Accurate and sensitive near-infrared(NIR)luminescent lateralflow immunoassay(LFIA)has attracted considerable attention in thefield of point-of-care testing(POCT).However,the detection accuracy and sensitivity are often ...Accurate and sensitive near-infrared(NIR)luminescent lateralflow immunoassay(LFIA)has attracted considerable attention in thefield of point-of-care testing(POCT).However,the detection accuracy and sensitivity are often compromised by the lowfluorescence quantum efficiency of the NIRfluorescent probe.(<10%)Herein,ultrabright NIR AIEgen nanoparticles(PS@AIE830NPs)composed of polystyrene(PS)nanoparticles and NIR aggregation-induced emission luminogen(AIEgen)with the maximum emission at 830 nm(AIE830)is reported,and its poten-tial to promote an accurate and sensitive detection of complex samples by LFIA is described.The relative quantum yield(QY)of the PS@AIE830NPs was 14.76%,which was superior to that of the polymer embedding method and indocyanine green(ICG)-based NIR nanoparticles.The PS@AIE830NPs immunolabeled-LFIA com-bined with laboratory-built NIR-LFIA portable quantitative instruments(detected light range 800 nm)completely eliminated background interference and allowed>highly accurate and sensitive detection without any pre-treatment steps.The limits of detection(LODs)for aflatoxin B1(AFB1)in soy sauce,alpha hemolysin(Hla)of Staphylococcus aureus biomarker in jointfluid,and C-reactive protein(CRP)in human haemolysed samples were 0.01 ng mL-1,0.02µg mL-1,and 0.156 mg L-1,respectively,commensurating with those of the corresponding gold standard assays and covering the detection range of interests.It is anticipated that the ultrabright NIR AIEgen nanoparticles will serve as a universally applicable signal probe for NIR-LFIA diagnostics,promising to expand the range of applications for quantitative detection of complex samples.展开更多
基金National Natural Science Foundation of China,Grant/Award Numbers:82202642,82302646,32172296Chongqing Postdoctoral Innovation Talent Support Program,Grant/Award Number:CQBX202218+8 种基金China Postdoctoral Science Foundation,Grant/Award Numbers:2022M710558,2023T160771Special foundation of Chongqing Postdoctoral Research Programme,Grant/Award Number:2021XM2036Talent Training Foundation of Key Laboratory of Clinical Laboratory Diagnostics(Ministry of Education)of College of Laboratory Medicine,Grant/Award Number:JYPY202202Chongqing Postdoctoral Science Foundation,Grant/Award Number:CSTB2022NSCQ-BHX0689National Natural Science Foundation Key Program,Grant/Award Numbers:82230032,81930023Key Project of Chongqing Science and Technology Bureau,Grant/Award Number:CSTC2021jscx-gksb-N0010Chongqing Outstanding Scientists Project(2019)Chongqing Chief Medical Scientist Project(2018)Chongqing Science and Technology Bureau Mountaineering Project,Grant/Award Numbers:cyyy-xkdfjh-jcyj-202301,cyyy-xkdfjh-lcyj-202303,cyyy-xkdfjh-cgzh-202302。
文摘Accurate and sensitive near-infrared(NIR)luminescent lateralflow immunoassay(LFIA)has attracted considerable attention in thefield of point-of-care testing(POCT).However,the detection accuracy and sensitivity are often compromised by the lowfluorescence quantum efficiency of the NIRfluorescent probe.(<10%)Herein,ultrabright NIR AIEgen nanoparticles(PS@AIE830NPs)composed of polystyrene(PS)nanoparticles and NIR aggregation-induced emission luminogen(AIEgen)with the maximum emission at 830 nm(AIE830)is reported,and its poten-tial to promote an accurate and sensitive detection of complex samples by LFIA is described.The relative quantum yield(QY)of the PS@AIE830NPs was 14.76%,which was superior to that of the polymer embedding method and indocyanine green(ICG)-based NIR nanoparticles.The PS@AIE830NPs immunolabeled-LFIA com-bined with laboratory-built NIR-LFIA portable quantitative instruments(detected light range 800 nm)completely eliminated background interference and allowed>highly accurate and sensitive detection without any pre-treatment steps.The limits of detection(LODs)for aflatoxin B1(AFB1)in soy sauce,alpha hemolysin(Hla)of Staphylococcus aureus biomarker in jointfluid,and C-reactive protein(CRP)in human haemolysed samples were 0.01 ng mL-1,0.02µg mL-1,and 0.156 mg L-1,respectively,commensurating with those of the corresponding gold standard assays and covering the detection range of interests.It is anticipated that the ultrabright NIR AIEgen nanoparticles will serve as a universally applicable signal probe for NIR-LFIA diagnostics,promising to expand the range of applications for quantitative detection of complex samples.
