Despite its dual role in determining cell fate in a wide array of solid cancer cell lines, autophagy has been robustly shown to suppress or kill acute myeloid leukemia cells via degradation of the oncogenic fusion pro...Despite its dual role in determining cell fate in a wide array of solid cancer cell lines, autophagy has been robustly shown to suppress or kill acute myeloid leukemia cells via degradation of the oncogenic fusion protein that drives leukemogenesis. However, autophagy also induces the demise of acute leukemia cells that do not express the known fusion protein, though the molecular mechanism remains elusive. Nevertheless, since it can induce cooperation with apoptosis and differentiation in response to autophagic signals, autophagy can be manipulated for a better therapy on acute myeloid leukemia.展开更多
AIM: To investigate the changes of retinal thickness in macula of high myopic eyes using spectral domain optical coherence tomography(OCT). ·METHODS: Middle-aged and young myopic patients were divided into three ...AIM: To investigate the changes of retinal thickness in macula of high myopic eyes using spectral domain optical coherence tomography(OCT). ·METHODS: Middle-aged and young myopic patients were divided into three groups according to their refractive error/axial length: low and medium myopia group(LMMG),high myopia group(HMG) and super high myopia group(SHMG). Cirrus HD-OCT was used to evaluate total average macular thickness,central subfield thickness,inner/outer macular thickness and macular volume. The differences among experimental groups were analyzed by one-factor analysis of variance. Associations between macular thickness and refractive error/axial length were analyzed by Pearson correlation analysis. ·RESULTS: There was no significant difference in age among the three groups(P =0.2789). The mean refraction error in the LMMG,HMG,and SHMG groups was-2.49 ± 1.38D,-8.53 ±1.95D and-13.88 ±1.76D,respectively(P 【 0.001). The central subfield thickness of three groups was 244.56 ±12.19μm,254.33 ±11.61μm and 261.75 ± 11.83μm,respectively,and there were statistically significance between random two groups. The total average macular thickness,inner/outer macular thickness,and macular volume decreased with increased myopia/axial length. Average foveal thickness had negative correlations with refractive error(P 【0.001),and positive correlations with axial length. The inferior and temporal inner macular thickness,all the quadrants of outer ring,total average macular thickness and macular volume featured positive correlations with refractive error,and negative correlations with axial length. Average foveal thickness,superior and temporal innermacular thicknesses,and temporal outer macular thickness was lower in females compared to males. ·CONCLUSION: With an increase in myopia degree/axial length,the average foveal thickness increased and the inner/outer macular thickness decreased. Females featured thicker average foveal thickness,and thinner macular thickness compared to males.展开更多
AIM： To investigate the changes of retinal function in non-pathological myopic subjects using multifocal electroretinography （mfERG） and to correlate the data with the central macular thickness obtained using optic...AIM： To investigate the changes of retinal function in non-pathological myopic subjects using multifocal electroretinography （mfERG） and to correlate the data with the central macular thickness obtained using optical coherence tomography （OCT）. ~ METHODS： One hundred and thirteen subjects （113 eyes） with age range from 18 to 35y were enrolled in the study. The subjects were divided into four groups according to spherical equivalent （SE） and axial length （AL）： emmetropia group （EG, n=31; SE： ＋0.75 to -0.50 D; AL： 22 to 24 mm）, low and medium myopia group （LMMG, n=26; SE： 〉-0.50 to -6.00 D; AL： 〉24 to 26 ram）, high myopia group （HMG, n--34; SE： 〉-6.00 to -10.00 D; AL： 〉26 to 28 mm） and super high myopia group （SHMG, n =22; SE： 〉-10.00 D; AL： 〉28 mm）. The P1 amplitude density, P1 amplitude, and P1 implicit time of the first- order kernel mfERG responses were obtained and grouped into five rings. The central subfield macular thickness （CST） was obtained using macular cube 512x218 scan of Cirrus HD-OCT. RESULTS： With the increasing of eccentricity, the first positive peak （P1） amplitude density （P=0.0000, 0.0001, 0.0021 in ring 1-3 respectively） and P1 amplitude （all P= 0.0000 in ring 1-5） of each group decreased. With the increasing of myopia, P1 implicit time gradually extended （all P=0.0000 in ring 1-3）. The average CST in four diagnostic groups was 241.56±12.72um, 244.56±12.19um, 254.33±11.61um, 261.75±11.83um respectively. With the increasing of myopia, CST increased （P〈0.001）. There was negative relationship between CST and P1 amplitude, P1 amplitude density （r =-0.402, P〈0.001; r= -0.261, P =0.003）. There was positive relationship between CST and P1 implicit time （r=0.34, P〈0.001）.~ CONCLUSION： With the increasing of myopia, P1 amplitude density and P1 amplitude of the first-order reaction gradually reduced. This showed potential decline in retinal function in myopia. To some extent it may reflect the functional disorder or depression of the visual cells, The exact mechanism needs further study,展开更多
As a classical complex network model, scale-free network is widely used and studied. And motifs, as a high-order subgraph structure, frequently appear in scale-free networks, and have a great influence on the structur...As a classical complex network model, scale-free network is widely used and studied. And motifs, as a high-order subgraph structure, frequently appear in scale-free networks, and have a great influence on the structural integrity, functional integrity and dynamics of the networks. In order to overcome the shortcomings in the existing work on the robustness of complex networks, only nodes or edges are considered, while the defects of high-order structure in the network are ignored.From the perspective of network motif, we propose an entropy of node degree distribution based on motif to measure the robustness of scale-free networks under random attacks. The effectiveness and superiority of our method are verified and analyzed in the BA scale-free networks.展开更多
A detailed chemical study on the Hainan soft coral Sinularia crass has resulted in the isolation and characterization of eleven cas-bane-type diterpenoids,named sinucrassins A-K(1-11),along with six known related ones...A detailed chemical study on the Hainan soft coral Sinularia crass has resulted in the isolation and characterization of eleven cas-bane-type diterpenoids,named sinucrassins A-K(1-11),along with six known related ones(12-17).Their structures were elucidated by extensive spectroscopic analyses and comparison with the reported data.The absolute configurations of new compounds were determi ned by the X-ray diffracti on analysis and computer-assisted structural elucidati on in eluding 13C NMR data calculati on and time-dependent density functional theory/electronic circular dichroism calculation.展开更多
A detailed chemical investigation of the South China Sea nudibranch Hexabranchus sanguineus,as well as its possible sponge-prey Acanthella cavernosa,led to the isolation of fifteen new nitrogenous sesquiterpenoids,nam...A detailed chemical investigation of the South China Sea nudibranch Hexabranchus sanguineus,as well as its possible sponge-prey Acanthella cavernosa,led to the isolation of fifteen new nitrogenous sesquiterpenoids,namely ximaocavernosins A-0,including seven cadinanes(1-7),seven spiroaxanes(12-18)and one aromadendrane(19),together with thirteen known related compounds(8-11 and 20-28).展开更多
One new dolabellane-type diterpenoid.named clavirolide C（1）,and one known related analogue（3）.have been isolated from the soft coral Cavularia viridis collected off the Xisha Islands in the South China Sea.Its str...One new dolabellane-type diterpenoid.named clavirolide C（1）,and one known related analogue（3）.have been isolated from the soft coral Cavularia viridis collected off the Xisha Islands in the South China Sea.Its structure and absolute configuration were determined on the basis of spectroscopic analysis,electronic circular dichroism.and compared with literature model compounds.The cytotoxic activity of these two compounds was evaluated against a panel of human tumor cell lines.Compound 1 showed moderate cytotoxic activity against KB and HL-60 cells.展开更多
In the present study,nine new cembrane-type diterpenoids,namely ximaonanolobatins A—I(1—9),together with six related known analogs(10—15)and four known tetraprenylated alkaloids malonganenones D,E,H,and K(16—19)we...In the present study,nine new cembrane-type diterpenoids,namely ximaonanolobatins A—I(1—9),together with six related known analogs(10—15)and four known tetraprenylated alkaloids malonganenones D,E,H,and K(16—19)were isolated from the soft coral Sinularia nanolobata collected off the Ximao Island in the South China Sea.The structures of 1—9 were robustly established by a combination of detailed spectroscopic analyses,chemical reactions,quantum mechanical(QM)-NMR methods,biogenetic consideration,and the comparison with those literature data.The absolute configuration of 1 was confirmed by X-ray diffraction analysis,and the absolute configuration of 2 was determined by QM-NMR calculations and chemical transformation.In addition,the absolute configuration of 3 was determined using the modified Mosher's method.All these isolates were evaluated for their anti-inflammatory and anti-tumor activities.The results showed that only compound 15 exhibited potential anti-inflammatory effect against LPS-induced TNF-αrelease in RAW264.7 macrophages with an IC50 value of 12.6μmol/L.展开更多
基金supported by grants from National Science Foundation of China (No. 31071258)The Ministry of Science and Technology of China (Pre-973 Plan: No. 2011CB512101+1 种基金 863 Plan: No. 2011AA020114)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
文摘Despite its dual role in determining cell fate in a wide array of solid cancer cell lines, autophagy has been robustly shown to suppress or kill acute myeloid leukemia cells via degradation of the oncogenic fusion protein that drives leukemogenesis. However, autophagy also induces the demise of acute leukemia cells that do not express the known fusion protein, though the molecular mechanism remains elusive. Nevertheless, since it can induce cooperation with apoptosis and differentiation in response to autophagic signals, autophagy can be manipulated for a better therapy on acute myeloid leukemia.
文摘AIM: To investigate the changes of retinal thickness in macula of high myopic eyes using spectral domain optical coherence tomography(OCT). ·METHODS: Middle-aged and young myopic patients were divided into three groups according to their refractive error/axial length: low and medium myopia group(LMMG),high myopia group(HMG) and super high myopia group(SHMG). Cirrus HD-OCT was used to evaluate total average macular thickness,central subfield thickness,inner/outer macular thickness and macular volume. The differences among experimental groups were analyzed by one-factor analysis of variance. Associations between macular thickness and refractive error/axial length were analyzed by Pearson correlation analysis. ·RESULTS: There was no significant difference in age among the three groups(P =0.2789). The mean refraction error in the LMMG,HMG,and SHMG groups was-2.49 ± 1.38D,-8.53 ±1.95D and-13.88 ±1.76D,respectively(P 【 0.001). The central subfield thickness of three groups was 244.56 ±12.19μm,254.33 ±11.61μm and 261.75 ± 11.83μm,respectively,and there were statistically significance between random two groups. The total average macular thickness,inner/outer macular thickness,and macular volume decreased with increased myopia/axial length. Average foveal thickness had negative correlations with refractive error(P 【0.001),and positive correlations with axial length. The inferior and temporal inner macular thickness,all the quadrants of outer ring,total average macular thickness and macular volume featured positive correlations with refractive error,and negative correlations with axial length. Average foveal thickness,superior and temporal innermacular thicknesses,and temporal outer macular thickness was lower in females compared to males. ·CONCLUSION: With an increase in myopia degree/axial length,the average foveal thickness increased and the inner/outer macular thickness decreased. Females featured thicker average foveal thickness,and thinner macular thickness compared to males.
文摘AIM： To investigate the changes of retinal function in non-pathological myopic subjects using multifocal electroretinography （mfERG） and to correlate the data with the central macular thickness obtained using optical coherence tomography （OCT）. ~ METHODS： One hundred and thirteen subjects （113 eyes） with age range from 18 to 35y were enrolled in the study. The subjects were divided into four groups according to spherical equivalent （SE） and axial length （AL）： emmetropia group （EG, n=31; SE： ＋0.75 to -0.50 D; AL： 22 to 24 mm）, low and medium myopia group （LMMG, n=26; SE： 〉-0.50 to -6.00 D; AL： 〉24 to 26 ram）, high myopia group （HMG, n--34; SE： 〉-6.00 to -10.00 D; AL： 〉26 to 28 mm） and super high myopia group （SHMG, n =22; SE： 〉-10.00 D; AL： 〉28 mm）. The P1 amplitude density, P1 amplitude, and P1 implicit time of the first- order kernel mfERG responses were obtained and grouped into five rings. The central subfield macular thickness （CST） was obtained using macular cube 512x218 scan of Cirrus HD-OCT. RESULTS： With the increasing of eccentricity, the first positive peak （P1） amplitude density （P=0.0000, 0.0001, 0.0021 in ring 1-3 respectively） and P1 amplitude （all P= 0.0000 in ring 1-5） of each group decreased. With the increasing of myopia, P1 implicit time gradually extended （all P=0.0000 in ring 1-3）. The average CST in four diagnostic groups was 241.56±12.72um, 244.56±12.19um, 254.33±11.61um, 261.75±11.83um respectively. With the increasing of myopia, CST increased （P〈0.001）. There was negative relationship between CST and P1 amplitude, P1 amplitude density （r =-0.402, P〈0.001; r= -0.261, P =0.003）. There was positive relationship between CST and P1 implicit time （r=0.34, P〈0.001）.~ CONCLUSION： With the increasing of myopia, P1 amplitude density and P1 amplitude of the first-order reaction gradually reduced. This showed potential decline in retinal function in myopia. To some extent it may reflect the functional disorder or depression of the visual cells, The exact mechanism needs further study,
基金Project supported by the National Natural Science Foundation of China (Grant No. 62006169)the Youth Natural Science Foundation of Shanxi Province, China (Grant No. 201901D211304)+1 种基金the China Postdoctoral Science Foundation (Grant No. 2021M692400)the Science and Technology Innovation Projects of Universities in Shanxi Province, China (Grant No. 2020L0021)。
文摘As a classical complex network model, scale-free network is widely used and studied. And motifs, as a high-order subgraph structure, frequently appear in scale-free networks, and have a great influence on the structural integrity, functional integrity and dynamics of the networks. In order to overcome the shortcomings in the existing work on the robustness of complex networks, only nodes or edges are considered, while the defects of high-order structure in the network are ignored.From the perspective of network motif, we propose an entropy of node degree distribution based on motif to measure the robustness of scale-free networks under random attacks. The effectiveness and superiority of our method are verified and analyzed in the BA scale-free networks.
基金supported by the National Natural Science Foundation of China(No.81991521)the National Key Research and Development Program of China(No.2018YFC0310903)the SKLDR/SIMM Project(No.SIMM2103ZZ-06)。
文摘A detailed chemical study on the Hainan soft coral Sinularia crass has resulted in the isolation and characterization of eleven cas-bane-type diterpenoids,named sinucrassins A-K(1-11),along with six known related ones(12-17).Their structures were elucidated by extensive spectroscopic analyses and comparison with the reported data.The absolute configurations of new compounds were determi ned by the X-ray diffracti on analysis and computer-assisted structural elucidati on in eluding 13C NMR data calculati on and time-dependent density functional theory/electronic circular dichroism calculation.
基金This research work was financially supported by the National Natural Science Foundation of China(Nos.81991521,82022069,42076099)the Shanghai Rising-Star Program(No.20QA1411100)+1 种基金"Youth Innovation Promotion Association"of Chinese Academy of Sciences(No.Y202065)the SKLDR/SIMM Project(No.SIMM2103ZZ-06).
文摘A detailed chemical investigation of the South China Sea nudibranch Hexabranchus sanguineus,as well as its possible sponge-prey Acanthella cavernosa,led to the isolation of fifteen new nitrogenous sesquiterpenoids,namely ximaocavernosins A-0,including seven cadinanes(1-7),seven spiroaxanes(12-18)and one aromadendrane(19),together with thirteen known related compounds(8-11 and 20-28).
基金financially supported by the National Natural Science Foundation of China(Nos.81520108028,21672230,41506187,816013016)the SKLDR/SIMM Project(No.SIMM1501ZZ-03)
文摘One new dolabellane-type diterpenoid.named clavirolide C（1）,and one known related analogue（3）.have been isolated from the soft coral Cavularia viridis collected off the Xisha Islands in the South China Sea.Its structure and absolute configuration were determined on the basis of spectroscopic analysis,electronic circular dichroism.and compared with literature model compounds.The cytotoxic activity of these two compounds was evaluated against a panel of human tumor cell lines.Compound 1 showed moderate cytotoxic activity against KB and HL-60 cells.
基金financially supported by the National Key Research and Development Program of China(No.2018YFC0310903)the Natural Science Foundation of China(No.81991521)the SKLDR/SIMM Project(SIMM2103ZZ-06).
文摘In the present study,nine new cembrane-type diterpenoids,namely ximaonanolobatins A—I(1—9),together with six related known analogs(10—15)and four known tetraprenylated alkaloids malonganenones D,E,H,and K(16—19)were isolated from the soft coral Sinularia nanolobata collected off the Ximao Island in the South China Sea.The structures of 1—9 were robustly established by a combination of detailed spectroscopic analyses,chemical reactions,quantum mechanical(QM)-NMR methods,biogenetic consideration,and the comparison with those literature data.The absolute configuration of 1 was confirmed by X-ray diffraction analysis,and the absolute configuration of 2 was determined by QM-NMR calculations and chemical transformation.In addition,the absolute configuration of 3 was determined using the modified Mosher's method.All these isolates were evaluated for their anti-inflammatory and anti-tumor activities.The results showed that only compound 15 exhibited potential anti-inflammatory effect against LPS-induced TNF-αrelease in RAW264.7 macrophages with an IC50 value of 12.6μmol/L.
