The characteristics of zinc finger of Kruppel homolog-1 (N/kr-hl) gene from brown planthopper and its effect on expression of FAMET and JHE were studied in the paper, in order to understand the regulation role of Nl...The characteristics of zinc finger of Kruppel homolog-1 (N/kr-hl) gene from brown planthopper and its effect on expression of FAMET and JHE were studied in the paper, in order to understand the regulation role of Nlkr-hl. The evolutionary tree of each zinc finger in functional area of Nlkr-hl was analyzed via MEGAS. 0 software, and expression status of FAMeT and JHE after interference of N/kr-hl was determined using qPCR technology. The results showed that the functional area of N/kr-hl mainly included eight zinc fingers, and the conservativeness of Znl and Zn8 was relatively lower. In the brown planthopper with N/b'-hl gene knockout, the expression levels of FAMeT and JHE increased. The paper provided the theoretical basis for understanding molecular mechanism of juvenile hormone signaling pathway and biological control of brown planthopper.展开更多
文摘The characteristics of zinc finger of Kruppel homolog-1 (N/kr-hl) gene from brown planthopper and its effect on expression of FAMET and JHE were studied in the paper, in order to understand the regulation role of Nlkr-hl. The evolutionary tree of each zinc finger in functional area of Nlkr-hl was analyzed via MEGAS. 0 software, and expression status of FAMeT and JHE after interference of N/kr-hl was determined using qPCR technology. The results showed that the functional area of N/kr-hl mainly included eight zinc fingers, and the conservativeness of Znl and Zn8 was relatively lower. In the brown planthopper with N/b'-hl gene knockout, the expression levels of FAMeT and JHE increased. The paper provided the theoretical basis for understanding molecular mechanism of juvenile hormone signaling pathway and biological control of brown planthopper.