LBD(lateral organ boundaries)转录因子在植物生长发育和次生代谢调控中起着重要作用。为了发掘大麻LBD基因的功能,该研究在基因组和转录组水平上利用生物信息学手段,对大麻LBD基因家族进行系统鉴定,并对其表达模式进行分析。结果表明...LBD(lateral organ boundaries)转录因子在植物生长发育和次生代谢调控中起着重要作用。为了发掘大麻LBD基因的功能,该研究在基因组和转录组水平上利用生物信息学手段,对大麻LBD基因家族进行系统鉴定,并对其表达模式进行分析。结果表明,大麻LBD基因家族含有32个成员,可分成2大类,7亚族,ClassⅠ分为5个亚族分别是ClassⅠ_a至ClassⅠ_e,ClassⅡ分为2个亚族分别是ClassⅡ_a和ClassⅡ_b;理化性质分析显示,大麻LBD基因家族编码的氨基酸数目为172~356,等电点为4.92~9.43,相对分子质量为18 862.92~40 081.33,大部分成员定位于细胞核中;染色体定位显示32个成员不均一地分布在大麻的10条染色体上;LBD转录因子结构域、基因结构和Motifs相对保守,不同类成员特征相近;基因上游启动子区含有多种植物激素及环境因子相关的顺式作用元件,表明LBD基因的表达可能会受到激素和外界环境因素的诱导;大麻LBD基因在ZYS品种(低四氢大麻酚,高二氢大麻酚)茎、叶、花的表达模式不同,LBD基因家族成员主要在ZYS品种的花和茎中表达,而在叶中表达的成员很少;ClassⅡ成员CsLBD21和CsLBD23在花和茎中表达,CsLBD8和CsLBD18在花、茎和叶中均有表达,这些基因可能参与大麻的生长发育发育进而影响大麻素的生物合成。该研究为后续大麻LBD基因家族的功能研究奠定了基础。展开更多
目的Dof(DNA binding with one finger)基因家族是植物特有转录因子基因家族之一,在植物生长发育和其他非生物胁迫响应中起重要调控作用。本研究以工业大麻基因组中的Dof转录因子作为研究对象,针对Dof基因家族的基因结构、染色体定位、...目的Dof(DNA binding with one finger)基因家族是植物特有转录因子基因家族之一,在植物生长发育和其他非生物胁迫响应中起重要调控作用。本研究以工业大麻基因组中的Dof转录因子作为研究对象,针对Dof基因家族的基因结构、染色体定位、编码蛋白的理化性质及系统发育等方面进行分析。方法本研究通过生物信息学手段对工业大麻Dof基因进行鉴定和分析,使用PlantTFDB、ExPASy、MEME、WoLFPSORT、PlantCare等在线网站及TBTools、MEGA、Clustal、Gendoc等软件进行预测、可视化分析。结果研究发现,大麻中含有27个Dof基因成员,命名为CsDof1~CsDof27;染色体定位分析结果表明,27个Dof基因不均匀地分布在9条染色体上。由理化性质分析可知,氨基酸数目在185~512 aa之间,等电点介于4.91~9.22之间,所有Dof基因定位在细胞核中;通过对大麻和拟南芥Dof蛋白的系统发育分析,Dof基因家族可以被分为9个亚组。结论大麻Dof基因具有多个顺式作用元件,转录组数据表达热图显示部分大麻Dof基因具有茎和花组织表达特异性,未来研究将重点放在大麻花序中特异性表达的Dof基因上的功能研究。展开更多
Camptotheca acuminata produces camptothecin(CPT),a monoterpene indole alkaloid(MIA)that is widely used in the treatment of lung,colorectal,cervical,and ovarian cancers.Its biosynthesis pathway has attracted significan...Camptotheca acuminata produces camptothecin(CPT),a monoterpene indole alkaloid(MIA)that is widely used in the treatment of lung,colorectal,cervical,and ovarian cancers.Its biosynthesis pathway has attracted significant attention,but the regulation of CPT biosynthesis by the APETALA2/ethylene-responsive factor(AP2/ERF)transcription factors(TFs)remains unclear.In this study,a systematic analysis of the AP2/ERF TFs family in C.acuminata was performed,including phylogeny,gene structure,conserved motifs,and gene expression profiles in different tissues and organs(immature bark,cotyledons,young flower,immature fruit,mature fruit,mature leaf,roots,upper stem,and lower stem)of C.acuminata.A total of 198 AP2/ERF genes were identified and divided into five relatively conserved subfamilies,including AP2(26 genes),DREB(61 genes),ERF(92 genes),RAV(18 genes),and Soloist(one gene).The combination of gene expression patterns in different C.acuminata tissues and organs,the phylogenetic tree,the co-expression analysis with biosynthetic genes,and the analysis of promoter sequences of key enzymes genes involved in CPT biosynthesis pathways revealed that eight AP2/ERF TFs in C.acuminata might be involved in CPT synthesis regulation,which exhibit relatively high expression levels in the upper stem or immature bark.Among these,four genes(Cac AP2/ERF123,Cac AP2/ERF125,Cac AP2/ERF126,and Cac AP2/ERF127)belong to the ERF–B2 subgroup;two genes(Cac AP2/ERF149 and Cac AP2/ERF152)belong to the ERF–B3 subgroup;and two more genes(Cac AP2/ERF095 and Cac AP2/ERF096)belong to the DREB–A6 subgroup.These results provide a foundation for future functional characterization of the AP2/ERF genes to enhance the biosynthesis of CPT compounds of C.acuminata.展开更多
Two cardenolide glycosides,corotoxigenin 3-0-[β-D-glucopyranosyl-(l→4)-6-deoxy-β-D-glucopyranoside](1)and coroglaucigenin 3-0-[β-D-glucopyranosyl-(l→4)-6-deoxy-β-D-glucopyranoside](2),were isolated from the seed...Two cardenolide glycosides,corotoxigenin 3-0-[β-D-glucopyranosyl-(l→4)-6-deoxy-β-D-glucopyranoside](1)and coroglaucigenin 3-0-[β-D-glucopyranosyl-(l→4)-6-deoxy-β-D-glucopyranoside](2),were isolated from the seed fairs of Asclepias curassavica.The structures of 1-2 were determined based on the combination of the analysis of their MS,NMR spectroscopic data and acid hydrolysis.The inhibitory effects of compounds 1 and 2 on human colorectal carcinoma cells(HCT116),non-small cell lung carcinoma cells(A549)and hepatic cancer cells(SMMC-7721)were evaluated.The results showed that both compounds 1 and 2 significantly inhibited the viability,proliferation,and migration of A549,HCT116 and SMMC-7721 cells,suggesting that compounds 1 and 2 can be applied in the treatment of lung,colon and liver cancers in clinical practice.This study may not only provide a scientific basis for clarifying the active ingredients in A.curassavica,but also help to understand its antitumor activity,which can promote the application of A.curassavica in clinical treatment of various cancers.展开更多
文摘LBD(lateral organ boundaries)转录因子在植物生长发育和次生代谢调控中起着重要作用。为了发掘大麻LBD基因的功能,该研究在基因组和转录组水平上利用生物信息学手段,对大麻LBD基因家族进行系统鉴定,并对其表达模式进行分析。结果表明,大麻LBD基因家族含有32个成员,可分成2大类,7亚族,ClassⅠ分为5个亚族分别是ClassⅠ_a至ClassⅠ_e,ClassⅡ分为2个亚族分别是ClassⅡ_a和ClassⅡ_b;理化性质分析显示,大麻LBD基因家族编码的氨基酸数目为172~356,等电点为4.92~9.43,相对分子质量为18 862.92~40 081.33,大部分成员定位于细胞核中;染色体定位显示32个成员不均一地分布在大麻的10条染色体上;LBD转录因子结构域、基因结构和Motifs相对保守,不同类成员特征相近;基因上游启动子区含有多种植物激素及环境因子相关的顺式作用元件,表明LBD基因的表达可能会受到激素和外界环境因素的诱导;大麻LBD基因在ZYS品种(低四氢大麻酚,高二氢大麻酚)茎、叶、花的表达模式不同,LBD基因家族成员主要在ZYS品种的花和茎中表达,而在叶中表达的成员很少;ClassⅡ成员CsLBD21和CsLBD23在花和茎中表达,CsLBD8和CsLBD18在花、茎和叶中均有表达,这些基因可能参与大麻的生长发育发育进而影响大麻素的生物合成。该研究为后续大麻LBD基因家族的功能研究奠定了基础。
文摘目的Dof(DNA binding with one finger)基因家族是植物特有转录因子基因家族之一,在植物生长发育和其他非生物胁迫响应中起重要调控作用。本研究以工业大麻基因组中的Dof转录因子作为研究对象,针对Dof基因家族的基因结构、染色体定位、编码蛋白的理化性质及系统发育等方面进行分析。方法本研究通过生物信息学手段对工业大麻Dof基因进行鉴定和分析,使用PlantTFDB、ExPASy、MEME、WoLFPSORT、PlantCare等在线网站及TBTools、MEGA、Clustal、Gendoc等软件进行预测、可视化分析。结果研究发现,大麻中含有27个Dof基因成员,命名为CsDof1~CsDof27;染色体定位分析结果表明,27个Dof基因不均匀地分布在9条染色体上。由理化性质分析可知,氨基酸数目在185~512 aa之间,等电点介于4.91~9.22之间,所有Dof基因定位在细胞核中;通过对大麻和拟南芥Dof蛋白的系统发育分析,Dof基因家族可以被分为9个亚组。结论大麻Dof基因具有多个顺式作用元件,转录组数据表达热图显示部分大麻Dof基因具有茎和花组织表达特异性,未来研究将重点放在大麻花序中特异性表达的Dof基因上的功能研究。
基金supported by the National Key R&D Program of China(No.2019YFC1711100)the CAMS Innovation Fund for Medical Sciences(CIFMS,No.2016-I2M-3-016)。
文摘Camptotheca acuminata produces camptothecin(CPT),a monoterpene indole alkaloid(MIA)that is widely used in the treatment of lung,colorectal,cervical,and ovarian cancers.Its biosynthesis pathway has attracted significant attention,but the regulation of CPT biosynthesis by the APETALA2/ethylene-responsive factor(AP2/ERF)transcription factors(TFs)remains unclear.In this study,a systematic analysis of the AP2/ERF TFs family in C.acuminata was performed,including phylogeny,gene structure,conserved motifs,and gene expression profiles in different tissues and organs(immature bark,cotyledons,young flower,immature fruit,mature fruit,mature leaf,roots,upper stem,and lower stem)of C.acuminata.A total of 198 AP2/ERF genes were identified and divided into five relatively conserved subfamilies,including AP2(26 genes),DREB(61 genes),ERF(92 genes),RAV(18 genes),and Soloist(one gene).The combination of gene expression patterns in different C.acuminata tissues and organs,the phylogenetic tree,the co-expression analysis with biosynthetic genes,and the analysis of promoter sequences of key enzymes genes involved in CPT biosynthesis pathways revealed that eight AP2/ERF TFs in C.acuminata might be involved in CPT synthesis regulation,which exhibit relatively high expression levels in the upper stem or immature bark.Among these,four genes(Cac AP2/ERF123,Cac AP2/ERF125,Cac AP2/ERF126,and Cac AP2/ERF127)belong to the ERF–B2 subgroup;two genes(Cac AP2/ERF149 and Cac AP2/ERF152)belong to the ERF–B3 subgroup;and two more genes(Cac AP2/ERF095 and Cac AP2/ERF096)belong to the DREB–A6 subgroup.These results provide a foundation for future functional characterization of the AP2/ERF genes to enhance the biosynthesis of CPT compounds of C.acuminata.
基金supported by the Science and Technology Program of Guangzhou,China(No.201804010083)the National Natural Science Foundation of China(Nos.81720108033 and 81930114)+2 种基金Guangdong Key Laboratory for Translational Cancer Research of Chinese Medicine(No.2018B030322011)the Project of Guangzhou University of Chinese Medicine(No.QNYC20190103)the Project of Traditional Chinese Medicine Bureau of Guangdong Province(No.20211111).
文摘Two cardenolide glycosides,corotoxigenin 3-0-[β-D-glucopyranosyl-(l→4)-6-deoxy-β-D-glucopyranoside](1)and coroglaucigenin 3-0-[β-D-glucopyranosyl-(l→4)-6-deoxy-β-D-glucopyranoside](2),were isolated from the seed fairs of Asclepias curassavica.The structures of 1-2 were determined based on the combination of the analysis of their MS,NMR spectroscopic data and acid hydrolysis.The inhibitory effects of compounds 1 and 2 on human colorectal carcinoma cells(HCT116),non-small cell lung carcinoma cells(A549)and hepatic cancer cells(SMMC-7721)were evaluated.The results showed that both compounds 1 and 2 significantly inhibited the viability,proliferation,and migration of A549,HCT116 and SMMC-7721 cells,suggesting that compounds 1 and 2 can be applied in the treatment of lung,colon and liver cancers in clinical practice.This study may not only provide a scientific basis for clarifying the active ingredients in A.curassavica,but also help to understand its antitumor activity,which can promote the application of A.curassavica in clinical treatment of various cancers.
