Artificial guidance channels containing Schwann cells can promote the regeneration of injured peripheral nerve over long distances. However, primary Schwann cells are not suitable for autotransplantation. Under specif...Artificial guidance channels containing Schwann cells can promote the regeneration of injured peripheral nerve over long distances. However, primary Schwann cells are not suitable for autotransplantation. Under specific conditions, skin-derived progenitors can be induced to dif- ferentiate into Schwann cells. Therefore, adult rat dorsal skin （dermis）-derived progenitors were isolated and induced to differentiate with DMEM/F12 containing B27, neuregulin 1, and for- skolin. Immunofluorescence staining and reverse transcription polymerase chain reaction （RT- PCR） confirmed that the resultant cells were indeed Schwann cells. Artificial guidance channels containing skin-derived progenitors, Schwann cells originating from skin-derived progenitors, or primary Schwann cells were used to bridge 5 mm sciatic nerve defects. Schwann cells originating from skin-derived progenitors significantly promoted sciatic nerve axonal regeneration. The sig- nificant recovery of injured rat sciatic nerve function after the transplantation of Schwann cells originating from skin-derived progenitors was confirmed by electromyogram. The therapeutic effect of Schwann cells originating from skin-derived progenitors was better than that of skin-de- rived progenitors. These findings indicate that Schwann cells originating from skin-derived precursors can promote peripheral nerve regeneration in rats.展开更多
During spaceflight,the cardiovascular system undergoes remarkable adaptation to microgravity and faces the risk of cardiac remodeling.Therefore,the effects and mechanisms of microgravity on cardiac morphology,physiolo...During spaceflight,the cardiovascular system undergoes remarkable adaptation to microgravity and faces the risk of cardiac remodeling.Therefore,the effects and mechanisms of microgravity on cardiac morphology,physiology,metabolism,and cellular biology need to be further investigated.Since China started constructing the China Space Station(CSS)in 2021,we have taken advantage of the Shenzhou-13 capsule to send human pluripotent stem cell-derived cardiomyocytes(hPSC-CMs)to the Tianhe core module of the CSS.In this study,hPSC-CMs subjected to space microgravity showed decreased beating rate and abnormal intracellular calcium cycling.Metabolomic and transcriptomic analyses revealed a battery of metabolic remodeling of hPSC-CMs in spaceflight,especially thiamine metabolism.The microgravity condition blocked the thiamine intake in hPSC-CMs.The decline of thiamine utilization under microgravity or by its antagonistic analog amprolium affected the process of the tricarboxylic acid cycle.It decreased ATP production,which led to cytoskeletal remodeling and calcium homeostasis imbalance in hPSC-CMs.More importantly,in vitro and in vivo studies suggest that thiamine supplementation could reverse the adaptive changes induced by simulated microgravity.This study represents the first astrobiological study on the China Space Station and lays a solid foundation for further aerospace biomedical research.These data indicate that intervention of thiamine-modified metabolic reprogramming in human cardiomyocytes during spaceflight might be a feasible countermeasure against microgravity.展开更多
Despite laboratory experiments that have been performed to study internal heavy metal release,our understanding of how heavy metals release in shallow eutrophic lakes remains limited for lacking in-situ evidence.This ...Despite laboratory experiments that have been performed to study internal heavy metal release,our understanding of how heavy metals release in shallow eutrophic lakes remains limited for lacking in-situ evidence.This study used automatic environmental sensors and a water sampling system to conduct high-frequency in-situ observations(1-hr intervals)of water environmental variables and to collect water samples(3-hr intervals),with which to examine the release of internal heavy metals in Lake Taihu,China.Under conditions of disturbance by strong northerly winds,sediment resuspension in both the estuary area and the lake center caused particulate heavy metal resuspension.However,the patterns of concentrations of dissolved heavy metals in these two areas were complex.The concentrations of dissolved Se and Mo increased in both areas,indicating that release of internal dissolved Se and Mo is triggered by sediment resuspension.The concentrations of dissolved Ni,Zn,As,Mn,Cu,V,and Co tended to increase in the estuary area but decrease in the lake center.The different trends between these two areas were controlled by pH and cyanobacteria,which are related to eutrophication.During the strong northerly winds,the decrease in concentrations of dissolved heavy metals in the lake center was attributable primarily to absorption by the increased suspended solids,and to growth-related assimilation or surface adsorption by the increased cyanobacteria.The findings of this study suggest that,short-term changes of environmental conditions are very important in relation to reliable monitoring and risk assessment of heavy metals in shallow eutrophic lakes.展开更多
基金supported by the National Natural Science Foundation of China,No.81171194
文摘Artificial guidance channels containing Schwann cells can promote the regeneration of injured peripheral nerve over long distances. However, primary Schwann cells are not suitable for autotransplantation. Under specific conditions, skin-derived progenitors can be induced to dif- ferentiate into Schwann cells. Therefore, adult rat dorsal skin （dermis）-derived progenitors were isolated and induced to differentiate with DMEM/F12 containing B27, neuregulin 1, and for- skolin. Immunofluorescence staining and reverse transcription polymerase chain reaction （RT- PCR） confirmed that the resultant cells were indeed Schwann cells. Artificial guidance channels containing skin-derived progenitors, Schwann cells originating from skin-derived progenitors, or primary Schwann cells were used to bridge 5 mm sciatic nerve defects. Schwann cells originating from skin-derived progenitors significantly promoted sciatic nerve axonal regeneration. The sig- nificant recovery of injured rat sciatic nerve function after the transplantation of Schwann cells originating from skin-derived progenitors was confirmed by electromyogram. The therapeutic effect of Schwann cells originating from skin-derived progenitors was better than that of skin-de- rived progenitors. These findings indicate that Schwann cells originating from skin-derived precursors can promote peripheral nerve regeneration in rats.
基金This study was supported by the National Key R&D Program of China(2022YFA1104300)the Space Medical Experiment Project of China Manned Space Program(HYZHXM01018)+4 种基金the National Natural Science Foundation of China(82241202)Scientific Innovation Project of the State Key Laboratory of Radiation Medicine and Protection(GZC00101)The Natural Science Foundation of the Jiangsu Higher Education Institutions of China(21KJB310003)Jiangsu Cardiovascular Medicine Innovation Center(CXZX202210)National Center for International Research(2017B01012).
文摘During spaceflight,the cardiovascular system undergoes remarkable adaptation to microgravity and faces the risk of cardiac remodeling.Therefore,the effects and mechanisms of microgravity on cardiac morphology,physiology,metabolism,and cellular biology need to be further investigated.Since China started constructing the China Space Station(CSS)in 2021,we have taken advantage of the Shenzhou-13 capsule to send human pluripotent stem cell-derived cardiomyocytes(hPSC-CMs)to the Tianhe core module of the CSS.In this study,hPSC-CMs subjected to space microgravity showed decreased beating rate and abnormal intracellular calcium cycling.Metabolomic and transcriptomic analyses revealed a battery of metabolic remodeling of hPSC-CMs in spaceflight,especially thiamine metabolism.The microgravity condition blocked the thiamine intake in hPSC-CMs.The decline of thiamine utilization under microgravity or by its antagonistic analog amprolium affected the process of the tricarboxylic acid cycle.It decreased ATP production,which led to cytoskeletal remodeling and calcium homeostasis imbalance in hPSC-CMs.More importantly,in vitro and in vivo studies suggest that thiamine supplementation could reverse the adaptive changes induced by simulated microgravity.This study represents the first astrobiological study on the China Space Station and lays a solid foundation for further aerospace biomedical research.These data indicate that intervention of thiamine-modified metabolic reprogramming in human cardiomyocytes during spaceflight might be a feasible countermeasure against microgravity.
基金supported by the National Key R&D Program of China(No.2017YFC0405205)the National Natural Science Foundation of China(Nos.41971047,41621002,41661134036,41301531)+1 种基金the Key Research Program of Frontier Sciences,Chinese Academy of Sciences(No.QYZDJ-SSW-DQC008)the“One-Three-Five”Strategic Planning of the Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences(No.NIGLAS2017GH04)。
文摘Despite laboratory experiments that have been performed to study internal heavy metal release,our understanding of how heavy metals release in shallow eutrophic lakes remains limited for lacking in-situ evidence.This study used automatic environmental sensors and a water sampling system to conduct high-frequency in-situ observations(1-hr intervals)of water environmental variables and to collect water samples(3-hr intervals),with which to examine the release of internal heavy metals in Lake Taihu,China.Under conditions of disturbance by strong northerly winds,sediment resuspension in both the estuary area and the lake center caused particulate heavy metal resuspension.However,the patterns of concentrations of dissolved heavy metals in these two areas were complex.The concentrations of dissolved Se and Mo increased in both areas,indicating that release of internal dissolved Se and Mo is triggered by sediment resuspension.The concentrations of dissolved Ni,Zn,As,Mn,Cu,V,and Co tended to increase in the estuary area but decrease in the lake center.The different trends between these two areas were controlled by pH and cyanobacteria,which are related to eutrophication.During the strong northerly winds,the decrease in concentrations of dissolved heavy metals in the lake center was attributable primarily to absorption by the increased suspended solids,and to growth-related assimilation or surface adsorption by the increased cyanobacteria.The findings of this study suggest that,short-term changes of environmental conditions are very important in relation to reliable monitoring and risk assessment of heavy metals in shallow eutrophic lakes.
