Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blac...Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blackgram(V.mungo),and is distributed throughout the tropics.With its ability to tolerate salt stress,beach pea has great potential to contribute salt-tolerance genes for developing salt-tolerant cultivars in cultivated Vigna species.However,it is still underutilized in Vigna breeding programs.A draft genome sequence of beach pea was generated using a high-throughput next-generation sequencing platform,yielding 23.7 Gb of sequence from 79,929,868 filtered reads.A de novo genome assembly containing 68,731 scaffolds gave an N50 length of 10,272 bp and the assembled sequences totaled 365.6 Mb.A total of 35,448 SSRs,including 3574 compound SSRs,were identified and primer pairs for most of these SSRs were designed.Genome analysis identified 50,670 genes with mean coding sequence length 1042 bp.Phylogenetic analysis revealed highest sequence similarity with V.angularis,followed by V.radiata.Comparison with the V.angularis genome revealed 16,699 SNPs and 2253 InDels and comparison with the V.radiata genome revealed 17,538 SNPs and 2300 InDels.To our knowledge this is the first draft genome sequence of beach pea derived from an accession(ANBp-14-03)adapted locally in the Andaman and Nicobar Islands of India.The draft genome sequence may facilitate the genetic enhancement in cultivated Vigna species.展开更多
Grass pea(Lathyrus sativus L.) is a crop that is considered one of the more resilient to climate change. With protein-rich seeds and leaves, it has strong potential as human food as well as animal feed and fodder. How...Grass pea(Lathyrus sativus L.) is a crop that is considered one of the more resilient to climate change. With protein-rich seeds and leaves, it has strong potential as human food as well as animal feed and fodder. However, genetic improvement in this crop remains stagnant owing to the poor characterization of its genetic resources. In this study, we characterized 118 accessions of grass pea with 18 EST-SSR markers. A total of 118 accessions, 101 of L. sativus(100 cultivated accessions from Bangladesh and one wild accession) and 17 wild accessions of other Lathyrus species, were used. A total of 67 alleles were detected, with an average of 3.72 alleles per locus and average polymorphism information content of 0.52. A dissimilarity matrix was formed and hierarchical cluster analysis performed using the UPGMA method grouped genotypes into four main clusters. Cluster analysis based on the genetic dissimilarity revealed a clear grouping of the 100 cultivated and 18 wild accessions into four main groups. Group I consisted of 20 accessions with high β-N-oxalyl-L-α,β-diaminopropionic acid(β-ODAP) concentration. Of these 20 accessions, 17 were wild accessions. Only one wild accession(L. cicera) was clustered in group II, which contained 35 accessions. Most of the group II accessions contained low β-ODAP. Group III was represented by 34 accessions, many of them with high β-ODAP. Group IV consisted of 29 accessions, a few of which had very high β-ODAP concentrations. Analysis of molecular variance of the microsatellite data showed significantly higher values of molecular variance between(83%) than within(17%) populations. These characterized accessions will be useful in grass pea breeding programs.展开更多
Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain pro...Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain protein content (GPC) sug- gested that the major part of genetic variation for this trait is due to environmental interactions. In contrast, pre-harvest sprouting tolerance (PHST) was controlled mainly by main effect QTL (M-QTL) with very little genetic variation due to environmental interactions; a major QTL for PHST was detected on chromosome arm 3AL. For grain weight, one QTL each was detected on chromosome arms 1AS, 2BS and 7AS. QTL for 4 growth related traits taken together detected by different methods ranged from 37 to 40; nine QTL that were detected by single-locus as well as two-locus analyses were all M-QTL. Similarly, single-locus and two-locus QTL analyses for seven yield and yield contributing traits in two populations respectively allowed detection of 25 and 50 QTL by composite interval mapping (CIM), 16 and 25 QTL by multiple-trait composite interval mapping (MCIM) and 38 and 37 QTL by two-locus analyses. These studies should prove useful in QTL cloning and wheat improvement through marker aided selection.展开更多
The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropic...The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines (CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472, RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi, RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content (PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought.展开更多
Rohtak is one of the eight priority towns (Regional Centres) of National Capital Region (NCR) Regional Plan 2001. It lies 70 kms north-west from Delhi, the National Capital of India. Located at 28?41'1'' N...Rohtak is one of the eight priority towns (Regional Centres) of National Capital Region (NCR) Regional Plan 2001. It lies 70 kms north-west from Delhi, the National Capital of India. Located at 28?41'1'' North latitude and 76?12'42'' East longitude in the NCR region of Haryana state on National Highway No. 10, it spreads over 100.57 km2. Its population was projected to grow to 500,000 by 2001. Despite its nearness to the national capital it did not attract investment and the rate of urbanization remained low. Rohtak city grew slower than the state of Haryana in the three decades ending in 2001. On being declared as Municipal Corporation in 2010 urban growth of Rohtak took place in the form of extension in territorial jurisdiction and inclusion of urbanized eight villages. The new economic environment demands sustainable land management. Spatial information of land use/land cover types and their change detection in time series are important means for city planning and undertaking development activities. The present work is undertaken in that spirit. It has analyzed the relationship between urban growth and land use changes and their impact on Rohtak city. The period of focus is from 1983 to 2010. Both primary and secondary sources of data have been used for the present study. Secondary sources of data—guide map, topographical sheet and high resolution satellite imageries have been used to detect land use/land cover changes from the study area whereas primary source of data include ground truth and photographs from the field.展开更多
Ultrasonic attenuation studies can be used to characterize material not only after production but during processing as well. The most important causes of ultrasonic attenuation in solids are electron-phonon, phonon-ph...Ultrasonic attenuation studies can be used to characterize material not only after production but during processing as well. The most important causes of ultrasonic attenuation in solids are electron-phonon, phonon-phonon interaction and that due to thermo elastic relaxation. The two dominant processes that will give rise to appreciable ultrasonic attenuation at higher temperature are the phonon-phonon interaction also known as Akhiezer loss and that due to thermo elastic relaxation are observed in calcium oxide crystal. At frequencies of ultrasonic range and at higher temperatures in solids, phonon-phonon interaction mechanism is dominating cause for attenuation. Ultrasonic attenuation due to phonon-phonon interaction (α/f2)p-p and thermo elastic relaxation (α/f2)th are evaluated in Calcium Oxide crystal up to an elevated temperature from 100 K - 1500 K along , and crystallographic directions. Temperature dependence of ultrasonic attenuation along different crystallographic direction reveals some typical characteristic features.展开更多
The mixing properties of CuPd alloy in molten state at 1350 K have been studied by assuming strong interaction between Cu and Pd atoms. Regular associated solution model has been used for the study. The asymmetry in i...The mixing properties of CuPd alloy in molten state at 1350 K have been studied by assuming strong interaction between Cu and Pd atoms. Regular associated solution model has been used for the study. The asymmetry in integral excess free energy of mixing, heat of mixing and entropy of mixing has been well explained.展开更多
We have determined integral excess free energy of mixing, heat of mixing and entropy of mixing of NaPb alloys in molten state at 700 K. The observed asymmetry in the properties of mixing of NaPb alloy in molten state ...We have determined integral excess free energy of mixing, heat of mixing and entropy of mixing of NaPb alloys in molten state at 700 K. The observed asymmetry in the properties of mixing of NaPb alloy in molten state is successfully explained on the basis of regular associated solution model. The theoretical analysis reveals that the pairwise interaction energies between the species depend considerably on temperature.展开更多
Ganga is the most prestigious river of India.The COVID-19 lockdown may have forced us to stay indoors,but it has been boon for pollution-ridden Ganga and Yamuna.Plankton is tiny organisms drifting with water current,i...Ganga is the most prestigious river of India.The COVID-19 lockdown may have forced us to stay indoors,but it has been boon for pollution-ridden Ganga and Yamuna.Plankton is tiny organisms drifting with water current,influenced by river physical and chemical factors.During lockdown anthropogenic factors were reduced which affected water and plankton quality.Plankton samples were collected from the upstream of the river Ganga(Shankerghat,latitude 25030’28”N and longitude,81052’10”E)and Yamuna(near boat club,latitude 25024’29”N and longitude 81054’50”E)at Prayagraj,during national lockdown.In the before lockdown period(2019),total 28 planktonic taxa were recorded from the river Ganga,among them 10 taxa from Bacillariophyceae,15 from Chlorophyceae and 3 from Myxophyceae.While during LD period total 54 genera with 86 species was recorded(Bacillariophyceae 10 taxa,Chlorophyceae 23 taxa,Myxophyceae 9 taxa,Euglenophyceae 2 taxa,Dianophyceae,1,Rotiferea 7 taxa,Protozoa 2 taxa).Various species of green algae were observed in this small period of lockdown,some species were not observed since a long,like Pediastrum tetras,Scenedesmus abundans,Ankistrodesmus fusiformis,and Brachionus angularis.Various species of phytoplankton and zooplankton were in reproductive phase because river was flowing silently,without any internal and external disturbance.Ganga was more affected by anthropogenic activity and factory discharge than Yamuna So lack of chemicals in the water and minimum human interference favoured auto rejuvenation of Ganga in terms of plankton quality,diversity and reproduction behaviour.Such type of environmental changes may stimulate for origin of new species and disappear or reappear of various aquatic species.展开更多
In this work,we have explored wormhole(WH)solutions in F(R,L_(m))gravity by assuming the Morris-Thorne WH metric and F(R,Lm)=R/2+(1+γR)L_(m),whereγis the free model parameter.We determined the WH solutions by utiliz...In this work,we have explored wormhole(WH)solutions in F(R,L_(m))gravity by assuming the Morris-Thorne WH metric and F(R,Lm)=R/2+(1+γR)L_(m),whereγis the free model parameter.We determined the WH solutions by utilizing two newly developed shape functions(SF)that satisfy all basic conditions for a WH’s physical validity.We also observe that the null energy condition(NEC)behaves negatively.Finally,for both models,we use the volume integral quantifier(VIQ)and Tolman-Oppenheimer-Volkoff(TOV)equation to determine how much exotic matter is needed near the WH throat and the stability of the WH.The extensive detailed discussions of the matter components have been done via graphical analysis.The obtained WH geometries meet the physically acceptable conditions for a stable wormhole.展开更多
Previous work （Hussain et al. （2013）. Chemical Engineering Science, 101, 35） has pointed out that the conventional, one-dimensional population balance equation for aggregation can be expanded to accurately reprodu...Previous work （Hussain et al. （2013）. Chemical Engineering Science, 101, 35） has pointed out that the conventional, one-dimensional population balance equation for aggregation can be expanded to accurately reproduce the results of discrete simulations of spray fluidized bed agglomeration. However, some parameters had to be imported from the discrete simulation （Monte-Carlo）. The present paper shows how the expanded population balance can be run without importing parameters from the Monte-Carlo simulation. The expanded population balance still reproduces the results of Monte-Carlo simulations accurately, taking into account key micro-scale phenomena （sessile droplet drying, efficiency of collisions）, but with much lower computational cost. Required input parameters are just the drying time of sessile droplets （calculated in advance）, and the prefactor of an equation that correlates particle collision frequency with fluidized bed expansion. In this way, the expanded population balance is, apart from autonomous, also （nearly） predictive. Its performance is demonstrated by comparisons with both Monte-Carlo results and experimental data for various operating conditions （binder mass flow rate, gas temperature）. Despite formally being a one-dimensional expression, the expanded population balance captures additional properties, such as the number of wet particles and the number of droplets in the system, which are even difficult to measure in exoeriments.展开更多
Hydrothermal liquefaction(HTL)and pyrolysis(Py)of Loktak lake biomass mixture of water hyacinth(WH)and para grass(PG)were carried out at 260-300℃ and 300-500℃ to compare the products yield and chemical characteristi...Hydrothermal liquefaction(HTL)and pyrolysis(Py)of Loktak lake biomass mixture of water hyacinth(WH)and para grass(PG)were carried out at 260-300℃ and 300-500℃ to compare the products yield and chemical characteristics of the products.In case of HTL,the maximum bio-oil yield was obtained 13.34 wt.%at 280℃ while for Py,the maximum bio-oil yield was observed 38.8 wt.%at 350℃.The obtained bio-oils and bio-chars were analyzed using GC-MS,FT-IR,NMR,TGA,TOC,and SEM.GC-MS analysis of the bio-oils were showed that the HTL bio-oil majorly contains of nitrogen containing compounds whereas the Py bio-oil contains majority of phenolic compounds.Other compounds like ketones,alcohols,acids were also observed in bio-oil.Higher intensity broad band at 3300-3500 cm^(−1) was observed in the Py bio-oil compared to HTL bio-oil.TGA and proximate analysis of bio-char revealed the higher devolatilization occured during the HTL compared to Py process.The surface morphology of the HTL bio-char was found to be rough and fragmented as compared to the pyrolysis bio-char,clearly showing the biomass macromolecules breakdown differently in HTL and Py process.展开更多
In this paper, a robust fractional order fuzzy P ＋ fuzzy I ＋ fuzzy D （FOFP ＋ FOFI ＋ FOFD） controller is presented for a nonlinear and uncertain 2-1ink planar rigid manipulator. It is a nonlinear fuzzy controller...In this paper, a robust fractional order fuzzy P ＋ fuzzy I ＋ fuzzy D （FOFP ＋ FOFI ＋ FOFD） controller is presented for a nonlinear and uncertain 2-1ink planar rigid manipulator. It is a nonlinear fuzzy controller with variable gains that makes it self- adjustable or adaptive in nature. The fractional order operators further make it more robust by providing additional degrees of freedom to the design engineer. The integer order counterpart, fuzzy P ＋ fuzzy I ＋ fuzzy D （FP ＋ FI ＋ FD） controller, for a comparative study, was realized by taking the integer value for the fractional order operators in FOFP ＋ FOFI ＋ FOFD controller. The performances of both the fuzzy controllers are evaluated for reference trajectory tracking and disturbance rejection with and without model uncertainty and measurement noise. Genetic algorithm was used to optimize the parameters of controller under study for minimum integral of absolute error. Simulation results demonstrated that FOFP ＋ FOFI ＋ FOFD controller show much better performance as compared to its counterpart FP ＋ FI ＋ FD controller in servo as well as the regulatory problem and in model uncertainty and noisy environment FOFP ＋ FOFI ＋ FOFD controller demonstrated more robust behavior as compared to the FP ＋ FI ＋ FD controller. For the developed controller bounded-input and bounded-output stability conditions are also developed using Small Gain Theorem.展开更多
Microbes are ubiquitously distributed in nature, and recent culture-independent studies have highlighted the significance of gut microbiota in human health and disease. Fecal DNA is the primary source for the majority...Microbes are ubiquitously distributed in nature, and recent culture-independent studies have highlighted the significance of gut microbiota in human health and disease. Fecal DNA is the primary source for the majority of human gut microbiome studies. However, further improvement is needed to obtain fecal metagenomic DNA with sufficient amount and good quality but low host genomic DNA contamination. In the current study, we demonstrate a quick, robust, unbiased, and cost-effective method for the isolation of high molecular weight （〉 23 kb） metagenomic DNA （260/280 ratio 〉 1.8） with a good yield （55.8 ± 3.8 ng/mg of feces）. We also confirm that there is very low human genomic DNA contamination （eubacterial： human genomic DNA marker genes = 227.9：1） in the human feces. The newly-developed method robustly performs for fresh as well as stored fecal samples as demonstrated by 16S rRNA gene sequencing using 454 FLX＋. Moreover, 16S rRNA gene analysis indicated that compared to other DNA extraction methods tested, the fecal metagenomic DNA isolated with current methodology retains species richness and does not show microbial diversity biases, which is further confirmed by qPCR with a known quantity of spike-in genomes. Overall, our data highlight a protocol with a balance between quality, amount, user-friendliness, and cost effectiveness for its suitability toward usage for cultureindependent analysis of the human gut microbiome, which provides a robust solution to overcome key issues associated with fecal metagenomic DNA isolation in human gut microbiome studies.展开更多
Transport of suspended sediment in open channel flow has an enormous impact on real life situations,viz.control and management of reservoir sedimentation,geomorphic evolution such as dunes,rivers,and coastlines etc.Tr...Transport of suspended sediment in open channel flow has an enormous impact on real life situations,viz.control and management of reservoir sedimentation,geomorphic evolution such as dunes,rivers,and coastlines etc.Transport entails advection and diffusion.Turbulent diffusion is governed by the concept of Fick’s law,which is based on the molecular diffusion theory,and the equation that represents the distribution of sediment concentration is the advection-diffusion equation.The study uses the existing governing equation which considers different phases for solid and fluid,and then couples the two phases.To deal with high-concentrated flow,sediment and turbulent diffusion coefficients are taken to be different from each other.The effect of hindered settling on sediment particles is incorporated in the governing equation,which makes the equation highly non-linear.This study derives an explicit closed-form analytical solution to the generalized one-dimensional diffusion equation representing the vertical sediment concentration distribution with an arbitrary turbulent diffusion coefficient profile.The solution is obtained by Homotopy Analysis Method,which does not rely on the small parameters present in the equation.Finally,the solution is validated by comparing it with the implicit solution and the numerical solution.A relevant set of laboratory data is selected to check the applicability of the model,and a close agreement shows the potential of the model in the context of application to high-concentrated sediment-laden open channel flow.展开更多
Aggregation is one of the many important processes in chemical and process engineering. Several researchers have attempted to understand this complex process in fluidized beds using the macro-model of population balan...Aggregation is one of the many important processes in chemical and process engineering. Several researchers have attempted to understand this complex process in fluidized beds using the macro-model of population balance equations （PBEs）. The aggregation kernel is an effective parameter in PBEs, and is defined as the product of the aggregation efficiency and collision frequency functions. Attempts to derive this kernel have taken different approaches, including theoretical, experimental, and empirical techniques. The present paper calculates the aggregation kernel using micro-model computer simulations, i.e., a discrete particle model. We simulate the micro-model without aggregation for various initial conditions, and observe that the collision frequency function is in good agreement with the shear kernel. We then simulate the micro-model with aggregation and calculate the aggregation efficiency rate.展开更多
文摘Beach pea or beach cowpea(Vigna marina(Burm.)Merr.)belongs to the family Fabaceae.It is a close relative of cultivated Vigna species such as adzuki bean(V.angularis),cowpea(V.unguiculata),mung bean(V.radiata),and blackgram(V.mungo),and is distributed throughout the tropics.With its ability to tolerate salt stress,beach pea has great potential to contribute salt-tolerance genes for developing salt-tolerant cultivars in cultivated Vigna species.However,it is still underutilized in Vigna breeding programs.A draft genome sequence of beach pea was generated using a high-throughput next-generation sequencing platform,yielding 23.7 Gb of sequence from 79,929,868 filtered reads.A de novo genome assembly containing 68,731 scaffolds gave an N50 length of 10,272 bp and the assembled sequences totaled 365.6 Mb.A total of 35,448 SSRs,including 3574 compound SSRs,were identified and primer pairs for most of these SSRs were designed.Genome analysis identified 50,670 genes with mean coding sequence length 1042 bp.Phylogenetic analysis revealed highest sequence similarity with V.angularis,followed by V.radiata.Comparison with the V.angularis genome revealed 16,699 SNPs and 2253 InDels and comparison with the V.radiata genome revealed 17,538 SNPs and 2300 InDels.To our knowledge this is the first draft genome sequence of beach pea derived from an accession(ANBp-14-03)adapted locally in the Andaman and Nicobar Islands of India.The draft genome sequence may facilitate the genetic enhancement in cultivated Vigna species.
基金supported partially by Bioversity VavilovFrankel Fellowship to Priyanka Gupta(Grant no.CONT/12/217/RF)supported by Grains Research and Development Corporation(GRDC),Australia
文摘Grass pea(Lathyrus sativus L.) is a crop that is considered one of the more resilient to climate change. With protein-rich seeds and leaves, it has strong potential as human food as well as animal feed and fodder. However, genetic improvement in this crop remains stagnant owing to the poor characterization of its genetic resources. In this study, we characterized 118 accessions of grass pea with 18 EST-SSR markers. A total of 118 accessions, 101 of L. sativus(100 cultivated accessions from Bangladesh and one wild accession) and 17 wild accessions of other Lathyrus species, were used. A total of 67 alleles were detected, with an average of 3.72 alleles per locus and average polymorphism information content of 0.52. A dissimilarity matrix was formed and hierarchical cluster analysis performed using the UPGMA method grouped genotypes into four main clusters. Cluster analysis based on the genetic dissimilarity revealed a clear grouping of the 100 cultivated and 18 wild accessions into four main groups. Group I consisted of 20 accessions with high β-N-oxalyl-L-α,β-diaminopropionic acid(β-ODAP) concentration. Of these 20 accessions, 17 were wild accessions. Only one wild accession(L. cicera) was clustered in group II, which contained 35 accessions. Most of the group II accessions contained low β-ODAP. Group III was represented by 34 accessions, many of them with high β-ODAP. Group IV consisted of 29 accessions, a few of which had very high β-ODAP concentrations. Analysis of molecular variance of the microsatellite data showed significantly higher values of molecular variance between(83%) than within(17%) populations. These characterized accessions will be useful in grass pea breeding programs.
基金Project supported by the National Agricultural Technology Projectof Indian Council of Agricultural Research, Department of Biotech-nology of Government of India, Council of Scientific and IndustrialResearch of India and Indian National Science Academy
文摘Quantitative trait loci (QTL) analysis was conducted in bread wheat for 14 important traits utilizing data from four different mapping populations involving different approaches of QTL analysis. Analysis for grain protein content (GPC) sug- gested that the major part of genetic variation for this trait is due to environmental interactions. In contrast, pre-harvest sprouting tolerance (PHST) was controlled mainly by main effect QTL (M-QTL) with very little genetic variation due to environmental interactions; a major QTL for PHST was detected on chromosome arm 3AL. For grain weight, one QTL each was detected on chromosome arms 1AS, 2BS and 7AS. QTL for 4 growth related traits taken together detected by different methods ranged from 37 to 40; nine QTL that were detected by single-locus as well as two-locus analyses were all M-QTL. Similarly, single-locus and two-locus QTL analyses for seven yield and yield contributing traits in two populations respectively allowed detection of 25 and 50 QTL by composite interval mapping (CIM), 16 and 25 QTL by multiple-trait composite interval mapping (MCIM) and 38 and 37 QTL by two-locus analyses. These studies should prove useful in QTL cloning and wheat improvement through marker aided selection.
基金financial support from Department of Biotechnology (DBT) Ministry of Science and TechnologyIndian Council of Agricultural Research (ICAR) and the DirectorICAR-National Rice Research Institute, India, for providing all lab and field facilities
文摘The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines (CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472, RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi, RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content (PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought.
文摘Rohtak is one of the eight priority towns (Regional Centres) of National Capital Region (NCR) Regional Plan 2001. It lies 70 kms north-west from Delhi, the National Capital of India. Located at 28?41'1'' North latitude and 76?12'42'' East longitude in the NCR region of Haryana state on National Highway No. 10, it spreads over 100.57 km2. Its population was projected to grow to 500,000 by 2001. Despite its nearness to the national capital it did not attract investment and the rate of urbanization remained low. Rohtak city grew slower than the state of Haryana in the three decades ending in 2001. On being declared as Municipal Corporation in 2010 urban growth of Rohtak took place in the form of extension in territorial jurisdiction and inclusion of urbanized eight villages. The new economic environment demands sustainable land management. Spatial information of land use/land cover types and their change detection in time series are important means for city planning and undertaking development activities. The present work is undertaken in that spirit. It has analyzed the relationship between urban growth and land use changes and their impact on Rohtak city. The period of focus is from 1983 to 2010. Both primary and secondary sources of data have been used for the present study. Secondary sources of data—guide map, topographical sheet and high resolution satellite imageries have been used to detect land use/land cover changes from the study area whereas primary source of data include ground truth and photographs from the field.
文摘Ultrasonic attenuation studies can be used to characterize material not only after production but during processing as well. The most important causes of ultrasonic attenuation in solids are electron-phonon, phonon-phonon interaction and that due to thermo elastic relaxation. The two dominant processes that will give rise to appreciable ultrasonic attenuation at higher temperature are the phonon-phonon interaction also known as Akhiezer loss and that due to thermo elastic relaxation are observed in calcium oxide crystal. At frequencies of ultrasonic range and at higher temperatures in solids, phonon-phonon interaction mechanism is dominating cause for attenuation. Ultrasonic attenuation due to phonon-phonon interaction (α/f2)p-p and thermo elastic relaxation (α/f2)th are evaluated in Calcium Oxide crystal up to an elevated temperature from 100 K - 1500 K along , and crystallographic directions. Temperature dependence of ultrasonic attenuation along different crystallographic direction reveals some typical characteristic features.
文摘The mixing properties of CuPd alloy in molten state at 1350 K have been studied by assuming strong interaction between Cu and Pd atoms. Regular associated solution model has been used for the study. The asymmetry in integral excess free energy of mixing, heat of mixing and entropy of mixing has been well explained.
文摘We have determined integral excess free energy of mixing, heat of mixing and entropy of mixing of NaPb alloys in molten state at 700 K. The observed asymmetry in the properties of mixing of NaPb alloy in molten state is successfully explained on the basis of regular associated solution model. The theoretical analysis reveals that the pairwise interaction energies between the species depend considerably on temperature.
文摘Ganga is the most prestigious river of India.The COVID-19 lockdown may have forced us to stay indoors,but it has been boon for pollution-ridden Ganga and Yamuna.Plankton is tiny organisms drifting with water current,influenced by river physical and chemical factors.During lockdown anthropogenic factors were reduced which affected water and plankton quality.Plankton samples were collected from the upstream of the river Ganga(Shankerghat,latitude 25030’28”N and longitude,81052’10”E)and Yamuna(near boat club,latitude 25024’29”N and longitude 81054’50”E)at Prayagraj,during national lockdown.In the before lockdown period(2019),total 28 planktonic taxa were recorded from the river Ganga,among them 10 taxa from Bacillariophyceae,15 from Chlorophyceae and 3 from Myxophyceae.While during LD period total 54 genera with 86 species was recorded(Bacillariophyceae 10 taxa,Chlorophyceae 23 taxa,Myxophyceae 9 taxa,Euglenophyceae 2 taxa,Dianophyceae,1,Rotiferea 7 taxa,Protozoa 2 taxa).Various species of green algae were observed in this small period of lockdown,some species were not observed since a long,like Pediastrum tetras,Scenedesmus abundans,Ankistrodesmus fusiformis,and Brachionus angularis.Various species of phytoplankton and zooplankton were in reproductive phase because river was flowing silently,without any internal and external disturbance.Ganga was more affected by anthropogenic activity and factory discharge than Yamuna So lack of chemicals in the water and minimum human interference favoured auto rejuvenation of Ganga in terms of plankton quality,diversity and reproduction behaviour.Such type of environmental changes may stimulate for origin of new species and disappear or reappear of various aquatic species.
基金S Chaudhary expresses his gratitude to the Central University of Haryana for providing a University Research Fellowship (URF) under the Reg. No. 222019the Department of Mathematics, Central University of Haryanathe University Grant Commission (UGC), New Delhi, India under the NTA Ref. No. 211610000030 for providing financial support
文摘In this work,we have explored wormhole(WH)solutions in F(R,L_(m))gravity by assuming the Morris-Thorne WH metric and F(R,Lm)=R/2+(1+γR)L_(m),whereγis the free model parameter.We determined the WH solutions by utilizing two newly developed shape functions(SF)that satisfy all basic conditions for a WH’s physical validity.We also observe that the null energy condition(NEC)behaves negatively.Finally,for both models,we use the volume integral quantifier(VIQ)and Tolman-Oppenheimer-Volkoff(TOV)equation to determine how much exotic matter is needed near the WH throat and the stability of the WH.The extensive detailed discussions of the matter components have been done via graphical analysis.The obtained WH geometries meet the physically acceptable conditions for a stable wormhole.
基金financial support provided by the German Science Foundation(DFG) within the framework of graduate school GRK-1554by the Alexander von Humboldt Foundation(research fellowship for Jitendra Kumar)
文摘Previous work （Hussain et al. （2013）. Chemical Engineering Science, 101, 35） has pointed out that the conventional, one-dimensional population balance equation for aggregation can be expanded to accurately reproduce the results of discrete simulations of spray fluidized bed agglomeration. However, some parameters had to be imported from the discrete simulation （Monte-Carlo）. The present paper shows how the expanded population balance can be run without importing parameters from the Monte-Carlo simulation. The expanded population balance still reproduces the results of Monte-Carlo simulations accurately, taking into account key micro-scale phenomena （sessile droplet drying, efficiency of collisions）, but with much lower computational cost. Required input parameters are just the drying time of sessile droplets （calculated in advance）, and the prefactor of an equation that correlates particle collision frequency with fluidized bed expansion. In this way, the expanded population balance is, apart from autonomous, also （nearly） predictive. Its performance is demonstrated by comparisons with both Monte-Carlo results and experimental data for various operating conditions （binder mass flow rate, gas temperature）. Despite formally being a one-dimensional expression, the expanded population balance captures additional properties, such as the number of wet particles and the number of droplets in the system, which are even difficult to measure in exoeriments.
文摘Hydrothermal liquefaction(HTL)and pyrolysis(Py)of Loktak lake biomass mixture of water hyacinth(WH)and para grass(PG)were carried out at 260-300℃ and 300-500℃ to compare the products yield and chemical characteristics of the products.In case of HTL,the maximum bio-oil yield was obtained 13.34 wt.%at 280℃ while for Py,the maximum bio-oil yield was observed 38.8 wt.%at 350℃.The obtained bio-oils and bio-chars were analyzed using GC-MS,FT-IR,NMR,TGA,TOC,and SEM.GC-MS analysis of the bio-oils were showed that the HTL bio-oil majorly contains of nitrogen containing compounds whereas the Py bio-oil contains majority of phenolic compounds.Other compounds like ketones,alcohols,acids were also observed in bio-oil.Higher intensity broad band at 3300-3500 cm^(−1) was observed in the Py bio-oil compared to HTL bio-oil.TGA and proximate analysis of bio-char revealed the higher devolatilization occured during the HTL compared to Py process.The surface morphology of the HTL bio-char was found to be rough and fragmented as compared to the pyrolysis bio-char,clearly showing the biomass macromolecules breakdown differently in HTL and Py process.
文摘In this paper, a robust fractional order fuzzy P ＋ fuzzy I ＋ fuzzy D （FOFP ＋ FOFI ＋ FOFD） controller is presented for a nonlinear and uncertain 2-1ink planar rigid manipulator. It is a nonlinear fuzzy controller with variable gains that makes it self- adjustable or adaptive in nature. The fractional order operators further make it more robust by providing additional degrees of freedom to the design engineer. The integer order counterpart, fuzzy P ＋ fuzzy I ＋ fuzzy D （FP ＋ FI ＋ FD） controller, for a comparative study, was realized by taking the integer value for the fractional order operators in FOFP ＋ FOFI ＋ FOFD controller. The performances of both the fuzzy controllers are evaluated for reference trajectory tracking and disturbance rejection with and without model uncertainty and measurement noise. Genetic algorithm was used to optimize the parameters of controller under study for minimum integral of absolute error. Simulation results demonstrated that FOFP ＋ FOFI ＋ FOFD controller show much better performance as compared to its counterpart FP ＋ FI ＋ FD controller in servo as well as the regulatory problem and in model uncertainty and noisy environment FOFP ＋ FOFI ＋ FOFD controller demonstrated more robust behavior as compared to the FP ＋ FI ＋ FD controller. For the developed controller bounded-input and bounded-output stability conditions are also developed using Small Gain Theorem.
基金supported by the Council of Scientific and Industrial Research(CSIR)-sponsored research scheme[Grant No.60(0099)/11/EMRII]the Major Research Project of the University Grants Commission(UGC)(Grant No.411256/2012 SR),India+2 种基金supported by the fellowshipsthe Department of Biotechnology(DBT)the Department of Science and Technology(DST),India
文摘Microbes are ubiquitously distributed in nature, and recent culture-independent studies have highlighted the significance of gut microbiota in human health and disease. Fecal DNA is the primary source for the majority of human gut microbiome studies. However, further improvement is needed to obtain fecal metagenomic DNA with sufficient amount and good quality but low host genomic DNA contamination. In the current study, we demonstrate a quick, robust, unbiased, and cost-effective method for the isolation of high molecular weight （〉 23 kb） metagenomic DNA （260/280 ratio 〉 1.8） with a good yield （55.8 ± 3.8 ng/mg of feces）. We also confirm that there is very low human genomic DNA contamination （eubacterial： human genomic DNA marker genes = 227.9：1） in the human feces. The newly-developed method robustly performs for fresh as well as stored fecal samples as demonstrated by 16S rRNA gene sequencing using 454 FLX＋. Moreover, 16S rRNA gene analysis indicated that compared to other DNA extraction methods tested, the fecal metagenomic DNA isolated with current methodology retains species richness and does not show microbial diversity biases, which is further confirmed by qPCR with a known quantity of spike-in genomes. Overall, our data highlight a protocol with a balance between quality, amount, user-friendliness, and cost effectiveness for its suitability toward usage for cultureindependent analysis of the human gut microbiome, which provides a robust solution to overcome key issues associated with fecal metagenomic DNA isolation in human gut microbiome studies.
文摘Transport of suspended sediment in open channel flow has an enormous impact on real life situations,viz.control and management of reservoir sedimentation,geomorphic evolution such as dunes,rivers,and coastlines etc.Transport entails advection and diffusion.Turbulent diffusion is governed by the concept of Fick’s law,which is based on the molecular diffusion theory,and the equation that represents the distribution of sediment concentration is the advection-diffusion equation.The study uses the existing governing equation which considers different phases for solid and fluid,and then couples the two phases.To deal with high-concentrated flow,sediment and turbulent diffusion coefficients are taken to be different from each other.The effect of hindered settling on sediment particles is incorporated in the governing equation,which makes the equation highly non-linear.This study derives an explicit closed-form analytical solution to the generalized one-dimensional diffusion equation representing the vertical sediment concentration distribution with an arbitrary turbulent diffusion coefficient profile.The solution is obtained by Homotopy Analysis Method,which does not rely on the small parameters present in the equation.Finally,the solution is validated by comparing it with the implicit solution and the numerical solution.A relevant set of laboratory data is selected to check the applicability of the model,and a close agreement shows the potential of the model in the context of application to high-concentrated sediment-laden open channel flow.
基金supported by the Graduiertenkolleg-828,"Micro-Macro-Interactions in Structured Media and Particles Systems",Otto-von-Guericke-University Magdeburg
文摘Aggregation is one of the many important processes in chemical and process engineering. Several researchers have attempted to understand this complex process in fluidized beds using the macro-model of population balance equations （PBEs）. The aggregation kernel is an effective parameter in PBEs, and is defined as the product of the aggregation efficiency and collision frequency functions. Attempts to derive this kernel have taken different approaches, including theoretical, experimental, and empirical techniques. The present paper calculates the aggregation kernel using micro-model computer simulations, i.e., a discrete particle model. We simulate the micro-model without aggregation for various initial conditions, and observe that the collision frequency function is in good agreement with the shear kernel. We then simulate the micro-model with aggregation and calculate the aggregation efficiency rate.
