A micro-nano structure CaF_(2)chemical conversion layer was prepared on fluoride-treated AZ31 alloy,then the composite fluoride conversion film(CaF_(2)/MgF_(2))was modified by stearic acid(SA)and fabricated a superhyd...A micro-nano structure CaF_(2)chemical conversion layer was prepared on fluoride-treated AZ31 alloy,then the composite fluoride conversion film(CaF_(2)/MgF_(2))was modified by stearic acid(SA)and fabricated a superhydrophobic surface.The fluoride-treated magnesium,fluoride conversion film and superhydrophobic coating were characterized by SEM,EDS,XRD and FTIR.The properties of coatings1 adhesion and corrosion resistance were evaluated via tape test and electrochemical measurement.The cytocompatibility of the MgF_(2),CaF_(2)and superhydrophobic CaF_(2)/SA surface was investigated with bone marrow-derived mesenchymal stem cells(BMSCs)by direct culture for 24 h.The results showed that the superhydrophobic fluoride conversion coating composed of inner MgF_(2)layer and the outer CaF_(2)/SA composite layer had an average water contact angle of 152°.SA infiltrated into the micro-nano structure CaF_(2)layer and formed a strong adhesion with CaF_(2)layer.Furthermore,the super-hydrophobic coating showed higher barrier properties and corrosion resistance compared with the fluoride conversion film and fluoride-treated AZ31 alloy.The BMSC adhesion test results demonstrated MgF_(2)CaF_(2)and CaF_(2)/SA coatings were all nontoxic to BMSC.At the condition of in direct contact with cells,MgF_(2)showed higher cell density and enhanced the BMSCs proliferation,while CaF_(2)and CaF_(2)/SA coating showed no statistically difference in cell density compared with glass reference but the CaF_(2)and CaF_(2)/SA coating were not conducive to BMSCs adhesion.展开更多
Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of terpenes.However,other functions of elaioplasts have not been fully characterized to date.Here,a LC–MS/MS shotgun technology w...Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of terpenes.However,other functions of elaioplasts have not been fully characterized to date.Here,a LC–MS/MS shotgun technology was applied to identify the proteins from elaioplasts that were highly purified from young fruit peel of kumquat.A total of 655 putative plastid proteins were identified from elaioplasts according to sequence homology in silico and manual curation.Based on functional classification via Mapman,~50%of the identified proteins fall into six categories,including protein metabolism,transport,and lipid metabolism.Of note,elaioplasts contained ATP synthase and ADP,ATP carrier proteins at high abundance,indicating important roles for ATP generation and transport in elaioplast biogenesis.Additionally,a comparison of proteins between citrus chromoplast and elaioplast proteomes suggest a high level of functional conservation.However,some distinctive protein profiles were also observed in both types of plastids notably for isoprene biosynthesis in elaioplasts,and carotenoid metabolism in chromoplasts.In conclusion,this comprehensive proteomic study provides new insights into the major metabolic pathways and unique characteristics of elaioplasts and chromoplasts in citrus fruit.展开更多
Duchenne muscular dystrophy(DMD)is a severe neuromuscular disorder caused by mutations in the dystrophin gene,which encodes the essential protein dystrophin.This genetic condition,affecting approximately 1 in 5000 mal...Duchenne muscular dystrophy(DMD)is a severe neuromuscular disorder caused by mutations in the dystrophin gene,which encodes the essential protein dystrophin.This genetic condition,affecting approximately 1 in 5000 male births worldwide(Birnkrant et al.,2018),is characterized by progressive muscle weakness and wasting,leading to premature death in the second or third decade of life(Farini et al.,2016).The absence of dystrophin protein causes instability in the sarcolemma,which predisposes individuals to myonecrosis and activation of inflammatory signaling cascades.Unfortunately,there is currently no effective cure for DMD,and glucocorticoid steroids are commonly used in clinical settings to delay symptom development(Merlini et al.,2003).展开更多
A portable device was designed and constructed for studying the properties of biomaterials in physiologically relevant fluids under controllable flow conditions that closely simulate fluid flow inside the body.The dev...A portable device was designed and constructed for studying the properties of biomaterials in physiologically relevant fluids under controllable flow conditions that closely simulate fluid flow inside the body.The device can fit entirely inside a cell incubator;and,thus,it can be used directly under standard cell culture conditions.An impedance-driven pump was built in the sterile flow loop to control the flow rates of fluids,which made the device small and portable for easy deployment in the incubator.To demonstrate the device functions,magnesium(Mg)as a representative biodegradable material was tested in the flow device for immersion degradation under flow versus static conditions,while the flow module was placed inside a standard cell incubator.The flow rate was controlled at 0.1760.06 ml/s for this study;and,the flow rate is adjustable through the controller module outside of incubators for simulating the flow rates in the ranges of blood flow in human artery(0.050.43 ml/s)and vein(0.020.08 ml/s).Degradation of Mg under flow versus static conditions was characterized by measuring the changes of sample mass and thickness,and Mg2t ion concentrations in the immersion media.Surface chemistry and morphology of Mg after immersion under flow versus static conditions were compared.The portable impedance-driven flow device is easy to fit inside an incubator and much smaller than a peristaltic pump,providing a valuable solution for studying biomaterials and implants(e.g.vascular or ureteral stents)in body fluids under flow versus static conditions with or without cells.展开更多
基金supported by the National Natural Science Foundation of China[Grant No.51201192]Natural Science Foundation of Chongqing[Grant No.cstc2018jcyj A2285]。
文摘A micro-nano structure CaF_(2)chemical conversion layer was prepared on fluoride-treated AZ31 alloy,then the composite fluoride conversion film(CaF_(2)/MgF_(2))was modified by stearic acid(SA)and fabricated a superhydrophobic surface.The fluoride-treated magnesium,fluoride conversion film and superhydrophobic coating were characterized by SEM,EDS,XRD and FTIR.The properties of coatings1 adhesion and corrosion resistance were evaluated via tape test and electrochemical measurement.The cytocompatibility of the MgF_(2),CaF_(2)and superhydrophobic CaF_(2)/SA surface was investigated with bone marrow-derived mesenchymal stem cells(BMSCs)by direct culture for 24 h.The results showed that the superhydrophobic fluoride conversion coating composed of inner MgF_(2)layer and the outer CaF_(2)/SA composite layer had an average water contact angle of 152°.SA infiltrated into the micro-nano structure CaF_(2)layer and formed a strong adhesion with CaF_(2)layer.Furthermore,the super-hydrophobic coating showed higher barrier properties and corrosion resistance compared with the fluoride conversion film and fluoride-treated AZ31 alloy.The BMSC adhesion test results demonstrated MgF_(2)CaF_(2)and CaF_(2)/SA coatings were all nontoxic to BMSC.At the condition of in direct contact with cells,MgF_(2)showed higher cell density and enhanced the BMSCs proliferation,while CaF_(2)and CaF_(2)/SA coating showed no statistically difference in cell density compared with glass reference but the CaF_(2)and CaF_(2)/SA coating were not conducive to BMSCs adhesion.
基金This work was supported by the National Natural Science Foundation of China(NSFC,grant nos.31501739)the National Basic Research Program of China(973 project No.2013CB127105)Huazhong Agricultural University Scientific&Technological Self-innovation Foundation(No.2662015BQ034).
文摘Elaioplasts of citrus peel are colorless plastids which accumulate significant amounts of terpenes.However,other functions of elaioplasts have not been fully characterized to date.Here,a LC–MS/MS shotgun technology was applied to identify the proteins from elaioplasts that were highly purified from young fruit peel of kumquat.A total of 655 putative plastid proteins were identified from elaioplasts according to sequence homology in silico and manual curation.Based on functional classification via Mapman,~50%of the identified proteins fall into six categories,including protein metabolism,transport,and lipid metabolism.Of note,elaioplasts contained ATP synthase and ADP,ATP carrier proteins at high abundance,indicating important roles for ATP generation and transport in elaioplast biogenesis.Additionally,a comparison of proteins between citrus chromoplast and elaioplast proteomes suggest a high level of functional conservation.However,some distinctive protein profiles were also observed in both types of plastids notably for isoprene biosynthesis in elaioplasts,and carotenoid metabolism in chromoplasts.In conclusion,this comprehensive proteomic study provides new insights into the major metabolic pathways and unique characteristics of elaioplasts and chromoplasts in citrus fruit.
基金supported by grants from National Natural Science Foundation of China(82202052,82230039,U2005201,81870902,82001217,31925016,and 82021001)Major Scientific Research Project for Middle-age and Youth of Fujian Provincial Health Commission grant(2021ZQNZD003)+2 种基金Major Scientific Research Project of Fujian Provincial Health Commission grant(2022ZD01002)Joint Funds for the Innovation of Science and Technology of Fujian Province(2018Y9082)the Youth Scientific Research。
文摘Duchenne muscular dystrophy(DMD)is a severe neuromuscular disorder caused by mutations in the dystrophin gene,which encodes the essential protein dystrophin.This genetic condition,affecting approximately 1 in 5000 male births worldwide(Birnkrant et al.,2018),is characterized by progressive muscle weakness and wasting,leading to premature death in the second or third decade of life(Farini et al.,2016).The absence of dystrophin protein causes instability in the sarcolemma,which predisposes individuals to myonecrosis and activation of inflammatory signaling cascades.Unfortunately,there is currently no effective cure for DMD,and glucocorticoid steroids are commonly used in clinical settings to delay symptom development(Merlini et al.,2003).
基金American Heart Association(AHA 12SDG12220014)International Collaboration Program of Zhejiang Province(2015C34007)for financial support.
文摘A portable device was designed and constructed for studying the properties of biomaterials in physiologically relevant fluids under controllable flow conditions that closely simulate fluid flow inside the body.The device can fit entirely inside a cell incubator;and,thus,it can be used directly under standard cell culture conditions.An impedance-driven pump was built in the sterile flow loop to control the flow rates of fluids,which made the device small and portable for easy deployment in the incubator.To demonstrate the device functions,magnesium(Mg)as a representative biodegradable material was tested in the flow device for immersion degradation under flow versus static conditions,while the flow module was placed inside a standard cell incubator.The flow rate was controlled at 0.1760.06 ml/s for this study;and,the flow rate is adjustable through the controller module outside of incubators for simulating the flow rates in the ranges of blood flow in human artery(0.050.43 ml/s)and vein(0.020.08 ml/s).Degradation of Mg under flow versus static conditions was characterized by measuring the changes of sample mass and thickness,and Mg2t ion concentrations in the immersion media.Surface chemistry and morphology of Mg after immersion under flow versus static conditions were compared.The portable impedance-driven flow device is easy to fit inside an incubator and much smaller than a peristaltic pump,providing a valuable solution for studying biomaterials and implants(e.g.vascular or ureteral stents)in body fluids under flow versus static conditions with or without cells.
