The long non-coding RNA,Negative Regulator of Antiviral Response(NRAV)has been identified as a participant in both respiratory virus replication and immune checkpoints,however,its involvement in pan-cancer immune regu...The long non-coding RNA,Negative Regulator of Antiviral Response(NRAV)has been identified as a participant in both respiratory virus replication and immune checkpoints,however,its involvement in pan-cancer immune regulation and prognosis,particularly those of hepatocellular carcinoma(HCC),remains unclear.To address this knowledge gap,we analyzed expression profiles obtained from The Cancer Genome Atlas(TCGA)database,comparing normal and malignant tumor tissues.We found that NRAV expression is significantly upregulated in tumor tissues compared to adjacent nontumor tissues.Kaplan-Meier(K-M)analysis revealed the prognostic power of NRAV,wherein overexpression was significantly linked to reduced overall survival in a diverse range of tumor patients.Furthermore,noteworthy associations were observed between NRAV,immune checkpoints,immune cell infiltration,genes related to autophagy,epithelial-mesenchymal transition(EMT),pyroptosis,tumor mutational burden(TMB),and microsatellite instability(MSI)across different cancer types,including HCC.Moreover,NRAV upregulation expression was associated with multiple pathological stages by clinical observations.Furthermore,our investigation revealed a substantial elevation in the expression of NRAV in both HCC tumor tissues and cells compared to normal tissues and cells.The inhibition of NRAV resulted in the inhibition of cell proliferation,migration,and invasion in HCC cells,while also influencing the expression of CD274(PD-L1)and CD44,along with various biomarkers associated with EMT,autophagy,and pyroptosis.The aforementioned results propose NRAV as a promising prognostic biomarker for HCC.展开更多
In spring,summer,autumn 2015,we studied the seasonal variation of phytoplankton community structure in six rivers within Duobukuer Nature Reserve,and made preliminary evaluation of the water quality in Duobukuer Natur...In spring,summer,autumn 2015,we studied the seasonal variation of phytoplankton community structure in six rivers within Duobukuer Nature Reserve,and made preliminary evaluation of the water quality in Duobukuer Nature Reserve based on phytoplankton diversity.The results showed that a total of 7 phyla,41 genera,66 species and varieties of phytoplankton were identified in Duobukuer Nature Reserve;the annual species composition was dominated by diatom( 59%) which also preponderated in dominant species( 84. 3%),biomass( 68%)and abundance( 75%),with no significant seasonal variation. Shannon-Wiener diversity index( H') and Pielou evenness index( J) were used to evaluate the water quality in 13 sampling points,and the results showed that the water quality was good in Duobukuer Nature Reserve,the sampling points 5,6 and 12 presented β-pollution,and other sampling points were in an oligosaprobic-clean state.展开更多
基金funded by China National Natural Youth Science Foundation(81802078)Zhejiang Province Public Welfare Research Foundation(GF20H200021)Zhejiang Provincial Department of Medicine and Health Foundation(2019RC315).
文摘The long non-coding RNA,Negative Regulator of Antiviral Response(NRAV)has been identified as a participant in both respiratory virus replication and immune checkpoints,however,its involvement in pan-cancer immune regulation and prognosis,particularly those of hepatocellular carcinoma(HCC),remains unclear.To address this knowledge gap,we analyzed expression profiles obtained from The Cancer Genome Atlas(TCGA)database,comparing normal and malignant tumor tissues.We found that NRAV expression is significantly upregulated in tumor tissues compared to adjacent nontumor tissues.Kaplan-Meier(K-M)analysis revealed the prognostic power of NRAV,wherein overexpression was significantly linked to reduced overall survival in a diverse range of tumor patients.Furthermore,noteworthy associations were observed between NRAV,immune checkpoints,immune cell infiltration,genes related to autophagy,epithelial-mesenchymal transition(EMT),pyroptosis,tumor mutational burden(TMB),and microsatellite instability(MSI)across different cancer types,including HCC.Moreover,NRAV upregulation expression was associated with multiple pathological stages by clinical observations.Furthermore,our investigation revealed a substantial elevation in the expression of NRAV in both HCC tumor tissues and cells compared to normal tissues and cells.The inhibition of NRAV resulted in the inhibition of cell proliferation,migration,and invasion in HCC cells,while also influencing the expression of CD274(PD-L1)and CD44,along with various biomarkers associated with EMT,autophagy,and pyroptosis.The aforementioned results propose NRAV as a promising prognostic biomarker for HCC.
基金Supported by Project for Enhancing Effective Management of Protected Area Network in the Greater Khingan Mountains(UNDP PIMS:4824)National Natural Science Foundation of China(40830535)
文摘In spring,summer,autumn 2015,we studied the seasonal variation of phytoplankton community structure in six rivers within Duobukuer Nature Reserve,and made preliminary evaluation of the water quality in Duobukuer Nature Reserve based on phytoplankton diversity.The results showed that a total of 7 phyla,41 genera,66 species and varieties of phytoplankton were identified in Duobukuer Nature Reserve;the annual species composition was dominated by diatom( 59%) which also preponderated in dominant species( 84. 3%),biomass( 68%)and abundance( 75%),with no significant seasonal variation. Shannon-Wiener diversity index( H') and Pielou evenness index( J) were used to evaluate the water quality in 13 sampling points,and the results showed that the water quality was good in Duobukuer Nature Reserve,the sampling points 5,6 and 12 presented β-pollution,and other sampling points were in an oligosaprobic-clean state.
