Objective:As a medicinal plant,the resource of Rhodiola dumulosa is deficient along with the large collection.For the protection and utilization of R.dumulosa,the influence of plant growth regulators(PGRs)on callus in...Objective:As a medicinal plant,the resource of Rhodiola dumulosa is deficient along with the large collection.For the protection and utilization of R.dumulosa,the influence of plant growth regulators(PGRs)on callus induction and adventitious shoots differentiation,polysaccharide production and the antioxidant activity were tested.Methods:Internodes of R.dumulosa were used as explants and cultured on MS medium plus different plant growth regulators(PGRs).The anti-oxidative activities of polysaccharides were evaluated using radical scavenging assays.Results:By response surface plot,0.85 mg/L N6-benzyladenine(BA),0.34 mg/L naphthaleneacetic acid(NAA)and 0.33 mg/L 2,4-dicholorophenoxyacetic acid(2,4-D)were the optimal factors for callus induction(90.03%)from internodes explants on MS medium.The fresh weight of green callus increased 47.26 fold,when callus was inoculated on MS+thidiazuron(TDZ)0.5 mg/L+NAA 2.0 mg/L.Adventitious buds regenerated from callus on the media of MS were fortified with BA 1.0 mg/L plus NAA 0.5 mg/L,and the induction rate was 40.00%.MS plus indole-3-butyric acid(IBA)1.0 mg/L produced the highest rooting rate with 10 to 15 roots in a length of 2–3 cm per shoot.The content of total polysaccharides in callus developed on MS+TDZ 0.5 mg/L+NAA 2.0 mg/L and MS+BA 1.0 mg/L+NAA 0.5 mg/L was as high as 1.72%2.15%.At the dose of 0.5 mg/mL polysaccharides extracted from different callus induced on MS+NAA 2.0 mg/L+TDZ 0.5 mg/L or MS+BA 1.0 mg/L+NAA 0.5 mg/L or MS+BA 0.5 mg/L+2,4-D 0.5 mg/L,the ABTS radical eliminating percentages were 82.78%,80.18%and 68.59%,respectively,much higher than that of wild plant.Conclusion:A rapid micropropagation system for R.dumulosa has been developed.The combination of TDZ and NAA or BA and NAA can increase the yield of the total polysaccharides.The polysaccharides isolated from callus and whole wild plants had stronger free radicals scavenging activities,indicating that polysaccharides from R.dumulosa are the potential pharmaceutical supplements.展开更多
In the present study, free fatty acids(FFAs, including palmitic acid, stearic acid, oleic acid, linoleic acid and arachidonic acid) in rabbit plasma were determined by gas chromatography-mass spectrometry(GC-MS) after...In the present study, free fatty acids(FFAs, including palmitic acid, stearic acid, oleic acid, linoleic acid and arachidonic acid) in rabbit plasma were determined by gas chromatography-mass spectrometry(GC-MS) after trimethylsilylation derivatization using N,O-bis(trimethylsilyl)trifluoroacetamide(BSTFA) – trimethylchlorosilane(TMCS) as derivatization reagent. The experimental conditions, including extraction and silylation reaction, were investigated. The method was experimentally validated. The linearity between fatty acids’ peak areas and their concentrations was obtained with the corelative coefficient(r2) all more than 0.999, and the recoveries were between 82% and 111%. The intra-day variations of FFAs’ in plasma samples at different concentrations were all less than 6%. FFA analysis results of 16 rabbit plasma samples showed that the method could be well applied in the determination of plasma samples in vivo. In contrast to the traditional method of FFA derivatization, the established trimethylsilylation method presented simplicity, high specificity, and completely free from the interference of the esterified fatty acid, such as triacylglyceride. The method could be applied for analyzing FFA profiles in the clinical laboratory or pharmacological research.展开更多
基金This study was financially supported by the Independent Research Project of Graduate Students(No.BZKY2021035).
文摘Objective:As a medicinal plant,the resource of Rhodiola dumulosa is deficient along with the large collection.For the protection and utilization of R.dumulosa,the influence of plant growth regulators(PGRs)on callus induction and adventitious shoots differentiation,polysaccharide production and the antioxidant activity were tested.Methods:Internodes of R.dumulosa were used as explants and cultured on MS medium plus different plant growth regulators(PGRs).The anti-oxidative activities of polysaccharides were evaluated using radical scavenging assays.Results:By response surface plot,0.85 mg/L N6-benzyladenine(BA),0.34 mg/L naphthaleneacetic acid(NAA)and 0.33 mg/L 2,4-dicholorophenoxyacetic acid(2,4-D)were the optimal factors for callus induction(90.03%)from internodes explants on MS medium.The fresh weight of green callus increased 47.26 fold,when callus was inoculated on MS+thidiazuron(TDZ)0.5 mg/L+NAA 2.0 mg/L.Adventitious buds regenerated from callus on the media of MS were fortified with BA 1.0 mg/L plus NAA 0.5 mg/L,and the induction rate was 40.00%.MS plus indole-3-butyric acid(IBA)1.0 mg/L produced the highest rooting rate with 10 to 15 roots in a length of 2–3 cm per shoot.The content of total polysaccharides in callus developed on MS+TDZ 0.5 mg/L+NAA 2.0 mg/L and MS+BA 1.0 mg/L+NAA 0.5 mg/L was as high as 1.72%2.15%.At the dose of 0.5 mg/mL polysaccharides extracted from different callus induced on MS+NAA 2.0 mg/L+TDZ 0.5 mg/L or MS+BA 1.0 mg/L+NAA 0.5 mg/L or MS+BA 0.5 mg/L+2,4-D 0.5 mg/L,the ABTS radical eliminating percentages were 82.78%,80.18%and 68.59%,respectively,much higher than that of wild plant.Conclusion:A rapid micropropagation system for R.dumulosa has been developed.The combination of TDZ and NAA or BA and NAA can increase the yield of the total polysaccharides.The polysaccharides isolated from callus and whole wild plants had stronger free radicals scavenging activities,indicating that polysaccharides from R.dumulosa are the potential pharmaceutical supplements.
基金National Natural Science Foundation of China (Grant No. 31671928)Natural Science Foundation of Shanghai (Grant No. 15ZR1440800)。
文摘In the present study, free fatty acids(FFAs, including palmitic acid, stearic acid, oleic acid, linoleic acid and arachidonic acid) in rabbit plasma were determined by gas chromatography-mass spectrometry(GC-MS) after trimethylsilylation derivatization using N,O-bis(trimethylsilyl)trifluoroacetamide(BSTFA) – trimethylchlorosilane(TMCS) as derivatization reagent. The experimental conditions, including extraction and silylation reaction, were investigated. The method was experimentally validated. The linearity between fatty acids’ peak areas and their concentrations was obtained with the corelative coefficient(r2) all more than 0.999, and the recoveries were between 82% and 111%. The intra-day variations of FFAs’ in plasma samples at different concentrations were all less than 6%. FFA analysis results of 16 rabbit plasma samples showed that the method could be well applied in the determination of plasma samples in vivo. In contrast to the traditional method of FFA derivatization, the established trimethylsilylation method presented simplicity, high specificity, and completely free from the interference of the esterified fatty acid, such as triacylglyceride. The method could be applied for analyzing FFA profiles in the clinical laboratory or pharmacological research.
