BACKGROUND Chromofungin(CHR:chromogranin-A 47-66)is a chromogranin-A derived peptide with anti-inflammatory and anti-microbial properties.Ulcerative colitis(UC)is characterized by a colonic decrease of CHR and a dysre...BACKGROUND Chromofungin(CHR:chromogranin-A 47-66)is a chromogranin-A derived peptide with anti-inflammatory and anti-microbial properties.Ulcerative colitis(UC)is characterized by a colonic decrease of CHR and a dysregulation of dendritic CD11c+cells.AIM To investigate the association between CHR treatment and dendritic cells(DCs)-related markers in different immune compartments in colitis.METHODS A model of acute UC-like colitis using dextran sulphate sodium(DSS)was used in addition to biopsies collected from UC patients.RESULTS Intrarectal CHR treatment reduced the severity of DSS-induced colitis and was associated with a significant decrease in the expression of CD11c,CD40,CD80,CD86 and interleukin(IL)-12p40 in the inflamed colonic mucosa and CD11c,CD80,CD86 IL-6 and IL-12p40 within the mesenteric lymph nodes and the spleen.Furthermore,CHR treatment decreased CD80 and CD86 expression markers of splenic CD11c+cells and decreased NF-κB expression in the colon and of splenic CD11c+cells.In vitro,CHR decreased CD40,CD80,CD86 IL-6 and IL-12p40 expression in naïve bone marrow-derived CD11c+DCs stimulated with lipopolysaccharide.Pharmacological studies demonstrated an impact of CHR on the NF-κB pathway.In patients with active UC,CHR level was reduced and showed a negative linear relationship with CD11c and CD86.CONCLUSION CHR has protective properties against intestinal inflammation via the regulation of DC-related markers and CD11c+cells.CHR could be a potential therapy of UC.展开更多
This cross-sectional study recruited consecutively 347 patients (178 males,169 females) aged 19 to 30 years with a diagnosis of MDD according to Diagnostic and Statistical Manual of Mental Disorders,Fifth Edition (DSM...This cross-sectional study recruited consecutively 347 patients (178 males,169 females) aged 19 to 30 years with a diagnosis of MDD according to Diagnostic and Statistical Manual of Mental Disorders,Fifth Edition (DSM-V) criteria who underwent in-person interviews,the mini international neuropsychiatric interview,and physical examinations by experienced psychiatrists at Division of Neuropsychiatry,Department of Neurology,Xuanwu Hospital of Capital Medical University (Beijing,China) between August 20,2015,and December 28,2018.All patients were excluded with anemia,thyroid dysfunction,mental retardation,neurologic conditions,bipolar disorders,schizophrenia,and other mental disorders.Depressive symptoms were assessed with the Hamilton rating scale for depression (HAMD-17) by three experienced psychological testing technicians who were blinded to all subjects' birth delivery types at the Neuropsychological Center of Department of Neurology of Xuanwu Hospital.The study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of Xuanwu Hospital.As a retrospective study and data analysis were performed anonymously,this study was exempt from the informed consent from patients.展开更多
基金Crohn's and Colitis Canada,No.P46601Canadian Foundation for Innovation,No.P39902+2 种基金Children's Hospital Research Institute of Manitoba,No.P45040Natural Sciences and Engineering Research Council,No.P41224and Manitoba Health Research Council,No.P41118(to Ghia JE).
文摘BACKGROUND Chromofungin(CHR:chromogranin-A 47-66)is a chromogranin-A derived peptide with anti-inflammatory and anti-microbial properties.Ulcerative colitis(UC)is characterized by a colonic decrease of CHR and a dysregulation of dendritic CD11c+cells.AIM To investigate the association between CHR treatment and dendritic cells(DCs)-related markers in different immune compartments in colitis.METHODS A model of acute UC-like colitis using dextran sulphate sodium(DSS)was used in addition to biopsies collected from UC patients.RESULTS Intrarectal CHR treatment reduced the severity of DSS-induced colitis and was associated with a significant decrease in the expression of CD11c,CD40,CD80,CD86 and interleukin(IL)-12p40 in the inflamed colonic mucosa and CD11c,CD80,CD86 IL-6 and IL-12p40 within the mesenteric lymph nodes and the spleen.Furthermore,CHR treatment decreased CD80 and CD86 expression markers of splenic CD11c+cells and decreased NF-κB expression in the colon and of splenic CD11c+cells.In vitro,CHR decreased CD40,CD80,CD86 IL-6 and IL-12p40 expression in naïve bone marrow-derived CD11c+DCs stimulated with lipopolysaccharide.Pharmacological studies demonstrated an impact of CHR on the NF-κB pathway.In patients with active UC,CHR level was reduced and showed a negative linear relationship with CD11c and CD86.CONCLUSION CHR has protective properties against intestinal inflammation via the regulation of DC-related markers and CD11c+cells.CHR could be a potential therapy of UC.
基金grants from the Beijing Muni cipal Science and Technology Project (No. Z171100000117016)National Natural Science Foundation of China (No. 81771862)+1 种基金the Beijing Natural Science Foundation (No. KZ201710025017), the Beijing Municipal Hospital Research and Development Plan (No. PX2017069)the Beijing Hundred, Thousand, and Ten Thousand Talents Project (No. 2017-CXYF-09).
文摘This cross-sectional study recruited consecutively 347 patients (178 males,169 females) aged 19 to 30 years with a diagnosis of MDD according to Diagnostic and Statistical Manual of Mental Disorders,Fifth Edition (DSM-V) criteria who underwent in-person interviews,the mini international neuropsychiatric interview,and physical examinations by experienced psychiatrists at Division of Neuropsychiatry,Department of Neurology,Xuanwu Hospital of Capital Medical University (Beijing,China) between August 20,2015,and December 28,2018.All patients were excluded with anemia,thyroid dysfunction,mental retardation,neurologic conditions,bipolar disorders,schizophrenia,and other mental disorders.Depressive symptoms were assessed with the Hamilton rating scale for depression (HAMD-17) by three experienced psychological testing technicians who were blinded to all subjects' birth delivery types at the Neuropsychological Center of Department of Neurology of Xuanwu Hospital.The study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of Xuanwu Hospital.As a retrospective study and data analysis were performed anonymously,this study was exempt from the informed consent from patients.
