Objective:To study the effect of aspirin on healing process of osteoporotic fracture(OPF)in rats.Methods:A total of 50 female Wistar rata aged 3 months were randomly divided into observation group and control group,ca...Objective:To study the effect of aspirin on healing process of osteoporotic fracture(OPF)in rats.Methods:A total of 50 female Wistar rata aged 3 months were randomly divided into observation group and control group,castration method was adopted to establish the osteoporosis(OP)model.After artificial preparing fractures on the midpoint of left femur,fixing gram needle intramedullary.OPF modeling was complete.Aspirin lavage of 33 mg once a day was adoptde in observation group after modeling,same amount of normal saline was used in the control as placebo.From eash group,selected 5 rats at the 2nd.4th,8th and 12th week after modeling to measure the bone mineral density(BMD)and histogical examination of the fracture callus,radiology observation was conducted at the 8th and 12th week.Left femur biomechanical measurement was taken at the 12th week.Results:BMD values of observation group at each time point were significantly higher than that of the control group after modeling(P<0.05);Histological observation showed that at the 8th week,the endochondral ossification process of observation group was faster than that of observation group,with fuzzy fracture line in observation group and clear fracture line in observtion group;at the 12th week,fracture line disappeared in observation group,fracture line of the control group was fuzzy at the same time;three-point bending load of the left femur in observation group rats was significantly higher than that of control group after12 weeka(P<0.05).Conclusions:Asporin can accelerate the healing of new callus in OPF rats,increase bone density and biomechanics strength,and promote fracture of osteporotic rats.展开更多
As an innovative in vitro culture model,organoids have been established by cell sorting and subsequent culture in three-dimensional culture systems.Organoids can be derived from induced pluripotent stem cells or organ...As an innovative in vitro culture model,organoids have been established by cell sorting and subsequent culture in three-dimensional culture systems.Organoids can be derived from induced pluripotent stem cells or organ-restricted adult stem cells.Compared with traditional two-dimensional cell culture models and patient-derived xenograft models,organoids possess long-term genetic stability and can better retain the characteristics of source tissues or organs.These advantages have led to the increased use of ovarian and fallopian tube organoids in various fields of research,including cell differentiation and development,establishment of disease occurrence and progression models,tissue regeneration and reconstruction,individual drug screening,immune cell co-culture,and maternal-fetal medicine.This review briefly summarizes the recent progress in the application of ovarian and fallopian tube organoids in the field of obstetrics and gynecology.展开更多
基金supported by Guangdong Science and Technology Projects,grant No.2010b031600288
文摘Objective:To study the effect of aspirin on healing process of osteoporotic fracture(OPF)in rats.Methods:A total of 50 female Wistar rata aged 3 months were randomly divided into observation group and control group,castration method was adopted to establish the osteoporosis(OP)model.After artificial preparing fractures on the midpoint of left femur,fixing gram needle intramedullary.OPF modeling was complete.Aspirin lavage of 33 mg once a day was adoptde in observation group after modeling,same amount of normal saline was used in the control as placebo.From eash group,selected 5 rats at the 2nd.4th,8th and 12th week after modeling to measure the bone mineral density(BMD)and histogical examination of the fracture callus,radiology observation was conducted at the 8th and 12th week.Left femur biomechanical measurement was taken at the 12th week.Results:BMD values of observation group at each time point were significantly higher than that of the control group after modeling(P<0.05);Histological observation showed that at the 8th week,the endochondral ossification process of observation group was faster than that of observation group,with fuzzy fracture line in observation group and clear fracture line in observtion group;at the 12th week,fracture line disappeared in observation group,fracture line of the control group was fuzzy at the same time;three-point bending load of the left femur in observation group rats was significantly higher than that of control group after12 weeka(P<0.05).Conclusions:Asporin can accelerate the healing of new callus in OPF rats,increase bone density and biomechanics strength,and promote fracture of osteporotic rats.
文摘As an innovative in vitro culture model,organoids have been established by cell sorting and subsequent culture in three-dimensional culture systems.Organoids can be derived from induced pluripotent stem cells or organ-restricted adult stem cells.Compared with traditional two-dimensional cell culture models and patient-derived xenograft models,organoids possess long-term genetic stability and can better retain the characteristics of source tissues or organs.These advantages have led to the increased use of ovarian and fallopian tube organoids in various fields of research,including cell differentiation and development,establishment of disease occurrence and progression models,tissue regeneration and reconstruction,individual drug screening,immune cell co-culture,and maternal-fetal medicine.This review briefly summarizes the recent progress in the application of ovarian and fallopian tube organoids in the field of obstetrics and gynecology.
