Objective: To understand the effect of humanistic nursing care model wards in Children Caring Ward School(CCWS) on the nurses' caring ability.Methods: Questionnaire 25 nurses of humanistic nursing care model wards...Objective: To understand the effect of humanistic nursing care model wards in Children Caring Ward School(CCWS) on the nurses' caring ability.Methods: Questionnaire 25 nurses of humanistic nursing care model wards in CCWS using the Nkongho Caring Ability Inventory(CAI) before and after implement the humanistic nursing care model, including reform the systems of nursing care, introduce humanistic care model, implement the humanistic care, to measure the nurses' caring ability.Results: The nurses' caring ability had significantly developed on total, cognition dimension, courage dimension and patience dimension after all measures considered(p < 0.05).Conclusions: The humanistic nursing care model wards in CCWS has a positive effect on the nurses' caring ability, not only to help build great relationships between nurses and patients but also to enhance the patients' satisfaction.展开更多
Chrysanthemum indicum Flower is usually consumed as functional food. This paper described an improved total quality assessment method for Chrysanthemum indicum Flower by simultaneous quantitation using a single standa...Chrysanthemum indicum Flower is usually consumed as functional food. This paper described an improved total quality assessment method for Chrysanthemum indicum Flower by simultaneous quantitation using a single standard to determine multi-components method combined with high performance liquid chromatography fingerprint analysis. Six main components of Chrysanthemum indicum Flower including two flavonoids and four phenolic acids were simultaneously quantified using linarin as the internal reference standard. The method was fully validated with respect to linearity, precision, accuracy, robustness and stability.The validated method was successfully applied to the analysis of thirty three batches of Chrysanthemum indicum Flower samples. Under the same chromatographic conditions, fingerprint analysis in combination with Similarity analysis and principal component analysis was performed to identify the samples from different regions. In general, an effective assessment using a single standard to determinate multi-components method combined with fingerprint analysis make the reliable qualitation and quantitation analysis of Chrysanthemum indicum Flower available.展开更多
[Objectives]Proteomics methods were used to analyze the total proteome of Holothuria leucospilota in the South China Sea,so as to provide a theoretical basis for elucidating the medicinal material basis of sea cucumbe...[Objectives]Proteomics methods were used to analyze the total proteome of Holothuria leucospilota in the South China Sea,so as to provide a theoretical basis for elucidating the medicinal material basis of sea cucumber proteins and peptides.[Methods]H.leucospilota was collected from the South China Sea.Label-free proteomics methods were used to detect the protein profile of enzymatically hydrolyzed H.leucospilota total protein,and identify sea cucumber protein and peptide sequences,and gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)bioinformatics analysis software were used to analyze significantly enriched proteins.The String database was used to construct a protein-protein interaction network of significantly enriched H.leucospilota proteins(PPI network).[Results]Proteomics identified 1200 sea cucumber proteins and 2967 peptides.GO analysis and KEGG analysis showed that the four significantly-enriched sea cucumber proteins hsc70,COX,CYTB and rbbp4 participated in 8 metabolic pathways,and COX1 and CYTB among them were significantly related to cardiac muscle contraction,oxidative phosphorylation and metabolic pathways.The PPI network analysis showed that the 13 significantly-enriched sea cucumber proteins had close protein-protein interaction relationships.[Conclusions]This study used proteomics to conduct preliminary identification and research on the proteins and peptides of H.leucospilota,providing a scientific basis for the application of H.leucospilota proteins and peptides in the fields of functional health food and drug research and development.展开更多
目的探讨双平板数字减影血管造影(DSA)机在全脑血管造影中的应用价值。方法将拟行脑血管造影的患者,随机分为实验组(n=50,采用双平板DSA)和对照组(n=59,采用单平板DSA),对比两组患者的手术时间、对比剂用量、电影序列数、摄影帧数、剂...目的探讨双平板数字减影血管造影(DSA)机在全脑血管造影中的应用价值。方法将拟行脑血管造影的患者,随机分为实验组(n=50,采用双平板DSA)和对照组(n=59,采用单平板DSA),对比两组患者的手术时间、对比剂用量、电影序列数、摄影帧数、剂量面积乘积以及空气比释动能。结果实验组手术时间[33.50(25.00,40.50)min vs 45.00(40.00,50.00)min,P<0.001]、对比剂用量(62.68±22.40 mL vs 100.46±20.91 mL,P<0.001)、以及电影序列数[10.00(8.00,13.25)vs 14.00(12.00,16.00),P<0.001]均低于对照组,差异有统计学意义。两组电影序列数均与手术时间(r=0.586,P<0.001)、术中对比剂用量(r=0.637,P<0.001)成正相关。两组患者在剂量面积乘积、空气比释动能、摄影帧数的差异无统计学意义(P>0.05)。结论采用双平板DSA机造影的辐射剂量与常规采用单平板DSA无明显差别,但使用双平板DSA造影,手术时间以及术中对比剂用量明显减少,有益于患者。展开更多
Laser-induced breakdown spectroscopy has become a general-purpose technique, and internal standard calibration is a common method for quantitative analysis. Calibration models should be reconstructed for different sys...Laser-induced breakdown spectroscopy has become a general-purpose technique, and internal standard calibration is a common method for quantitative analysis. Calibration models should be reconstructed for different systems and application environments. This study presents an efficient procedure in the construction and selection of calibration models for LIBS analysis. The procedure concludes data preprocess, calibration model construction, and concentration calculation. These steps can be programmed without manual intervention. Results of the quantitative analysis of Ni-based alloys using the proposed procedure are presented in this study.Ten elements are calibrated, and most have an average relative standard error of less than 10%.The proposed procedure is an effective process for constructing and selecting calibration models.展开更多
The effective removal and selective detection of explosive and toxic pollutant trinitrophenol (TNP) is an attractive but challenging field.Herein,a double-cavity nor-seco-cucurbit[10]uril (ns-Q[10])-based supramolecul...The effective removal and selective detection of explosive and toxic pollutant trinitrophenol (TNP) is an attractive but challenging field.Herein,a double-cavity nor-seco-cucurbit[10]uril (ns-Q[10])-based supramolecular assembly 8-HQ@ns-Q[10]was fabricated and its structure was characterized by X-ray single crystal diffraction.In this assembly,the stoichiometric ratio of ns-Q[10]and 8-hydroxyquinoline (8-HQ) is 1:2,which is also attributed to the special double-cavity structure of ns-Q[10].The luminescence sensing experiments showed that 8-HQ@ns-Q[10]can be used as a good fluorescence-enhanced sensing material (enhanced 27-fold) for the rapid detection of explosives and the aqueous contaminant TNP,with a limit of detection (LOD) of 2.07×10^(-5)mol/L,without interference from other phenolic compounds.Furthermore,TNP can be efficiently removed in the presence of assembly 8-HQ@ns-Q[10],and the removal efficiency is more than 89%.Therefore,the supramolecular assembly 8-HQ@ns-Q[10],as a fluorescenceenhanced luminescence sensor and adsorption material,has rich research value and potential application prospect when applied to the detection and removal of TNP in aqueous environment.展开更多
Graphene as a two-dimensional material is prone to hydrocarbon contaminations,which can significantly alter its intrinsic electrical properties.Herein,we implement a facile hydrogenation-dehydrogenation strategy to re...Graphene as a two-dimensional material is prone to hydrocarbon contaminations,which can significantly alter its intrinsic electrical properties.Herein,we implement a facile hydrogenation-dehydrogenation strategy to remove hydrocarbon contaminations and preserve the excellent transport properties of monolayer graphene.Using electron microscopy we quantitatively characterized the improved cleanness of hydrogenated graphene compared to untreated samples.In situ spectroscopic investigations revealed that the hydrogenation treatment promoted the adsorption of water at the graphene surface,resulting in a protective layer against the re-deposition of hydrocarbon molecules.Additionally,the further dehydrogenation of hydrogenated graphene rendered a more pristine-like basal plane with improved carrier mobility compared to untreated pristine graphene.Our findings provide a practical post-growth cleaning protocol for graphene with maintained surface cleanness and lattice integrity to systematically carry a range of surface chemistry in the form of a well-performing and reproducible transistor.展开更多
Cyanobacteria, which occurred in eutrophic water harvest solar light to carry out photosynthesis with high efficiency. In this work, cyanobacteria(Microcystis sp.) were used as biotemplate to synthesize titania struct...Cyanobacteria, which occurred in eutrophic water harvest solar light to carry out photosynthesis with high efficiency. In this work, cyanobacteria(Microcystis sp.) were used as biotemplate to synthesize titania structure. The synthesized titania sample had similar morphology to that of the original template in spite of the fragile unicellular structures and extremely high water content of cyanobacterial cells. Incorporation of biogenic C, as well as the morphology inherited from biotemplate improved visiblelight absorbance of the titania structure. The sample exhibited higher visible-light photocatalytic activity than commercial titania photocatalyst Degussa P25 for Rhodamine B(RhB) degradation. Compared with those C-doped titania photocatalysts prepared by other methods, cyanobacteria templated titania photocatalyst offer some potential for competitive advantages. The reported strategy opened up a new use for the cyanobacteria. It could also be used for titania in applications such as treatment of polluted water, dye-sensitized solar cells, or other regions.展开更多
基金supported by the Fundamental Research Funds for the Central Universities,Huazhong University of Science and Technology(No.2014LC028)
文摘Objective: To understand the effect of humanistic nursing care model wards in Children Caring Ward School(CCWS) on the nurses' caring ability.Methods: Questionnaire 25 nurses of humanistic nursing care model wards in CCWS using the Nkongho Caring Ability Inventory(CAI) before and after implement the humanistic nursing care model, including reform the systems of nursing care, introduce humanistic care model, implement the humanistic care, to measure the nurses' caring ability.Results: The nurses' caring ability had significantly developed on total, cognition dimension, courage dimension and patience dimension after all measures considered(p < 0.05).Conclusions: The humanistic nursing care model wards in CCWS has a positive effect on the nurses' caring ability, not only to help build great relationships between nurses and patients but also to enhance the patients' satisfaction.
文摘Chrysanthemum indicum Flower is usually consumed as functional food. This paper described an improved total quality assessment method for Chrysanthemum indicum Flower by simultaneous quantitation using a single standard to determine multi-components method combined with high performance liquid chromatography fingerprint analysis. Six main components of Chrysanthemum indicum Flower including two flavonoids and four phenolic acids were simultaneously quantified using linarin as the internal reference standard. The method was fully validated with respect to linearity, precision, accuracy, robustness and stability.The validated method was successfully applied to the analysis of thirty three batches of Chrysanthemum indicum Flower samples. Under the same chromatographic conditions, fingerprint analysis in combination with Similarity analysis and principal component analysis was performed to identify the samples from different regions. In general, an effective assessment using a single standard to determinate multi-components method combined with fingerprint analysis make the reliable qualitation and quantitation analysis of Chrysanthemum indicum Flower available.
基金Supported by Scientific Research Program of Higher Education Institutions in Hainan Province(Hnky2019-110).
文摘[Objectives]Proteomics methods were used to analyze the total proteome of Holothuria leucospilota in the South China Sea,so as to provide a theoretical basis for elucidating the medicinal material basis of sea cucumber proteins and peptides.[Methods]H.leucospilota was collected from the South China Sea.Label-free proteomics methods were used to detect the protein profile of enzymatically hydrolyzed H.leucospilota total protein,and identify sea cucumber protein and peptide sequences,and gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)bioinformatics analysis software were used to analyze significantly enriched proteins.The String database was used to construct a protein-protein interaction network of significantly enriched H.leucospilota proteins(PPI network).[Results]Proteomics identified 1200 sea cucumber proteins and 2967 peptides.GO analysis and KEGG analysis showed that the four significantly-enriched sea cucumber proteins hsc70,COX,CYTB and rbbp4 participated in 8 metabolic pathways,and COX1 and CYTB among them were significantly related to cardiac muscle contraction,oxidative phosphorylation and metabolic pathways.The PPI network analysis showed that the 13 significantly-enriched sea cucumber proteins had close protein-protein interaction relationships.[Conclusions]This study used proteomics to conduct preliminary identification and research on the proteins and peptides of H.leucospilota,providing a scientific basis for the application of H.leucospilota proteins and peptides in the fields of functional health food and drug research and development.
文摘目的探讨双平板数字减影血管造影(DSA)机在全脑血管造影中的应用价值。方法将拟行脑血管造影的患者,随机分为实验组(n=50,采用双平板DSA)和对照组(n=59,采用单平板DSA),对比两组患者的手术时间、对比剂用量、电影序列数、摄影帧数、剂量面积乘积以及空气比释动能。结果实验组手术时间[33.50(25.00,40.50)min vs 45.00(40.00,50.00)min,P<0.001]、对比剂用量(62.68±22.40 mL vs 100.46±20.91 mL,P<0.001)、以及电影序列数[10.00(8.00,13.25)vs 14.00(12.00,16.00),P<0.001]均低于对照组,差异有统计学意义。两组电影序列数均与手术时间(r=0.586,P<0.001)、术中对比剂用量(r=0.637,P<0.001)成正相关。两组患者在剂量面积乘积、空气比释动能、摄影帧数的差异无统计学意义(P>0.05)。结论采用双平板DSA机造影的辐射剂量与常规采用单平板DSA无明显差别,但使用双平板DSA造影,手术时间以及术中对比剂用量明显减少,有益于患者。
基金financial support provided by National Natural Science Foundation of China (11704372)Anhui Provincial Natural Science Foundation (1708085QF130)
文摘Laser-induced breakdown spectroscopy has become a general-purpose technique, and internal standard calibration is a common method for quantitative analysis. Calibration models should be reconstructed for different systems and application environments. This study presents an efficient procedure in the construction and selection of calibration models for LIBS analysis. The procedure concludes data preprocess, calibration model construction, and concentration calculation. These steps can be programmed without manual intervention. Results of the quantitative analysis of Ni-based alloys using the proposed procedure are presented in this study.Ten elements are calibrated, and most have an average relative standard error of less than 10%.The proposed procedure is an effective process for constructing and selecting calibration models.
基金the support of the Natural Science Foundation of the Science and Technology Department of Guizhou Province(No.ZK-2021-024)the National Natural Science Foundation of China(No.22061009)the Innovation Program for High-level Talents of Guizhou Province(No.2016-5657)。
文摘The effective removal and selective detection of explosive and toxic pollutant trinitrophenol (TNP) is an attractive but challenging field.Herein,a double-cavity nor-seco-cucurbit[10]uril (ns-Q[10])-based supramolecular assembly 8-HQ@ns-Q[10]was fabricated and its structure was characterized by X-ray single crystal diffraction.In this assembly,the stoichiometric ratio of ns-Q[10]and 8-hydroxyquinoline (8-HQ) is 1:2,which is also attributed to the special double-cavity structure of ns-Q[10].The luminescence sensing experiments showed that 8-HQ@ns-Q[10]can be used as a good fluorescence-enhanced sensing material (enhanced 27-fold) for the rapid detection of explosives and the aqueous contaminant TNP,with a limit of detection (LOD) of 2.07×10^(-5)mol/L,without interference from other phenolic compounds.Furthermore,TNP can be efficiently removed in the presence of assembly 8-HQ@ns-Q[10],and the removal efficiency is more than 89%.Therefore,the supramolecular assembly 8-HQ@ns-Q[10],as a fluorescenceenhanced luminescence sensor and adsorption material,has rich research value and potential application prospect when applied to the detection and removal of TNP in aqueous environment.
基金This work was supported by the Chinese Scholarship Council(201406890016)NWA route‘meten&detecteren’+6 种基金the European Research Council under the European Union’s Seventh Framework Program(FP/2007-2013)/ERC Grant Agreement No.335879 project acronym‘Biographene’the Netherlands Organization for Scientific Research(Vidi 723.013.007)L.Wu and J.P.Hofmann acknowledge funding from The Netherlands Organization for Scientific Research(NWO)cofinancing by Shell Global Solutions International B.V.for the project 13CO2-6E.J.M.H.and V.M.acknowledge support by the Netherlands Center for Multiscale Catalytic Energy Conversion(MCEC)an NWO Gravitation program funded by the Ministry of Education,Culture and Science of the government of the Netherlandsa Vici grant of the NWO.
文摘Graphene as a two-dimensional material is prone to hydrocarbon contaminations,which can significantly alter its intrinsic electrical properties.Herein,we implement a facile hydrogenation-dehydrogenation strategy to remove hydrocarbon contaminations and preserve the excellent transport properties of monolayer graphene.Using electron microscopy we quantitatively characterized the improved cleanness of hydrogenated graphene compared to untreated samples.In situ spectroscopic investigations revealed that the hydrogenation treatment promoted the adsorption of water at the graphene surface,resulting in a protective layer against the re-deposition of hydrocarbon molecules.Additionally,the further dehydrogenation of hydrogenated graphene rendered a more pristine-like basal plane with improved carrier mobility compared to untreated pristine graphene.Our findings provide a practical post-growth cleaning protocol for graphene with maintained surface cleanness and lattice integrity to systematically carry a range of surface chemistry in the form of a well-performing and reproducible transistor.
基金supported by the National Natural Science Foundation of China(Project NSFC-YN U1033603,21063016)the Program for Innovative Research Teams (in Science and Technology) in the University of Yunnan Province(IRTSTYN)
文摘Cyanobacteria, which occurred in eutrophic water harvest solar light to carry out photosynthesis with high efficiency. In this work, cyanobacteria(Microcystis sp.) were used as biotemplate to synthesize titania structure. The synthesized titania sample had similar morphology to that of the original template in spite of the fragile unicellular structures and extremely high water content of cyanobacterial cells. Incorporation of biogenic C, as well as the morphology inherited from biotemplate improved visiblelight absorbance of the titania structure. The sample exhibited higher visible-light photocatalytic activity than commercial titania photocatalyst Degussa P25 for Rhodamine B(RhB) degradation. Compared with those C-doped titania photocatalysts prepared by other methods, cyanobacteria templated titania photocatalyst offer some potential for competitive advantages. The reported strategy opened up a new use for the cyanobacteria. It could also be used for titania in applications such as treatment of polluted water, dye-sensitized solar cells, or other regions.
