Geochemistry, zircon U–Pb geochronology, and Hf isotope data for the Early Paleozoic granites in the Baoshan Block reveal the Early Paleozoic tectonic evolution of the Proto-Tethys. The samples are high-K, calcalkali...Geochemistry, zircon U–Pb geochronology, and Hf isotope data for the Early Paleozoic granites in the Baoshan Block reveal the Early Paleozoic tectonic evolution of the Proto-Tethys. The samples are high-K, calcalkaline, strongly peraluminous rocks with A/CNK values of 1.37–1.46, are enriched in SiO2, K2O, and Rb, and are depleted in Nb, P, Ti, Eu, and heavy rare earth elements,which indicates the crystallization fractionation of the granitic magma. Zircon U–Pb dating indicates that they formed in ca. 480 Ma. The Nansa granites have εHf(t) values ranging from-16.04 to 4.36 with corresponding TC DMages of 2.10–0.81 Ga, which suggests the magmas derived from the partial melting of ancient metasedimentary with minor involvement of mantle-derived components. A synthesis of data for the Early Paleozoic igneous rocks in the Baoshan block and adjacent(Tengchong,Qiangtang, Sibumasu, Himalaya, etc.) blocks indicates that these blocks were all aligned along the proto-Tethyan margin of East Gondwana in the Early Paleozoic. The Early Paleozoic S-type granites from Nansa were generated in a high-temperature and low-pressure(HTLP) extensional tectonic setting, which resulted from Andean-type orogeny instead of the final assembly of Gondwana or crustal extension in a non-arc environment. In certain places, an expanding environment may exist in opposition to the tectonic backdrop of the lithosphere’s thickening and shortening, leading the crust to melt and decompress,mantle-derived materials to mix, and a small quantity of peraluminous granite to emerge.展开更多
Railway accidents,particularly serious derailments,can lead to catastrophic consequences.Therefore,it is essential to prevent derailment escalation to reduce the likelihood of severe derailments.Train post-derailment ...Railway accidents,particularly serious derailments,can lead to catastrophic consequences.Therefore,it is essential to prevent derailment escalation to reduce the likelihood of severe derailments.Train post-derailment behaviours and containment methods play a critical role in preventing derailment escalation and providing passive safety protection and accident prevention in the event of a derailment.However,despite the increasing attention on this field from academia and industry in recent years,there is a lack of systematic exploration and summarization of emerging applications and containment methods in train post-derailment research.For this reason,this paper presents a comprehensive review of existing studies on train post-derailment behaviours,encompassing various topics such as post-derailment contact-impact models,dynamic modelling and simulation techniques,and the primary factors influencing post-derailment behaviours.Significantly,this review introduces and elucidates substitute guidance mechanisms(SGMs),which serve as railway-specific passive safety protection and accident prevention measures.The various types of SGMs are depicted,and their ongoing developments and applications are explored in depth.The review additionally points out several unresolved challenges including the adverse effects of SGMs,and proposes future research directions to advance the theoretical understanding and practical application of train post-derailment behaviours and containment methods.This review seeks to be a valuable reference for railway industry professionals in preventing catastrophic derailment consequences through post-derailment containment methods.展开更多
Establishing the Greater Food Approach and promoting the Yangtze River Economic Belt s national major regional development strategy can better support and serve the agricultural power and Chinese-style modernization.T...Establishing the Greater Food Approach and promoting the Yangtze River Economic Belt s national major regional development strategy can better support and serve the agricultural power and Chinese-style modernization.This paper introduces the characteristics of fruit industry in 16 autonomous prefectures and 47 autonomous counties under the jurisdiction of the Yangtze River Economic Belt.It studies the intellectual property resources of brand marks from the aspects of geographical indications,collective trademarks,certification trademarks,well-known trademarks in China and national design patents,and analyzes the main problems of brand and high-quality development of fruit industry in these ethnic autonomous areas.Finally,it puts forward some strategies,such as improving the protection of intellectual property rights of geographical indications,using intellectual property rights of brand signs,building modern seed industry upgrading project,drawing lessons from the experience of thousand villages demonstration project,ensuring that large-scale poverty does not occur,and building a diversified food supply system.展开更多
Accelerating the construction of agricultural power,further promoting the high-quality development of the Yangtze River Economic Belt,and better supporting and serving Chinese-style modernization are the key issues at...Accelerating the construction of agricultural power,further promoting the high-quality development of the Yangtze River Economic Belt,and better supporting and serving Chinese-style modernization are the key issues at present.This paper first introduces the Yangtze River Economic Belt and its ethnic areas,and studies the characteristics of the grain and oil industry in the ethnic autonomous areas of the economic belt,as well as the current situation of various types of intellectual property resources,such as industrial cultural heritage,scientific and tech-nological innovation,and brand marking intellectual property.Besides,it analyzes the main problems in the high-quality development of grain and oil in the Yangtze River Economic Belt ethnic areas.Finally,it comes up with recommendations,including protecting the intellectual prop-erty rights of cultural heritage in the field of grain and oil,leading the inheritance and development of excellent traditional Chinese culture,use scientific and technological innovation intellectual property rights to promote the innovation-driven development strategy of the grain and oil in-dustry,and providing counterpart assistance to ethnic autonomous areas in the Yangtze River Economic Belt to further promote the high-quality development of the Yangtze River Economic Belt.展开更多
Only by improving the production capacity of domestic herbal edible oil can China ensure the safety of the supply chain of the important industrial chain of vegetable edible oil in China and practice the big food conc...Only by improving the production capacity of domestic herbal edible oil can China ensure the safety of the supply chain of the important industrial chain of vegetable edible oil in China and practice the big food concept.In addition to rice,maize,and soybean for using as grain and oil,there are eight kinds of herbaceous edible oil crops in Hubei Province,including rape,peanut,sesame,sunflower,perilla(perilla seed),cotton,linen and tiger nut(Cyperus esculentus).This paper studies the main industries of herbaceous edible oil crops and their intellectual property resources in Hubei Province,and analyzes the main problems of its inheritance,innovation and high-quality development under the strategy of strengthening the country with intellectual property.Finally,it proposes the countermeasures of carrying forward and inheriting traditional knowledge and traditional culture,maintaining the biodiversity of crops,strengthening the creation of new plant varieties and breeding patents,and opening up the whole chain of intellectual property rights.展开更多
Only by developing woody edible oilseeds industry can we ensure the safety of the important industrial chain and supply chain of vegetable edible oil in China.There are 13 kinds of woody edible oil plants in Hubei,inc...Only by developing woody edible oilseeds industry can we ensure the safety of the important industrial chain and supply chain of vegetable edible oil in China.There are 13 kinds of woody edible oil plants in Hubei,including Camellia oleifera Abel(oil tea),Juglans regia L.(walnut),Olea europaea L.(olive)and Paeonia suffruticosa(oil peony).This paper studies the main industries of woody edible oilseeds in Hubei Province and their intellectual property resources,and analyzes the main problems in their intellectual property protection,inheritance,innovation and development.Finally,it proposes some strategies,including carrying forward the traditional knowledge related to woody edible oilseeds,innovating the"agricultural chip",creating key counties of national woody edible oil seeds,and developing industrial clusters with national advantages and characteristics.展开更多
Aiming at the problem that the existing ankle rehabilitation robot is difficult to fully fit the complex motion of human ankle joint and has poor human-machine motion compatibility,an equivalent series mechanism model...Aiming at the problem that the existing ankle rehabilitation robot is difficult to fully fit the complex motion of human ankle joint and has poor human-machine motion compatibility,an equivalent series mechanism model that is highly matched with the actual bone structure of the human ankle joint is proposed and mapped into a parallel rehabilita-tion mechanism.The parallel rehabilitation mechanism has two virtual motion centers(VMCs),which can simulate the complex motion of the ankle joint,adapt to the individual differences of various patients,and can meet the reha-bilitation needs of both left and right feet of patients.Firstly,based on the motion properties and physiological structure of the human ankle joint,the mapping relationship between the rehabilitation mechanism and ankle joint is determined,and the series equivalent model of the ankle joint is established.According to the kinematic and con-straint properties of the ankle equivalent model,the configuration design of the parallel ankle rehabilitation robot is carried out.Secondly,according to the intersecting motion planes theory,the full-cycle mobility of the mechanism is proved,and the continuous axis of the mechanism is judged based on the constraint power and its derivative.Then,the kinematics of the parallel ankle rehabilitation robot is analyzed.Finally,based on the OpenSim biomechanical soft-ware,a human-machine coupling rehabilitation simulation model is established to evaluate the rehabilitation effect,which lays the foundation for the formulation of a rehabilitation strategy for the later prototype.展开更多
Polymer electrolytes(PEs)have been long recognized as the key materials to enable energy-dense batteries and render flexible energy devices practically viable,owing to their chemical and mechanical reliability.However...Polymer electrolytes(PEs)have been long recognized as the key materials to enable energy-dense batteries and render flexible energy devices practically viable,owing to their chemical and mechanical reliability.However,much of their promise is yet to be realized.The roomtemperature ion conductivity of existing PEs still falls short of the implementation criterion of 10^(-4) S cm^(-1) on the promise of acceptable mechanical properties,thereby precluding their practical application.The twin but inversely related duties of polymers,that is,functioning as both an ion-conducting medium and a structural backbone,underlie this issue but are less elucidated systematically.The polyacrylate(PA)family is among promising polymer matrices on account of ester polarity,electrode compatibility,chemical tunability,and mechanical durability.The extensive applicability of PA in plasticized gels,dry solids,and emerging composites makes PA-based PEs representative to illustrate the trade-off between ion conduction and mechanical strength.We herein seek to outline the stated long-standing conflict exemplified by PA-based PEs,focusing on crucial strategies toward balancing and reconciling the two mutually exclusive properties,with the intention of offering designing guidelines for next-generation PEs.展开更多
Water resources are an indispensable and valuable resource for human survival and development.Water quality predicting plays an important role in the protection and development of water resources.It is difficult to pr...Water resources are an indispensable and valuable resource for human survival and development.Water quality predicting plays an important role in the protection and development of water resources.It is difficult to predictwater quality due to its random and trend changes.Therefore,amethod of predicting water quality which combines Auto Regressive Integrated Moving Average(ARIMA)and clusteringmodelwas proposed in this paper.By taking thewater qualitymonitoring data of a certain river basin as a sample,thewater quality Total Phosphorus(TP)index was selected as the prediction object.Firstly,the sample data was cleaned,stationary analyzed,and white noise analyzed.Secondly,the appropriate parameters were selected according to the Bayesian Information Criterion(BIC)principle,and the trend component characteristics were obtained by using ARIMA to conduct water quality predicting.Thirdly,the relationship between the precipitation and the TP index in themonitoring water field was analyzed by the K-means clusteringmethod,and the random incremental characteristics of precipitation on water quality changes were calculated.Finally,by combining with the trend component characteristics and the random incremental characteristics,the water quality prediction results were calculated.Compared with the ARIMA water quality prediction method,experiments showed that the proposed method has higher accuracy,and its Mean Absolute Error(MAE),Mean Square Error(MSE),and Mean Absolute Percentage Error(MAPE)were respectively reduced by 44.6%,56.8%,and 45.8%.展开更多
At present,water pollution has become an important factor affecting and restricting national and regional economic development.Total phosphorus is one of the main sources of water pollution and eutrophication,so the p...At present,water pollution has become an important factor affecting and restricting national and regional economic development.Total phosphorus is one of the main sources of water pollution and eutrophication,so the prediction of total phosphorus in water quality has good research significance.This paper selects the total phosphorus and turbidity data for analysis by crawling the data of the water quality monitoring platform.By constructing the attribute object mapping relationship,the correlation between the two indicators was analyzed and used to predict the future data.Firstly,the monthly mean and daily mean concentrations of total phosphorus and turbidity outliers were calculated after cleaning,and the correlation between them was analyzed.Secondly,the correlation coefficients of different times and frequencies were used to predict the values for the next five days,and the data trend was predicted by python visualization.Finally,the real value was compared with the predicted value data,and the results showed that the correlation between total phosphorus and turbidity was useful in predicting the water quality.展开更多
Objective:To study the mechanism of lncRNA p21 inhibiting the growth and metastasis of human gastric cancer SGC7901/GES-1 cells by mediating the Wnt/β-catenin signaling pathway.Methods:Lentiviral overexpression of ln...Objective:To study the mechanism of lncRNA p21 inhibiting the growth and metastasis of human gastric cancer SGC7901/GES-1 cells by mediating the Wnt/β-catenin signaling pathway.Methods:Lentiviral overexpression of lncRNA-p21 in human gastric cancer SGC7901/GES-1 cell transfections was observed and analyzed for in vitro migration,invasion,cell morphology and proliferation.Besides.Wnt/β-catenin signaling pathway was tested for direct involvement in lncRNA-p21-mediated inhibition of gastric cancer cell growth and proliferation.Wnt/β-catenin signaling pathway was validated using Li-C1.Results:Gastric cancer SGC7901/GES-1 cells in the overexpression of lncRNA-p21 showed changes in stellate morphology,low invasion,or spindle-shaped morphology.LncRNA-p21 inhibited the growth and proliferation of gastric cancer SGC7901/GES-1 cells both in vivo and in vitro,and Wnt/β-catenin signaling pathway mediated the proliferation,invasion,and migration of lncRNA-p21 on gastric cancer SGC7901/GES-1 cells.Conclusion:LncRNA-p21 can inhibit the growth and metastasis of gastric cancer SGC7901/GES-1 cells in vitro and in vivo,and the inhibition of lncRNA p21 is mainly mediated by inhibiting the Wnt/β-catenin signaling pathway.展开更多
Objective:To elucidate the role and mechanism of cysteine protease inhibitor SN(CST1)in promoting the metastasis of gastric cancer.Methods:(1)Firstly,6 pairs of cancer and paracancer tissue samples from gastric cancer...Objective:To elucidate the role and mechanism of cysteine protease inhibitor SN(CST1)in promoting the metastasis of gastric cancer.Methods:(1)Firstly,6 pairs of cancer and paracancer tissue samples from gastric cancer patients without distant metastasis and 5 pairs of cancer and paracancer tissue samples from gastric cancer patients with peritoneal metastasis were collected for transcriptome sequencing.Statistical analysis was performed on the sequencing results to identify significantly upregulated differential genes.(2)Another 75 pairs of cancer and paracancer tissue samples were collected from gastric cancer patients,and the protein and total RNA of gastric cancer tissue samples were extracted.Western blot and quantitative reverse transcription polymerase chain reaction(qRT-PCR)were used to detect the expression of CST1 protein and messenger RNA(mRNA)in gastric cancer and adjacent tissues.(3)The total protein and total RNA of AGS,BGC823,HGC-27,MGC803,MKN45,SGC7901,and SNU-1 gastric cancer cells and normal gastric mucosal epithelial cells GES-1 were extracted.Western blot and qRT-PCR were used to detect CST1 protein and mRNA expression.Results:(1)The significantly upregulated differential gene CST1 was screened,and the expression of CST1 in gastric cancer tissues was significantly higher than that in adjacent tissues.(2)Compared with normal gastric mucosal epithelial cells GES-1,the expression of CST1 in gastric cancer cell lines was upregulated,and the expression of CST1 in HGC-27 and SNU-1 was relatively low,while the expression of CST1 in AGS and MKN45 was relatively high.At the same time,a stable cell line of HGC-27 overexpressing CST1 and MKN45 knocking down CST1 was successfully constructed.(3)Overexpression or knockdown of CST1 did not significantly change the proliferation ability of gastric cancer cells,but can promote the migration and invasion of gastric cancer cells.(4)CST1 may promote the metastasis of gastric cancer cells by activating the epithelial-mesenchymal transition(EMT)signaling pathway.Conclusion:CST1 gene promotes the migration of gastric cancer cells,and we found through animal experiments that CST1 can affect the metastasis and invasion function of gastric cancer and is negatively correlated with the level of E-cadherin.展开更多
Because the inhibition of Nogo proteins can promote neurite growth and nerve cell differentiation, a cell-scaffold complex seeded with Nogo receptor(Ng R)-silenced neural stem cells and Schwann cells may be able to im...Because the inhibition of Nogo proteins can promote neurite growth and nerve cell differentiation, a cell-scaffold complex seeded with Nogo receptor(Ng R)-silenced neural stem cells and Schwann cells may be able to improve the microenvironment for spinal cord injury repair. Previous studies have found that mild hypothermia helps to attenuate secondary damage in the spinal cord and exerts a neuroprotective effect. Here, we constructed a cell-scaffold complex consisting of a poly(D,L-lactide-co-glycolic acid)(PLGA) scaffold seeded with Ng R-silenced neural stem cells and Schwann cells, and determined the effects of mild hypothermia combined with the cell-scaffold complexes on the spinal cord hemi-transection injury in the T9 segment in rats. Compared with the PLGA group and the Ng R-silencing cells + PLGA group, hindlimb motor function and nerve electrophysiological function were clearly improved, pathological changes in the injured spinal cord were attenuated, and the number of surviving cells and nerve fibers were increased in the group treated with the Ng R-silenced cell scaffold + mild hypothermia at 34°C for 6 hours. Furthermore, fewer pathological changes to the injured spinal cord and more surviving cells and nerve fibers were found after mild hypothermia therapy than in injuries not treated with mild hypothermia. These experimental results indicate that mild hypothermia combined with Ng R gene-silenced cells in a PLGA scaffold may be an effective therapy for treating spinal cord injury.展开更多
Inhibition of neurite growth, which is mediated by the Nogo-66 receptor (NgR), affects nerve regeneration following neural stem cell (NSC) transplantation. The present study utilized RNA interference to silence NgR ge...Inhibition of neurite growth, which is mediated by the Nogo-66 receptor (NgR), affects nerve regeneration following neural stem cell (NSC) transplantation. The present study utilized RNA interference to silence NgR gene expression in NSCs, which were subsequently transplanted into rats with traumatic brain injury. Following transplantation of NSCs transfected with small interfering RNA, typical neural cell-like morphology was detected in injured brain tissues, and was accompanied by absence of brain tissue cavity, increased growth-associated protein 43 mRNA and protein expression, and improved neurological function compared with NSC transplantation alone. Results demonstrated that NSC transplantation with silenced NgR gene promoted functional recovery following brain injury.展开更多
Inhibition of neurite growth,which is in large part mediated by the Nogo-66 receptor,affects neural regeneration following bone marrow mesenchymal stem cell transplantation.The tissue engineering scaffold poly(D,L-lac...Inhibition of neurite growth,which is in large part mediated by the Nogo-66 receptor,affects neural regeneration following bone marrow mesenchymal stem cell transplantation.The tissue engineering scaffold poly(D,L-lactide-co-glycolic acid) has good histocompatibility and can promote the growth of regenerating nerve fibers.The present study used small interfering RNA to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells and Schwann cells,which were subsequently transplanted with poly(D,L-lactide-co-glycolic acid) into the spinal cord lesion regions in rats.Simultaneously,rats treated with scaffold only were taken as the control group.Hematoxylin-eosin staining and immunohistochemistry revealed that at 4 weeks after transplantation,rats had good motor function of the hind limb after treatment with Nogo-66 receptor gene-silenced cells plus the poly(D,L-lactide-co-glycolic acid) scaffold compared with rats treated with scaffold only,and the number of bone marrow mesenchymal stem cells and neuron-like cells was also increased.At 8 weeks after transplantation,horseradish peroxidase tracing and transmission electron microscopy showed a large number of unmyelinated and myelinated nerve fibers,as well as intact regenerating axonal myelin sheath following spinal cord hemisection injury.These experimental findings indicate that transplantation of Nogo-66 receptor gene-silenced bone marrow mesenchymal stem cells and Schwann cells plus a poly(D,L-lactide-co-glycolic acid) scaffold can significantly enhance axonal regeneration of spinal cord neurons and improve motor function of the extremities in rats following spinal cord injury.展开更多
基金funded by the National Natural Science Foundation of China (2019M653840XB)the National Natural Science Foundation of China (41972043 and 42062006)。
文摘Geochemistry, zircon U–Pb geochronology, and Hf isotope data for the Early Paleozoic granites in the Baoshan Block reveal the Early Paleozoic tectonic evolution of the Proto-Tethys. The samples are high-K, calcalkaline, strongly peraluminous rocks with A/CNK values of 1.37–1.46, are enriched in SiO2, K2O, and Rb, and are depleted in Nb, P, Ti, Eu, and heavy rare earth elements,which indicates the crystallization fractionation of the granitic magma. Zircon U–Pb dating indicates that they formed in ca. 480 Ma. The Nansa granites have εHf(t) values ranging from-16.04 to 4.36 with corresponding TC DMages of 2.10–0.81 Ga, which suggests the magmas derived from the partial melting of ancient metasedimentary with minor involvement of mantle-derived components. A synthesis of data for the Early Paleozoic igneous rocks in the Baoshan block and adjacent(Tengchong,Qiangtang, Sibumasu, Himalaya, etc.) blocks indicates that these blocks were all aligned along the proto-Tethyan margin of East Gondwana in the Early Paleozoic. The Early Paleozoic S-type granites from Nansa were generated in a high-temperature and low-pressure(HTLP) extensional tectonic setting, which resulted from Andean-type orogeny instead of the final assembly of Gondwana or crustal extension in a non-arc environment. In certain places, an expanding environment may exist in opposition to the tectonic backdrop of the lithosphere’s thickening and shortening, leading the crust to melt and decompress,mantle-derived materials to mix, and a small quantity of peraluminous granite to emerge.
基金support from the National Natural Science Foundation of China (No.52172407 and No.U19A20110)the Natural Science Foundation of Sichuan Province (No.2022NSFSC0415).
文摘Railway accidents,particularly serious derailments,can lead to catastrophic consequences.Therefore,it is essential to prevent derailment escalation to reduce the likelihood of severe derailments.Train post-derailment behaviours and containment methods play a critical role in preventing derailment escalation and providing passive safety protection and accident prevention in the event of a derailment.However,despite the increasing attention on this field from academia and industry in recent years,there is a lack of systematic exploration and summarization of emerging applications and containment methods in train post-derailment research.For this reason,this paper presents a comprehensive review of existing studies on train post-derailment behaviours,encompassing various topics such as post-derailment contact-impact models,dynamic modelling and simulation techniques,and the primary factors influencing post-derailment behaviours.Significantly,this review introduces and elucidates substitute guidance mechanisms(SGMs),which serve as railway-specific passive safety protection and accident prevention measures.The various types of SGMs are depicted,and their ongoing developments and applications are explored in depth.The review additionally points out several unresolved challenges including the adverse effects of SGMs,and proposes future research directions to advance the theoretical understanding and practical application of train post-derailment behaviours and containment methods.This review seeks to be a valuable reference for railway industry professionals in preventing catastrophic derailment consequences through post-derailment containment methods.
基金Supported by Special Soft Science Research Project for Hubei Province Science and Technology Innovation Talents and Services(2022EDA060).
文摘Establishing the Greater Food Approach and promoting the Yangtze River Economic Belt s national major regional development strategy can better support and serve the agricultural power and Chinese-style modernization.This paper introduces the characteristics of fruit industry in 16 autonomous prefectures and 47 autonomous counties under the jurisdiction of the Yangtze River Economic Belt.It studies the intellectual property resources of brand marks from the aspects of geographical indications,collective trademarks,certification trademarks,well-known trademarks in China and national design patents,and analyzes the main problems of brand and high-quality development of fruit industry in these ethnic autonomous areas.Finally,it puts forward some strategies,such as improving the protection of intellectual property rights of geographical indications,using intellectual property rights of brand signs,building modern seed industry upgrading project,drawing lessons from the experience of thousand villages demonstration project,ensuring that large-scale poverty does not occur,and building a diversified food supply system.
基金Supported by Special Soft Science Research Project for Hubei Province Science and Technology Innovation Talents and Services(2022EDA060).
文摘Accelerating the construction of agricultural power,further promoting the high-quality development of the Yangtze River Economic Belt,and better supporting and serving Chinese-style modernization are the key issues at present.This paper first introduces the Yangtze River Economic Belt and its ethnic areas,and studies the characteristics of the grain and oil industry in the ethnic autonomous areas of the economic belt,as well as the current situation of various types of intellectual property resources,such as industrial cultural heritage,scientific and tech-nological innovation,and brand marking intellectual property.Besides,it analyzes the main problems in the high-quality development of grain and oil in the Yangtze River Economic Belt ethnic areas.Finally,it comes up with recommendations,including protecting the intellectual prop-erty rights of cultural heritage in the field of grain and oil,leading the inheritance and development of excellent traditional Chinese culture,use scientific and technological innovation intellectual property rights to promote the innovation-driven development strategy of the grain and oil in-dustry,and providing counterpart assistance to ethnic autonomous areas in the Yangtze River Economic Belt to further promote the high-quality development of the Yangtze River Economic Belt.
基金Supported by Special Soft Science Research Project for Hubei Province Science and Technology Innovation Talents and Services (2022EDA060).
文摘Only by improving the production capacity of domestic herbal edible oil can China ensure the safety of the supply chain of the important industrial chain of vegetable edible oil in China and practice the big food concept.In addition to rice,maize,and soybean for using as grain and oil,there are eight kinds of herbaceous edible oil crops in Hubei Province,including rape,peanut,sesame,sunflower,perilla(perilla seed),cotton,linen and tiger nut(Cyperus esculentus).This paper studies the main industries of herbaceous edible oil crops and their intellectual property resources in Hubei Province,and analyzes the main problems of its inheritance,innovation and high-quality development under the strategy of strengthening the country with intellectual property.Finally,it proposes the countermeasures of carrying forward and inheriting traditional knowledge and traditional culture,maintaining the biodiversity of crops,strengthening the creation of new plant varieties and breeding patents,and opening up the whole chain of intellectual property rights.
基金Supported by Special Soft Science Research Project for Hubei Province Science and Technology Innovation Talents and Services(2022EDA060).
文摘Only by developing woody edible oilseeds industry can we ensure the safety of the important industrial chain and supply chain of vegetable edible oil in China.There are 13 kinds of woody edible oil plants in Hubei,including Camellia oleifera Abel(oil tea),Juglans regia L.(walnut),Olea europaea L.(olive)and Paeonia suffruticosa(oil peony).This paper studies the main industries of woody edible oilseeds in Hubei Province and their intellectual property resources,and analyzes the main problems in their intellectual property protection,inheritance,innovation and development.Finally,it proposes some strategies,including carrying forward the traditional knowledge related to woody edible oilseeds,innovating the"agricultural chip",creating key counties of national woody edible oil seeds,and developing industrial clusters with national advantages and characteristics.
基金Supported by National Natural Science Foundation of China(Grant No.52075145)S&T Program of Hebei Province of China(Grant No.20281805Z)+1 种基金Hebei Provincial Natural Science Foundation of China(Grant No.E2022202130)Central Government Guides Basic Research Projects of Local Science and Technology Development Funds of China(Grant No.206Z1801G).
文摘Aiming at the problem that the existing ankle rehabilitation robot is difficult to fully fit the complex motion of human ankle joint and has poor human-machine motion compatibility,an equivalent series mechanism model that is highly matched with the actual bone structure of the human ankle joint is proposed and mapped into a parallel rehabilita-tion mechanism.The parallel rehabilitation mechanism has two virtual motion centers(VMCs),which can simulate the complex motion of the ankle joint,adapt to the individual differences of various patients,and can meet the reha-bilitation needs of both left and right feet of patients.Firstly,based on the motion properties and physiological structure of the human ankle joint,the mapping relationship between the rehabilitation mechanism and ankle joint is determined,and the series equivalent model of the ankle joint is established.According to the kinematic and con-straint properties of the ankle equivalent model,the configuration design of the parallel ankle rehabilitation robot is carried out.Secondly,according to the intersecting motion planes theory,the full-cycle mobility of the mechanism is proved,and the continuous axis of the mechanism is judged based on the constraint power and its derivative.Then,the kinematics of the parallel ankle rehabilitation robot is analyzed.Finally,based on the OpenSim biomechanical soft-ware,a human-machine coupling rehabilitation simulation model is established to evaluate the rehabilitation effect,which lays the foundation for the formulation of a rehabilitation strategy for the later prototype.
基金National Natural Science Foundation of China,Grant/Award Numbers:21975271,22139001Shandong Energy Institute,Grant/Award Number:SEI I202127+3 种基金Youth Innovation Promotion Association of CAS,Grant/Award Number:2019214Key Scientific and Technological Innovation Project of Shandong,Grant/Award Number:2020CXGC010401Major basic research projects of Shandong Natural Science Foundation,Grant/Award Number:ZR2020ZD07Strategic Priority Research Program of Chinese Academy of Sciences,Grant/Award Number:XDA22010600。
文摘Polymer electrolytes(PEs)have been long recognized as the key materials to enable energy-dense batteries and render flexible energy devices practically viable,owing to their chemical and mechanical reliability.However,much of their promise is yet to be realized.The roomtemperature ion conductivity of existing PEs still falls short of the implementation criterion of 10^(-4) S cm^(-1) on the promise of acceptable mechanical properties,thereby precluding their practical application.The twin but inversely related duties of polymers,that is,functioning as both an ion-conducting medium and a structural backbone,underlie this issue but are less elucidated systematically.The polyacrylate(PA)family is among promising polymer matrices on account of ester polarity,electrode compatibility,chemical tunability,and mechanical durability.The extensive applicability of PA in plasticized gels,dry solids,and emerging composites makes PA-based PEs representative to illustrate the trade-off between ion conduction and mechanical strength.We herein seek to outline the stated long-standing conflict exemplified by PA-based PEs,focusing on crucial strategies toward balancing and reconciling the two mutually exclusive properties,with the intention of offering designing guidelines for next-generation PEs.
基金funded by the National Natural Science Foundation of China(No.51775185),Natural Science Foundation of Hunan Province(2022JJ90013)Scientific Research Fund of Hunan Province Education Department(18C0003)+1 种基金Research project on teaching reform in colleges and universities of Hunan Province Education Department(20190147)Hunan Normal University University-Industry Cooperation.This work is implemented at the 2011 Collaborative Innovation Center for Development and Utilization of Finance and Economics Big Data Property,Universities of Hunan Province,Open project,Grant Number 20181901CRP04.
文摘Water resources are an indispensable and valuable resource for human survival and development.Water quality predicting plays an important role in the protection and development of water resources.It is difficult to predictwater quality due to its random and trend changes.Therefore,amethod of predicting water quality which combines Auto Regressive Integrated Moving Average(ARIMA)and clusteringmodelwas proposed in this paper.By taking thewater qualitymonitoring data of a certain river basin as a sample,thewater quality Total Phosphorus(TP)index was selected as the prediction object.Firstly,the sample data was cleaned,stationary analyzed,and white noise analyzed.Secondly,the appropriate parameters were selected according to the Bayesian Information Criterion(BIC)principle,and the trend component characteristics were obtained by using ARIMA to conduct water quality predicting.Thirdly,the relationship between the precipitation and the TP index in themonitoring water field was analyzed by the K-means clusteringmethod,and the random incremental characteristics of precipitation on water quality changes were calculated.Finally,by combining with the trend component characteristics and the random incremental characteristics,the water quality prediction results were calculated.Compared with the ARIMA water quality prediction method,experiments showed that the proposed method has higher accuracy,and its Mean Absolute Error(MAE),Mean Square Error(MSE),and Mean Absolute Percentage Error(MAPE)were respectively reduced by 44.6%,56.8%,and 45.8%.
基金the National Natural Science Foundation of China(No.51775185)Natural Science Foundation of Hunan Province(No.2022JJ90013)+1 种基金Intelligent Environmental Monitoring Technology Hunan Provincial Joint Training Base for Graduate Students in the Integration of Industry and Education,and Hunan Normal University University-Industry Cooperation.the 2011 Collaborative Innovation Center for Development and Utilization of Finance and Economics Big Data Property,Universities of Hunan Province,Open Project,Grant Number 20181901CRP04.
文摘At present,water pollution has become an important factor affecting and restricting national and regional economic development.Total phosphorus is one of the main sources of water pollution and eutrophication,so the prediction of total phosphorus in water quality has good research significance.This paper selects the total phosphorus and turbidity data for analysis by crawling the data of the water quality monitoring platform.By constructing the attribute object mapping relationship,the correlation between the two indicators was analyzed and used to predict the future data.Firstly,the monthly mean and daily mean concentrations of total phosphorus and turbidity outliers were calculated after cleaning,and the correlation between them was analyzed.Secondly,the correlation coefficients of different times and frequencies were used to predict the values for the next five days,and the data trend was predicted by python visualization.Finally,the real value was compared with the predicted value data,and the results showed that the correlation between total phosphorus and turbidity was useful in predicting the water quality.
文摘Objective:To study the mechanism of lncRNA p21 inhibiting the growth and metastasis of human gastric cancer SGC7901/GES-1 cells by mediating the Wnt/β-catenin signaling pathway.Methods:Lentiviral overexpression of lncRNA-p21 in human gastric cancer SGC7901/GES-1 cell transfections was observed and analyzed for in vitro migration,invasion,cell morphology and proliferation.Besides.Wnt/β-catenin signaling pathway was tested for direct involvement in lncRNA-p21-mediated inhibition of gastric cancer cell growth and proliferation.Wnt/β-catenin signaling pathway was validated using Li-C1.Results:Gastric cancer SGC7901/GES-1 cells in the overexpression of lncRNA-p21 showed changes in stellate morphology,low invasion,or spindle-shaped morphology.LncRNA-p21 inhibited the growth and proliferation of gastric cancer SGC7901/GES-1 cells both in vivo and in vitro,and Wnt/β-catenin signaling pathway mediated the proliferation,invasion,and migration of lncRNA-p21 on gastric cancer SGC7901/GES-1 cells.Conclusion:LncRNA-p21 can inhibit the growth and metastasis of gastric cancer SGC7901/GES-1 cells in vitro and in vivo,and the inhibition of lncRNA p21 is mainly mediated by inhibiting the Wnt/β-catenin signaling pathway.
基金Baoding Science and Technology Plan Project No.2241ZF116。
文摘Objective:To elucidate the role and mechanism of cysteine protease inhibitor SN(CST1)in promoting the metastasis of gastric cancer.Methods:(1)Firstly,6 pairs of cancer and paracancer tissue samples from gastric cancer patients without distant metastasis and 5 pairs of cancer and paracancer tissue samples from gastric cancer patients with peritoneal metastasis were collected for transcriptome sequencing.Statistical analysis was performed on the sequencing results to identify significantly upregulated differential genes.(2)Another 75 pairs of cancer and paracancer tissue samples were collected from gastric cancer patients,and the protein and total RNA of gastric cancer tissue samples were extracted.Western blot and quantitative reverse transcription polymerase chain reaction(qRT-PCR)were used to detect the expression of CST1 protein and messenger RNA(mRNA)in gastric cancer and adjacent tissues.(3)The total protein and total RNA of AGS,BGC823,HGC-27,MGC803,MKN45,SGC7901,and SNU-1 gastric cancer cells and normal gastric mucosal epithelial cells GES-1 were extracted.Western blot and qRT-PCR were used to detect CST1 protein and mRNA expression.Results:(1)The significantly upregulated differential gene CST1 was screened,and the expression of CST1 in gastric cancer tissues was significantly higher than that in adjacent tissues.(2)Compared with normal gastric mucosal epithelial cells GES-1,the expression of CST1 in gastric cancer cell lines was upregulated,and the expression of CST1 in HGC-27 and SNU-1 was relatively low,while the expression of CST1 in AGS and MKN45 was relatively high.At the same time,a stable cell line of HGC-27 overexpressing CST1 and MKN45 knocking down CST1 was successfully constructed.(3)Overexpression or knockdown of CST1 did not significantly change the proliferation ability of gastric cancer cells,but can promote the migration and invasion of gastric cancer cells.(4)CST1 may promote the metastasis of gastric cancer cells by activating the epithelial-mesenchymal transition(EMT)signaling pathway.Conclusion:CST1 gene promotes the migration of gastric cancer cells,and we found through animal experiments that CST1 can affect the metastasis and invasion function of gastric cancer and is negatively correlated with the level of E-cadherin.
基金supported by a grant from the Application Basis and Front Technology Projects of Tianjin(Science and Technology Foundation of Tianjin),No.12JCYBJC18000
文摘Because the inhibition of Nogo proteins can promote neurite growth and nerve cell differentiation, a cell-scaffold complex seeded with Nogo receptor(Ng R)-silenced neural stem cells and Schwann cells may be able to improve the microenvironment for spinal cord injury repair. Previous studies have found that mild hypothermia helps to attenuate secondary damage in the spinal cord and exerts a neuroprotective effect. Here, we constructed a cell-scaffold complex consisting of a poly(D,L-lactide-co-glycolic acid)(PLGA) scaffold seeded with Ng R-silenced neural stem cells and Schwann cells, and determined the effects of mild hypothermia combined with the cell-scaffold complexes on the spinal cord hemi-transection injury in the T9 segment in rats. Compared with the PLGA group and the Ng R-silencing cells + PLGA group, hindlimb motor function and nerve electrophysiological function were clearly improved, pathological changes in the injured spinal cord were attenuated, and the number of surviving cells and nerve fibers were increased in the group treated with the Ng R-silenced cell scaffold + mild hypothermia at 34°C for 6 hours. Furthermore, fewer pathological changes to the injured spinal cord and more surviving cells and nerve fibers were found after mild hypothermia therapy than in injuries not treated with mild hypothermia. These experimental results indicate that mild hypothermia combined with Ng R gene-silenced cells in a PLGA scaffold may be an effective therapy for treating spinal cord injury.
基金the Science and Technology Foundation of Tianjin Health Bureau,No.2010KY04
文摘Inhibition of neurite growth, which is mediated by the Nogo-66 receptor (NgR), affects nerve regeneration following neural stem cell (NSC) transplantation. The present study utilized RNA interference to silence NgR gene expression in NSCs, which were subsequently transplanted into rats with traumatic brain injury. Following transplantation of NSCs transfected with small interfering RNA, typical neural cell-like morphology was detected in injured brain tissues, and was accompanied by absence of brain tissue cavity, increased growth-associated protein 43 mRNA and protein expression, and improved neurological function compared with NSC transplantation alone. Results demonstrated that NSC transplantation with silenced NgR gene promoted functional recovery following brain injury.
基金sponsored by the Science and Technology Foundation of Tianjin Health Bureau,No. 2010ky04the Application Basis and Front Technology Projects of Tianjin (Science and Technology Foundation of Tianjin),No.12JCYBJC18000
文摘Inhibition of neurite growth,which is in large part mediated by the Nogo-66 receptor,affects neural regeneration following bone marrow mesenchymal stem cell transplantation.The tissue engineering scaffold poly(D,L-lactide-co-glycolic acid) has good histocompatibility and can promote the growth of regenerating nerve fibers.The present study used small interfering RNA to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells and Schwann cells,which were subsequently transplanted with poly(D,L-lactide-co-glycolic acid) into the spinal cord lesion regions in rats.Simultaneously,rats treated with scaffold only were taken as the control group.Hematoxylin-eosin staining and immunohistochemistry revealed that at 4 weeks after transplantation,rats had good motor function of the hind limb after treatment with Nogo-66 receptor gene-silenced cells plus the poly(D,L-lactide-co-glycolic acid) scaffold compared with rats treated with scaffold only,and the number of bone marrow mesenchymal stem cells and neuron-like cells was also increased.At 8 weeks after transplantation,horseradish peroxidase tracing and transmission electron microscopy showed a large number of unmyelinated and myelinated nerve fibers,as well as intact regenerating axonal myelin sheath following spinal cord hemisection injury.These experimental findings indicate that transplantation of Nogo-66 receptor gene-silenced bone marrow mesenchymal stem cells and Schwann cells plus a poly(D,L-lactide-co-glycolic acid) scaffold can significantly enhance axonal regeneration of spinal cord neurons and improve motor function of the extremities in rats following spinal cord injury.
