BACKGROUND Non-surgical methods such as percutaneous drainage are crucial for the treatment of patients with severe acute pancreatitis(SAP).However,there is still an ongoing debate regarding the optimal timing for abd...BACKGROUND Non-surgical methods such as percutaneous drainage are crucial for the treatment of patients with severe acute pancreatitis(SAP).However,there is still an ongoing debate regarding the optimal timing for abdominal paracentesis catheter place-ment and drainage.AIM To explore the influence of different timing for abdominal paracentesis catheter placement and drainage in SAP complicated by intra-abdominal fluid accumu-lation.METHODS Using a retrospective approach,184 cases of SAP complicated by intra-abdominal fluid accumulation were enrolled and categorized into three groups based on the timing of catheter placement:group A(catheter placement within 2 d of symptom onset,n=89),group B(catheter placement between days 3 and 5 after symptom onset,n=55),and group C(catheter placement between days 6 and 7 after symptom onset,n=40).The differences in progression rate,mortality rate,and the number of cases with organ dysfunction were compared among the three groups.RESULTS The progression rate of group A was significantly lower than those in groups B and groups C(2.25%vs 21.82%and 32.50%,P<0.05).Further,the proportion of patients with at least one organ dysfunction in group A was significantly lower than those in groups B and groups C(41.57%vs 70.91%and 75.00%,P<0.05).The mortality rates in group A,group B,and group C were similar(P>0.05).At postoperative day 3,the levels of C-reactive protein(55.41±19.32 mg/L vs 82.25±20.41 mg/L and 88.65±19.14 mg/L,P<0.05),procalcitonin(1.36±0.51 ng/mL vs 3.20±0.97 ng/mL and 3.41±0.98 ng/mL,P<0.05),tumor necrosis factor-alpha(15.12±6.63 pg/L vs 22.26±9.96 pg/L and 23.39±9.12 pg/L,P<0.05),interleukin-6(332.14±90.16 ng/L vs 412.20±88.50 ng/L and 420.08±87.65ng/L,P<0.05),interleukin-8(415.54±68.43 ng/L vs 505.80±66.90 ng/L and 510.43±68.23ng/L,P<0.05)and serum amyloid A(270.06±78.49 mg/L vs 344.41±81.96 mg/L and 350.60±80.42 mg/L,P<0.05)were significantly lower in group A compared to those in groups B and group C.The length of hospital stay in group A was significantly lower than those in groups B and group C(24.50±4.16 d vs 35.54±6.62 d and 38.89±7.10 d,P<0.05).The hospitalization expenses in group A were also significantly lower than those in groups B and groups C[2.70(1.20,3.55)ten-thousand-yuan vs 5.50(2.98,7.12)ten-thousand-yuan and 6.00(3.10,8.05)ten-thousand-yuan,P<0.05).The incidence of complications in group A was markedly lower than that in group C(5.62%vs 25.00%,P<0.05),and similar to group B(P>0.05).CONCLUSION Percutaneous catheter drainage for the treatment of SAP complicated by intra-abdominal fluid accumulation is most effective when performed within 2 d of onset.展开更多
Objective:To establish a rat model of diabetic ulcer infected by Staphylococcus aureus and Escherichia coli, and provide a suitable animal model for experimental research of diabetic ulcer. Methods: Forty male SD rats...Objective:To establish a rat model of diabetic ulcer infected by Staphylococcus aureus and Escherichia coli, and provide a suitable animal model for experimental research of diabetic ulcer. Methods: Forty male SD rats were randomly divided into five groups: general ulcer group, diabetic ulcer group, diabetic ulcer treatment group, model group, and model treatment group. After modeling, take local skin tissue for HE staining.Detection of high-sensitivity C-reactive protein, fibroblast growth factor, tumor necrosis factor-α, advanced glycosylation products, interleukin-1β, interleukin-6, and nitric oxide in rat serum by enzyme-linked immunosorbent Level, and record the wound healing time, and evaluate the stability and practicability of the model. Results:The healing time of ulcer in the model group was significantly delayed compared with other groups (p <0.001). The serum H-CRP levels in the model group and the model treatment group were higher than those in the common ulcer group, the diabetic ulcer group, and the diabetic ulcer treatment group (p <0.05). After healing, the levels of H-CRP, bFGF, AGEs, TNF-α, IL-1β, IL-6, and NO in the serum of the diabetic ulcer group were higher than those in the common ulcer group (all p <0.05);The levels of TNF-α, IL-1β, IL-6, and NO were higher than those in other groups (all p <0.05);HCRP, AGEs, TNF-α, IL-1β, IL-6, and NO levels in the serum of the model treatment group They were all higher than those in diabetic ulcer treatment group (all p <0.05). Conclusion: This model is in line with the local environment of infectious diabetic ulcers and provides a reliable tool for the study of diabetic infections.展开更多
In order to investigate the effect of erbium (Er) on the microstructure of orthorhombic Ti2AlNb-based alloys, four testing alloys were prepared by adding different contents of the rare earth metal Er to Ti-22Al-25Nb a...In order to investigate the effect of erbium (Er) on the microstructure of orthorhombic Ti2AlNb-based alloys, four testing alloys were prepared by adding different contents of the rare earth metal Er to Ti-22Al-25Nb alloy and optical microscopy, X-ray diffraction, scanning electron microscopy, electron probe microanalysis, energy-dispersive spectrometry, and transmission electron microscopy were used. The results show that the addition of Er is capable of grain refining and the refinement effect is more obvious with increasing content of Er. The Er2O3 dispersoids formed by internal oxidation and Al3Er compound particles are observed in Er-doped alloys and the number of Er precipitates is increased with increasing Er addition. It is likely that the solubility of Er in the Ti2AlNb alloy is very low and Er precipitates tend to segregate at grain boundaries, which together with the surface activity of rare earth elements is supposed to decrease the prior B2 grain size of Ti-22Al-25Nb alloy effectively.展开更多
BACKGROUND The severity of acute pancreatitis in pregnancy(APIP)is correlated with higher risks of maternal and fetal death.AIM To develop a nomogram that could predict moderately severe and severe acute pancreatitis ...BACKGROUND The severity of acute pancreatitis in pregnancy(APIP)is correlated with higher risks of maternal and fetal death.AIM To develop a nomogram that could predict moderately severe and severe acute pancreatitis in pregnancy(MSIP).METHODS Patients with APIP admitted to West China Hospital between January 2012 and December 2018 were included in this study.They were divided into mild acute pancreatitis in pregnancy(MAIP)and MSIP.Characteristic parameters and laboratory results were collected.The training set and test set were randomly divided at a ratio of 7:3.Least absolute shrinkage and selection operator regression was used to select potential prognostic factors.A nomogram was developed by logistic regression.A random forest model was used to validate the stability of the prediction factors.Receiver operating characteristic curves and calibration curves were used to evaluate the model’s predictive performance.RESULTS A total of 190 patients were included in this study.A total of 134 patients(70.5%)and 56 patients(29.5%)were classified as having MAIP and MSIP,respectively.Four independent predictors(lactate dehydrogenase,triglyceride,cholesterol,and albumin levels)were identified for MSIP.A nomogram prediction model based on these factors was established.The model had areas under the curve of 0.865 and 0.853 in the training and validation sets,respectively.The calibration curves showed that the nomogram has a good consistency.CONCLUSION A nomogram including lactate dehydrogenase,triglyceride,cholesterol,and albumin levels as independent predictors was built with good performance for MSIP prediction.展开更多
BACKGROUND Deep vein thrombosis(DVT)may cause pulmonary embolus,leading to late deaths.The systemic inflammatory and hypercoagulable state of moderate and severe acute pancreatitis(non-mild acute pancreatitis,NMAP)pat...BACKGROUND Deep vein thrombosis(DVT)may cause pulmonary embolus,leading to late deaths.The systemic inflammatory and hypercoagulable state of moderate and severe acute pancreatitis(non-mild acute pancreatitis,NMAP)patients may contribute to the development of venous thromboembolism.Accurate prediction of DVT is conducive to clinical decisions.AIM To develop and validate a potential new prediction nomogram model for the occurrence of DVT in NMAP.METHODS NMAP patient admission between 2013.1.1 and 2018.12.31 at the West China Hospital of Sichuan University was collected.A total of 220 patients formed the training set for nomogram development,and a validation set was constructed using bootstrapping with 100 resamplings.Univariate and multivariate logistic regression analyses were used to estimate independent risk factors associated with DVT.The independent risk factors were included in the nomogram.The accuracy and utility of the nomogram were evaluated by calibration curve and decision curve analysis,respectively.RESULTS A total of 220 NMAP patients over 60 years old were enrolled for this analysis.DVT was detected in 80(36.4%)patients.The final nomogram included age,sex,surgery times,D-dimer,neutrophils,any organ failure,blood culture,and classification.This model achieved good concordance indexes of 0.827(95%CI:0.769-0.885)and 0.803(95%CI:0.743-0.860)in the training and validation sets,respectively.CONCLUSION We developed and validated a prediction nomogram model for DVT in older patients with NMAP.This may help guide doctors in making sound decisions regarding the administration of DVT prophylaxis.展开更多
Objective:To explore the mechanism of Radix Astragali-Lithospermum Erythrorhizon on the treatment of diabetic ulcer through the method of network pharmacology.Methods:This study included 32 compounds and 81 key target...Objective:To explore the mechanism of Radix Astragali-Lithospermum Erythrorhizon on the treatment of diabetic ulcer through the method of network pharmacology.Methods:This study included 32 compounds and 81 key targets.100 GO functional items and 116 KEGG signal pathways were obtained by enrichment analysis.Quercetin,kaempferol,isorhamnetin,mononetin,sitosterol,ivy sapogenin and other components of astragalus-purple herb play a key role in the targets of interleukin-6,cystatin 3,vascular endothelial growth factor,epidermal growth factor receptor and mitogen-activated protein kinase 8 in diabetic ulcer,and are mainly concentrated in AGE-RAGE,TNF and other signal pathways.Results:There were 32 compounds and 81 key targets.100 GO functional items and 116 KEGG signal pathways were obtained by enrichment analysis.Quercetin,kaempferol,isorhamnetin,mononetin,sitosterol,ivy sapogenin and other components of astragalus-purple herb play a key role in the targets of interleukin-6,cystatin 3,vascular endothelial growth factor,epidermal growth factor receptor and mitogen-activated protein kinase 8 in diabetic ulcer,and are mainly concentrated in AGE-RAGE,TNF and other signal pathways.Conclusion:Radix Astragali-Lithospermum Erythrorhizon may play the role of inhibiting inflammation,anti-apoptosis,promoting cell proliferation,angiogenesis and immune regulation through multi-components and multitargets,and play a role in the treatment of diabetic ulcer.展开更多
In order to investigate the boron removal effect in slag refining process,intermediate frequency furnace was used to purify boron in SiO2-CaO-Na3 AlF6-CaSiO3 slag system at 1,550℃,and back propagation(BP)neural netwo...In order to investigate the boron removal effect in slag refining process,intermediate frequency furnace was used to purify boron in SiO2-CaO-Na3 AlF6-CaSiO3 slag system at 1,550℃,and back propagation(BP)neural network was used to model the relationship between slag compositions and boron content in SiO2-CaO-Na3 AlF6-CaSiO3 slag system.The BP neural network predicted error is below 2.38%.The prediction results show that the slag composition has a significant influence on boron removal.Increasing the basicity of slag by adding CaO or Na3 AlF6 to CaSiO3-based slag could contribute to the boron removal,and the addition of Na3 AlF6 has a better removal effect in comparison with the addition of CaO.The oxidizing characteristic of CaSiO3 results in the ineffective removal with the addition of SiO2.The increase of oxygen potential(pO2)in the CaO-Na3 AlF6-CaSiO3 slag system by varying the SiO2 proportion can also contribute to the boron removal in silicon ingot.The best slag composition to remove boron was predicted by BP neural network using genetic algorithm(GA).The predicted results show that the mass fraction of boron in silicon reduces from 14.0000×10-6 to0.4366×10-6 after slag melting using 23.12%SiO2-10.44%CaO-16.83%Na3 AlF6-49.61%CaSiO3 slag system,close to the experimental boron content in silicon which is below 0.5×10-6.展开更多
BACKGROUND Our study investigated the role of FAM53B in regulating macrophage M2 polarization and its potential mechanisms in promoting pancreatic ductal adenocarcinoma(PDAC)metastasis.AIM To further investigate the r...BACKGROUND Our study investigated the role of FAM53B in regulating macrophage M2 polarization and its potential mechanisms in promoting pancreatic ductal adenocarcinoma(PDAC)metastasis.AIM To further investigate the role of FAM53B in regulating macrophage M2 polarization and its potential mechanism in promoting PDAC metastasis.Our goal is to determine how FAM53B affects macrophage M2 polarization and to define its underlying mechanism in PDAC metastasis.METHODS Cell culture and various experiments,including protein analysis,immunohisto-chemistry,and animal model experiments,were conducted.We compared FAM53B expression between PDAC tissues and healthy tissues and assessed the correlation of FAM53B expression with clinical features.Our study analyzed the role of FAM53B in macrophage M2 polarization in vitro by examining the expression of relevant markers.Finally,we used a murine model to study the role of FAM53B in PDAC metastasis and analyzed the potential underlying mechanisms.RESULTS Our research showed that there was a significant increase in FAM53B levels in PDAC tissues,which was linked to adverse tumor features.Experimental findings indicated that FAM53B can enhance macrophage M2 polarization,leading to increased anti-inflammatory factor release.The results from the mouse model further supported the role of FAM53B in PDAC metastasis,as blocking FAM53B prevented tumor cell invasion and metastasis.CONCLUSION FAM53B promotes PDAC metastasis by regulating macrophage M2 polarization.This discovery could lead to the development of new strategies for treating PDAC.For example,interfering with the FAM53B signaling pathway may prevent cancer spread.Our research findings also provide important information for expanding our understanding of PDAC pathogenesis.展开更多
To the Editor:Hyperuricemia,the second most common metabolic disease after diabetes mellitus in China,is characterized by the elevated serum uric concentration which is due to the overactive uric acid production or un...To the Editor:Hyperuricemia,the second most common metabolic disease after diabetes mellitus in China,is characterized by the elevated serum uric concentration which is due to the overactive uric acid production or underexcretion.[1]Recent study supported the notion that hyperuricemia is implicated in renal tubular injury,subsequent tubulointerstitial fibrosis,and finally gouty nephropathy.[2]However,the lack of a suitable and stable mouse model to study hyperuricemia and gout nephropathy delays and limits the development of mechanism research and new drugs.Further understanding of the pathogenesis of hyperuricemia and gouty nephropathy has been largely hampered by a lack of preclinical animal models that reliably capture the characteristic phenotypes of hyperuricemia and gouty nephropathy.In this study,we aimed to develop a novel mouse model for hyperuricemia and gouty nephropathy.展开更多
Platelet-derived growth subunit A(PDGFA)plays critical roles in development of glioblastoma(GBM)with substantial evidence from TCGA database analyses and in vivo mouse models.So far,only platelet-derived growth recept...Platelet-derived growth subunit A(PDGFA)plays critical roles in development of glioblastoma(GBM)with substantial evidence from TCGA database analyses and in vivo mouse models.So far,only platelet-derived growth receptor a(PDGFRA)has been identified as receptor for PDGFA.However,PDGFA and PDGFRA are categorized into different molecular subtypes of GBM in TCGA_GBM database.展开更多
文摘BACKGROUND Non-surgical methods such as percutaneous drainage are crucial for the treatment of patients with severe acute pancreatitis(SAP).However,there is still an ongoing debate regarding the optimal timing for abdominal paracentesis catheter place-ment and drainage.AIM To explore the influence of different timing for abdominal paracentesis catheter placement and drainage in SAP complicated by intra-abdominal fluid accumu-lation.METHODS Using a retrospective approach,184 cases of SAP complicated by intra-abdominal fluid accumulation were enrolled and categorized into three groups based on the timing of catheter placement:group A(catheter placement within 2 d of symptom onset,n=89),group B(catheter placement between days 3 and 5 after symptom onset,n=55),and group C(catheter placement between days 6 and 7 after symptom onset,n=40).The differences in progression rate,mortality rate,and the number of cases with organ dysfunction were compared among the three groups.RESULTS The progression rate of group A was significantly lower than those in groups B and groups C(2.25%vs 21.82%and 32.50%,P<0.05).Further,the proportion of patients with at least one organ dysfunction in group A was significantly lower than those in groups B and groups C(41.57%vs 70.91%and 75.00%,P<0.05).The mortality rates in group A,group B,and group C were similar(P>0.05).At postoperative day 3,the levels of C-reactive protein(55.41±19.32 mg/L vs 82.25±20.41 mg/L and 88.65±19.14 mg/L,P<0.05),procalcitonin(1.36±0.51 ng/mL vs 3.20±0.97 ng/mL and 3.41±0.98 ng/mL,P<0.05),tumor necrosis factor-alpha(15.12±6.63 pg/L vs 22.26±9.96 pg/L and 23.39±9.12 pg/L,P<0.05),interleukin-6(332.14±90.16 ng/L vs 412.20±88.50 ng/L and 420.08±87.65ng/L,P<0.05),interleukin-8(415.54±68.43 ng/L vs 505.80±66.90 ng/L and 510.43±68.23ng/L,P<0.05)and serum amyloid A(270.06±78.49 mg/L vs 344.41±81.96 mg/L and 350.60±80.42 mg/L,P<0.05)were significantly lower in group A compared to those in groups B and group C.The length of hospital stay in group A was significantly lower than those in groups B and group C(24.50±4.16 d vs 35.54±6.62 d and 38.89±7.10 d,P<0.05).The hospitalization expenses in group A were also significantly lower than those in groups B and groups C[2.70(1.20,3.55)ten-thousand-yuan vs 5.50(2.98,7.12)ten-thousand-yuan and 6.00(3.10,8.05)ten-thousand-yuan,P<0.05).The incidence of complications in group A was markedly lower than that in group C(5.62%vs 25.00%,P<0.05),and similar to group B(P>0.05).CONCLUSION Percutaneous catheter drainage for the treatment of SAP complicated by intra-abdominal fluid accumulation is most effective when performed within 2 d of onset.
基金NSFC(No.81774310)National natural science foundation of China youth program(No.81804095)Shanghai TCM health service collaborative innovation center project(No.ZYJKFW201701002).
文摘Objective:To establish a rat model of diabetic ulcer infected by Staphylococcus aureus and Escherichia coli, and provide a suitable animal model for experimental research of diabetic ulcer. Methods: Forty male SD rats were randomly divided into five groups: general ulcer group, diabetic ulcer group, diabetic ulcer treatment group, model group, and model treatment group. After modeling, take local skin tissue for HE staining.Detection of high-sensitivity C-reactive protein, fibroblast growth factor, tumor necrosis factor-α, advanced glycosylation products, interleukin-1β, interleukin-6, and nitric oxide in rat serum by enzyme-linked immunosorbent Level, and record the wound healing time, and evaluate the stability and practicability of the model. Results:The healing time of ulcer in the model group was significantly delayed compared with other groups (p <0.001). The serum H-CRP levels in the model group and the model treatment group were higher than those in the common ulcer group, the diabetic ulcer group, and the diabetic ulcer treatment group (p <0.05). After healing, the levels of H-CRP, bFGF, AGEs, TNF-α, IL-1β, IL-6, and NO in the serum of the diabetic ulcer group were higher than those in the common ulcer group (all p <0.05);The levels of TNF-α, IL-1β, IL-6, and NO were higher than those in other groups (all p <0.05);HCRP, AGEs, TNF-α, IL-1β, IL-6, and NO levels in the serum of the model treatment group They were all higher than those in diabetic ulcer treatment group (all p <0.05). Conclusion: This model is in line with the local environment of infectious diabetic ulcers and provides a reliable tool for the study of diabetic infections.
基金financially supported by the Science and Technology Ministry of China to the National High Technology "863" Project (No. 2012AA062302)
文摘In order to investigate the effect of erbium (Er) on the microstructure of orthorhombic Ti2AlNb-based alloys, four testing alloys were prepared by adding different contents of the rare earth metal Er to Ti-22Al-25Nb alloy and optical microscopy, X-ray diffraction, scanning electron microscopy, electron probe microanalysis, energy-dispersive spectrometry, and transmission electron microscopy were used. The results show that the addition of Er is capable of grain refining and the refinement effect is more obvious with increasing content of Er. The Er2O3 dispersoids formed by internal oxidation and Al3Er compound particles are observed in Er-doped alloys and the number of Er precipitates is increased with increasing Er addition. It is likely that the solubility of Er in the Ti2AlNb alloy is very low and Er precipitates tend to segregate at grain boundaries, which together with the surface activity of rare earth elements is supposed to decrease the prior B2 grain size of Ti-22Al-25Nb alloy effectively.
基金Supported by the 1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University No.ZYGD20006 and ZYJC18027。
文摘BACKGROUND The severity of acute pancreatitis in pregnancy(APIP)is correlated with higher risks of maternal and fetal death.AIM To develop a nomogram that could predict moderately severe and severe acute pancreatitis in pregnancy(MSIP).METHODS Patients with APIP admitted to West China Hospital between January 2012 and December 2018 were included in this study.They were divided into mild acute pancreatitis in pregnancy(MAIP)and MSIP.Characteristic parameters and laboratory results were collected.The training set and test set were randomly divided at a ratio of 7:3.Least absolute shrinkage and selection operator regression was used to select potential prognostic factors.A nomogram was developed by logistic regression.A random forest model was used to validate the stability of the prediction factors.Receiver operating characteristic curves and calibration curves were used to evaluate the model’s predictive performance.RESULTS A total of 190 patients were included in this study.A total of 134 patients(70.5%)and 56 patients(29.5%)were classified as having MAIP and MSIP,respectively.Four independent predictors(lactate dehydrogenase,triglyceride,cholesterol,and albumin levels)were identified for MSIP.A nomogram prediction model based on these factors was established.The model had areas under the curve of 0.865 and 0.853 in the training and validation sets,respectively.The calibration curves showed that the nomogram has a good consistency.CONCLUSION A nomogram including lactate dehydrogenase,triglyceride,cholesterol,and albumin levels as independent predictors was built with good performance for MSIP prediction.
基金Supported by The Sichuan Provincial Department of Science and Technology Supporting Project,No.2018SZ03811.3.5 project for disciplines of excellence,West China Hospital,Sichuan University,No.ZYJC18027.
文摘BACKGROUND Deep vein thrombosis(DVT)may cause pulmonary embolus,leading to late deaths.The systemic inflammatory and hypercoagulable state of moderate and severe acute pancreatitis(non-mild acute pancreatitis,NMAP)patients may contribute to the development of venous thromboembolism.Accurate prediction of DVT is conducive to clinical decisions.AIM To develop and validate a potential new prediction nomogram model for the occurrence of DVT in NMAP.METHODS NMAP patient admission between 2013.1.1 and 2018.12.31 at the West China Hospital of Sichuan University was collected.A total of 220 patients formed the training set for nomogram development,and a validation set was constructed using bootstrapping with 100 resamplings.Univariate and multivariate logistic regression analyses were used to estimate independent risk factors associated with DVT.The independent risk factors were included in the nomogram.The accuracy and utility of the nomogram were evaluated by calibration curve and decision curve analysis,respectively.RESULTS A total of 220 NMAP patients over 60 years old were enrolled for this analysis.DVT was detected in 80(36.4%)patients.The final nomogram included age,sex,surgery times,D-dimer,neutrophils,any organ failure,blood culture,and classification.This model achieved good concordance indexes of 0.827(95%CI:0.769-0.885)and 0.803(95%CI:0.743-0.860)in the training and validation sets,respectively.CONCLUSION We developed and validated a prediction nomogram model for DVT in older patients with NMAP.This may help guide doctors in making sound decisions regarding the administration of DVT prophylaxis.
基金General project of national natural science foundation of China(No.81774310)Youth project of national natural science foundation of China(No.81804095)。
文摘Objective:To explore the mechanism of Radix Astragali-Lithospermum Erythrorhizon on the treatment of diabetic ulcer through the method of network pharmacology.Methods:This study included 32 compounds and 81 key targets.100 GO functional items and 116 KEGG signal pathways were obtained by enrichment analysis.Quercetin,kaempferol,isorhamnetin,mononetin,sitosterol,ivy sapogenin and other components of astragalus-purple herb play a key role in the targets of interleukin-6,cystatin 3,vascular endothelial growth factor,epidermal growth factor receptor and mitogen-activated protein kinase 8 in diabetic ulcer,and are mainly concentrated in AGE-RAGE,TNF and other signal pathways.Results:There were 32 compounds and 81 key targets.100 GO functional items and 116 KEGG signal pathways were obtained by enrichment analysis.Quercetin,kaempferol,isorhamnetin,mononetin,sitosterol,ivy sapogenin and other components of astragalus-purple herb play a key role in the targets of interleukin-6,cystatin 3,vascular endothelial growth factor,epidermal growth factor receptor and mitogen-activated protein kinase 8 in diabetic ulcer,and are mainly concentrated in AGE-RAGE,TNF and other signal pathways.Conclusion:Radix Astragali-Lithospermum Erythrorhizon may play the role of inhibiting inflammation,anti-apoptosis,promoting cell proliferation,angiogenesis and immune regulation through multi-components and multitargets,and play a role in the treatment of diabetic ulcer.
基金financially supported by the National High Technology Research and Development Program of China (No.2012AA062302)。
文摘In order to investigate the boron removal effect in slag refining process,intermediate frequency furnace was used to purify boron in SiO2-CaO-Na3 AlF6-CaSiO3 slag system at 1,550℃,and back propagation(BP)neural network was used to model the relationship between slag compositions and boron content in SiO2-CaO-Na3 AlF6-CaSiO3 slag system.The BP neural network predicted error is below 2.38%.The prediction results show that the slag composition has a significant influence on boron removal.Increasing the basicity of slag by adding CaO or Na3 AlF6 to CaSiO3-based slag could contribute to the boron removal,and the addition of Na3 AlF6 has a better removal effect in comparison with the addition of CaO.The oxidizing characteristic of CaSiO3 results in the ineffective removal with the addition of SiO2.The increase of oxygen potential(pO2)in the CaO-Na3 AlF6-CaSiO3 slag system by varying the SiO2 proportion can also contribute to the boron removal in silicon ingot.The best slag composition to remove boron was predicted by BP neural network using genetic algorithm(GA).The predicted results show that the mass fraction of boron in silicon reduces from 14.0000×10-6 to0.4366×10-6 after slag melting using 23.12%SiO2-10.44%CaO-16.83%Na3 AlF6-49.61%CaSiO3 slag system,close to the experimental boron content in silicon which is below 0.5×10-6.
文摘BACKGROUND Our study investigated the role of FAM53B in regulating macrophage M2 polarization and its potential mechanisms in promoting pancreatic ductal adenocarcinoma(PDAC)metastasis.AIM To further investigate the role of FAM53B in regulating macrophage M2 polarization and its potential mechanism in promoting PDAC metastasis.Our goal is to determine how FAM53B affects macrophage M2 polarization and to define its underlying mechanism in PDAC metastasis.METHODS Cell culture and various experiments,including protein analysis,immunohisto-chemistry,and animal model experiments,were conducted.We compared FAM53B expression between PDAC tissues and healthy tissues and assessed the correlation of FAM53B expression with clinical features.Our study analyzed the role of FAM53B in macrophage M2 polarization in vitro by examining the expression of relevant markers.Finally,we used a murine model to study the role of FAM53B in PDAC metastasis and analyzed the potential underlying mechanisms.RESULTS Our research showed that there was a significant increase in FAM53B levels in PDAC tissues,which was linked to adverse tumor features.Experimental findings indicated that FAM53B can enhance macrophage M2 polarization,leading to increased anti-inflammatory factor release.The results from the mouse model further supported the role of FAM53B in PDAC metastasis,as blocking FAM53B prevented tumor cell invasion and metastasis.CONCLUSION FAM53B promotes PDAC metastasis by regulating macrophage M2 polarization.This discovery could lead to the development of new strategies for treating PDAC.For example,interfering with the FAM53B signaling pathway may prevent cancer spread.Our research findings also provide important information for expanding our understanding of PDAC pathogenesis.
基金Supported by grants from the Science and Technology Planning Project of Guangdong Province(No.2017A020211007),Chinathe Key Project of Natural Science Foundation of Guangdong Province(No.2016A030311014),China+2 种基金the National Natural Science Foundation of China(No.81870420)Special Project on the Integration of Industry,Education and Research of Guangdong Province(No.2014B090902002)Science and Technological Program for Dongguan’s Social Development(No.2016108101043).
文摘To the Editor:Hyperuricemia,the second most common metabolic disease after diabetes mellitus in China,is characterized by the elevated serum uric concentration which is due to the overactive uric acid production or underexcretion.[1]Recent study supported the notion that hyperuricemia is implicated in renal tubular injury,subsequent tubulointerstitial fibrosis,and finally gouty nephropathy.[2]However,the lack of a suitable and stable mouse model to study hyperuricemia and gout nephropathy delays and limits the development of mechanism research and new drugs.Further understanding of the pathogenesis of hyperuricemia and gouty nephropathy has been largely hampered by a lack of preclinical animal models that reliably capture the characteristic phenotypes of hyperuricemia and gouty nephropathy.In this study,we aimed to develop a novel mouse model for hyperuricemia and gouty nephropathy.
基金This work was supported by:The National Key Research and Development Program of China(2017YFC1309004 to Y.W.)The National Natural Science Foundation of China(81972365 to Y.W.,81821003 to X.-W.B.,81602006 to F.-L.C.,and 81922056 to Y.S.)Chongqing Academician Program(cstc2019yszx-jcyjX0008 to Y.W.and cstc2018jcyj-yszxX0008 to X.-H.Y.).
文摘Platelet-derived growth subunit A(PDGFA)plays critical roles in development of glioblastoma(GBM)with substantial evidence from TCGA database analyses and in vivo mouse models.So far,only platelet-derived growth receptor a(PDGFRA)has been identified as receptor for PDGFA.However,PDGFA and PDGFRA are categorized into different molecular subtypes of GBM in TCGA_GBM database.
