spectroscopy has been proved to be a powerful approach for G-quadruplex structural analysis.Herein,we used DNA sequence mutation and native polyacrylamide gel electrophoresis to investigate the topology and conformations of a G-quadruplex model molecule Pu18 found in the human c-MYC promoter.The guanines(G6,G9 or G18)which were not contributable to G-tetrad formation in c-MYC Pu18 sequence were mutated to thymine or adenine.We screened the buffer and temperature of gel electrophoresis for Pu18.Gel electrophoresis showed that two of the four conformers of c-MYC Pu18 in 100 mM K+buffer were resolved,which was in accordance with the conformations as determined by the 1 H NMR spectra in previous studies.This technique is expected as a general methodology for its easy operation and low cost to facilitate uncovering more yet unidentified G-quadruplex folds and functions,with the assistance of other analytical methods like NMR,X-ray crystallography and circular dichroism spectroscopy.展开更多
Correction to:Journal of Analysis and Testing(2021)5:188-194 https://doi.org/10.1007/s41664-021-00181-0 In the original publication of the article,the first author’s first name has been incorrectly published.The corr...Correction to:Journal of Analysis and Testing(2021)5:188-194 https://doi.org/10.1007/s41664-021-00181-0 In the original publication of the article,the first author’s first name has been incorrectly published.The correct author name is Xiao-Li Hu.The original article was corrected.展开更多
文摘Objective:To investigate the effect of Maxingganshi decoction on the expression of STAT4 and STAT6 in lung tissues of rats with chronic obstructive pulmonary disease (COPD).Methods:A total of 30 COPD Wistar rat models with phlegm-heat depression and lung depression were established, with half male and half female. The 30 model rats were fed in different cages, weighed and labeled. They were randomly divided into three groups: Maxingganshi decoction group, roxithromycin tablets control group, model control group. At the same time, 10 normal rats were selected as a blank control group. The white blood cell count and other cell count levels in the alveolar lavage fluid of the rats in the four groups, as well as the STAT4 and STAT6 protein levels in the lung tissues, were observed and compared.Results: After treatment, the white cell counts in Maxingganshi decoction group were significantly higher than that of the model control group (P<0.05);lymph, neutral particles and eosinophil levels were significantly lower than those of the model control group (P<0.05). Compared with roxithromycin tablets control group, white blood cell count and other classification level of cell count in Maxingganshi decoction group were not significantly different (P>0.05). After treatment, STAT4 protein levels in roxithromycin tablets control group and Maxingganshi decoction group were lower than that in the model control group (P<0.05), and STAT6 protein levels in roxithromycin tablets control group and Maxingganshi decoction group were higher than that of the model control group (P<0.05), suggesting the two means of intervention in this study could inhabit the STAT4 protein expression in lung tissue of COPD rats and promote effect on STAT6 protein. In addition, the level of STAT4 and STAT6 in the Maxingganshi decoction group was not significantly different from that in the roxithromycin tablets control group (P>0.05), suggesting that the effect of Maxingganshi decoction was similar to that of roxithromycin tablets.Conclusions: The action mechanism of Maxingganshi decoction group treating COPD may be through the STAT4 and STAT6 protein expression level to impose an effect, and thus interfere with IL-12 / STAT4 and IL-4 / STAT6 these two signaling pathways of Th1 cells and Th2 cells in the body of the gene expression, inhibiting the Th1 polarization and adjusting the imbalance of Thl/Th2 cells, so as to lower inflammatory response mediated by T cells and various kinds of pathological damage.
基金supported by the National Natural Science Foundation of China(No.51278055,51179008)the National Key Technology R&D Program(No.2012BAJ21B08)
文摘Economicdevelopment and population growth havedeeplydamaged the urbanwater environment of Guilin City, China. Main problems involved structuraldamage and functionaldeterioration of the urbanwaters. An integrated technical schemewasdeveloped to rehabilitate the urbanwater environment and to enhance thewaters＇ functionsduring 1998-2008. Improvement ofwaters＇ functions includedwater system reconstruction,water pollution control,water safety assurance, and aquatic ecological restoration. Thewater systemwas reconstructed to connectdifferentwaters and cleanwater supplies to the lakes. Moreover,water pollutionwas controlled to improvewater quality by endogenous pollutant elimination and extraneous pollutant interception. In addition, ecological measures put in place serve to enhancewater system functions and better benefit both nature and humans. The project has brought about sound ecological, economic and social benefits in Guilin City,which can potentially be extended to similar cities.
基金supported by the National Natural Science of China(31972622)the Fundamental Research Funds for the Central Universities(XDJK2020TJ001 and XDJK2020C049)
文摘DNA hydrogel is one of DNA-based nanomaterials with unique advantages such as precise self-assembly,programmability,addressability,high stability,excellent biocompatibility and biodegradability,and tunable versatility.These features have greatly promoted the development of DNA hydrogels in various applications,especially molecular diagnostics,biosensing,drug delivery,and cancer therapy.In this review,we briefly review the history of DNA hydrogels,the latest advances of DNA hydrogels in biomedical applications especially in biosensing,drug delivery and cancer therapy,and prospect the key challenges and future directions.
文摘Using synthetic nanorobots to efficiently and safely execute therapeutic functions in human bodies is a long-standing dream.In the recent issue of Nature Biotechnology,a paper describes the latest development in such an attempt to implement an autonomous DNA robot in cancer therapy[1].Upon being injected into animals,the DNA nanorobots can find and destroy life-threatening tumors.
基金the National Natural Science Foundation of China(21974111)Chongqing Research Program of Basic Research and Frontier Technology,China(cstc2018jcyjAX0482 and cstc2020jcyjmsxmX0947)Venture&Innovation Support Program for Chongqing Overseas Returnees(cx2018088)
文摘The G-quadruplexes undergo complex folding and conformation exchanges.G-quadruplex stability is substantially influenced by sequence,bufer and temperature.Mutational analysis together with nuclear magnetic resonance spectroscopy(NMR),X-ray crystallography and circular dichroism spectroscopy has been proved to be a powerful approach for G-quadruplex structural analysis.Herein,we used DNA sequence mutation and native polyacrylamide gel electrophoresis to investigate the topology and conformations of a G-quadruplex model molecule Pu18 found in the human c-MYC promoter.The guanines(G6,G9 or G18)which were not contributable to G-tetrad formation in c-MYC Pu18 sequence were mutated to thymine or adenine.We screened the buffer and temperature of gel electrophoresis for Pu18.Gel electrophoresis showed that two of the four conformers of c-MYC Pu18 in 100 mM K+buffer were resolved,which was in accordance with the conformations as determined by the 1 H NMR spectra in previous studies.This technique is expected as a general methodology for its easy operation and low cost to facilitate uncovering more yet unidentified G-quadruplex folds and functions,with the assistance of other analytical methods like NMR,X-ray crystallography and circular dichroism spectroscopy.
文摘Correction to:Journal of Analysis and Testing(2021)5:188-194 https://doi.org/10.1007/s41664-021-00181-0 In the original publication of the article,the first author’s first name has been incorrectly published.The correct author name is Xiao-Li Hu.The original article was corrected.
