Thyroid hormones have a specific effect on glucose-induced insulin secretion from the pancreas.We aimed to investigate the association between euthyroid hormones and islet betacell function in general population and n...Thyroid hormones have a specific effect on glucose-induced insulin secretion from the pancreas.We aimed to investigate the association between euthyroid hormones and islet betacell function in general population and non-treated type 2 diabetes mellitus(T2DM)patients.A total of 5089 euthyroid participants(including 4601 general population and 488 non-treated T2DM patients)were identified from a cross-sectional survey on the prevalence of metabolic diseases and risk factors in East China from February 2014 to June 2016.Anthropometric indices,biochemical parameters,and thyroid hormones were measured.Compared with general population,non-treated T2DM patients exhibited higher total thyroxine(TT4)and free thyroxine(FT4)levels but lower ratio of free triiodothyronine(T3):T4(P<0.01).HOMA-βhad prominently negative correlation with FT4 and positive relationship with free T3:T4 in both groups even after adjusting for age,body mass index(BMI)and smoking.When analyzed by quartiles of FT4 or free T3:T4,there were significantly decreased trend of HOMA-β going with the higher FT4 and lower free T3:T4 in both groups.Linear regression analysis showed that FT4 but not FT3 and free T3:T4 was negatively associated with HOMA-β no matter in general population or T2DM patients,which was independent of age,BMI,smoking,hypertension and lipid profiles.FT4 is independently and negatively associated with islet beta-cell function in euthyroid subjects.Thyroid hormone even in reference range could play an important role in the function of pancreatic islets.展开更多
The seabed instability induced by the transient liquefaction when exposed to wave-current may threaten the safety of offshore structures.In this study,the Reynolds-averaged Navier-Stokes(RANS)equations with the k-e tu...The seabed instability induced by the transient liquefaction when exposed to wave-current may threaten the safety of offshore structures.In this study,the Reynolds-averaged Navier-Stokes(RANS)equations with the k-e turbulence model were used to imitate the fluid dynamics,and Biot's poro-elastic theory was used to simulate the transient seabed response.An in-house solver(porous-fluid-seabed-structure interactions-field operation and manipulation)integrating the flow model and seabed model with the finite volume method was developed.The present model was confirmed with published experimental results and then used to analyze the dynamic process of the fluid-seabed-structure interactions as well as seafloor liquefaction around the jacket foundation under wave-current loading.The simulated results showed that the depth and range for the liquefaction area around the jacket foundation tended to increase at first and then declined as the wave propagated forward in the absence of current.In addition,the results demonstrated that the liquefaction depth under current and wave in the same orientation was greater than that without current.It is worth mentioning that the downstream piles were more prone to liquefaction than the upstream piles when the forward current existed.展开更多
In this study,we investigated the genetics,clinical features,and therapeutic approach of 14 patients with 5a-reductase deficiency in China.Genotyping analysis was performed by direct sequencing of PCR products of the ...In this study,we investigated the genetics,clinical features,and therapeutic approach of 14 patients with 5a-reductase deficiency in China.Genotyping analysis was performed by direct sequencing of PCR products of the steroid 5α-reductase type 2 gene(SRD5A2).The 5a-reductase activities of three novel mutations were investigated by mutagenesis and an in vitro transfection assay.Most patients presented with a microphallus,variable degrees of hypospadias,and cryptorchidism.Eight of 14 patients(57.1%)were initially reared as females and changed their social gender from female to male after puberty.Nine mutations were identified in the 14 patients.p.G203S,p.Q6X,and p.R227Q were the most prevalent mutations.Three mutations(p.K35N,p.H162P,and p.Y136X)have not been reported previously.The nonsense mutation p.Y136X abolished enzymatic activity,whereas p.K35N and p.H162P retained partial enzymatic activity.Topical administration of dihydrotestosterone during infancy or early childhood combined with hypospadia repair surgery had good therapeutic results.In conclusion,we expand the mutation profile of SRD5A2 in the Chinese population.A rational clinical approach to this disorder requires early and accurate diagnosis,especially genetic diagnosis.展开更多
Background:China has experienced rapid urbanization in the past 30 years.We aimed to report blood cadmium level(BCL)in the rapidly urbanized Yangtze Plain of China,and explore the association between BCL and 25-hydrox...Background:China has experienced rapid urbanization in the past 30 years.We aimed to report blood cadmium level(BCL)in the rapidly urbanized Yangtze Plain of China,and explore the association between BCL and 25-hydroxyvitamin D(25(OH)D).Methods:Our data source was the Survey on Prevalence in East China for Metabolic Diseases and Risk Factors(SPECT-China)cross-sectional study(ChiCTR-ECS-14005052,www.chictr.org).We enrolled 3234 subjects from 12 villages in the Yangtze Plain.BCLs were measured by atomic absorption spectrometry.25(OH)D was measured with a chemiluminescence assay.Results:A total of 2560(79.2%)subjects were diagnosed with vitamin D deficiency.The median(interquartile range)BCL was 1.80μg/L(0.60-3.42)for men and 1.40μg/L(0.52-3.10)for women.In women,mean 25(OH)D concentrations were inversely associated with BCL(0.401,95%confidence interval:-0.697 to-0.105 nmol/L lower with each doubling of the BCL)after adjustment for age,educational status,current smoking,body mass index,diabetes,and season.However,there was no significant difference in 25(OH)D across the BCL tertiles for men.Conclusions:BCL in Chinese residents in the Yangtze Plain were much higher than that in developed countries.An inverse association between BCL and 25(OH)D was found in general Chinese women after multivariable adjustment.Future prospective cohort and animal studies are warranted to resolve the direction and temporality of these relationships,and to elucidate the exact mechanisms involved.展开更多
基金grants from the National Natural Science Foundation of China(No.81270885,No.81570726 and No.81600609)Shanghai Jiao Tong University School of Medicine(2014)+4 种基金the Ministry of Science and Technology in China(No.2012CB524906)the Science and Technology Commission of Shanghai Municipality(No.14495810700 and No.16410723200)Three-year Action Plan for Public Health System Construction in Shanghai by Shanghai Municipal Commission of Health and Family Planning(2015-2017)Clinical Potential Subject Construction of Shanghai Jiao Tong University School of Medicine(2014),Shanghai Municipal Health Bureau of China(No.20124262)Seed Founding of Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine(No.JYZZ032).
文摘Thyroid hormones have a specific effect on glucose-induced insulin secretion from the pancreas.We aimed to investigate the association between euthyroid hormones and islet betacell function in general population and non-treated type 2 diabetes mellitus(T2DM)patients.A total of 5089 euthyroid participants(including 4601 general population and 488 non-treated T2DM patients)were identified from a cross-sectional survey on the prevalence of metabolic diseases and risk factors in East China from February 2014 to June 2016.Anthropometric indices,biochemical parameters,and thyroid hormones were measured.Compared with general population,non-treated T2DM patients exhibited higher total thyroxine(TT4)and free thyroxine(FT4)levels but lower ratio of free triiodothyronine(T3):T4(P<0.01).HOMA-βhad prominently negative correlation with FT4 and positive relationship with free T3:T4 in both groups even after adjusting for age,body mass index(BMI)and smoking.When analyzed by quartiles of FT4 or free T3:T4,there were significantly decreased trend of HOMA-β going with the higher FT4 and lower free T3:T4 in both groups.Linear regression analysis showed that FT4 but not FT3 and free T3:T4 was negatively associated with HOMA-β no matter in general population or T2DM patients,which was independent of age,BMI,smoking,hypertension and lipid profiles.FT4 is independently and negatively associated with islet beta-cell function in euthyroid subjects.Thyroid hormone even in reference range could play an important role in the function of pancreatic islets.
基金supported by the Qingdao Postdoctoral Researcher Applied Research Project.
文摘The seabed instability induced by the transient liquefaction when exposed to wave-current may threaten the safety of offshore structures.In this study,the Reynolds-averaged Navier-Stokes(RANS)equations with the k-e turbulence model were used to imitate the fluid dynamics,and Biot's poro-elastic theory was used to simulate the transient seabed response.An in-house solver(porous-fluid-seabed-structure interactions-field operation and manipulation)integrating the flow model and seabed model with the finite volume method was developed.The present model was confirmed with published experimental results and then used to analyze the dynamic process of the fluid-seabed-structure interactions as well as seafloor liquefaction around the jacket foundation under wave-current loading.The simulated results showed that the depth and range for the liquefaction area around the jacket foundation tended to increase at first and then declined as the wave propagated forward in the absence of current.In addition,the results demonstrated that the liquefaction depth under current and wave in the same orientation was greater than that without current.It is worth mentioning that the downstream piles were more prone to liquefaction than the upstream piles when the forward current existed.
基金This study is supported by grants from the National Natural Science Foundation(No.81570753,81430019 and 81873652)the Shanghai Jiao Tong University Medical and Engineering Intersection Foundation(No.YG2015QN03).
文摘In this study,we investigated the genetics,clinical features,and therapeutic approach of 14 patients with 5a-reductase deficiency in China.Genotyping analysis was performed by direct sequencing of PCR products of the steroid 5α-reductase type 2 gene(SRD5A2).The 5a-reductase activities of three novel mutations were investigated by mutagenesis and an in vitro transfection assay.Most patients presented with a microphallus,variable degrees of hypospadias,and cryptorchidism.Eight of 14 patients(57.1%)were initially reared as females and changed their social gender from female to male after puberty.Nine mutations were identified in the 14 patients.p.G203S,p.Q6X,and p.R227Q were the most prevalent mutations.Three mutations(p.K35N,p.H162P,and p.Y136X)have not been reported previously.The nonsense mutation p.Y136X abolished enzymatic activity,whereas p.K35N and p.H162P retained partial enzymatic activity.Topical administration of dihydrotestosterone during infancy or early childhood combined with hypospadia repair surgery had good therapeutic results.In conclusion,we expand the mutation profile of SRD5A2 in the Chinese population.A rational clinical approach to this disorder requires early and accurate diagnosis,especially genetic diagnosis.
基金supported by grants from the National Natural Science Foundation of China(No.81600609)Science and Technology Commission of Shanghai Municipality(No.18410722300)+2 种基金Municipal Human Resources Development Program for Outstanding Young Talents in Medical and Health Sciences in Shanghai(No.2017YQ053)Fundamental Research Program Funding of Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine(No.JYZZ099)Shanghai Sailing Program(No.20YF1423500)。
文摘Background:China has experienced rapid urbanization in the past 30 years.We aimed to report blood cadmium level(BCL)in the rapidly urbanized Yangtze Plain of China,and explore the association between BCL and 25-hydroxyvitamin D(25(OH)D).Methods:Our data source was the Survey on Prevalence in East China for Metabolic Diseases and Risk Factors(SPECT-China)cross-sectional study(ChiCTR-ECS-14005052,www.chictr.org).We enrolled 3234 subjects from 12 villages in the Yangtze Plain.BCLs were measured by atomic absorption spectrometry.25(OH)D was measured with a chemiluminescence assay.Results:A total of 2560(79.2%)subjects were diagnosed with vitamin D deficiency.The median(interquartile range)BCL was 1.80μg/L(0.60-3.42)for men and 1.40μg/L(0.52-3.10)for women.In women,mean 25(OH)D concentrations were inversely associated with BCL(0.401,95%confidence interval:-0.697 to-0.105 nmol/L lower with each doubling of the BCL)after adjustment for age,educational status,current smoking,body mass index,diabetes,and season.However,there was no significant difference in 25(OH)D across the BCL tertiles for men.Conclusions:BCL in Chinese residents in the Yangtze Plain were much higher than that in developed countries.An inverse association between BCL and 25(OH)D was found in general Chinese women after multivariable adjustment.Future prospective cohort and animal studies are warranted to resolve the direction and temporality of these relationships,and to elucidate the exact mechanisms involved.
