Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since...Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since the Se safety range for the human body is extremely narrow,it is imperative to evaluate the genotypic responses of mungbean sprouts to Se.This study evaluated the Se enrichment capacity and interaction withflavonoids and antioxidant systems in sprouts of 20 mungbean germplasms.Selenium treatment was done by immersing mung-bean seeds in 20μM sodium selenite solution for 8 h.Afterward,the biomass,Se amounts,flavonoid(particularly vitexin and isovitexin)contents,antioxidant capacity,and key biosynthetic gene expressions were measured.Sprout Se content was 2.0-7.0μg g^(-1) DW among the 20 mungbean germplasms.Selenium treatment differentially affected the biomass,totalflavonoid,vitexin,isovitexin,antioxidant enzyme activities,and antioxidant capacities of the mungbean germplasms.Eight germplasms showed increased biomass(p<0.05),the highest increasing by 127%,but 13 did not phenotypically respond to Se treatment.Seven and six germplasms showed varied levels of vitexin and isovitexin increment after Se treatment,the highest measuring 2.67-and 2.87-folds for vitexin and isovitexin,respectively.Two mungbeanflavonoid biosynthesis genes,chalcone synthase(VrCHS)and chalcone isomerase(VrCHI)were significantly up-regulated in the germplasms with increased vitexin and isovitexin levels(p<0.05).Moreover,Se enrichment capacity was significantly correlated with the vitexin,isovitexin,and antiox-idant capacities.In conclusion,mungbean sprouts could be a useful Se-biofortified food,but the Se enrichment capacity and nutritional response must be determined for each germplasm before commercialization.展开更多
[Objective] This study aimed to investigate the variation rule of total RNA concentration during formation of microtubers of Pinellia ternata induced by sucrose. [Method] Plantlets of P. ternata were used as experimen...[Objective] This study aimed to investigate the variation rule of total RNA concentration during formation of microtubers of Pinellia ternata induced by sucrose. [Method] Plantlets of P. ternata were used as experimental materials. Total RNA concentration was determined with spectrophotometer, and the variation of total RNA was analyzed before and after formation of P. ternata microtubers treated with different concentrations of sucrose using DDRT-PCR technology. [Result] Total RNA concentration of P. ternata treated with different concentrations of sucrose varied greatly, which showed an upward trend in the first 15 d, declined from 15th d to 25th d, and slightly raised from 25th d to 30th d. Microtubers formed under various sucrose treatments showed an increase in RNA concentration after induction, and it was assumed that the treatment of high concentrations of sucrose might have activated the expression of development-related genes in P. ternata microtubers. [Conclusion] This study provides the basis for revealing the related gene expression information and molecular mechanism during formation of P. ternata microtubers induced by sucrose.展开更多
基金This study was supported by the Key Project of Natural Science Research for Colleges and Universities in Anhui Province(KJ2021A0533,2023AH050345)the Excellent Scientific Research and Innovation Team of Universities in Anhui Province(2022AH010029).
文摘Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since the Se safety range for the human body is extremely narrow,it is imperative to evaluate the genotypic responses of mungbean sprouts to Se.This study evaluated the Se enrichment capacity and interaction withflavonoids and antioxidant systems in sprouts of 20 mungbean germplasms.Selenium treatment was done by immersing mung-bean seeds in 20μM sodium selenite solution for 8 h.Afterward,the biomass,Se amounts,flavonoid(particularly vitexin and isovitexin)contents,antioxidant capacity,and key biosynthetic gene expressions were measured.Sprout Se content was 2.0-7.0μg g^(-1) DW among the 20 mungbean germplasms.Selenium treatment differentially affected the biomass,totalflavonoid,vitexin,isovitexin,antioxidant enzyme activities,and antioxidant capacities of the mungbean germplasms.Eight germplasms showed increased biomass(p<0.05),the highest increasing by 127%,but 13 did not phenotypically respond to Se treatment.Seven and six germplasms showed varied levels of vitexin and isovitexin increment after Se treatment,the highest measuring 2.67-and 2.87-folds for vitexin and isovitexin,respectively.Two mungbeanflavonoid biosynthesis genes,chalcone synthase(VrCHS)and chalcone isomerase(VrCHI)were significantly up-regulated in the germplasms with increased vitexin and isovitexin levels(p<0.05).Moreover,Se enrichment capacity was significantly correlated with the vitexin,isovitexin,and antiox-idant capacities.In conclusion,mungbean sprouts could be a useful Se-biofortified food,but the Se enrichment capacity and nutritional response must be determined for each germplasm before commercialization.
基金Supported by National Natural Science Foundation of China (30973963)Anhui Provincial Natural Science Foundation (090413252)Key Program of Natural Science Research of Anhui Provincial Universities (KJ2009A160)
文摘[Objective] This study aimed to investigate the variation rule of total RNA concentration during formation of microtubers of Pinellia ternata induced by sucrose. [Method] Plantlets of P. ternata were used as experimental materials. Total RNA concentration was determined with spectrophotometer, and the variation of total RNA was analyzed before and after formation of P. ternata microtubers treated with different concentrations of sucrose using DDRT-PCR technology. [Result] Total RNA concentration of P. ternata treated with different concentrations of sucrose varied greatly, which showed an upward trend in the first 15 d, declined from 15th d to 25th d, and slightly raised from 25th d to 30th d. Microtubers formed under various sucrose treatments showed an increase in RNA concentration after induction, and it was assumed that the treatment of high concentrations of sucrose might have activated the expression of development-related genes in P. ternata microtubers. [Conclusion] This study provides the basis for revealing the related gene expression information and molecular mechanism during formation of P. ternata microtubers induced by sucrose.
