Rickettsia felis is an exclusively cytozoic Gram-negative prokaryote with cat fleas as the major vectors.[1]As early as 1918,Rickettsia felis was detected in cat fleas in Europe and named Rickettsia ctenocephali.[2]Sy...Rickettsia felis is an exclusively cytozoic Gram-negative prokaryote with cat fleas as the major vectors.[1]As early as 1918,Rickettsia felis was detected in cat fleas in Europe and named Rickettsia ctenocephali.[2]Symptoms of fever,malaise,headache,maculopapular rash and eschar are observed in patients with Rickettsia felis infection.展开更多
BACKGROUND Total knee arthroplasty(TKA)is a mature procedure recommended for correcting knee osteoarthritis deformity,relieving pain,and restoring normal biomechanics.Although TKA is a successful and cost-effective pr...BACKGROUND Total knee arthroplasty(TKA)is a mature procedure recommended for correcting knee osteoarthritis deformity,relieving pain,and restoring normal biomechanics.Although TKA is a successful and cost-effective procedure,patient dissatisfaction is as high as 50%.Knee pain after TKA is a significant cause of patient dissatisfaction;the most common location for residual pain is the anterior region.Between 4%and 40%of patients have anterior knee pain(AKP).AIM To investigate the effect of various TKA procedures on postoperative AKP.METHODS We searched PubMed,EMBASE,and Cochrane from January 2000 to September 2022.Randomized controlled trials with one intervention in the experimental group and no corresponding intervention(or other interventions)in the control group were collected.Two researchers independently read the title and abstract of the studies,preliminarily screened the articles,and read the full text in detail according to the selection criteria.Conflicts were resolved by consultation with a third researcher.And relevant data from the included studies were extracted and analyzed using Review Manager 5.4 software.RESULTS There were 25 randomized controlled trials;13 were comparative studies with or without patellar resurfacing.The meta-analysis showed no significant difference between the experimental and control groups(P=0.61).Six studies were comparative studies of circumpatellar denervation vs non-denervation,divided into three subgroups for meta-analysis.The two-subgroup meta-analysis showed no significant difference between the experimental and the control groups(P=0.31,P=0.50).One subgroup meta-analysis showed a significant difference between the experimental and control groups(P=0.001).Two studies compared fixed-bearing TKA and mobile-bearing TKA;the results meta-analysis showed no significant difference between the experimental and control groups(P=0.630).Two studies compared lateral retinacular release vs non-release;the meta-analysis showed a significant difference between the experimental and control groups(P=0.002);two other studies compared other factors.CONCLUSION Patellar resurfacing,mobile-bearing TKA,and fixed-bearing TKA do not reduce the incidence of AKP.Lateral retinacular release can reduce AKP;however,whether circumpatellar denervation can reduce AKP is controversial.展开更多
Acute pancreatitis(AP)often leads to a high incidence of cardiac injury,posing significant challenges in the treatment of severe AP and contributing to increased mortality rates.Mesenchymal stem cells(MSCs)release bio...Acute pancreatitis(AP)often leads to a high incidence of cardiac injury,posing significant challenges in the treatment of severe AP and contributing to increased mortality rates.Mesenchymal stem cells(MSCs)release bioactive molecules that participate in various inflammatory diseases.Similarly,extracellular vesicles(EVs)secreted by MSCs have garnered extensive attention due to their comparable anti-inflammatory effects to MSCs and their potential to avoid risks associated with cell transplantation.Recently,the therapeutic potential of MSCs-EVs in various inflammatory diseases,including sepsis and AP,has gained increasing recognition.Although preclinical research on the utilization of MSCs-EVs in AP-induced cardiac injury is limited,several studies have demonstrated the positive effects of MSCs-EVs in regulating inflammation and immunity in sepsis-induced cardiac injury and cardiovascular diseases.Furthermore,clinical studies have been conducted on the therapeutic application of MSCs-EVs for some other diseases,wherein the contents of these EVs could be deliberately modified through prior modulation of MSCs.Consequently,we hypothesize that MSCs-EVs hold promise as a potential therapy for AP-induced cardiac injury.This paper aims to discuss this topic.However,additional research is essential to comprehensively elucidate the underlying mechanisms of MSCs-EVs in treating AP-induced cardiac injury,as well as to ascertain their safety and efficacy.展开更多
Chinese cabbage has a high annual demand in China.However,clubroot disease caused by the infection of Plasmodiophora brassicae seriously affects its yield.Transcriptome analysis identified a root meristem growth facto...Chinese cabbage has a high annual demand in China.However,clubroot disease caused by the infection of Plasmodiophora brassicae seriously affects its yield.Transcriptome analysis identified a root meristem growth factor 6(BrRGF6)as significantly up-regulated in Chinese cabbage roots infected with Plasmodiophora brassicae.Quantitative reverse-transcription polymerase chain reaction and in situ hybridization analysis showed higher BrRGF6 expression in susceptible materials than in resistant materials.After Plasmodiophora brassicae infection,BrRGF6 expression was significantly up-regulated,especially in susceptible materials.Gene function analysis showed that the roots of Arabidopsis mutant rgf6 grew faster than the wild-type,and delayed the infection progress of Plasmodiophora brassicae.A Protein,nuclear transcription factor Y subunit C(BrNF-YC),was screened from yeast two-hybrid library of Chinese cabbage induced by Plasmodiophora brassicae,and verified to interact with BrRGF6 by yeast two-hybrid co-transfer.Yeast one-hybrid andβGlucuronidase activity analysis showed that BrNF-YC could directly bind to and strongly activate the promoter of BrRGF6.Transgenic verification showed that BrRGF6 or BrNF-YC silenced Chinese cabbage significantly decreased the expression of BrRGF6,accelerated root development,and reduced incidence of clubroot disease.However,after overexpression of BrRGF6 or BrNF-YC,the phenotype showed a reverse trend.Therefore,BrRGF6 silencing accelerated root growth and enhanced resistance to clubroot disease,which was regulated by BrNF-YC.展开更多
Herpes simplex virus(HSV),a member of the Herpesviridae family,is a signi fi cant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections.Acyclovir(ACV)and related nucleoside anal...Herpes simplex virus(HSV),a member of the Herpesviridae family,is a signi fi cant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections.Acyclovir(ACV)and related nucleoside analogues can successfully treat HSV infections,but the emergence of drug resistance to ACV has created a barrier for the treatment of HSV infections,especially in immunocompromised patients.There is an urgent need to explore new and effective tactics to circumvent drug resistance to HSV.This review summarises the current strategies in the development of new targets(the DNA helicase/primase(H/P)complex),new types of molecules(nature products)and new antiviral mechanisms(lethal mutagenesis of Janus-type nucleosides)to fi ght the drug resistance of HSV.展开更多
AIM: To evaluate the clinical efficacy and safety of acupuncture and moxibustion for the treatment of active Crohn's disease(CD).METHODS: Ninety-two patients were equally and randomly divided into the treatment gr...AIM: To evaluate the clinical efficacy and safety of acupuncture and moxibustion for the treatment of active Crohn's disease(CD).METHODS: Ninety-two patients were equally and randomly divided into the treatment group and received herb-partitioned moxibustion combined with acupuncture, and the control group received wheat bran-partitioned moxibustion combined with superficial acupuncture. The patients received three treatment sessions per week for 12 wk and were followed up for 24 wk. The main outcome was evaluated using the CD Activity Index(CDAI) score, and the secondary outcomes were evaluated using laboratory indicators such as hemoglobin(HGB), C-reactive protein(CRP), erythrocyte sedimentation rate, quality-of-life, endoscopic ratings, and intestinal histology scores.RESULTS: The CDAI scores of both the treatment and control groups were significantly reduced after treatment compared with those measured before treatment. However, the degree of improvement in the treatment group was significantly greater than that of the control group. The improvement in symptoms in patients of the treatment group was sustained at follow-up, whereas that of the control group was not. The overall efficacy of the treatment was significantly greater than that of the control. Both groups demonstrated significant improvements in quality-of-life ratings after treatment, but the improvement was significantly greater in the treatment group than in the control group. In addition, the patients in the treatment group showed significantly increased HGB and significantly decreasedCRP levels and histopathological scores at the end of treatment, whereas the control group did not exhibit significant changes.CONCLUSION: Moxibustion with acupuncture provided significant therapeutic benefits in patients with active CD beyond the placebo effect and is therefore an effective and safe treatment for active CD.展开更多
文摘Rickettsia felis is an exclusively cytozoic Gram-negative prokaryote with cat fleas as the major vectors.[1]As early as 1918,Rickettsia felis was detected in cat fleas in Europe and named Rickettsia ctenocephali.[2]Symptoms of fever,malaise,headache,maculopapular rash and eschar are observed in patients with Rickettsia felis infection.
基金Supported by the Capital Fund Project for Clinical Diagnosis and Treatment Technology Research and Translational Application,No.Z201100005520091and Beijing Traditional Chinese Medicine Science and Technology Development Fund Project,No.JJ-2020-67.
文摘BACKGROUND Total knee arthroplasty(TKA)is a mature procedure recommended for correcting knee osteoarthritis deformity,relieving pain,and restoring normal biomechanics.Although TKA is a successful and cost-effective procedure,patient dissatisfaction is as high as 50%.Knee pain after TKA is a significant cause of patient dissatisfaction;the most common location for residual pain is the anterior region.Between 4%and 40%of patients have anterior knee pain(AKP).AIM To investigate the effect of various TKA procedures on postoperative AKP.METHODS We searched PubMed,EMBASE,and Cochrane from January 2000 to September 2022.Randomized controlled trials with one intervention in the experimental group and no corresponding intervention(or other interventions)in the control group were collected.Two researchers independently read the title and abstract of the studies,preliminarily screened the articles,and read the full text in detail according to the selection criteria.Conflicts were resolved by consultation with a third researcher.And relevant data from the included studies were extracted and analyzed using Review Manager 5.4 software.RESULTS There were 25 randomized controlled trials;13 were comparative studies with or without patellar resurfacing.The meta-analysis showed no significant difference between the experimental and control groups(P=0.61).Six studies were comparative studies of circumpatellar denervation vs non-denervation,divided into three subgroups for meta-analysis.The two-subgroup meta-analysis showed no significant difference between the experimental and the control groups(P=0.31,P=0.50).One subgroup meta-analysis showed a significant difference between the experimental and control groups(P=0.001).Two studies compared fixed-bearing TKA and mobile-bearing TKA;the results meta-analysis showed no significant difference between the experimental and control groups(P=0.630).Two studies compared lateral retinacular release vs non-release;the meta-analysis showed a significant difference between the experimental and control groups(P=0.002);two other studies compared other factors.CONCLUSION Patellar resurfacing,mobile-bearing TKA,and fixed-bearing TKA do not reduce the incidence of AKP.Lateral retinacular release can reduce AKP;however,whether circumpatellar denervation can reduce AKP is controversial.
基金Supported by the Natural Science Basic Research Program of Shaanxi Province,China,No.2021JM-284Health Research Projects of Shaanxi Province,China,No.2021A010Science and Technology Planning Project of Xi’an,China,No.22YXYJ0111.
文摘Acute pancreatitis(AP)often leads to a high incidence of cardiac injury,posing significant challenges in the treatment of severe AP and contributing to increased mortality rates.Mesenchymal stem cells(MSCs)release bioactive molecules that participate in various inflammatory diseases.Similarly,extracellular vesicles(EVs)secreted by MSCs have garnered extensive attention due to their comparable anti-inflammatory effects to MSCs and their potential to avoid risks associated with cell transplantation.Recently,the therapeutic potential of MSCs-EVs in various inflammatory diseases,including sepsis and AP,has gained increasing recognition.Although preclinical research on the utilization of MSCs-EVs in AP-induced cardiac injury is limited,several studies have demonstrated the positive effects of MSCs-EVs in regulating inflammation and immunity in sepsis-induced cardiac injury and cardiovascular diseases.Furthermore,clinical studies have been conducted on the therapeutic application of MSCs-EVs for some other diseases,wherein the contents of these EVs could be deliberately modified through prior modulation of MSCs.Consequently,we hypothesize that MSCs-EVs hold promise as a potential therapy for AP-induced cardiac injury.This paper aims to discuss this topic.However,additional research is essential to comprehensively elucidate the underlying mechanisms of MSCs-EVs in treating AP-induced cardiac injury,as well as to ascertain their safety and efficacy.
基金supported by grants from the National Natural Science Foundation of China[grant number 31972412,32272717].
文摘Chinese cabbage has a high annual demand in China.However,clubroot disease caused by the infection of Plasmodiophora brassicae seriously affects its yield.Transcriptome analysis identified a root meristem growth factor 6(BrRGF6)as significantly up-regulated in Chinese cabbage roots infected with Plasmodiophora brassicae.Quantitative reverse-transcription polymerase chain reaction and in situ hybridization analysis showed higher BrRGF6 expression in susceptible materials than in resistant materials.After Plasmodiophora brassicae infection,BrRGF6 expression was significantly up-regulated,especially in susceptible materials.Gene function analysis showed that the roots of Arabidopsis mutant rgf6 grew faster than the wild-type,and delayed the infection progress of Plasmodiophora brassicae.A Protein,nuclear transcription factor Y subunit C(BrNF-YC),was screened from yeast two-hybrid library of Chinese cabbage induced by Plasmodiophora brassicae,and verified to interact with BrRGF6 by yeast two-hybrid co-transfer.Yeast one-hybrid andβGlucuronidase activity analysis showed that BrNF-YC could directly bind to and strongly activate the promoter of BrRGF6.Transgenic verification showed that BrRGF6 or BrNF-YC silenced Chinese cabbage significantly decreased the expression of BrRGF6,accelerated root development,and reduced incidence of clubroot disease.However,after overexpression of BrRGF6 or BrNF-YC,the phenotype showed a reverse trend.Therefore,BrRGF6 silencing accelerated root growth and enhanced resistance to clubroot disease,which was regulated by BrNF-YC.
基金the National Natural Science Foundations of China(document no.:81321002,81500860,81300888)a grant from 111 Project of Ministry of Education,China,for fi nancial support
文摘Herpes simplex virus(HSV),a member of the Herpesviridae family,is a signi fi cant human pathogen that results in mucocutaneous lesions in the oral cavity or genital infections.Acyclovir(ACV)and related nucleoside analogues can successfully treat HSV infections,but the emergence of drug resistance to ACV has created a barrier for the treatment of HSV infections,especially in immunocompromised patients.There is an urgent need to explore new and effective tactics to circumvent drug resistance to HSV.This review summarises the current strategies in the development of new targets(the DNA helicase/primase(H/P)complex),new types of molecules(nature products)and new antiviral mechanisms(lethal mutagenesis of Janus-type nucleosides)to fi ght the drug resistance of HSV.
基金Supported by Shanghai Municipal Health Bureau,No.20124028The Ministry of Education Program for New Century Excellent Talents,No.NCET-13-0907+1 种基金Shanghai Municipal Science and Technology Commission,No.13ZR1439400Shanghai Top Clinical Medical Center of Acupuncture,Moxibustion and Tuina
文摘AIM: To evaluate the clinical efficacy and safety of acupuncture and moxibustion for the treatment of active Crohn's disease(CD).METHODS: Ninety-two patients were equally and randomly divided into the treatment group and received herb-partitioned moxibustion combined with acupuncture, and the control group received wheat bran-partitioned moxibustion combined with superficial acupuncture. The patients received three treatment sessions per week for 12 wk and were followed up for 24 wk. The main outcome was evaluated using the CD Activity Index(CDAI) score, and the secondary outcomes were evaluated using laboratory indicators such as hemoglobin(HGB), C-reactive protein(CRP), erythrocyte sedimentation rate, quality-of-life, endoscopic ratings, and intestinal histology scores.RESULTS: The CDAI scores of both the treatment and control groups were significantly reduced after treatment compared with those measured before treatment. However, the degree of improvement in the treatment group was significantly greater than that of the control group. The improvement in symptoms in patients of the treatment group was sustained at follow-up, whereas that of the control group was not. The overall efficacy of the treatment was significantly greater than that of the control. Both groups demonstrated significant improvements in quality-of-life ratings after treatment, but the improvement was significantly greater in the treatment group than in the control group. In addition, the patients in the treatment group showed significantly increased HGB and significantly decreasedCRP levels and histopathological scores at the end of treatment, whereas the control group did not exhibit significant changes.CONCLUSION: Moxibustion with acupuncture provided significant therapeutic benefits in patients with active CD beyond the placebo effect and is therefore an effective and safe treatment for active CD.
