Transplantation of activated transgenic Schwann cells or a fetal spinal cord cell suspension has been widely used to treat spinal cord injury. However, little is known regarding the effects of co-transplantation. In t...Transplantation of activated transgenic Schwann cells or a fetal spinal cord cell suspension has been widely used to treat spinal cord injury. However, little is known regarding the effects of co-transplantation. In the present study, autologous Schwann cells in combination with a fetal spinal cord cell suspension were transplanted into adult Wistar rats with spinal cord injury, and newly generated axonal connections were observed ultrastructurally. Transmission electron microscopic observations showed that the neuroblast first presented cytoplasmic processes, followed by pre- and postsynaptic membranes with low electron density forming a dense projection. The number and types of synaptic vesicles were increased. Synaptic connections developed from single cell body-dendritic synapses into multiple cell body-dendritic and dendrite-dendritic synapses. In addition, the cell organs of the transplanted neuroblast, oligodendroblast and astroblast matured gradually. The blood-brain barrier appeared subsequently. Moreover, neurofilament, histamine, calcitonin-gene-related peptides, and glial fibrillary acidic protein positive fibers were observed in the transplant region. These findings demonstrate that fetal spinal cord cells in the presence of autologous activated Schwann cells can develop into mature synapses in the cavity of injured spinal cords, suggesting the possibility of information exchange through the reconstructed synapse between fetal spinal cord cells and the host.展开更多
This work develops a distributed environmental monitoring system for the combination of hydroponics and aquaculture based on the internet of things technology,which mainly includes the information perception layer,the...This work develops a distributed environmental monitoring system for the combination of hydroponics and aquaculture based on the internet of things technology,which mainly includes the information perception layer,the information transmission layer and the sys-tem architecture.The system has employed multiple sensors terminal to real-time acqui-sition,including air and water temperatures,dissolved oxygen etc.LoRa protocol is suitable for sending small data and the 4G was employed to collect data and send to the cloud plat-form.Java is used to develop background applications,to access cloud platforms and local data processing.Based on the collection and processing of environmental data and cloud service platform,the mobile application program client and remote login desktop have been developed.It has been implemented and tested in Tongzhou,Beijing for 3 months in 2020.The results showed the proposed monitoring system stability for overall operation and accuracy data transmission,which can support the actual hydroponics and aquacul-ture production management.After analysis of monitoring data collected from the devel-oped monitoring system,indoor air and water temperature have the obvious correlation with atmospheric pressure(0.7 and 0.9)and outdoor temperature(1.0 and 0.9),respectively.展开更多
基金the Tianjin Science and Technology Commission Key Project,No.07JCZDJC08000the Natural Science Foundation of China, No.30772193,30571876National High-Tech R&D Program of China (863 Program),No.2007AA04Z235
文摘Transplantation of activated transgenic Schwann cells or a fetal spinal cord cell suspension has been widely used to treat spinal cord injury. However, little is known regarding the effects of co-transplantation. In the present study, autologous Schwann cells in combination with a fetal spinal cord cell suspension were transplanted into adult Wistar rats with spinal cord injury, and newly generated axonal connections were observed ultrastructurally. Transmission electron microscopic observations showed that the neuroblast first presented cytoplasmic processes, followed by pre- and postsynaptic membranes with low electron density forming a dense projection. The number and types of synaptic vesicles were increased. Synaptic connections developed from single cell body-dendritic synapses into multiple cell body-dendritic and dendrite-dendritic synapses. In addition, the cell organs of the transplanted neuroblast, oligodendroblast and astroblast matured gradually. The blood-brain barrier appeared subsequently. Moreover, neurofilament, histamine, calcitonin-gene-related peptides, and glial fibrillary acidic protein positive fibers were observed in the transplant region. These findings demonstrate that fetal spinal cord cells in the presence of autologous activated Schwann cells can develop into mature synapses in the cavity of injured spinal cords, suggesting the possibility of information exchange through the reconstructed synapse between fetal spinal cord cells and the host.
基金This research was financially supported by Research on Industrialization of Real-time Measurement and Control Technology for Efficient Fish-vegetable Symbiosis System(Project No.:KJ2019CX099).
文摘This work develops a distributed environmental monitoring system for the combination of hydroponics and aquaculture based on the internet of things technology,which mainly includes the information perception layer,the information transmission layer and the sys-tem architecture.The system has employed multiple sensors terminal to real-time acqui-sition,including air and water temperatures,dissolved oxygen etc.LoRa protocol is suitable for sending small data and the 4G was employed to collect data and send to the cloud plat-form.Java is used to develop background applications,to access cloud platforms and local data processing.Based on the collection and processing of environmental data and cloud service platform,the mobile application program client and remote login desktop have been developed.It has been implemented and tested in Tongzhou,Beijing for 3 months in 2020.The results showed the proposed monitoring system stability for overall operation and accuracy data transmission,which can support the actual hydroponics and aquacul-ture production management.After analysis of monitoring data collected from the devel-oped monitoring system,indoor air and water temperature have the obvious correlation with atmospheric pressure(0.7 and 0.9)and outdoor temperature(1.0 and 0.9),respectively.
