AIM： To investigate the effects of electrical stimulation of hypothalamic paraventricular nuclei （PVN） on gastric mucosal cellular apoptosis and proliferation induced by gastric ischemia/reperfusion （I/R） injury....AIM： To investigate the effects of electrical stimulation of hypothalamic paraventricular nuclei （PVN） on gastric mucosal cellular apoptosis and proliferation induced by gastric ischemia/reperfusion （I/R） injury. METHODS： For different experimental purposes, stimulating electrode plantation or electrolytic destruction of the PVN was applied, then the animals＇ GI/R injury model was established by clamping the celiac artery for 30 min and allowing reperfusing the artery for 30 rain, 1 h, 3 h or 6 h respectively. Then histological, immunohistochemistry methods were used to assess the gastric mucosal damage index, the gastric mucosal cellular apoptosis and proliferation at different times. RESULTS： The electrical stimulation of PVN significantly attenuated the GI/R injury at 30 min, i h and 3 h after reperfusion. The electrical stimulation of PVN decreased gastric mucosal apoptosis and increased gastric mucosal proliferation. The electrolytic destruction of the PVN could eliminate the protective effects of electrical stimulation of PVN on GI/R injury. These results indicated that the PVN participated in the regulation of GI/R injury as a specific area in the brain, exerting protective effects against the GI/R injury, and the protection was associated with the inhibition of cellular apoptosis and the promotion of gastric mucosal proliferation. CONCLUSION： Stimulating PVN significantly inhibits the gastric mucosal cellular apoptosis and promots gastric mucosal cellular proliferation. This may explain the protective mechanisms of electrical stimulation of PVN against GI/R injury.展开更多
Textures of high-strength and low-expansion Fe-Ni alloy wires during cold-drawing processes were investigated using X-ray diffraction （XRD） and electron back scatter diffraction （EBSD） techniques. The experimental...Textures of high-strength and low-expansion Fe-Ni alloy wires during cold-drawing processes were investigated using X-ray diffraction （XRD） and electron back scatter diffraction （EBSD） techniques. The experimental results show that the 〈111〉 and 〈100〉 fibre textures are the main texture components, and crystalline grains in the surface are more fine and uniform than those in the center of Fe-Ni alloy wires during cold-drawing processes. It is found that the volume fraction of the 〈111〉 fibre texture component determined by quantitative regression calculation of the Gaussian distribution function reaches more than 60% and the strong 〈111〉 fibre texture component favors the torsional property of Fe-Ni alloy wires.展开更多
AIM: To investigate the protective effect and mechanisms of ghrelin postconditioning against hypoxia/reoxygenation (H/R)-induced injury in human gastric epithelial cells. METHODS: The model of H/R injury was establish...AIM: To investigate the protective effect and mechanisms of ghrelin postconditioning against hypoxia/reoxygenation (H/R)-induced injury in human gastric epithelial cells. METHODS: The model of H/R injury was established in gastric epithelial cell line (GES-1) human gastric epithelial cells. Cells were divided into seven groups: normal control group (N); H/R postconditioning group; DMSO postconditioning group (DM); ghrelin postconditioning group (GH); D-Lys3-GHRP-6 + ghrelin postconditioning group (D + GH); capsazepine + ghrelin postconditioning group (C + GH); and LY294002 + ghrelin postconditioning group (L + GH). 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to detect GES-1 cell viability. Hoechst 33258 fluorochrome staining and flow cytometry were conducted to determine apoptosis of GES-1cells. Spectrophotometry was performed to determine release of lactate dehydrogenate (LDH). Protein expression of Bcl-2, Bax, Akt, and glycogen synthase kinase (GSK)-3β was determined by western blotting. Expression of vanilloid receptor subtype 1 (VR1), Akt and GSK-3β was observed by immunocytochemistry. RESULTS: Compared with the H/R group, cell viability of the GH group was significantly increased in a dosedependent manner (55.9% ± 10.0% vs 69.6% ± 9.6%, 71.9% ± 17.4%, and 76.3% ± 13.3%). Compared with the H/R group, the percentage of apoptotic cells in the GH group significantly decreased (12.38% ± 1.51% vs 6.88% ± 0.87%). Compared with the GH group, the percentage of apoptotic cells in the D + GH group, C + GH group and L + GH groups significantly increased (11.70% ± 0.88%, 11.93% ± 0.96%, 10.20% ± 1.05% vs 6.88% ± 0.87%). There were no significant differences in the percentage of apoptotic cells between the H/R and DM groups (12.38% ± 1.51% vs 13.00% ± 1.13%). There was a significant decrease in LDH release following ghrelin postconditioning compared with the H/R group (561.58 ± 64.01 U/L vs 1062.45 ± 105.29 U/L). There was a significant increase in LDH release in the D + GH, C + GH and L + GH groups compared with the GH group (816.89 ± 94.87 U/L, 870.95 ± 64.06 U/L, 838.62 ± 118.45 U/L vs 561.58 ± 64.01 U/L). There were no significant differences in LDH release between the H/R and DM groups (1062.45 ± 105.29 U/L vs 1017.65 ± 68.90 U/L). Compared with the H/R group, expression of Bcl-2 and Akt increased in the GH group, whereas expression of Bax and GSK3β decreased. Compared with the GH group, expression of Bcl-2 decreased and Bax increased in the D + GH, C + GH and L + GH groups, and Akt decreased and GSK-3β increased in the L + GH group. The H/R group also upregulated expression of VR1 and GSK-3β and downregulated Akt. The number of VR1-positive and Akt-positive cells in the GH group significantly increased, whereas the number of GSK-3β-positive cells significantly decreased. These effects of ghrelin were reversed by capsazepine and LY294002.CONCLUSION: Ghrelin postconditioning protected against H/R-induced injury in human gastric epithelial cells, which indicated that this protection might be associated with GHS-R, VR1 and the PI3K/Akt signaling pathway.展开更多
This paper presents a hadronic dominated jet model to investigate multi- wavelength emission from the microquasar LS I ＋61 303. In this scenario, we take into account evolutions of the primary particles and secondary...This paper presents a hadronic dominated jet model to investigate multi- wavelength emission from the microquasar LS I ＋61 303. In this scenario, we take into account evolutions of the primary particles and secondary e± pairs; these pairs are produced by the collisional interactions of the accelerated protons with the cold jet protons and the stellar wind ions. In this model, the non-thermal photons are produced by πο decay emission, synchrotron and inverse Compton scattering processes from the primary electrons and secondary pairs, and relativistic bremsstrahlung emission from the secondary leptonic pairs. Based on this model framework, we show that the spectral energy distributions can be produced by the primary and secondary particles via interactions with the cold matter, and magnetic and stellar radiation fields. We also consider the attenuation of angular dependence γ-γ due to the effects of the stel- lar target photon fields. The resulting model can approximately reproduce the recent quasi-simultaneous observational data points and the non-simultaneous multi-band observations.展开更多
We study non-thermal emissions from cascade processes in accreting X-raybinary pulsars. In the framework of the magnetospheric gap model, we consider threephoton fields, which are respectively from the polar cap of a ...We study non-thermal emissions from cascade processes in accreting X-raybinary pulsars. In the framework of the magnetospheric gap model, we consider threephoton fields, which are respectively from the polar cap of a pulsar, its surroundingaccretion disk and a massive companion star with a circumstellar disk, to shield thegap. The gap-accelerated ultra-relativistic electrons emit high-energy photons via cur-vature radiation and an inverse Compton scattering process, in which part of thesehigh-energy photons absorbed by interactions with the surrounding photon fields canfacilitate the following electromagnetic cascades. We first carry out numerical calcula-tions of the cascade processes in order to obtain the predicted emission spectra. As anexample, we subsequently apply this model to reproduce observations of LS I ＋61~303. We find that the results can fit observations ranging from hard X-ray to γ-raybands, In particular, they can explain the spectral cutoff feature at a few GeV. Finally,we suggest that the emissions detected by the Fermi Large Area Telescope from X-raybinary pulsars originate in the magnetosphere region of the pulsar.展开更多
Image matting is to estimate the opacity of foreground objects from an image. A few deep learning based methods have been proposed for image matting and perform well in capturing spatially close information. However, ...Image matting is to estimate the opacity of foreground objects from an image. A few deep learning based methods have been proposed for image matting and perform well in capturing spatially close information. However, these methods fail to capture global contextual information, which has been proved essential in improving matting performance. This is because a matting image may be up to several megapixels, which is too big for a learning-based network to capture global contextual information due to the limit size of a receptive field. Although uniformly downsampling the matting image can alleviate this problem, it may result in the degradation of matting performance. To solve this problem, we introduce a natural image matting with the attended global context method to extract global contextual information from the whole image, and to condense them into a suitable size for learning-based network. Specifically, we first leverage a deformable sampling layer to obtain condensed foreground and background attended images respectively. Then, we utilize a contextual attention layer to extract information related to unknown regions from condensed foreground and background images generated by a deformable sampling layer. Besides, our network predicts a background as well as the alpha matte to obtain more purified foreground, which contributes to better qualitative performance in composition. Comprehensive experiments show that our method achieves competitive performance on both Composition-1k and the alphamatting.com benchmark quantitatively and qualitatively.展开更多
Mn-Cu alloys could exhibit high damping ability and excellent mechanical properties after proper heat treatment. In order to reduce the influence of impurity elements on damping capacity of Mn-Cu alloys, rare ele- men...Mn-Cu alloys could exhibit high damping ability and excellent mechanical properties after proper heat treatment. In order to reduce the influence of impurity elements on damping capacity of Mn-Cu alloys, rare ele- ment cerium （Ce） was added into MnCuNiFe alloys. It is indicated that the contents of C, S and Si which have adverse effects on the damping capacity decrease and the grains are refined with the Ce content increasing. The microstructure of the MnCuNiFeCe alloy was investigated by X-ray diffraction （XRD）, scanning electron microscope （SEM） and transmission electron microscopy （TEM）. The damping ability （tane） of the alloy was characterized by dynamical mechanical analyzer （DMA）. It is found that the damping ability （tane） retains a very high level which is all above 0.05 from the temperature of -50 to 75 ℃ with the addition of Ce element. It is expected that the Ce alloying MnCuNiFe alloy with refined grains could find wide applications in the field of industry.展开更多
The giant magnetostrictive rotary ultrasonic processing system(GMUPS)with a loosely-coupled contactless power transfer(LCCPT)has emerged as a high-performance technique for the processing of hard and brittle materials...The giant magnetostrictive rotary ultrasonic processing system(GMUPS)with a loosely-coupled contactless power transfer(LCCPT)has emerged as a high-performance technique for the processing of hard and brittle materials,owing to its high power density.A capacitive compensation is required to achieve the highest energy efficiency of GMUPS to provide sufficient vibration amplitude when it works in the resonance state.In this study,an accurate model of the optimal compensation capacitance is derived from a new electromechanical equivalent circuit model of the GMUPS with LCCPT,which consists of an equivalent mechanical circuit and an electrical circuit.The phase lag angle between the mechanical and electrical circuits is established,taking into account the non-negligible loss in energy conversion of giant magnetostrictive material at ultrasonic frequency.The change of system impedance characteristics and the effectiveness of the system compensation method under load are analyzed.Both idle vibration experiments and machining tests are conducted to verify the developed model.The results show that the GMUPS with optimal compensation capacitance can achieve the maximum idle vibration amplitude and smallest cutting force.In addition,the effects of magnetic conductive material and driving voltages on the phase lag angle are also evaluated.展开更多
基金grants from the National Natural Science Foundation of China, No. 30370533, 30570671the Educational Department Science Research Foundation of Jiangsu Province, No. 99KJB310005,05KJB310134
文摘AIM： To investigate the effects of electrical stimulation of hypothalamic paraventricular nuclei （PVN） on gastric mucosal cellular apoptosis and proliferation induced by gastric ischemia/reperfusion （I/R） injury. METHODS： For different experimental purposes, stimulating electrode plantation or electrolytic destruction of the PVN was applied, then the animals＇ GI/R injury model was established by clamping the celiac artery for 30 min and allowing reperfusing the artery for 30 rain, 1 h, 3 h or 6 h respectively. Then histological, immunohistochemistry methods were used to assess the gastric mucosal damage index, the gastric mucosal cellular apoptosis and proliferation at different times. RESULTS： The electrical stimulation of PVN significantly attenuated the GI/R injury at 30 min, i h and 3 h after reperfusion. The electrical stimulation of PVN decreased gastric mucosal apoptosis and increased gastric mucosal proliferation. The electrolytic destruction of the PVN could eliminate the protective effects of electrical stimulation of PVN on GI/R injury. These results indicated that the PVN participated in the regulation of GI/R injury as a specific area in the brain, exerting protective effects against the GI/R injury, and the protection was associated with the inhibition of cellular apoptosis and the promotion of gastric mucosal proliferation. CONCLUSION： Stimulating PVN significantly inhibits the gastric mucosal cellular apoptosis and promots gastric mucosal cellular proliferation. This may explain the protective mechanisms of electrical stimulation of PVN against GI/R injury.
文摘Textures of high-strength and low-expansion Fe-Ni alloy wires during cold-drawing processes were investigated using X-ray diffraction （XRD） and electron back scatter diffraction （EBSD） techniques. The experimental results show that the 〈111〉 and 〈100〉 fibre textures are the main texture components, and crystalline grains in the surface are more fine and uniform than those in the center of Fe-Ni alloy wires during cold-drawing processes. It is found that the volume fraction of the 〈111〉 fibre texture component determined by quantitative regression calculation of the Gaussian distribution function reaches more than 60% and the strong 〈111〉 fibre texture component favors the torsional property of Fe-Ni alloy wires.
基金Supported by National Natural Science Foundation of China, No.30570671the Educational Department Science Research Foundation of Jiangsu Province, No. 99KJB310005 and 05KJB310134
文摘AIM: To investigate the protective effect and mechanisms of ghrelin postconditioning against hypoxia/reoxygenation (H/R)-induced injury in human gastric epithelial cells. METHODS: The model of H/R injury was established in gastric epithelial cell line (GES-1) human gastric epithelial cells. Cells were divided into seven groups: normal control group (N); H/R postconditioning group; DMSO postconditioning group (DM); ghrelin postconditioning group (GH); D-Lys3-GHRP-6 + ghrelin postconditioning group (D + GH); capsazepine + ghrelin postconditioning group (C + GH); and LY294002 + ghrelin postconditioning group (L + GH). 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to detect GES-1 cell viability. Hoechst 33258 fluorochrome staining and flow cytometry were conducted to determine apoptosis of GES-1cells. Spectrophotometry was performed to determine release of lactate dehydrogenate (LDH). Protein expression of Bcl-2, Bax, Akt, and glycogen synthase kinase (GSK)-3β was determined by western blotting. Expression of vanilloid receptor subtype 1 (VR1), Akt and GSK-3β was observed by immunocytochemistry. RESULTS: Compared with the H/R group, cell viability of the GH group was significantly increased in a dosedependent manner (55.9% ± 10.0% vs 69.6% ± 9.6%, 71.9% ± 17.4%, and 76.3% ± 13.3%). Compared with the H/R group, the percentage of apoptotic cells in the GH group significantly decreased (12.38% ± 1.51% vs 6.88% ± 0.87%). Compared with the GH group, the percentage of apoptotic cells in the D + GH group, C + GH group and L + GH groups significantly increased (11.70% ± 0.88%, 11.93% ± 0.96%, 10.20% ± 1.05% vs 6.88% ± 0.87%). There were no significant differences in the percentage of apoptotic cells between the H/R and DM groups (12.38% ± 1.51% vs 13.00% ± 1.13%). There was a significant decrease in LDH release following ghrelin postconditioning compared with the H/R group (561.58 ± 64.01 U/L vs 1062.45 ± 105.29 U/L). There was a significant increase in LDH release in the D + GH, C + GH and L + GH groups compared with the GH group (816.89 ± 94.87 U/L, 870.95 ± 64.06 U/L, 838.62 ± 118.45 U/L vs 561.58 ± 64.01 U/L). There were no significant differences in LDH release between the H/R and DM groups (1062.45 ± 105.29 U/L vs 1017.65 ± 68.90 U/L). Compared with the H/R group, expression of Bcl-2 and Akt increased in the GH group, whereas expression of Bax and GSK3β decreased. Compared with the GH group, expression of Bcl-2 decreased and Bax increased in the D + GH, C + GH and L + GH groups, and Akt decreased and GSK-3β increased in the L + GH group. The H/R group also upregulated expression of VR1 and GSK-3β and downregulated Akt. The number of VR1-positive and Akt-positive cells in the GH group significantly increased, whereas the number of GSK-3β-positive cells significantly decreased. These effects of ghrelin were reversed by capsazepine and LY294002.CONCLUSION: Ghrelin postconditioning protected against H/R-induced injury in human gastric epithelial cells, which indicated that this protection might be associated with GHS-R, VR1 and the PI3K/Akt signaling pathway.
基金supported by the Guizhou provincial NaturalScience Foundation (Nos. 08097 and 2010080)the National Natural Science Foundation of China(NSFC, Grant Nos. 10778702 and 10803005)the National Basic Research Program of China(973 project 2009CB824800)
文摘This paper presents a hadronic dominated jet model to investigate multi- wavelength emission from the microquasar LS I ＋61 303. In this scenario, we take into account evolutions of the primary particles and secondary e± pairs; these pairs are produced by the collisional interactions of the accelerated protons with the cold jet protons and the stellar wind ions. In this model, the non-thermal photons are produced by πο decay emission, synchrotron and inverse Compton scattering processes from the primary electrons and secondary pairs, and relativistic bremsstrahlung emission from the secondary leptonic pairs. Based on this model framework, we show that the spectral energy distributions can be produced by the primary and secondary particles via interactions with the cold matter, and magnetic and stellar radiation fields. We also consider the attenuation of angular dependence γ-γ due to the effects of the stel- lar target photon fields. The resulting model can approximately reproduce the recent quasi-simultaneous observational data points and the non-simultaneous multi-band observations.
基金Supported by the National Natural Science Foundation of China
文摘We study non-thermal emissions from cascade processes in accreting X-raybinary pulsars. In the framework of the magnetospheric gap model, we consider threephoton fields, which are respectively from the polar cap of a pulsar, its surroundingaccretion disk and a massive companion star with a circumstellar disk, to shield thegap. The gap-accelerated ultra-relativistic electrons emit high-energy photons via cur-vature radiation and an inverse Compton scattering process, in which part of thesehigh-energy photons absorbed by interactions with the surrounding photon fields canfacilitate the following electromagnetic cascades. We first carry out numerical calcula-tions of the cascade processes in order to obtain the predicted emission spectra. As anexample, we subsequently apply this model to reproduce observations of LS I ＋61~303. We find that the results can fit observations ranging from hard X-ray to γ-raybands, In particular, they can explain the spectral cutoff feature at a few GeV. Finally,we suggest that the emissions detected by the Fermi Large Area Telescope from X-raybinary pulsars originate in the magnetosphere region of the pulsar.
基金supported by the National Natural Science Foundation of China under Grant No.62076162the Shanghai Municipal Science and Technology Major Project under Grant Nos.2021SHZDZX0102 and 20511100300.
文摘Image matting is to estimate the opacity of foreground objects from an image. A few deep learning based methods have been proposed for image matting and perform well in capturing spatially close information. However, these methods fail to capture global contextual information, which has been proved essential in improving matting performance. This is because a matting image may be up to several megapixels, which is too big for a learning-based network to capture global contextual information due to the limit size of a receptive field. Although uniformly downsampling the matting image can alleviate this problem, it may result in the degradation of matting performance. To solve this problem, we introduce a natural image matting with the attended global context method to extract global contextual information from the whole image, and to condense them into a suitable size for learning-based network. Specifically, we first leverage a deformable sampling layer to obtain condensed foreground and background attended images respectively. Then, we utilize a contextual attention layer to extract information related to unknown regions from condensed foreground and background images generated by a deformable sampling layer. Besides, our network predicts a background as well as the alpha matte to obtain more purified foreground, which contributes to better qualitative performance in composition. Comprehensive experiments show that our method achieves competitive performance on both Composition-1k and the alphamatting.com benchmark quantitatively and qualitatively.
基金financially supported by Beijing Natural Science Foundation (No. 2142037)
文摘Mn-Cu alloys could exhibit high damping ability and excellent mechanical properties after proper heat treatment. In order to reduce the influence of impurity elements on damping capacity of Mn-Cu alloys, rare ele- ment cerium （Ce） was added into MnCuNiFe alloys. It is indicated that the contents of C, S and Si which have adverse effects on the damping capacity decrease and the grains are refined with the Ce content increasing. The microstructure of the MnCuNiFeCe alloy was investigated by X-ray diffraction （XRD）, scanning electron microscope （SEM） and transmission electron microscopy （TEM）. The damping ability （tane） of the alloy was characterized by dynamical mechanical analyzer （DMA）. It is found that the damping ability （tane） retains a very high level which is all above 0.05 from the temperature of -50 to 75 ℃ with the addition of Ce element. It is expected that the Ce alloying MnCuNiFe alloy with refined grains could find wide applications in the field of industry.
基金supported by the National Natural Science Foundation of China(Nos.51875311 and 52105458)the Tsinghua-Foshan Innovation Special Fund(No.2021THFS0204)the Huaneng Group Science and Technology Research Project(No.HNKJ22-U22YYJC08),China。
文摘The giant magnetostrictive rotary ultrasonic processing system(GMUPS)with a loosely-coupled contactless power transfer(LCCPT)has emerged as a high-performance technique for the processing of hard and brittle materials,owing to its high power density.A capacitive compensation is required to achieve the highest energy efficiency of GMUPS to provide sufficient vibration amplitude when it works in the resonance state.In this study,an accurate model of the optimal compensation capacitance is derived from a new electromechanical equivalent circuit model of the GMUPS with LCCPT,which consists of an equivalent mechanical circuit and an electrical circuit.The phase lag angle between the mechanical and electrical circuits is established,taking into account the non-negligible loss in energy conversion of giant magnetostrictive material at ultrasonic frequency.The change of system impedance characteristics and the effectiveness of the system compensation method under load are analyzed.Both idle vibration experiments and machining tests are conducted to verify the developed model.The results show that the GMUPS with optimal compensation capacitance can achieve the maximum idle vibration amplitude and smallest cutting force.In addition,the effects of magnetic conductive material and driving voltages on the phase lag angle are also evaluated.
