Osteoimmunomodulation is a fascinating approach for balancing osteoimmune through regulating reciprocal interactions between bone cells and immune cells[1].Implantation of the osteoimmunity-regulating biomaterials reg...Osteoimmunomodulation is a fascinating approach for balancing osteoimmune through regulating reciprocal interactions between bone cells and immune cells[1].Implantation of the osteoimmunity-regulating biomaterials regulates osteoimmune conditions in the host dynamically,thus intensifying osseointegration under physiological microenvironments[1].This perspective presents a brief overview of osteoimmunity-regulating biomaterials for augmenting bone regeneration based on a recently published study by our research team[2].展开更多
An essential concept of cancer immunotherapy is that immunogenic cell death(ICD),characterized by the release of tumor-associated antigens(TAAs)and tumor-specific antigens(TSAs)like neoantigens,danger-associated molec...An essential concept of cancer immunotherapy is that immunogenic cell death(ICD),characterized by the release of tumor-associated antigens(TAAs)and tumor-specific antigens(TSAs)like neoantigens,danger-associated molecular patterns(DAMPs),and pro-inflammatory cytokines,facilitates the presentation of TAAs and TSAs to adaptive immune cells,eliciting an emerging or reinstating a pre-existing anti-cancer immune response.展开更多
Over the past decade,nanoparticle-based therapeutic modalities have become promising strategies in cancer therapy.Selective delivery of anticancer drugs to the lesion sites is critical for elimination of the tumor and...Over the past decade,nanoparticle-based therapeutic modalities have become promising strategies in cancer therapy.Selective delivery of anticancer drugs to the lesion sites is critical for elimination of the tumor and an improved prognosis.Innovative design and advanced biointerface engineering have promoted the development of various nanocarriers for optimized drug delivery.Keeping in mind the biological framework of the tumormicroenvironment,biomembrane-camouflaged nanoplatforms have been a research focus,reflecting their superiority in cancer targeting.In this review,we summarize the development of various biomimetic cell membrane-camouflaged nanoplatforms for cancertargeted drug delivery,which are classified according to the membranes fromdifferent cells.The challenges and opportunities of the advanced biointerface engineering drug delivery nanosystems in cancer therapy are discussed.展开更多
Various calcium ion(Ca^(2+))nanomodulators are designed for multimodal cancer treatment with the mechanism of intramitochondrial Ca^(2+) overload-induced multilevel mitochondrial destruction.This perspective briefly i...Various calcium ion(Ca^(2+))nanomodulators are designed for multimodal cancer treatment with the mechanism of intramitochondrial Ca^(2+) overload-induced multilevel mitochondrial destruction.This perspective briefly introduces the development of Ca^(2+) nanomodulators in cancer therapy based on two recent studies published by our research group.展开更多
In recent years,the developed hemostatic technologies are still difficult to be applied to the hemostasis of massive arterial and visceral hemorrhage,owing to their weak hemostatic function,inferior wet tissue adhesio...In recent years,the developed hemostatic technologies are still difficult to be applied to the hemostasis of massive arterial and visceral hemorrhage,owing to their weak hemostatic function,inferior wet tissue adhesion,and low mechanical properties.Herein,a mussel-inspired supramolecular interaction-cross-linked hydrogel with robust mechanical property(308.47±29.20 kPa)and excellent hemostatic efficiency(96.5%±2.1%)was constructed as a hemostatic sealant.Typically,we combined chitosan(CS)with silk fibroin(SF)by cross-linking them through tannic acid(TA)to maintain the structural stability of the hydrogel,especially for wet tissue adhesion ability(shear adhesive strength=29.66±0.36 kPa).Compared with other materials reported previously,the obtained CS/TA/SF hydrogel yielded a lower amount of blood loss and shorter time to hemostasis in various arterial and visceral bleeding models,which could be ascribed to the synergistic effect of wound closure under wet state as well as intrinsic hemostatic activity of CS.As a superior hemostatic sealant,the unique hydrogel proposed in this work can be exploited to offer significant advantages in the acute wound and massive hemorrhage with the restrictive access of therapeutic moieties.展开更多
RNAs are involved in the crucial processes of disease progression and have emerged as powerful therapeutic targets and diagnostic biomarkers.However,efficient delivery of therapeutic RNA to the targeted location and p...RNAs are involved in the crucial processes of disease progression and have emerged as powerful therapeutic targets and diagnostic biomarkers.However,efficient delivery of therapeutic RNA to the targeted location and precise detection of RNA markers remains challenging.Recently,more and more attention has been paid to applying nucleic acid nanoassemblies in diagnosing and treating.Due to the flexibility and deformability of nucleic acids,the nanoassemblies could be fabricated with different shapes and structures.With hybridization,nucleic acid nanoassemblies,including DNA and RNA nanostructures,can be applied to enhance RNA therapeutics and diagnosis.This review briefly introduces the construction and properties of different nucleic acid nanoassemblies and their applications for RNA therapy and diagnosis and makes further prospects for their development.展开更多
Periodontitis is an inflammatory disease caused by bacterial infection directly, and the dysregulation of host immune-inflammatory response finally destroys periodontal tissues. Current treatment strategies for period...Periodontitis is an inflammatory disease caused by bacterial infection directly, and the dysregulation of host immune-inflammatory response finally destroys periodontal tissues. Current treatment strategies for periodontitis mainly involve mechanical scaling/root planing(SRP), surgical procedures,and systemic or localized delivery of antimicrobial agents. However, SRP or surgical treatment alone has unsatisfactory long-term effects and is easy to relapse. In addition, the existing drugs for local periodontal therapy do not stay in the periodontal pocket long enough and have difficulties in maintaining a steady, effective concentration to obtain a therapeutic effect, and continuous administration always causes drug resistance. Many recent studies have shown that adding bio-functional materials and drug delivery systems upregulates the therapeutic effectiveness of periodontitis. This review focuses on the role of biomaterials in periodontitis treatment and presents an overview of antibacterial therapy, host modulatory therapy, periodontal regeneration, and multifunctional regulation of periodontitis therapy. Biomaterials provide advanced approaches for periodontal therapy, and it is foreseeable that further understanding and applications of biomaterials will promote the development of periodontal therapy.展开更多
Chiral materials with the same atomic compositions exhibit different chemical,physical,and biological properties because of their distinct spatial structures.Herein,a chiral strategy was proposed to develop poly(lacti...Chiral materials with the same atomic compositions exhibit different chemical,physical,and biological properties because of their distinct spatial structures.Herein,a chiral strategy was proposed to develop poly(lactic acid)(PLA)nanoparticle as an efficient nanoadjuvant to activate adaptive anticancer immunity.Two chiral nanovaccines were prepared by directly mixing amino-terminated PLA(PLLA-NH2 or PDLA-NH2)with the model protein antigen ovalbumin(OVA).After being injected into mice subcutaneously,both nanovaccines efficiently migrated to the lymph nodes to initiate the sequential anticancer immune responses.Compared with the PLLA nanovaccine(PLLA-OVA),the PDLA one(PDLA-OVA)contributed to more robust dendritic cell(DC)maturation,antigen presentation,and T lymphocyte activation.In addition to the activation of cellular immunity,PDLA-OVA also triggered a more vigorous activation of humoral immunity,which induced the production of more anti-OVA immunoglobulin G(IgG)than PLLA-OVA.When used as prophylactic or therapeutic nanovaccine toward murine melanoma models,PDLA-OVA triggered more potent adaptive anticancer immune responses that more effectively inhibited the cancer genesis and progression,indicating the significant potential of immunologically effective PDLA nanoadjuvant in cancer immunotherapy.展开更多
The chirality of bioactive molecules is closely related to their functions.D-amino acids commonly distributed in the bacterial cell walls trigger a robust anti-infective immune response.Inspired by that,two kinds of c...The chirality of bioactive molecules is closely related to their functions.D-amino acids commonly distributed in the bacterial cell walls trigger a robust anti-infective immune response.Inspired by that,two kinds of chiral polypeptides,poly(L-phenylalanine)-block-poly(L-lysine)(PL-K)and poly(Lphenylalanine)-block-poly(D-lysine)(PD-K),were synthesized and used as nanoadjuvants of nanovaccines for cancer prevention and therapy.The amphiphilic polypeptides self-assembled into nanoparticles with a diameter of about 30 nm during ultrasonic-assisted dissolution in phosphate-buffered saline.The nanovaccines PL-K-OVA and PD-K-OVA were easily prepared by mixing solutions of PL-K or PD-K and the model antigen chicken ovalbumin(OVA),respectively,with loading efficiencies of almost 100%.Compared to PL-K-OVA,PD-K-OVA more robustly induced dendritic cell maturation,antigen cross-presentation,and adaptive immune response.More importantly,it effectively prevented and treated the OVA-expressed B16-OVA melanoma model.PD-K-OVA achieved a tumor inhibition rate of 94.9%and even 97.0%by combining with anti-PD-1 antibody.Therefore,the chiral polypeptide nanoparticles represent simple,efficient,and extensively applicable nanoadjuvants for various nanovaccines.展开更多
Dentin hypersensitivity(DH)associated with dentinal tubule exposure is one of the most common causes of toothache with a rapid onset and short duration.Medication,filling repair,laser irradiation,crown therapy,and des...Dentin hypersensitivity(DH)associated with dentinal tubule exposure is one of the most common causes of toothache with a rapid onset and short duration.Medication,filling repair,laser irradiation,crown therapy,and desensitizing toothpaste are standard clinical treatment strategies,but unsatisfactory treatment modalities are marked by long-term administration,poor dentinal tubule closure,microleakage,and the development of secondary caries.To improve the treatment efficiency of DH,numerous organic or inorganic biomaterials have been developed to relieve toothache and reverse the instability of desensitization.Biomaterials are expected to participate in dental remineralization to achieve desensitization.This review discusses various biomaterials for DH therapy based on different desensitization mechanisms,including dentinal tubule closure and dental nerve blockade,and presents a perspective on the underlying future of dentin regeneration medicine for DH therapy.展开更多
Controlled synthesis is central to obtaining polymers with accurate structures and excellent performances.Recent research in the controlled synthesis of polymers has focused on optimizing monomers,initiation systems,a...Controlled synthesis is central to obtaining polymers with accurate structures and excellent performances.Recent research in the controlled synthesis of polymers has focused on optimizing monomers,initiation systems,and reaction conditions.The satisfactory sequence,topological structure,and dispersity have been achieved to satisfy the growing demand for functional polymers.This re-view summarizes the selection of monomers of various types and structures,the innovation of initiation systems,and the optimiza-tion of reaction conditions in the controlled synthesis of polymers and discusses their challenges and opportunities.展开更多
Infectious diseases are severe public health events that threaten global health.Prophylactic vaccines have been considered as the most effective strategy to train the immune system to recognize and clear pathogenic in...Infectious diseases are severe public health events that threaten global health.Prophylactic vaccines have been considered as the most effective strategy to train the immune system to recognize and clear pathogenic infections.However,the existing vaccines against infectious diseases have several limitations,such as difficulties in mass manufacturing and storage,weak immunogenicity,and low efficiency of available adjuvants.Biomaterials,especially functional polymers,are expected to break through these bottlenecks based on the advantages of biocompatibility,degradability,controlled synthesis,easy modification,precise targeting,and immune modulation,which are excellent carriers and adjuvants of vaccines.This review mainly summarizes the application of immunologically effective polymers-enhanced vaccines against viruses-and bacteria-related infectious diseases and predicted their potential improvements.展开更多
Since 2010,there has been a much greater awareness of the problem of intoxication,which has become the most common form of accidental and intentional death.In 2020,more than 90,000 people died of intoxication in the U...Since 2010,there has been a much greater awareness of the problem of intoxication,which has become the most common form of accidental and intentional death.In 2020,more than 90,000 people died of intoxication in the United States,which surpassed deaths due to motor vehicle accidents and firearms use to become the leading external cause of mortality.展开更多
Research and development of the ideal artificial bone-substitute materials to replace autologous and allogeneic bones for repairing bone defects is still a challenge in clinical orthopedics.Recently,poly(lactic-co-gly...Research and development of the ideal artificial bone-substitute materials to replace autologous and allogeneic bones for repairing bone defects is still a challenge in clinical orthopedics.Recently,poly(lactic-co-glycolic acid)(PLGA)-based artificial bone-substitute materials are attracting increasing attention as the benefit of their suitable biocompatibility,degradability,mechanical properties,and capabilities to promote bone regeneration.In this article,we comprehensively review the artificial bone-substitute materials made from PLGA or the composites of PLGA and other organic and inorganic substances,elaborate on their applications for bone regeneration with or without bioactive factors,and prospect the challenges and opportunities in clinical bone regeneration.展开更多
Osteonecrosis,which is typically induced by trauma,glucocorticoid abuse,or alcoholism,is one of the most severe diseases in clinical orthopedics.Osteonecrosis often leads to joint destruction,and arthroplasty is event...Osteonecrosis,which is typically induced by trauma,glucocorticoid abuse,or alcoholism,is one of the most severe diseases in clinical orthopedics.Osteonecrosis often leads to joint destruction,and arthroplasty is eventually required.Enhancement of bone regeneration is a critical management strategy employed in osteonecrosis therapy.Bone tissue engineering based on engineered three-dimensional(3D)scaffolds with appropriate architecture and osteoconductive activity,alone or functionalized with bioactive factors,have been developed to enhance bone regeneration in osteonecrosis.In this review,we elaborate on the ideal properties of 3D scaffolds for enhanced bone regeneration in osteonecrosis,including biocompatibility,degradability,porosity,and mechanical performance.In addition,we summarize the development of 3D scaffolds alone or functionalized with bioactive factors for accelerating bone regeneration in osteonecrosis and discuss their prospects for translation to clinical practice.展开更多
Immunotherapy is a promising strategy to inhibit cancer progression viaactivation of the immune system. In immunotherapy, adjuvants as immunologicstimulants or delivery systems play a critical role in inducing the ant...Immunotherapy is a promising strategy to inhibit cancer progression viaactivation of the immune system. In immunotherapy, adjuvants as immunologicstimulants or delivery systems play a critical role in inducing the antitumorimmune response and decreasing the side effects of immune stimulants. Polymernanoparticles have attracted increasing attention as an indispensable componentof immunotherapy, owing to their favorable properties, such as excellentbiocompatibility and biodegradability, flexible size, high activity as immunestimulants, large surface area for binding multivalent immune ligands, and highloading capacity for immune-related components. In cancer immunotherap^polymer nanoparticles can protect cargo from the surrounding milieu, deliverthe antigens and immunostimulatory molecules to antigen-presenting cells,or stimulate robust T cell response. This review summarizes the currentadvancements in polymer nanopartide adjuvants for cancer immunotherapyand predicts their prospects in fundamental and clinical studies.展开更多
Cancer the rapy with nanoscale drug formulations has made significant progress in the past few decades.However,the selective accumulation and release of therapeutic agents in the lesion sites are still great challenge...Cancer the rapy with nanoscale drug formulations has made significant progress in the past few decades.However,the selective accumulation and release of therapeutic agents in the lesion sites are still great challenges.To this end,we developed a cRGD-decorated pH-responsive polyion complex(PIC)micelle for intracellular targeted delivery of doxorubicin(DOX)to upregulate tumor inhibition and reduce toxicity.The PIC micelle was self-assembled via the electrostatic interaction between the positively charged cRGD-modified poly(ethylene glycol)-block-poly(L-lysine)and the anionic acid-sensitive 2,3-dimethylmaleic anhydride-modified doxorubicin(DAD).The decoration of cRGD enhanced the cell internalization of PIC micelle through the specific recognition ofαvβ3 integrin on the membrane of tumor cells.The active DOX was released under intracellular acidic microenvironment after endocytosis following the decomposition of DAD.Moreover,the targeted PIC micelle exhibited enhanced inhibition efficacies toward hepatoma in vitro and in vivo compared with the insensitive controls.The smart multifunctional micelle provides a promising platform for target intracellular delivery of therapeutic agent in cancer therapy.展开更多
The osteochondral defects caused by vigorous trauma or physical disease are difficult to be managed.Tissue engineering provides a possible option to regenerate the damaged osteochondral tissues.For osteochondral recon...The osteochondral defects caused by vigorous trauma or physical disease are difficult to be managed.Tissue engineering provides a possible option to regenerate the damaged osteochondral tissues.For osteochondral reconstruction,one intact scaffold should be considered to support the regeneration of both cartilage and subchondral bone.Therefore,the biphasic scaffolds with the mimic structures of osteochondral tissues have been developed to close this chasm.A variety of biomimetic bilayer scaffolds fabricated from natural or synthetic polymers,or the ones loading with growth factors,cells,or both of them make great progresses in osteochondral defect repair.In this review,the preparation and in vitro and/or in vivo verification of bioinspired biphasic scaffolds are summarized and discussed,as well as the prospect is predicted.展开更多
基金by the National Natural Science Foundation of China(Nos.52273158,U21A2099,52022095,52073280,51973216,51873207,and 51833010)the Science and Technology Development Program of Jilin Province(Nos.20210509005RQ,20210504001GH,and 20200404182YY)+1 种基金the Special Project for City−Academy Scientific and Technological Innovation Cooperation of Changchun(No.21SH14)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.2019230).
文摘Osteoimmunomodulation is a fascinating approach for balancing osteoimmune through regulating reciprocal interactions between bone cells and immune cells[1].Implantation of the osteoimmunity-regulating biomaterials regulates osteoimmune conditions in the host dynamically,thus intensifying osseointegration under physiological microenvironments[1].This perspective presents a brief overview of osteoimmunity-regulating biomaterials for augmenting bone regeneration based on a recently published study by our research team[2].
基金The work was financially supported by the National Natural Science Foundation of China(Nos.81874233 and 51873207)the Natural Science Foundation of Hubei Province(No.2019CFB465).
文摘An essential concept of cancer immunotherapy is that immunogenic cell death(ICD),characterized by the release of tumor-associated antigens(TAAs)and tumor-specific antigens(TSAs)like neoantigens,danger-associated molecular patterns(DAMPs),and pro-inflammatory cytokines,facilitates the presentation of TAAs and TSAs to adaptive immune cells,eliciting an emerging or reinstating a pre-existing anti-cancer immune response.
基金Financially supported by the National Natural Science Foundation of China(Grant Nos.51973216,51873207,51803006,51673190,51603204,51673187,and 51520105004)the Science and Technology Development Program of Jilin Province(Grant Nos.20190201068JC,20170101102JC,and 20160414047GH)+2 种基金the Medical and Health Program of Jilin Province(Grant No.20190304047YY)the Youth Talents Promotion Project of Jilin Province(Grant No.181909)and the Youth Innovation Promotion Association of Chinese Academy of Sciences(Grant No.2019005).
文摘Over the past decade,nanoparticle-based therapeutic modalities have become promising strategies in cancer therapy.Selective delivery of anticancer drugs to the lesion sites is critical for elimination of the tumor and an improved prognosis.Innovative design and advanced biointerface engineering have promoted the development of various nanocarriers for optimized drug delivery.Keeping in mind the biological framework of the tumormicroenvironment,biomembrane-camouflaged nanoplatforms have been a research focus,reflecting their superiority in cancer targeting.In this review,we summarize the development of various biomimetic cell membrane-camouflaged nanoplatforms for cancertargeted drug delivery,which are classified according to the membranes fromdifferent cells.The challenges and opportunities of the advanced biointerface engineering drug delivery nanosystems in cancer therapy are discussed.
基金supported by the National Natural Science Foundation of China (Grant Nos. 52022095 and51873207)the Science and Technology Development Program of Jilin Province (Grant No. 20200404182YY)+1 种基金the Youth Innovation Promotion Association of Chinese Academy of Sciences (Grant No. 2019230)the China Postdoctoral Science Foundation (Grant No. 2021M691919)。
文摘Various calcium ion(Ca^(2+))nanomodulators are designed for multimodal cancer treatment with the mechanism of intramitochondrial Ca^(2+) overload-induced multilevel mitochondrial destruction.This perspective briefly introduces the development of Ca^(2+) nanomodulators in cancer therapy based on two recent studies published by our research group.
基金This work was financially supported by the National Natural Science Foundation of China(Grant No 51903050)the Natural Science Foundation of Fujian Province(Grant No.2019J01258)+2 种基金the Opening Project of State Key Laboratory of Polymer Materials Engineering(Sichuan University,Grant No.sklpme2019-4-34)the Key Program of Qingyuan Innovation Laboratory(Grant No.00221002)the Fuzhou University Testing Fund of Precious Apparatus(Grant No.2021T025).
文摘In recent years,the developed hemostatic technologies are still difficult to be applied to the hemostasis of massive arterial and visceral hemorrhage,owing to their weak hemostatic function,inferior wet tissue adhesion,and low mechanical properties.Herein,a mussel-inspired supramolecular interaction-cross-linked hydrogel with robust mechanical property(308.47±29.20 kPa)and excellent hemostatic efficiency(96.5%±2.1%)was constructed as a hemostatic sealant.Typically,we combined chitosan(CS)with silk fibroin(SF)by cross-linking them through tannic acid(TA)to maintain the structural stability of the hydrogel,especially for wet tissue adhesion ability(shear adhesive strength=29.66±0.36 kPa).Compared with other materials reported previously,the obtained CS/TA/SF hydrogel yielded a lower amount of blood loss and shorter time to hemostasis in various arterial and visceral bleeding models,which could be ascribed to the synergistic effect of wound closure under wet state as well as intrinsic hemostatic activity of CS.As a superior hemostatic sealant,the unique hydrogel proposed in this work can be exploited to offer significant advantages in the acute wound and massive hemorrhage with the restrictive access of therapeutic moieties.
基金supported by the National Science Foundation of China(No.82003689,to Mengnan Zhao,China)the Outstanding Young Scientific Talent Foundation of Sichuan Province(No.2022JDJQ0052,to Sanjun Shi,China)+3 种基金the China Postdoctoral Science Foundation(No.2021M690489,to Mengnan Zhao,China)the Project of High-Level Talents in Sichuan Province(No.003113014003,to Sanjun Shi,China)the International Postdoctoral Exchange Fellowship Program(No.YJ20200040,to Mengnan Zhao,China)the Xinglin Scholar Research Promotion Project of Chengdu University of Traditional Chinese Medicine(No.BSH2020006,to Mengnan Zhao,China).
文摘RNAs are involved in the crucial processes of disease progression and have emerged as powerful therapeutic targets and diagnostic biomarkers.However,efficient delivery of therapeutic RNA to the targeted location and precise detection of RNA markers remains challenging.Recently,more and more attention has been paid to applying nucleic acid nanoassemblies in diagnosing and treating.Due to the flexibility and deformability of nucleic acids,the nanoassemblies could be fabricated with different shapes and structures.With hybridization,nucleic acid nanoassemblies,including DNA and RNA nanostructures,can be applied to enhance RNA therapeutics and diagnosis.This review briefly introduces the construction and properties of different nucleic acid nanoassemblies and their applications for RNA therapy and diagnosis and makes further prospects for their development.
基金supported by the Hygiene and Health Appropriate Technology Promotion Project of Jilin Province (Grant No. 2020S014, China)the Science and Technology Project of Department of Finance of Jilin Province-2021 Health Talent Construction Project of Jilin Province (Grant No. jcsz2021893-1, China)。
文摘Periodontitis is an inflammatory disease caused by bacterial infection directly, and the dysregulation of host immune-inflammatory response finally destroys periodontal tissues. Current treatment strategies for periodontitis mainly involve mechanical scaling/root planing(SRP), surgical procedures,and systemic or localized delivery of antimicrobial agents. However, SRP or surgical treatment alone has unsatisfactory long-term effects and is easy to relapse. In addition, the existing drugs for local periodontal therapy do not stay in the periodontal pocket long enough and have difficulties in maintaining a steady, effective concentration to obtain a therapeutic effect, and continuous administration always causes drug resistance. Many recent studies have shown that adding bio-functional materials and drug delivery systems upregulates the therapeutic effectiveness of periodontitis. This review focuses on the role of biomaterials in periodontitis treatment and presents an overview of antibacterial therapy, host modulatory therapy, periodontal regeneration, and multifunctional regulation of periodontitis therapy. Biomaterials provide advanced approaches for periodontal therapy, and it is foreseeable that further understanding and applications of biomaterials will promote the development of periodontal therapy.
基金supported by the National Natural Science Foundation of China(52273159,52273158,U21A2099,52173149,52073280,52022095,51973216,51873207,51833010)the Science and Technology Development Program of Jilin Province(20210509005RQ,20210504001GH,20200404182YY)+1 种基金the“Special Project for City-Academy Scientific and Technological Innovation Cooperation”of Changchun(21SH14)the Youth Innovation Promotion Association of Chinese Academy of Sciences(2019230)。
文摘Chiral materials with the same atomic compositions exhibit different chemical,physical,and biological properties because of their distinct spatial structures.Herein,a chiral strategy was proposed to develop poly(lactic acid)(PLA)nanoparticle as an efficient nanoadjuvant to activate adaptive anticancer immunity.Two chiral nanovaccines were prepared by directly mixing amino-terminated PLA(PLLA-NH2 or PDLA-NH2)with the model protein antigen ovalbumin(OVA).After being injected into mice subcutaneously,both nanovaccines efficiently migrated to the lymph nodes to initiate the sequential anticancer immune responses.Compared with the PLLA nanovaccine(PLLA-OVA),the PDLA one(PDLA-OVA)contributed to more robust dendritic cell(DC)maturation,antigen presentation,and T lymphocyte activation.In addition to the activation of cellular immunity,PDLA-OVA also triggered a more vigorous activation of humoral immunity,which induced the production of more anti-OVA immunoglobulin G(IgG)than PLLA-OVA.When used as prophylactic or therapeutic nanovaccine toward murine melanoma models,PDLA-OVA triggered more potent adaptive anticancer immune responses that more effectively inhibited the cancer genesis and progression,indicating the significant potential of immunologically effective PDLA nanoadjuvant in cancer immunotherapy.
基金supported by the National Key Research and Development Program(2022YFC2603500,2022YFC2603501,2021YFC2400600,2021YFC2400603,and 2021YFC2400604)the National Natural Science Foundation of China(52273158,52273159,U21A2099,52022095,and 52073280)+2 种基金the Science and Technology Development Program of Jilin Province(20210509005RQ,20210504001GH,20200404182YY,and 20200201322JC)the Special Project for City-Academy Scientific and Technological Innovation Cooperation of Changchun(21SH14)the Youth Innovation Promotion Association of Chinese Academy of Sciences(2019230).
文摘The chirality of bioactive molecules is closely related to their functions.D-amino acids commonly distributed in the bacterial cell walls trigger a robust anti-infective immune response.Inspired by that,two kinds of chiral polypeptides,poly(L-phenylalanine)-block-poly(L-lysine)(PL-K)and poly(Lphenylalanine)-block-poly(D-lysine)(PD-K),were synthesized and used as nanoadjuvants of nanovaccines for cancer prevention and therapy.The amphiphilic polypeptides self-assembled into nanoparticles with a diameter of about 30 nm during ultrasonic-assisted dissolution in phosphate-buffered saline.The nanovaccines PL-K-OVA and PD-K-OVA were easily prepared by mixing solutions of PL-K or PD-K and the model antigen chicken ovalbumin(OVA),respectively,with loading efficiencies of almost 100%.Compared to PL-K-OVA,PD-K-OVA more robustly induced dendritic cell maturation,antigen cross-presentation,and adaptive immune response.More importantly,it effectively prevented and treated the OVA-expressed B16-OVA melanoma model.PD-K-OVA achieved a tumor inhibition rate of 94.9%and even 97.0%by combining with anti-PD-1 antibody.Therefore,the chiral polypeptide nanoparticles represent simple,efficient,and extensively applicable nanoadjuvants for various nanovaccines.
基金This work was financially supported by the Special Program for Medical and Health Professionals of Jilin Province(No.JLSWSRCZX2021-085)the Achievement Transformation Fund of the First Hospital of Jilin University(Nos.JDYYZH-2102055 and JDYYZH-2102013).
文摘Dentin hypersensitivity(DH)associated with dentinal tubule exposure is one of the most common causes of toothache with a rapid onset and short duration.Medication,filling repair,laser irradiation,crown therapy,and desensitizing toothpaste are standard clinical treatment strategies,but unsatisfactory treatment modalities are marked by long-term administration,poor dentinal tubule closure,microleakage,and the development of secondary caries.To improve the treatment efficiency of DH,numerous organic or inorganic biomaterials have been developed to relieve toothache and reverse the instability of desensitization.Biomaterials are expected to participate in dental remineralization to achieve desensitization.This review discusses various biomaterials for DH therapy based on different desensitization mechanisms,including dentinal tubule closure and dental nerve blockade,and presents a perspective on the underlying future of dentin regeneration medicine for DH therapy.
基金supported by the National Key Research and Development Program(Nos.2022YFC2603500,2021YFC2400600)the National Natural Science Foundation of China(Nos.52273158,U21A2099,52022095,52073280,51973216)+2 种基金the Science and Technology Development Program of Jjilin Province(Nos.20220204018YY,20210509005RQ,20210504001GH,20200404182YY)the Special Project for City-Academy Scientific and Technological Innovation Cooperation of Changchun(No.21SH14)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(No.2019230).
文摘Controlled synthesis is central to obtaining polymers with accurate structures and excellent performances.Recent research in the controlled synthesis of polymers has focused on optimizing monomers,initiation systems,and reaction conditions.The satisfactory sequence,topological structure,and dispersity have been achieved to satisfy the growing demand for functional polymers.This re-view summarizes the selection of monomers of various types and structures,the innovation of initiation systems,and the optimiza-tion of reaction conditions in the controlled synthesis of polymers and discusses their challenges and opportunities.
基金the National Key Research and Development Program of China(Nos.2022YFC2603500/2022YFC2603501 and 2021YFC2400600/2021YFC2400603/2021YFC2400604)the National Natural Science Foundation of China(Nos.52273158,U21A2099,52022095,51973216,and 51873207)+3 种基金the Science and Technology Development Program of Jilin Province(Nos.20220204018YY,20210509005RQ,20210504001GH,and 20200404182YY)the Youth Innovation Promotion Association of Chinese Academy of Sciences(No.2019230)the Special Project for City–Academy Scientific and Technological Innovation Cooperation of Changchun(No.21SH14)the Fundamental Research Funds for the Central Universities,JLU.
文摘Infectious diseases are severe public health events that threaten global health.Prophylactic vaccines have been considered as the most effective strategy to train the immune system to recognize and clear pathogenic infections.However,the existing vaccines against infectious diseases have several limitations,such as difficulties in mass manufacturing and storage,weak immunogenicity,and low efficiency of available adjuvants.Biomaterials,especially functional polymers,are expected to break through these bottlenecks based on the advantages of biocompatibility,degradability,controlled synthesis,easy modification,precise targeting,and immune modulation,which are excellent carriers and adjuvants of vaccines.This review mainly summarizes the application of immunologically effective polymers-enhanced vaccines against viruses-and bacteria-related infectious diseases and predicted their potential improvements.
基金This work was financially supported by the National Natural Science Foundation of China(nos.52273158 and 52022095)the Science and Technology Development Program of Jilin Province(no.20210509005RQ)the Youth Innovation Promotion Association of Chinese Academy of Sciences(no.2019230).
文摘Since 2010,there has been a much greater awareness of the problem of intoxication,which has become the most common form of accidental and intentional death.In 2020,more than 90,000 people died of intoxication in the United States,which surpassed deaths due to motor vehicle accidents and firearms use to become the leading external cause of mortality.
基金This study was financially supported by the National Natural Science Foundation of China(Grant Nos.51973216,51873207,51803006,and 51833010)the Science and Technology Development Program of Jilin Province(Grant No.20200404182YY)+1 种基金the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Grant No.2019005)the State Key Laboratory of Advanced Technology for Materials Synthesis and Processing(Wuhan University of Technology)(Grant No.2020-KF-5).
文摘Research and development of the ideal artificial bone-substitute materials to replace autologous and allogeneic bones for repairing bone defects is still a challenge in clinical orthopedics.Recently,poly(lactic-co-glycolic acid)(PLGA)-based artificial bone-substitute materials are attracting increasing attention as the benefit of their suitable biocompatibility,degradability,mechanical properties,and capabilities to promote bone regeneration.In this article,we comprehensively review the artificial bone-substitute materials made from PLGA or the composites of PLGA and other organic and inorganic substances,elaborate on their applications for bone regeneration with or without bioactive factors,and prospect the challenges and opportunities in clinical bone regeneration.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.51973216,51873207,51803006, 51833010)the Science and Technology Development Program of Jilin Province(Grant No.20190201068JC)+3 种基金the Youth Talents Promotion Project of Jilin Province(Grant No.181909)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Grant No.2019005)the Special Foundation for Provincial Authorities from Finance Department of Jilin Province(Grant No.3D518V313429)the State Key Laboratory of Advanced Technology for Materials Synthesis and Processing(Wuhan University of Technology)(Grant No.2020-KF-5).
文摘Osteonecrosis,which is typically induced by trauma,glucocorticoid abuse,or alcoholism,is one of the most severe diseases in clinical orthopedics.Osteonecrosis often leads to joint destruction,and arthroplasty is eventually required.Enhancement of bone regeneration is a critical management strategy employed in osteonecrosis therapy.Bone tissue engineering based on engineered three-dimensional(3D)scaffolds with appropriate architecture and osteoconductive activity,alone or functionalized with bioactive factors,have been developed to enhance bone regeneration in osteonecrosis.In this review,we elaborate on the ideal properties of 3D scaffolds for enhanced bone regeneration in osteonecrosis,including biocompatibility,degradability,porosity,and mechanical performance.In addition,we summarize the development of 3D scaffolds alone or functionalized with bioactive factors for accelerating bone regeneration in osteonecrosis and discuss their prospects for translation to clinical practice.
基金This research was financially supported by the National Natural Science Foundation of China （Nos. 51673190, 51603204, 51673187, 51773083, 51503076, and 51520105004） and the Science and Technology Development Program of Jilin Province （Nos. 20160204015SF and 20160204018SF）.
文摘Immunotherapy is a promising strategy to inhibit cancer progression viaactivation of the immune system. In immunotherapy, adjuvants as immunologicstimulants or delivery systems play a critical role in inducing the antitumorimmune response and decreasing the side effects of immune stimulants. Polymernanoparticles have attracted increasing attention as an indispensable componentof immunotherapy, owing to their favorable properties, such as excellentbiocompatibility and biodegradability, flexible size, high activity as immunestimulants, large surface area for binding multivalent immune ligands, and highloading capacity for immune-related components. In cancer immunotherap^polymer nanoparticles can protect cargo from the surrounding milieu, deliverthe antigens and immunostimulatory molecules to antigen-presenting cells,or stimulate robust T cell response. This review summarizes the currentadvancements in polymer nanopartide adjuvants for cancer immunotherapyand predicts their prospects in fundamental and clinical studies.
基金financially supported by the National Natural Science Foundation of China(Nos.51973216,51873207,51833010,51703225,51673190,51673187,51603204 and 51520105004)the Science and Technology Development Program of Jilin Province(No.20190201068JC)+2 种基金the National Key Research and Development Program of China(No.2016YFC1100701)the Youth Talents Promotion Project of Jilin Province(No.181909)the Youth Innovation Promotion Association of Chinese Academy of Sciences(No.2019005)。
文摘Cancer the rapy with nanoscale drug formulations has made significant progress in the past few decades.However,the selective accumulation and release of therapeutic agents in the lesion sites are still great challenges.To this end,we developed a cRGD-decorated pH-responsive polyion complex(PIC)micelle for intracellular targeted delivery of doxorubicin(DOX)to upregulate tumor inhibition and reduce toxicity.The PIC micelle was self-assembled via the electrostatic interaction between the positively charged cRGD-modified poly(ethylene glycol)-block-poly(L-lysine)and the anionic acid-sensitive 2,3-dimethylmaleic anhydride-modified doxorubicin(DAD).The decoration of cRGD enhanced the cell internalization of PIC micelle through the specific recognition ofαvβ3 integrin on the membrane of tumor cells.The active DOX was released under intracellular acidic microenvironment after endocytosis following the decomposition of DAD.Moreover,the targeted PIC micelle exhibited enhanced inhibition efficacies toward hepatoma in vitro and in vivo compared with the insensitive controls.The smart multifunctional micelle provides a promising platform for target intracellular delivery of therapeutic agent in cancer therapy.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.51303174,51273196,51203153,51233004,51390484,and 51321062)the Scientific Development Program of Jilin Province(Nos.20140520050JH and 20140309005GX)the Science and Technology Planning Project of Changchun City(No.14KG045).
文摘The osteochondral defects caused by vigorous trauma or physical disease are difficult to be managed.Tissue engineering provides a possible option to regenerate the damaged osteochondral tissues.For osteochondral reconstruction,one intact scaffold should be considered to support the regeneration of both cartilage and subchondral bone.Therefore,the biphasic scaffolds with the mimic structures of osteochondral tissues have been developed to close this chasm.A variety of biomimetic bilayer scaffolds fabricated from natural or synthetic polymers,or the ones loading with growth factors,cells,or both of them make great progresses in osteochondral defect repair.In this review,the preparation and in vitro and/or in vivo verification of bioinspired biphasic scaffolds are summarized and discussed,as well as the prospect is predicted.
