乙型肝炎病毒(hepatitis B virus,HBV)合成的蛋白调节细胞脂质代谢的研究不断被报道,但乙型肝炎病毒表面抗原(hepatitis B virus surface antigen,HBsAg)与脂质代谢的相互调控研究较少,且机制尚不明确。本研究通过对细胞转录组学的分析...乙型肝炎病毒(hepatitis B virus,HBV)合成的蛋白调节细胞脂质代谢的研究不断被报道,但乙型肝炎病毒表面抗原(hepatitis B virus surface antigen,HBsAg)与脂质代谢的相互调控研究较少,且机制尚不明确。本研究通过对细胞转录组学的分析,揭示HBsAg对脂质代谢的调控机制。选用稳定表达HBsAg的细胞系HepG2-S-G2与其对照细胞系HepG2-neo-F4进行转录组学分析。利用定量聚合酶链反应(polymerase chain reaction,PCR)、蛋白质印迹法(Western blot,WB)分别检测重要差异基因OXCT1和CYP4F3在mRNA水平和蛋白水平的表达差异。为验证HBsAg促进脂质合成上调的表型,对两种细胞系进行油红O染色并检测细胞脂肪酸、总胆固醇水平。进一步对稳定转染HBV的细胞系HepG2.2.15进行降脂处理,以观察细胞上清液中HBsAg与脂质合成之间是否存在相互调控。结果显示,参与脂质代谢的差异基因发生显著变化,提示HBsAg引起了宿主细胞脂质合成途径的上调和消耗途径下调。定量PCR结果显示,相对于HepG2-neo-F4细胞,HepG2-S-G2细胞的3-酮酸辅酶A转移酶1(3-oxoacid CoA-transferase 1,OXCT1)mRNA水平升高约9倍,与转录组测序结果基本一致;CYP4F3基因在HepG2-S-G2细胞中转录相对下调。WB结果显示,OXCT1和CYP4F3蛋白表达均出现相应的显著上调或下调,并且趋势与转录组分析一致。油红O染色以及细胞脂肪酸、总胆固醇水平检测结果证实HepG2-S-G2细胞中脂滴更明显,且游离脂肪酸和总胆固醇均显著升高。降脂处理结果显示细胞上清液中HBsAg显著降低。上述结果表明,HBsAg可上调脂质代谢、促进脂质合成,提示降脂可能成为抑制HBsAg的潜在有效途径。展开更多
Genistein is effective against amyloid-β toxicity, but the underlying mechanisms are unclear. We hypothesized that genistein may protect neurons by inhibiting the mitochondrial apoptotic pathway, and thereby play a r...Genistein is effective against amyloid-β toxicity, but the underlying mechanisms are unclear. We hypothesized that genistein may protect neurons by inhibiting the mitochondrial apoptotic pathway, and thereby play a role in the prevention of Alzheimer＇s disease. A rat model of Alzheimer＇s disease was established by intraperitoneal injection of D-galactose and intracerebral injection of amyloid-β peptide （25-35）. In the genistein treatment groups, a 7-day pretreatment with genistein （10, 30, 90 mg/kg） was given prior to establishing Alzheimer＇s disease model, for 49 consecutive days. Terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling assay demonstrated a reduction in apoptosis in the hippocampus of rats treated with genistein. Western blot analysis showed that expression levels of capase-3, Bax and cytochrome c were decreased compared with the model group. Furthermore, immunohistochemical staining revealed reductions in cytochrome c and Bax immunoreactivity in these rats. Morris water maze revealed a substantial shortening of escape latency by genistein in Alzheimer＇s disease rats. These findings suggest that genistein decreases neuronal loss in the hippocampus, and improves learning and memory ability. The neuroprotective effects of genistein are associated with the inhibition of the mitochondrial apoptotic pathway, as shown by its ability to reduce levels of caspase-3, Bax and cytochrome c.展开更多
In recent years, the number of Web services has increased significantly. Web service discovery has drawn much attention with the development of Web service applications and big data analysis. Under this circumstance, ...In recent years, the number of Web services has increased significantly. Web service discovery has drawn much attention with the development of Web service applications and big data analysis. Under this circumstance, traditional Web service discovery strategies cannot adequately meet high user requirements due to the efficiency and precision of service discovery is low. In order to improve the accuracy and efficiency of service discovery, a user requirement oriented Web service discovery approach based on Petri nets is proposed in this study. A data preprocessing strategy of Web service is first designed. Then, a service clustering method is proposed based on Petri nets, which can conduct service cluster head generation, service cluster composition, and service discovery. The proposed method utilizes a superior data preprocessing method. Using simulation experiments,the efficiency and precision of Web service discovery are illustrated. Finally, the application value of the approach on real Web service is discussed.展开更多
Objective:To study the effect of ulinastatin combined with antibiotics on systemic inflammatory response and stress hormone secretion in patients with acute severe pneumonia. Methods: A total of 78 patients with acute...Objective:To study the effect of ulinastatin combined with antibiotics on systemic inflammatory response and stress hormone secretion in patients with acute severe pneumonia. Methods: A total of 78 patients with acute severe pneumonia in our hospital between December 2014 and January 2017 were randomly divided into routine group and ulinastatin group, each with 39 cases. Routine group were treated with conventional antibiotics, ulinastatin group were treated with ulinastatin and antibiotics, and the treatments lasted for 2 weeks. The differences of serum acute phase proteins, inflammatory factors and stress hormones were compared between the two groups before and after treatment.Results: Before treatment, there were no significant differences in serum contents of acute phase proteins, inflammatory factors or stress hormones between the two groups. After 2 weeks of treatment, serum acute phase protein prealbumin (PA) content in ulinastatin group was higher than that in routine group whereas C-reactive protein (CRP) content was lower than that in routine group;serum inflammatory cytokines interleukin-1β (IL-1β), interleukin-2 (IL-2) and interleukin-6 (IL-6) contents in ulinastatin group were lower than those in routine group;serum stress hormones NE, Cor and AngII contents in ulinastatin group were lower than those in routine group. Conclusion:Ulinastatin combined with antibiotics for acute severe pneumonia can inhibit the systemic inflammatory response and stress response.展开更多
Addition of H2O2 has been employed to repress bromate formation during ozonation of bromide-containing source water. However, the addition of H2O2 will change the oxidation pathways of organic compounds due to the gen...Addition of H2O2 has been employed to repress bromate formation during ozonation of bromide-containing source water. However, the addition of H2O2 will change the oxidation pathways of organic compounds due to the generation of abundant hydroxyl radicals, which could affect the removal efficacy of trihalomethane precursors via the combination of ozone and biological activated carbon （O3-BAC）. In this study, we evaluated the effects of H2O2 addition on bromate formation and trihalomethane formation potential （THMFP） reduction during treatment of bromide-containing （97.6-129.1 μg/L） source water by the O3-BAC process. At an ozone dose of 4.2 mg/L, an H2O2/O3 （g/g） ratio of over 1.0 was required to maintain the bromate concentration below 10.0 μg/L, while a much lower H2O2/O3 ratio was sufficient for a lower ozone dose. An H2O2/O3 （g/g） ratio below 0.3 should be avoided since the bromate concentration will increase with increasing H2O2 dose below this ratio. However, the addition of H2O2 at an ozone dose of 3.2 mg/L and an H2O2/O3 ratio of 1.0 resulted in a 43% decrease in THMFP removal when compared with the O3-BAC process. The optimum H2O2/O3 （g/g） ratio for balancing bromate and trihalomethane control was about 0.7-1.0. Fractionation of organic materials showed that the addition of H2O2 decreased the removal efficacy of the hydrophilic matter fraction of DOC by ozonation and increased the reactivity of the hydrophobic fractions during formation of trihalomethane, which may be the two main reasons responsible for the decrease in THMFP reduction efficacy. Overall, this study clearly demonstrated that it is necessary to balance bromate reduction and THMFP control when adopting an H2O2 addition strategy.展开更多
基金supported by the National Natural Science Foundation of China,No.81202941the Key Project Foundation of Oversea Visiting and Research for the Excellent Young and Middle-aged Faculties in Universities of Anhui Province in China,No.gxfx ZD2016119the Key Project Foundation of Natural Science Research in Universities of Anhui Province in China,No.KJ2016A406
文摘Genistein is effective against amyloid-β toxicity, but the underlying mechanisms are unclear. We hypothesized that genistein may protect neurons by inhibiting the mitochondrial apoptotic pathway, and thereby play a role in the prevention of Alzheimer＇s disease. A rat model of Alzheimer＇s disease was established by intraperitoneal injection of D-galactose and intracerebral injection of amyloid-β peptide （25-35）. In the genistein treatment groups, a 7-day pretreatment with genistein （10, 30, 90 mg/kg） was given prior to establishing Alzheimer＇s disease model, for 49 consecutive days. Terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling assay demonstrated a reduction in apoptosis in the hippocampus of rats treated with genistein. Western blot analysis showed that expression levels of capase-3, Bax and cytochrome c were decreased compared with the model group. Furthermore, immunohistochemical staining revealed reductions in cytochrome c and Bax immunoreactivity in these rats. Morris water maze revealed a substantial shortening of escape latency by genistein in Alzheimer＇s disease rats. These findings suggest that genistein decreases neuronal loss in the hippocampus, and improves learning and memory ability. The neuroprotective effects of genistein are associated with the inhibition of the mitochondrial apoptotic pathway, as shown by its ability to reduce levels of caspase-3, Bax and cytochrome c.
基金supported by the National Natural Science Foundation of China(61170078,61472228)the Science and Technology Development Plan of Shandong Province(2012G0020120)the Science and Technology Innovation Project of Shandong University of Science and Technology(SDKDYC17022)
文摘In recent years, the number of Web services has increased significantly. Web service discovery has drawn much attention with the development of Web service applications and big data analysis. Under this circumstance, traditional Web service discovery strategies cannot adequately meet high user requirements due to the efficiency and precision of service discovery is low. In order to improve the accuracy and efficiency of service discovery, a user requirement oriented Web service discovery approach based on Petri nets is proposed in this study. A data preprocessing strategy of Web service is first designed. Then, a service clustering method is proposed based on Petri nets, which can conduct service cluster head generation, service cluster composition, and service discovery. The proposed method utilizes a superior data preprocessing method. Using simulation experiments,the efficiency and precision of Web service discovery are illustrated. Finally, the application value of the approach on real Web service is discussed.
文摘Objective:To study the effect of ulinastatin combined with antibiotics on systemic inflammatory response and stress hormone secretion in patients with acute severe pneumonia. Methods: A total of 78 patients with acute severe pneumonia in our hospital between December 2014 and January 2017 were randomly divided into routine group and ulinastatin group, each with 39 cases. Routine group were treated with conventional antibiotics, ulinastatin group were treated with ulinastatin and antibiotics, and the treatments lasted for 2 weeks. The differences of serum acute phase proteins, inflammatory factors and stress hormones were compared between the two groups before and after treatment.Results: Before treatment, there were no significant differences in serum contents of acute phase proteins, inflammatory factors or stress hormones between the two groups. After 2 weeks of treatment, serum acute phase protein prealbumin (PA) content in ulinastatin group was higher than that in routine group whereas C-reactive protein (CRP) content was lower than that in routine group;serum inflammatory cytokines interleukin-1β (IL-1β), interleukin-2 (IL-2) and interleukin-6 (IL-6) contents in ulinastatin group were lower than those in routine group;serum stress hormones NE, Cor and AngII contents in ulinastatin group were lower than those in routine group. Conclusion:Ulinastatin combined with antibiotics for acute severe pneumonia can inhibit the systemic inflammatory response and stress response.
基金supported by the National Natural Science Foundation of China (No. 50938007)the Funds for Major Science and Technology Program for Water Pollution Control and Treatment (No. 2012ZX07403-00202)the Special Co-construction Project of the Beijing Municipal Commission of Education
文摘Addition of H2O2 has been employed to repress bromate formation during ozonation of bromide-containing source water. However, the addition of H2O2 will change the oxidation pathways of organic compounds due to the generation of abundant hydroxyl radicals, which could affect the removal efficacy of trihalomethane precursors via the combination of ozone and biological activated carbon （O3-BAC）. In this study, we evaluated the effects of H2O2 addition on bromate formation and trihalomethane formation potential （THMFP） reduction during treatment of bromide-containing （97.6-129.1 μg/L） source water by the O3-BAC process. At an ozone dose of 4.2 mg/L, an H2O2/O3 （g/g） ratio of over 1.0 was required to maintain the bromate concentration below 10.0 μg/L, while a much lower H2O2/O3 ratio was sufficient for a lower ozone dose. An H2O2/O3 （g/g） ratio below 0.3 should be avoided since the bromate concentration will increase with increasing H2O2 dose below this ratio. However, the addition of H2O2 at an ozone dose of 3.2 mg/L and an H2O2/O3 ratio of 1.0 resulted in a 43% decrease in THMFP removal when compared with the O3-BAC process. The optimum H2O2/O3 （g/g） ratio for balancing bromate and trihalomethane control was about 0.7-1.0. Fractionation of organic materials showed that the addition of H2O2 decreased the removal efficacy of the hydrophilic matter fraction of DOC by ozonation and increased the reactivity of the hydrophobic fractions during formation of trihalomethane, which may be the two main reasons responsible for the decrease in THMFP reduction efficacy. Overall, this study clearly demonstrated that it is necessary to balance bromate reduction and THMFP control when adopting an H2O2 addition strategy.
