Besides serving as therapeutic agents and building blocks for glycoprotein synthesis,homogeneous synthetic glycopeptides also benefit understanding function of specific glycoform.However,the selectivity coupling of ol...Besides serving as therapeutic agents and building blocks for glycoprotein synthesis,homogeneous synthetic glycopeptides also benefit understanding function of specific glycoform.However,the selectivity coupling of oligosaccharides to a given peptide remains challenging despite the fact that the synthesis of structure-defined complex oligosaccharides has been greatly facilitated by chemo-enzymatic based approaches.Herein,a Ca^(2+)-promoted glycosylation approach was developed to exclusively modify phenolic peptide using a panel of biologically important glycans.展开更多
A convenient and highly efficient strategy was developed for the simplification of enzymatic synthesis and purification of oligosaccharides.Glycosyl acceptors terminated with a thiol tag were extended by enzymatic mod...A convenient and highly efficient strategy was developed for the simplification of enzymatic synthesis and purification of oligosaccharides.Glycosyl acceptors terminated with a thiol tag were extended by enzymatic modular assembly(EMA)in the aqueous phase,and the desired products were captured during solid-phase workup(SPW)using commercially available thiopropyl Sepharose 6B resin through a reversible disulfide linkage.Finally,the products were released from solid-phase resin in the presence of dithio-threitol(DTT)as reducing agent and the resin could be recovered and reused.This strategy overcomes the uncertain influence of polymer-or ionic-supports commonly used in enzymatic glycosylation,and showed perfect compatibility with all 10 enzyme modules for the rapid assembly of a series of complex oligosaccharides.展开更多
基金support from the National Key R&D Program of China(2018YFE0200501)the National Natu-ral Science Foundation of China(21977055).
文摘Besides serving as therapeutic agents and building blocks for glycoprotein synthesis,homogeneous synthetic glycopeptides also benefit understanding function of specific glycoform.However,the selectivity coupling of oligosaccharides to a given peptide remains challenging despite the fact that the synthesis of structure-defined complex oligosaccharides has been greatly facilitated by chemo-enzymatic based approaches.Herein,a Ca^(2+)-promoted glycosylation approach was developed to exclusively modify phenolic peptide using a panel of biologically important glycans.
基金This project was supported by the National Key Research and Development Program of China(Nos.2021YFC2101500,2018YFA0902002)the National Natural Science Foundation of China(Nos.21877073,21807064,21778035,and 21961142016)+2 种基金Department of Science and Technology of Shandong Province(No.2020CXGC010601)the Tianjin Natural Science Foundation(No.19JCYBJC24400)the Young Scholars Program of Shandong University,Mizutani Foundation for Glycoscience,and Central Government Guide Local Science and Technology Development Funds(No.YDZX20203700002579).
文摘A convenient and highly efficient strategy was developed for the simplification of enzymatic synthesis and purification of oligosaccharides.Glycosyl acceptors terminated with a thiol tag were extended by enzymatic modular assembly(EMA)in the aqueous phase,and the desired products were captured during solid-phase workup(SPW)using commercially available thiopropyl Sepharose 6B resin through a reversible disulfide linkage.Finally,the products were released from solid-phase resin in the presence of dithio-threitol(DTT)as reducing agent and the resin could be recovered and reused.This strategy overcomes the uncertain influence of polymer-or ionic-supports commonly used in enzymatic glycosylation,and showed perfect compatibility with all 10 enzyme modules for the rapid assembly of a series of complex oligosaccharides.
