With the continuous intensification of global aging,the issue of elderly care has become an increasingly prominent social problem.The Internet of Things(IoT)technology,as an emerging field,holds broad application pros...With the continuous intensification of global aging,the issue of elderly care has become an increasingly prominent social problem.The Internet of Things(IoT)technology,as an emerging field,holds broad application prospects.This article focuses on the application of IoT technology in group elderly care services and constructs a quality evaluation system for these services based on IoT technology.Through the analysis of practical application cases,the advantages and challenges of IoT technology in group elderly care services have been examined,confirming the feasibility and effectiveness of the evaluation system.展开更多
The integrity and fineness characterization of non-connected regions and contours is a major challenge for existing salient object detection.The key to address is how to make full use of the subjective and objective s...The integrity and fineness characterization of non-connected regions and contours is a major challenge for existing salient object detection.The key to address is how to make full use of the subjective and objective structural information obtained in different steps.Therefore,by simulating the human visual mechanism,this paper proposes a novel multi-decoder matching correction network and subjective structural loss.Specifically,the loss pays different attentions to the foreground,boundary,and background of ground truth map in a top-down structure.And the perceived saliency is mapped to the corresponding objective structure of the prediction map,which is extracted in a bottom-up manner.Thus,multi-level salient features can be effectively detected with the loss as constraint.And then,through the mapping of improved binary cross entropy loss,the differences between salient regions and objects are checked to pay attention to the error prone region to achieve excellent error sensitivity.Finally,through tracking the identifying feature horizontally and vertically,the subjective and objective interaction is maximized.Extensive experiments on five benchmark datasets demonstrate that compared with 12 state-of-the-art methods,the algorithm has higher recall and precision,less error and strong robustness and generalization ability,and can predict complete and refined saliency maps.展开更多
Elicitins are microbe-associated molecular patterns produced by oomycetes to elicit plant defense.It is still unclear whether elicitins derived from non-pathogenic oomycetes can be used as bioactive molecules for dise...Elicitins are microbe-associated molecular patterns produced by oomycetes to elicit plant defense.It is still unclear whether elicitins derived from non-pathogenic oomycetes can be used as bioactive molecules for disease control.Here,for the first time we identify and characterize an elicitin named PpEli2 from the soil-borne oomycete Pythium periplocum,which is a non-pathogenic mycoparasite colonizing the root ecosystem of diverse plant species.Perceived by a novel cell surface receptor-like protein,REli,that is conserved in various plants(e.g.tomato,pepper,soybean),PpEli2 can induce hypersensitive response cell death and an immunity response in Nicotiana benthamiana.Meanwhile,PpEli2 enhances the interaction between REli and its co-receptor BAK1.The receptor-dependent immune response triggered by PpEli2 is able to protect various plant species against Phytophthora and fungal infections.Collectively,our work reveals the potential agricultural application of non-pathogenic elicitins and their receptors in conferring broad-spectrum resistance for plant protection.展开更多
Internet of Things(IoT)technology is widely used in various fields,and its application in elderly care services has been highlighted in recent years.This study aims to explore how IoT technology can improve the effici...Internet of Things(IoT)technology is widely used in various fields,and its application in elderly care services has been highlighted in recent years.This study aims to explore how IoT technology can improve the efficiency of group-based elderly care services.The concept,characteristics,and current application status of IoT technology in elderly care services were introduced.Secondly,the characteristics and needs of group elderly care services were analyzed,including advantages and challenges,as well as the expectations and needs of the elderly for elderly care services.The evaluation methods and future development directions of IoT technology in improving the efficiency of group elderly care services were discussed,including data collection and analysis methods,selection and measurement of efficiency evaluation indicators,challenges,and development directions.展开更多
Twist provides a new degree of freedom for nanomaterial modifications,which can provide novel physical properties.Here,colloidal two-dimensional(2D)twisted CdSe nanoplatelets(NPLs)are successfully fabricated and their...Twist provides a new degree of freedom for nanomaterial modifications,which can provide novel physical properties.Here,colloidal two-dimensional(2D)twisted CdSe nanoplatelets(NPLs)are successfully fabricated and their morphology can change from totally flat to edge-twisted,and then to middle-twisted with prolonged reaction time.By combining experiments and corresponding theoretical analyses,we have established the length-dependent relationships between the surface energy and twist,with a critical lateral dimension of 30 nm.We found that the defects formed during the synthesis process play a vital role in generating intense stress that develops a strong torsion tensor around the edges,resulting in edge-twisted and final middletwisted NPLs.Furthermore,due to the geometric asymmetry of twisted NPLs,the dissymmetry factor of single particle NPLs can reach up to 0.334.Specifically,quantum coupling occurs in middle-twisted NPLs by twisting one parent NPL into two daughter NPLs,which are structurally and electronically coupled.This work not only further deepens our understanding of the twist mechanism of 2D NPLs during colloidal synthesis,but also opens a pathway for applications using twistronics and quantum technology.展开更多
The two frequently observed phenomena,photoluminescence(PL)blinking and quantum-confined Stark effect(QCSE)-induced spectral diffusion,are not conducive to the applications of colloidal quantum dots(QDs).It remains el...The two frequently observed phenomena,photoluminescence(PL)blinking and quantum-confined Stark effect(QCSE)-induced spectral diffusion,are not conducive to the applications of colloidal quantum dots(QDs).It remains elusive how these two phenomena are linked to each other.Unraveling the potential link between blinking and QCSE could facilitate the adoption of appropriate strategies that can simultaneously suppress both PL blinking and spectral diffusion.In this work,we investigated the blinking mechanism and QCSE of single CdSe/CdS/ZnS QDs in the presence of positive and negative surface charges using single-dot PL spectroscopy.We found that the negative surface charges can simultaneously suppress PL blinking and spectral diffusion of single QDs.On the other hand,the positive surface charges could change the blinking mechanisms of QDs from Auger-blinking to band-edge carrier(BC)-blinking.Two types of QCSE were observed,and a significant QCSE-induced spectral broadening of 5.25 nm was measured,which could be attributed to the hopping of surface charges between different surface-trap sites.Based on these findings,several theoretical models are proposed to explain various phenomena observed.展开更多
Group 2 innate lymphoid cells(ILC2s)play important tissue resident roles in anti-parasite immunity,allergic immune response,tissue homeostasis,and tumor immunity.ILC2s are considered tissue resident cells with little ...Group 2 innate lymphoid cells(ILC2s)play important tissue resident roles in anti-parasite immunity,allergic immune response,tissue homeostasis,and tumor immunity.ILC2s are considered tissue resident cells with little proliferation at steady state.Recent studies have shown that a subset of small intestinal ILC2s could leave their residing tissues,circulate and migrate to different organs,including lung,liver,mesenteric LN and spleen,upon activation.However,it remains unknown whether other ILC populations with migratory behavior exist.In this study,we find two major colon ILC2 populations with potential to migrate to the lung in response to IL-25 stimulation.One subset expresses IL-17A and resembles inflammatory ILC2s(iILC2s)but lacks CD27 expression,whereas the other expresses CD27 but not IL-17A.In addition,the IL-17A^(+)ILC2s express lower levels of CD127,CD25,and ST2 than CD27^(+)ILC2s,which express higher levels of IL-5 and IL-13.Surprisingly,we found that both colon ILC2 populations still maintained their colonic features of preferential expression of IL-17A and CD27,IL-5/IL-13,respectively.Together,our study identifies two migratory colon ILC2 subsets with unique surface markers and cytokine profiles which are critical in regulating lung and colon immunity and homeostasis.展开更多
The distance-based regression model has many applications in analysis of multivariate response regression in various ?elds, such as ecology, genomics, genetics, human microbiomics, and neuroimaging. It yields a pseudo...The distance-based regression model has many applications in analysis of multivariate response regression in various ?elds, such as ecology, genomics, genetics, human microbiomics, and neuroimaging. It yields a pseudo F test statistic that assesses the relation between the distance(dissimilarity) of the subjects and the predictors of interest. Despite its popularity in recent decades, the statistical properties of the pseudo F test statistic have not been revealed to our knowledge. This study derives the asymptotic properties of the pseudo F test statistic using spectral decomposition under the matrix normal assumption, when the utilized dissimilarity measure is the Euclidean or Mahalanobis distance. The pseudo F test statistic with the Euclidean distance has the same distribution as the quotient of two Chi-squared-type mixtures. The denominator and numerator of the quotient are approximated using a random variable of the form ξχ_d^2+ η, and the approximate error bound is given. The pseudo F test statistic with the Mahalanobis distance follows an F distribution.In simulation studies, the approximated distribution well matched the "exact" distribution obtained by the permutation procedure. The obtained distribution was further validated on H1N1 in?uenza data, aging human brain data, and embryonic imprint data.展开更多
Reducing crop loss to diseases is urgently needed to meet increasing food production challenges caused by the expanding world population and the negative impact of climate change on crop productivity.Disease-resistant...Reducing crop loss to diseases is urgently needed to meet increasing food production challenges caused by the expanding world population and the negative impact of climate change on crop productivity.Disease-resistant crops can be created by expressing endogenous or exogenous genes of interest through transgenic technology.Nevertheless,enhanced resistance by overexpressing resistance-produced genes often results in adverse developmental affects.Upstream open reading frames(uORFs)are translational control elements located in the 5′untranslated region(UTR)of eukaryotic mRNAs and may repress the translation of downstream genes.To investigate the function of three uORFs from the 5′-UTR of ACCELERATED CELL 11(uORFsACD11),we develop a fluorescent reporter system and find uORFsACD11 function in repressing downstream gene translation.Individual or simultaneous mutations of the three uORFsACD11 lead to repression of downstream translation efficiency at different levels.Importantly,uORFsACD11-mediated translational inhibition is impaired upon recognition of pathogen attack of plant leaves.When coupled with the PATHOGENESIS-RELATED GENE 1(PR1)promoter,the uORFsACD11 cassettes can upregulate accumulation of Arabidopsis thaliana LECTIN RECEPTOR KINASE-VI.2(AtLecRKVI.2)during pathogen attack and enhance plant resistance to Phytophthora capsici.These findings indicate that the uORFsACD11 cassettes can be a useful toolkit that enables a high level of protein expression during pathogen attack,while for ensuring lower levels of protein expression at normal conditions.展开更多
基金Phased Achievement of the National College Student Innovation and Entrepreneurship Training Project“Time Bay-A Group Elderly Care Service Platform Based on Internet of Things Technology”(S202013836008X)2021 Chongqing Education Commission Science and Technology Research Program Youth Project(KJQN202105501).
文摘With the continuous intensification of global aging,the issue of elderly care has become an increasingly prominent social problem.The Internet of Things(IoT)technology,as an emerging field,holds broad application prospects.This article focuses on the application of IoT technology in group elderly care services and constructs a quality evaluation system for these services based on IoT technology.Through the analysis of practical application cases,the advantages and challenges of IoT technology in group elderly care services have been examined,confirming the feasibility and effectiveness of the evaluation system.
基金supported by the National Natural Science Foundation of China(No.52174021)Key Research and Develop-ment Project of Hainan Province(No.ZDYF2022GXJS 003).
文摘The integrity and fineness characterization of non-connected regions and contours is a major challenge for existing salient object detection.The key to address is how to make full use of the subjective and objective structural information obtained in different steps.Therefore,by simulating the human visual mechanism,this paper proposes a novel multi-decoder matching correction network and subjective structural loss.Specifically,the loss pays different attentions to the foreground,boundary,and background of ground truth map in a top-down structure.And the perceived saliency is mapped to the corresponding objective structure of the prediction map,which is extracted in a bottom-up manner.Thus,multi-level salient features can be effectively detected with the loss as constraint.And then,through the mapping of improved binary cross entropy loss,the differences between salient regions and objects are checked to pay attention to the error prone region to achieve excellent error sensitivity.Finally,through tracking the identifying feature horizontally and vertically,the subjective and objective interaction is maximized.Extensive experiments on five benchmark datasets demonstrate that compared with 12 state-of-the-art methods,the algorithm has higher recall and precision,less error and strong robustness and generalization ability,and can predict complete and refined saliency maps.
基金supported by the National Natural Science Foundation of China(32272495,31801715,31721004)the Natural Science Foundation of Jiangsu Province(BK20220147).
文摘Elicitins are microbe-associated molecular patterns produced by oomycetes to elicit plant defense.It is still unclear whether elicitins derived from non-pathogenic oomycetes can be used as bioactive molecules for disease control.Here,for the first time we identify and characterize an elicitin named PpEli2 from the soil-borne oomycete Pythium periplocum,which is a non-pathogenic mycoparasite colonizing the root ecosystem of diverse plant species.Perceived by a novel cell surface receptor-like protein,REli,that is conserved in various plants(e.g.tomato,pepper,soybean),PpEli2 can induce hypersensitive response cell death and an immunity response in Nicotiana benthamiana.Meanwhile,PpEli2 enhances the interaction between REli and its co-receptor BAK1.The receptor-dependent immune response triggered by PpEli2 is able to protect various plant species against Phytophthora and fungal infections.Collectively,our work reveals the potential agricultural application of non-pathogenic elicitins and their receptors in conferring broad-spectrum resistance for plant protection.
基金National Innovation and Entrepreneurship Training Project“Time Bay-A Group Elderly Care Service Platform Based on Internet of Things Technology”(S202013836008X)Chongqing Education Commission Science and Technology Research Program Youth Project 2021(KJQN202105501)。
文摘Internet of Things(IoT)technology is widely used in various fields,and its application in elderly care services has been highlighted in recent years.This study aims to explore how IoT technology can improve the efficiency of group-based elderly care services.The concept,characteristics,and current application status of IoT technology in elderly care services were introduced.Secondly,the characteristics and needs of group elderly care services were analyzed,including advantages and challenges,as well as the expectations and needs of the elderly for elderly care services.The evaluation methods and future development directions of IoT technology in improving the efficiency of group elderly care services were discussed,including data collection and analysis methods,selection and measurement of efficiency evaluation indicators,challenges,and development directions.
基金This work was financially supported by the Beijing Natural Science Foundation(No.Z210018)the National Natural Science Foundation of China(Nos.62105025,12172047,62127817,and 22173009)the Beijing Institute of Technology Research Fund Program for Young Scholars(No.3040011182113)。
文摘Twist provides a new degree of freedom for nanomaterial modifications,which can provide novel physical properties.Here,colloidal two-dimensional(2D)twisted CdSe nanoplatelets(NPLs)are successfully fabricated and their morphology can change from totally flat to edge-twisted,and then to middle-twisted with prolonged reaction time.By combining experiments and corresponding theoretical analyses,we have established the length-dependent relationships between the surface energy and twist,with a critical lateral dimension of 30 nm.We found that the defects formed during the synthesis process play a vital role in generating intense stress that develops a strong torsion tensor around the edges,resulting in edge-twisted and final middletwisted NPLs.Furthermore,due to the geometric asymmetry of twisted NPLs,the dissymmetry factor of single particle NPLs can reach up to 0.334.Specifically,quantum coupling occurs in middle-twisted NPLs by twisting one parent NPL into two daughter NPLs,which are structurally and electronically coupled.This work not only further deepens our understanding of the twist mechanism of 2D NPLs during colloidal synthesis,but also opens a pathway for applications using twistronics and quantum technology.
基金the National Key Research and Development Program of China(No.2017YFA0304203)the National Natural Science Foundation of China(Nos.62127817,62075120,62075122,61875109,91950109,and 62105193),NSFCSTINT(No.62011530133)+3 种基金PCSIRT(No.IRT_17R70)Natural Science Foundation of Shanxi Province(No.201901D111010(ZD))Research Project Supported by Shanxi Scholarship Council of China(No.HGKY2019002)PTIT,Shanxi“1331 Project”,and 111 project(No.D18001).
文摘The two frequently observed phenomena,photoluminescence(PL)blinking and quantum-confined Stark effect(QCSE)-induced spectral diffusion,are not conducive to the applications of colloidal quantum dots(QDs).It remains elusive how these two phenomena are linked to each other.Unraveling the potential link between blinking and QCSE could facilitate the adoption of appropriate strategies that can simultaneously suppress both PL blinking and spectral diffusion.In this work,we investigated the blinking mechanism and QCSE of single CdSe/CdS/ZnS QDs in the presence of positive and negative surface charges using single-dot PL spectroscopy.We found that the negative surface charges can simultaneously suppress PL blinking and spectral diffusion of single QDs.On the other hand,the positive surface charges could change the blinking mechanisms of QDs from Auger-blinking to band-edge carrier(BC)-blinking.Two types of QCSE were observed,and a significant QCSE-induced spectral broadening of 5.25 nm was measured,which could be attributed to the hopping of surface charges between different surface-trap sites.Based on these findings,several theoretical models are proposed to explain various phenomena observed.
基金supported by the National Natural Science Foundation of China(31930035,91942311,32061143028)Shanghai Science and Technology Commission(20410714000)+1 种基金the National Key Research and Developmental Program of China(2021YFA1301400)generous support from Flow cytometry Core and Sequencing Core at Shanghai Institute of Immunology and animal facility of Shanghai Jiao Tong University School of Medicine。
文摘Group 2 innate lymphoid cells(ILC2s)play important tissue resident roles in anti-parasite immunity,allergic immune response,tissue homeostasis,and tumor immunity.ILC2s are considered tissue resident cells with little proliferation at steady state.Recent studies have shown that a subset of small intestinal ILC2s could leave their residing tissues,circulate and migrate to different organs,including lung,liver,mesenteric LN and spleen,upon activation.However,it remains unknown whether other ILC populations with migratory behavior exist.In this study,we find two major colon ILC2 populations with potential to migrate to the lung in response to IL-25 stimulation.One subset expresses IL-17A and resembles inflammatory ILC2s(iILC2s)but lacks CD27 expression,whereas the other expresses CD27 but not IL-17A.In addition,the IL-17A^(+)ILC2s express lower levels of CD127,CD25,and ST2 than CD27^(+)ILC2s,which express higher levels of IL-5 and IL-13.Surprisingly,we found that both colon ILC2 populations still maintained their colonic features of preferential expression of IL-17A and CD27,IL-5/IL-13,respectively.Together,our study identifies two migratory colon ILC2 subsets with unique surface markers and cytokine profiles which are critical in regulating lung and colon immunity and homeostasis.
基金This work is partially supported by Shanghai 2020“Science and Technology Innovation Action Plan”for Social Development Science and Technology Research Project under the project number of 20DZ1207802。
基金This work was supported by grants from the National research platform of clinical evaluation technology for new anticancer drugs （No. 2013ZX09303001 ）, the National Natural Science Foundation of China （Grant Nos. 81201946 and 81372394） and Tianjin City High School Science ＆ Technology Fund Planning Project （20130122）. The funders had no role in study design collection, analysis, and interpretation of data+1 种基金 in the writing of the report and in the decision to submit this article for publication.
基金supported by National Natural Science Foundation of China (Grant No. 11722113)
文摘The distance-based regression model has many applications in analysis of multivariate response regression in various ?elds, such as ecology, genomics, genetics, human microbiomics, and neuroimaging. It yields a pseudo F test statistic that assesses the relation between the distance(dissimilarity) of the subjects and the predictors of interest. Despite its popularity in recent decades, the statistical properties of the pseudo F test statistic have not been revealed to our knowledge. This study derives the asymptotic properties of the pseudo F test statistic using spectral decomposition under the matrix normal assumption, when the utilized dissimilarity measure is the Euclidean or Mahalanobis distance. The pseudo F test statistic with the Euclidean distance has the same distribution as the quotient of two Chi-squared-type mixtures. The denominator and numerator of the quotient are approximated using a random variable of the form ξχ_d^2+ η, and the approximate error bound is given. The pseudo F test statistic with the Mahalanobis distance follows an F distribution.In simulation studies, the approximated distribution well matched the "exact" distribution obtained by the permutation procedure. The obtained distribution was further validated on H1N1 in?uenza data, aging human brain data, and embryonic imprint data.
基金the National Natural Science Foundation of China(31625023,31721004,and 32072507)the Fundamental Research Funds for the Central Universities(KYT202001).
文摘Reducing crop loss to diseases is urgently needed to meet increasing food production challenges caused by the expanding world population and the negative impact of climate change on crop productivity.Disease-resistant crops can be created by expressing endogenous or exogenous genes of interest through transgenic technology.Nevertheless,enhanced resistance by overexpressing resistance-produced genes often results in adverse developmental affects.Upstream open reading frames(uORFs)are translational control elements located in the 5′untranslated region(UTR)of eukaryotic mRNAs and may repress the translation of downstream genes.To investigate the function of three uORFs from the 5′-UTR of ACCELERATED CELL 11(uORFsACD11),we develop a fluorescent reporter system and find uORFsACD11 function in repressing downstream gene translation.Individual or simultaneous mutations of the three uORFsACD11 lead to repression of downstream translation efficiency at different levels.Importantly,uORFsACD11-mediated translational inhibition is impaired upon recognition of pathogen attack of plant leaves.When coupled with the PATHOGENESIS-RELATED GENE 1(PR1)promoter,the uORFsACD11 cassettes can upregulate accumulation of Arabidopsis thaliana LECTIN RECEPTOR KINASE-VI.2(AtLecRKVI.2)during pathogen attack and enhance plant resistance to Phytophthora capsici.These findings indicate that the uORFsACD11 cassettes can be a useful toolkit that enables a high level of protein expression during pathogen attack,while for ensuring lower levels of protein expression at normal conditions.
