将松浦红镜鲤(Cyprinus carpio Songpu red mirror)、散鳞镜鲤(Cyprinus carpio L.mirror)和荷包红鲤(Cyprinus carpio red var.Vuguanensis)抗寒品系乌仔各705尾放养到0.047hm^2池塘中,同塘培育到鱼种;春片鱼种各500尾放养到0.2hm^2池...将松浦红镜鲤(Cyprinus carpio Songpu red mirror)、散鳞镜鲤(Cyprinus carpio L.mirror)和荷包红鲤(Cyprinus carpio red var.Vuguanensis)抗寒品系乌仔各705尾放养到0.047hm^2池塘中,同塘培育到鱼种;春片鱼种各500尾放养到0.2hm^2池塘中;各池塘的投喂和饲养管理相同,按GB/T18654.2-2008和GB/T18654.3-2008标准测定鱼体质量和形态性状。结果显示,松浦红镜鲤体长/体高、体长/尾柄高、体长/头长、头长/吻长大于散鳞镜鲤和荷包红鲤抗寒品系;三个品种的8个可量比例性状主成分分析构建的3个主成分贡献率分别为:50.94%、17.08%、13.68%,累计贡献率达81.70%;主成分散点图显示,松浦红镜鲤聚成独立一簇,与其他品种明显区分;特定增重率、相对增重率、净增重、饲养成活率及越冬成活率等主要经济性状均显著高于荷包红鲤抗寒品系(P<0.05),但与散鳞镜鲤无明显差异。展开更多
目的·评价项目研制的可用于轻度认知功能障碍筛查的电子化认知评估系统的信度和效度,构建机器学习法判定模型并评估筛查效果。方法·采用分层随机的方法在上海和河南农村的社区、老年护理院及专科门诊抽取55岁以上的符合标准...目的·评价项目研制的可用于轻度认知功能障碍筛查的电子化认知评估系统的信度和效度,构建机器学习法判定模型并评估筛查效果。方法·采用分层随机的方法在上海和河南农村的社区、老年护理院及专科门诊抽取55岁以上的符合标准的老年人,由经过严格培训、操作规范的调查员对研究对象进行蒙特利尔认知评估量表(Montreal Cognitive Assessment,MoCA)的现场测试。电子化认知评估系统信度评价采用内部一致性系数,效度评价采用因子分析;以MoCA评估结果作为标准,使用分类准确率和曲线下面积(area under curve,AUC)比较朴素贝叶斯、随机森林、Logistic回归和K-邻近4种机器学习算法的分类效果。结果·研究的359名对象中,年龄中位数为63岁,82.80%为中学及以下学历;根据MoCA评分,可能患有轻度认知功能障碍的有147名。电子化认知评估系统的Cronbach'sα为0.84,KMO为0.78,Bartlett's球形检验P<0.05,共提取13个公因子,累计方差贡献率为75.10%。最优朴素贝叶斯分类模型的分类准确率为88.05%,AUC为0.941。结论·该电子化认知评估系统具有良好的信度、效度及分类效果,利用朴素贝叶斯分类模型分类准确度较高。展开更多
Chinese mitten crab(Eriocheir sinensis) is an indigenous and ecologically and economically important species in the Liao River area, but its identification and genetic diversity remain poorly understood. To evaluate t...Chinese mitten crab(Eriocheir sinensis) is an indigenous and ecologically and economically important species in the Liao River area, but its identification and genetic diversity remain poorly understood. To evaluate the germplasm resources of this species, samples were collected from these locations: four sub-populations from the Liao River area and one population from the Yangtze River area; one primer was used to distinguish between the Liao River and the Yangtze River crabs. Thirteen loci were used for crab genetic diversity analysis, and basic statistics showed that the collecting samples were purebred in the Liao River area. The average observed heterozygosity(H_0) of the Liao River population was 0.5931, and the expected heterozygosity(H_e) was 0.8064. The polymorphism information content(PIC) was 0.7753, which showed that the Liao River population had high genetic diversity. The genetic differentiation index(F_(ST)) averaged 0.0342, meaning a low degree of differentiation; cluster analysis indicated that Hujia(HJ), Xinli(XL) and Chenjia(CJ) sub-populations were allocated to the same cluster, while Baqiangzi(BQZ) sub-population was isolated. In summary, these data demonstrated that the crabs in the Liao River had high genetic diversity, but low genetic differentiation. Thus, the Liao River population had the potential for breeding selection. Furthermore, this study also provided valuable genetic information for the conservation of Chinese mitten crab.展开更多
There is a debate on if aquaculture tank colure could effect survival and growth of fish juveniles,it seems that different fish shows difference in the same tank colure environment or different stage shows difference ...There is a debate on if aquaculture tank colure could effect survival and growth of fish juveniles,it seems that different fish shows difference in the same tank colure environment or different stage shows difference in the same tank colure. The overall aim of this study is to establish whether tank colure influence survival and growth of Oncorhynchus masou juvenile under artificial controlled conditions. After an 50- days experiment,no significant difference of dead rate was observed in both blue and black tanks,and black tank led to significantly lower final body weight, fork length,as well as SGR. The study shows that rearing in blue tank should be considered little stressful for O. masou masou juveniles and blue material tank should be used in aquaculture practice.展开更多
文摘将松浦红镜鲤(Cyprinus carpio Songpu red mirror)、散鳞镜鲤(Cyprinus carpio L.mirror)和荷包红鲤(Cyprinus carpio red var.Vuguanensis)抗寒品系乌仔各705尾放养到0.047hm^2池塘中,同塘培育到鱼种;春片鱼种各500尾放养到0.2hm^2池塘中;各池塘的投喂和饲养管理相同,按GB/T18654.2-2008和GB/T18654.3-2008标准测定鱼体质量和形态性状。结果显示,松浦红镜鲤体长/体高、体长/尾柄高、体长/头长、头长/吻长大于散鳞镜鲤和荷包红鲤抗寒品系;三个品种的8个可量比例性状主成分分析构建的3个主成分贡献率分别为:50.94%、17.08%、13.68%,累计贡献率达81.70%;主成分散点图显示,松浦红镜鲤聚成独立一簇,与其他品种明显区分;特定增重率、相对增重率、净增重、饲养成活率及越冬成活率等主要经济性状均显著高于荷包红鲤抗寒品系(P<0.05),但与散鳞镜鲤无明显差异。
文摘目的·评价项目研制的可用于轻度认知功能障碍筛查的电子化认知评估系统的信度和效度,构建机器学习法判定模型并评估筛查效果。方法·采用分层随机的方法在上海和河南农村的社区、老年护理院及专科门诊抽取55岁以上的符合标准的老年人,由经过严格培训、操作规范的调查员对研究对象进行蒙特利尔认知评估量表(Montreal Cognitive Assessment,MoCA)的现场测试。电子化认知评估系统信度评价采用内部一致性系数,效度评价采用因子分析;以MoCA评估结果作为标准,使用分类准确率和曲线下面积(area under curve,AUC)比较朴素贝叶斯、随机森林、Logistic回归和K-邻近4种机器学习算法的分类效果。结果·研究的359名对象中,年龄中位数为63岁,82.80%为中学及以下学历;根据MoCA评分,可能患有轻度认知功能障碍的有147名。电子化认知评估系统的Cronbach'sα为0.84,KMO为0.78,Bartlett's球形检验P<0.05,共提取13个公因子,累计方差贡献率为75.10%。最优朴素贝叶斯分类模型的分类准确率为88.05%,AUC为0.941。结论·该电子化认知评估系统具有良好的信度、效度及分类效果,利用朴素贝叶斯分类模型分类准确度较高。
基金Supported by Open Project Program of Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture,Heilongjiang Fisheries Research Institute,Chinese Academy of Fishery Sciences(FBB2016-01)
文摘Chinese mitten crab(Eriocheir sinensis) is an indigenous and ecologically and economically important species in the Liao River area, but its identification and genetic diversity remain poorly understood. To evaluate the germplasm resources of this species, samples were collected from these locations: four sub-populations from the Liao River area and one population from the Yangtze River area; one primer was used to distinguish between the Liao River and the Yangtze River crabs. Thirteen loci were used for crab genetic diversity analysis, and basic statistics showed that the collecting samples were purebred in the Liao River area. The average observed heterozygosity(H_0) of the Liao River population was 0.5931, and the expected heterozygosity(H_e) was 0.8064. The polymorphism information content(PIC) was 0.7753, which showed that the Liao River population had high genetic diversity. The genetic differentiation index(F_(ST)) averaged 0.0342, meaning a low degree of differentiation; cluster analysis indicated that Hujia(HJ), Xinli(XL) and Chenjia(CJ) sub-populations were allocated to the same cluster, while Baqiangzi(BQZ) sub-population was isolated. In summary, these data demonstrated that the crabs in the Liao River had high genetic diversity, but low genetic differentiation. Thus, the Liao River population had the potential for breeding selection. Furthermore, this study also provided valuable genetic information for the conservation of Chinese mitten crab.
基金financially supported by Central-level Non-profit Scientific Research Institutes Special Funds(Project no.HSY201305)
文摘There is a debate on if aquaculture tank colure could effect survival and growth of fish juveniles,it seems that different fish shows difference in the same tank colure environment or different stage shows difference in the same tank colure. The overall aim of this study is to establish whether tank colure influence survival and growth of Oncorhynchus masou juvenile under artificial controlled conditions. After an 50- days experiment,no significant difference of dead rate was observed in both blue and black tanks,and black tank led to significantly lower final body weight, fork length,as well as SGR. The study shows that rearing in blue tank should be considered little stressful for O. masou masou juveniles and blue material tank should be used in aquaculture practice.
