AIM:To explore the effect of silent information regulator factor 2-related enzyme 1(SIRT1)on modulating apoptosis of human lens epithelial cells(HLECs)and alleviating lens opacification of rats through suppressing end...AIM:To explore the effect of silent information regulator factor 2-related enzyme 1(SIRT1)on modulating apoptosis of human lens epithelial cells(HLECs)and alleviating lens opacification of rats through suppressing endoplasmic reticulum(ER)stress.METHODS:HLECs(SRA01/04)were treated with varying concentrations of tunicamycin(TM)for 24h,and the expression of SIRT1 and C/EBP homologous protein(CHOP)was assessed using real-time quantitative polymerase chain reaction(RT-PCR),Western blotting,and immunofluorescence.Cell morphology and proliferation was evaluated using an inverted microscope and cell counting kit-8(CCK-8)assay,respectively.In the SRA01/04 cell apoptosis model,which underwent siRNA transfection for SIRT1 knockdown and SRT1720 treatment for its activation,the expression levels of SIRT1,CHOP,glucose regulated protein 78(GRP78),and activating transcription factor 4(ATF4)were examined.The potential reversal of SIRT1 knockdown effects by 4-phenyl butyric acid(4-PBA;an ER stress inhibitor)was investigated.In vivo,age-related cataract(ARC)rat models were induced by sodium selenite injection,and the protective role of SIRT1,activated by SRT1720 intraperitoneal injections,was evaluated through morphology observation,hematoxylin and eosin(H&E)staining,Western blotting,and RT-PCR.RESULTS:SIRT1 expression was downregulated in TMinduced SRA01/04 cells.Besides,in SRA01/04 cells,both cell apoptosis and CHOP expression increased with the rising doses of TM.ER stress was stimulated by TM,as evidenced by the increased GRP78 and ATF4 in the SRA01/04 cell apoptosis model.Inhibition of SIRT1 by siRNA knockdown increased ER stress activation,whereas SRT1720 treatment had opposite results.4-PBA partly reverse the adverse effect of SIRT1 knockdown on apoptosis.In vivo,SRT1720 attenuated the lens opacification and weakened the ER stress activation in ARC rat models.CONCLUSION:SIRT1 plays a protective role against TM-induced apoptosis in HLECs and slows the progression of cataract in rats by inhibiting ER stress.These findings suggest a novel strategy for cataract treatment focused on targeting ER stress,highlighting the therapeutic potential of SIRT1 modulation in ARC development.展开更多
. Chinese and foreign film and cultural exchanges, the translation is necessary to ensure the realization of information across language barriers to effective communication. Particularity TV drama text language determ.... Chinese and foreign film and cultural exchanges, the translation is necessary to ensure the realization of information across language barriers to effective communication. Particularity TV drama text language determines the TV drama translation from other forms of translation particularity. According to the analysis of the speci？ c case, we discussed the basics of film and television drama translation. In recent years, foreign film and television works more and more popular in China, Subtitling received more attention. Filmography is an important medium for intercultural communication that subtitling has a very important role in cross-cultural communication. By analyzing the subtitle translation of particularity, we propose the appropriate translation approach. News translation is an interlingual intercultural communication activities and news translation there are a lot of cultural barriers that improper handling will seriously affect the effect of news media, and even misleading. News translator should understand the source culture, and appropriate use of domesticating translation strategy interpretive translation means to overcome cultural barriers in order to achieve intercultural communication.展开更多
With the deepening of China’s power market, bilateral transactions will continue to grow in large scale. The release of bilateral transactions locked more regulatory resources of the power grid, will directly affect ...With the deepening of China’s power market, bilateral transactions will continue to grow in large scale. The release of bilateral transactions locked more regulatory resources of the power grid, will directly affect the operation mode of the unit and the implementation of planned electricity. In the paper, considering the large-scale bilateral trade effect on the peak regulation of power grid, energy saving and emission reduction, power system security and other factors, and then putting forward the method of long term generation planning and annual planning model to adapt to the safe operation of power grid in China. In the model, the target is minimizing the monthly load rate deviation and the annual electric quantity deviation rate, the latter includes the capacity factor. In addition, the constraints include the monthly quantity of electricity, adjustable utilization rate deviation, load rate, reserve and key sections, etc. Through an example to verify the correctness of the model, the planning and power transaction results can satisfy the peak regulation of load, energy saving and emission reduction and safety operation of the power grid requirements.展开更多
AIM:To present the clinical manifestations of 5 autosomal dominant cone-rod dystrophy(ad CORD)patients from two Chinese families with cone-rod homeobox(CRX)mutation(p.R41W),and to explore the clinical heterogeneity of...AIM:To present the clinical manifestations of 5 autosomal dominant cone-rod dystrophy(ad CORD)patients from two Chinese families with cone-rod homeobox(CRX)mutation(p.R41W),and to explore the clinical heterogeneity of ad CORD with CRX mutation(p.R41W).METHODS:Interrogation and ophthalmological examinations were undertaken in all patients and unaffected members.Analysis of clinical features was performed by visual acuity,slit lamp examination,visual field examination,fundoscopy,autofluorescence and spectral domain optical coherence tomography.Targeted next-generation sequencing was applied as a useful tool to identify the causative mutation of CORD genes.RESULTS:A CRX missense mutation c.121C>T was identified in all patients,resulting in an amino acid change from arginine acid to tryptophan(p.R41W).The patients presented with early onset,progressive and different severities with CORD.CONCLUSION:This is the first report of the clinical phenotype of CRX mutation(p.R41W)in Chinese families,and the mutation can lead to a wide range of various retinal phenotypes.展开更多
Carbon nanotubes(CNTs)have attracted great interest in numerous applications due to their excellent electronic and structural properties.However,harsh synthesis conditions and high energy consumption limit the further...Carbon nanotubes(CNTs)have attracted great interest in numerous applications due to their excellent electronic and structural properties.However,harsh synthesis conditions and high energy consumption limit the further application of CNTs.Herein,we developed a facile and innovative strategy to generate CNTs by physically pressurizing metal-organic frameworks(MOFs)and then pyrolyzing.The synthesized bimetallic nitrogen/sulfur double-doped carbon nanotubes are denoted as Fe_(x)Co_(1-x)P(x=0.5,0.6,0.7,0.8,0.9).The introduction of pressure not only revolutionized the morphology of the electrocatalysts,but also enhanced the activity of oxygen evolution reaction(OER)and hydrogen evolution reaction(HER)in alkaline solution.Fe_(0.6)Co_(0.4)-P exhibits superior OER and HER performances in 1.0 mol·L^(-1)KOH solution with overpotentials of 364 and 346 mV at 10 mA·cm^(-2),which are 28 and 5 mV lower than unpressurized Fe0.6Co0.4.展开更多
Electrocatalysts with high activities are crucial for highenergy-density Zn-air batteries.However,the sluggish kinetics of oxygen evolution reaction(OER)and oxygen reduction reaction(ORR)on the electrocatalysts hinder...Electrocatalysts with high activities are crucial for highenergy-density Zn-air batteries.However,the sluggish kinetics of oxygen evolution reaction(OER)and oxygen reduction reaction(ORR)on the electrocatalysts hindered the development of Zn-air batteries.Herein,a new class of MOF-derived nitrogen-doped carbon nanotubes encapsulated with bimetallic oxide(FeNiO@NCNT)through facile pyrolysis strategy is reported.The FeNiO@NCNT exhibits high catalytic activities for both OER and ORR.展开更多
In this paper, we prepared a dual functional system based on dextrin-coated silver nanoparticles which were further attached with iron oxide nanoparticles and cell penetrating peptide(Tat), producing Tat-modified Ag-F...In this paper, we prepared a dual functional system based on dextrin-coated silver nanoparticles which were further attached with iron oxide nanoparticles and cell penetrating peptide(Tat), producing Tat-modified Ag-Fe_3O_4 nanocomposites(Tat-FeAgNPs). To load drugs, an –SH containing linker, 3-mercaptopropanohydrazide, was designed and synthesized. It enabled the silver carriers to load and release doxorubicin(Dox) in a pH-sensitive pattern. The delivery efficiency of this system was assessed in vitro using MCF-7 cells, and in vivo using null BalB/c mice bearing MCF-7 xenograft tumors. Our results demonstrated that both Tat and externally applied magnetic field could promote cellular uptake and consequently the cytotoxicity of doxorubicin-loaded nanoparticles,with the IC_(50) of Tat-FeAgNP-Dox to be 0.63 mmol/L. The in vivo delivery efficiency of Tat-FeAgNP carrying Cy5 to the mouse tumor was analyzed using the in vivo optical imaging tests, in which TatFeAgNP-Cy5 yielded the most efficient accumulation in the tumor(6.772.4% ID of Tat-FeAgNPs).Anti-tumor assessment also demonstrated that Tat-FeAgNP-Dox displayed the most significant tumor-inhibiting effects and reduced the specific growth rate of tumor by 29.6%(P ? 0.009), which could be attributed to its superior performance in tumor drug delivery in comparison with the control nanovehicles.展开更多
Accurate recognition of modern and traditional porcelain styles is a challenging issue in Cantonese porcelain management due to the large variety and complex elements and patterns. We propose a hybrid system with porc...Accurate recognition of modern and traditional porcelain styles is a challenging issue in Cantonese porcelain management due to the large variety and complex elements and patterns. We propose a hybrid system with porcelain style identification and image recreation modules. In the identification module, prediction of an unknown porcelain sample is obtained by logistic regression of ensembled neural networks of top-ranked design signatures, which are obtained by discriminative analysis and transformed features in principal components. The synthesis module is developed based on a conditional generative adversarial network, which enables users to provide a designed mask with porcelain elements to generate synthesized images of Cantonese porcelain. Experimental results of 603 Cantonese porcelain images demonstrate that the proposed model outperforms other methods relative to precision, recall, area under curve of receiver operating characteristic, and confusion matrix. Case studies on image creation indicate that the proposed system has the potential to engage the community in understanding Cantonese porcelain and promote this intangible cultural heritage.展开更多
Protein phosphorylation, one of the most important protein post-translational modifications, is involved in various biological processes, and the identification of phosphorylation peptides （phosphopeptides） and thei...Protein phosphorylation, one of the most important protein post-translational modifications, is involved in various biological processes, and the identification of phosphorylation peptides （phosphopeptides） and their corresponding phosphorylation sites （phosphosites） will facilitate the understanding of the molecular mechanism and function of phosphorylation. Mass spectrometry （MS） provides a high- throughput technology that enables the identification of large numbers of phosphosites. PhoPepMass is designed to assist human phosphopeptide identification from MS data based on a specific database of phophopeptide masses and a multivariate hypergeometric matching algorithm. It contains 244,915 phosphosites from several public sources. Moreover, the accurate masses of peptides and fragments with phosphosites were calculated. It is the first database that provides a systematic resource for the query of phosphosites on peptides and their corresponding masses. This allows researchers to search certain proteins of which phosphosites have been reported, to browse detailed phosphopeptide and fragment information, to match masses from MS analyses with defined threshold to the corresponding phos- phopeptide, and to compare proprietary phosphopeptide discovery results with results from previous studies. Additionally, a database search software is created and a ＂two-stage search strategy＂ is suggested to identify phosphopeptides from tandem mass spectra of proteomics data. We expect PhoPepMass to be a useful tool and a source of reference for proteomics researchers.展开更多
基金Supported by National Natural Science Foundation for Young Scientists of China(No.82101097)National Natural Science Foundation of China(No.82070937).
文摘AIM:To explore the effect of silent information regulator factor 2-related enzyme 1(SIRT1)on modulating apoptosis of human lens epithelial cells(HLECs)and alleviating lens opacification of rats through suppressing endoplasmic reticulum(ER)stress.METHODS:HLECs(SRA01/04)were treated with varying concentrations of tunicamycin(TM)for 24h,and the expression of SIRT1 and C/EBP homologous protein(CHOP)was assessed using real-time quantitative polymerase chain reaction(RT-PCR),Western blotting,and immunofluorescence.Cell morphology and proliferation was evaluated using an inverted microscope and cell counting kit-8(CCK-8)assay,respectively.In the SRA01/04 cell apoptosis model,which underwent siRNA transfection for SIRT1 knockdown and SRT1720 treatment for its activation,the expression levels of SIRT1,CHOP,glucose regulated protein 78(GRP78),and activating transcription factor 4(ATF4)were examined.The potential reversal of SIRT1 knockdown effects by 4-phenyl butyric acid(4-PBA;an ER stress inhibitor)was investigated.In vivo,age-related cataract(ARC)rat models were induced by sodium selenite injection,and the protective role of SIRT1,activated by SRT1720 intraperitoneal injections,was evaluated through morphology observation,hematoxylin and eosin(H&E)staining,Western blotting,and RT-PCR.RESULTS:SIRT1 expression was downregulated in TMinduced SRA01/04 cells.Besides,in SRA01/04 cells,both cell apoptosis and CHOP expression increased with the rising doses of TM.ER stress was stimulated by TM,as evidenced by the increased GRP78 and ATF4 in the SRA01/04 cell apoptosis model.Inhibition of SIRT1 by siRNA knockdown increased ER stress activation,whereas SRT1720 treatment had opposite results.4-PBA partly reverse the adverse effect of SIRT1 knockdown on apoptosis.In vivo,SRT1720 attenuated the lens opacification and weakened the ER stress activation in ARC rat models.CONCLUSION:SIRT1 plays a protective role against TM-induced apoptosis in HLECs and slows the progression of cataract in rats by inhibiting ER stress.These findings suggest a novel strategy for cataract treatment focused on targeting ER stress,highlighting the therapeutic potential of SIRT1 modulation in ARC development.
文摘. Chinese and foreign film and cultural exchanges, the translation is necessary to ensure the realization of information across language barriers to effective communication. Particularity TV drama text language determines the TV drama translation from other forms of translation particularity. According to the analysis of the speci？ c case, we discussed the basics of film and television drama translation. In recent years, foreign film and television works more and more popular in China, Subtitling received more attention. Filmography is an important medium for intercultural communication that subtitling has a very important role in cross-cultural communication. By analyzing the subtitle translation of particularity, we propose the appropriate translation approach. News translation is an interlingual intercultural communication activities and news translation there are a lot of cultural barriers that improper handling will seriously affect the effect of news media, and even misleading. News translator should understand the source culture, and appropriate use of domesticating translation strategy interpretive translation means to overcome cultural barriers in order to achieve intercultural communication.
文摘With the deepening of China’s power market, bilateral transactions will continue to grow in large scale. The release of bilateral transactions locked more regulatory resources of the power grid, will directly affect the operation mode of the unit and the implementation of planned electricity. In the paper, considering the large-scale bilateral trade effect on the peak regulation of power grid, energy saving and emission reduction, power system security and other factors, and then putting forward the method of long term generation planning and annual planning model to adapt to the safe operation of power grid in China. In the model, the target is minimizing the monthly load rate deviation and the annual electric quantity deviation rate, the latter includes the capacity factor. In addition, the constraints include the monthly quantity of electricity, adjustable utilization rate deviation, load rate, reserve and key sections, etc. Through an example to verify the correctness of the model, the planning and power transaction results can satisfy the peak regulation of load, energy saving and emission reduction and safety operation of the power grid requirements.
文摘AIM:To present the clinical manifestations of 5 autosomal dominant cone-rod dystrophy(ad CORD)patients from two Chinese families with cone-rod homeobox(CRX)mutation(p.R41W),and to explore the clinical heterogeneity of ad CORD with CRX mutation(p.R41W).METHODS:Interrogation and ophthalmological examinations were undertaken in all patients and unaffected members.Analysis of clinical features was performed by visual acuity,slit lamp examination,visual field examination,fundoscopy,autofluorescence and spectral domain optical coherence tomography.Targeted next-generation sequencing was applied as a useful tool to identify the causative mutation of CORD genes.RESULTS:A CRX missense mutation c.121C>T was identified in all patients,resulting in an amino acid change from arginine acid to tryptophan(p.R41W).The patients presented with early onset,progressive and different severities with CORD.CONCLUSION:This is the first report of the clinical phenotype of CRX mutation(p.R41W)in Chinese families,and the mutation can lead to a wide range of various retinal phenotypes.
基金financially supported by the National Natural Science Foundation of China(No.52171277)Zhejiang Provincial Natural Science Foundation of China(No.LY20E020001)。
文摘Carbon nanotubes(CNTs)have attracted great interest in numerous applications due to their excellent electronic and structural properties.However,harsh synthesis conditions and high energy consumption limit the further application of CNTs.Herein,we developed a facile and innovative strategy to generate CNTs by physically pressurizing metal-organic frameworks(MOFs)and then pyrolyzing.The synthesized bimetallic nitrogen/sulfur double-doped carbon nanotubes are denoted as Fe_(x)Co_(1-x)P(x=0.5,0.6,0.7,0.8,0.9).The introduction of pressure not only revolutionized the morphology of the electrocatalysts,but also enhanced the activity of oxygen evolution reaction(OER)and hydrogen evolution reaction(HER)in alkaline solution.Fe_(0.6)Co_(0.4)-P exhibits superior OER and HER performances in 1.0 mol·L^(-1)KOH solution with overpotentials of 364 and 346 mV at 10 mA·cm^(-2),which are 28 and 5 mV lower than unpressurized Fe0.6Co0.4.
基金financially supported by Zhejiang Provincial Natural Science Foundation of China(No.LY20E020001)the Fundamental Research Funds of Zhejiang Sci-Tech University(No.22212290-Y)。
文摘Electrocatalysts with high activities are crucial for highenergy-density Zn-air batteries.However,the sluggish kinetics of oxygen evolution reaction(OER)and oxygen reduction reaction(ORR)on the electrocatalysts hindered the development of Zn-air batteries.Herein,a new class of MOF-derived nitrogen-doped carbon nanotubes encapsulated with bimetallic oxide(FeNiO@NCNT)through facile pyrolysis strategy is reported.The FeNiO@NCNT exhibits high catalytic activities for both OER and ORR.
基金financial supports from National Key Research and Development Plan of China (2016YFE0119200)the Young Elite Scientists Sponsorship Program by Tianjin (No. TJSQNTJ-2017-14)National Natural Science Foundation of China (NSFC 81361140344, 21376164, 81402885, and 81373357)
文摘In this paper, we prepared a dual functional system based on dextrin-coated silver nanoparticles which were further attached with iron oxide nanoparticles and cell penetrating peptide(Tat), producing Tat-modified Ag-Fe_3O_4 nanocomposites(Tat-FeAgNPs). To load drugs, an –SH containing linker, 3-mercaptopropanohydrazide, was designed and synthesized. It enabled the silver carriers to load and release doxorubicin(Dox) in a pH-sensitive pattern. The delivery efficiency of this system was assessed in vitro using MCF-7 cells, and in vivo using null BalB/c mice bearing MCF-7 xenograft tumors. Our results demonstrated that both Tat and externally applied magnetic field could promote cellular uptake and consequently the cytotoxicity of doxorubicin-loaded nanoparticles,with the IC_(50) of Tat-FeAgNP-Dox to be 0.63 mmol/L. The in vivo delivery efficiency of Tat-FeAgNP carrying Cy5 to the mouse tumor was analyzed using the in vivo optical imaging tests, in which TatFeAgNP-Cy5 yielded the most efficient accumulation in the tumor(6.772.4% ID of Tat-FeAgNPs).Anti-tumor assessment also demonstrated that Tat-FeAgNP-Dox displayed the most significant tumor-inhibiting effects and reduced the specific growth rate of tumor by 29.6%(P ? 0.009), which could be attributed to its superior performance in tumor drug delivery in comparison with the control nanovehicles.
基金Project supported by the Guangzhou Science and Technology Project,China(No.2018GZMZYB17)
文摘Accurate recognition of modern and traditional porcelain styles is a challenging issue in Cantonese porcelain management due to the large variety and complex elements and patterns. We propose a hybrid system with porcelain style identification and image recreation modules. In the identification module, prediction of an unknown porcelain sample is obtained by logistic regression of ensembled neural networks of top-ranked design signatures, which are obtained by discriminative analysis and transformed features in principal components. The synthesis module is developed based on a conditional generative adversarial network, which enables users to provide a designed mask with porcelain elements to generate synthesized images of Cantonese porcelain. Experimental results of 603 Cantonese porcelain images demonstrate that the proposed model outperforms other methods relative to precision, recall, area under curve of receiver operating characteristic, and confusion matrix. Case studies on image creation indicate that the proposed system has the potential to engage the community in understanding Cantonese porcelain and promote this intangible cultural heritage.
基金supported by National Key Research and Development Program of China (2016YFC0904101)Chinese Human Proteome Projects (CNHPP: 2014DFB30020, 2014DFB30030)+1 种基金Shanghai Municipal Science and Technology Commission of China (No. 17ZR1420300)National Hi-Tech Program (2015AA020101)
文摘Protein phosphorylation, one of the most important protein post-translational modifications, is involved in various biological processes, and the identification of phosphorylation peptides （phosphopeptides） and their corresponding phosphorylation sites （phosphosites） will facilitate the understanding of the molecular mechanism and function of phosphorylation. Mass spectrometry （MS） provides a high- throughput technology that enables the identification of large numbers of phosphosites. PhoPepMass is designed to assist human phosphopeptide identification from MS data based on a specific database of phophopeptide masses and a multivariate hypergeometric matching algorithm. It contains 244,915 phosphosites from several public sources. Moreover, the accurate masses of peptides and fragments with phosphosites were calculated. It is the first database that provides a systematic resource for the query of phosphosites on peptides and their corresponding masses. This allows researchers to search certain proteins of which phosphosites have been reported, to browse detailed phosphopeptide and fragment information, to match masses from MS analyses with defined threshold to the corresponding phos- phopeptide, and to compare proprietary phosphopeptide discovery results with results from previous studies. Additionally, a database search software is created and a ＂two-stage search strategy＂ is suggested to identify phosphopeptides from tandem mass spectra of proteomics data. We expect PhoPepMass to be a useful tool and a source of reference for proteomics researchers.
