AIM:To evaluate whether a novel tyrosine kinase inhibitor nintedanib could inhibit basic fibroblast growth factor(bFGF)and vascular endothelial growth factor(VEGF)simultaneously for retinal vascular disease in vivo.ME...AIM:To evaluate whether a novel tyrosine kinase inhibitor nintedanib could inhibit basic fibroblast growth factor(bFGF)and vascular endothelial growth factor(VEGF)simultaneously for retinal vascular disease in vivo.METHODS:After a laser induced rabbit retinal vein occlusion(RVO)model was made,0.5 mg of nintedanib was injected intravitreally in the left eye on the third day while the right eye was as a control.Intracameral samples were taken on the day before laser treatment and days 1,3,7,14,21,and 28 after treatment.Enzyme-linked immunosorbent assay(ELISA)was used to test the bFGF and VEGF-A concentrations in the aqueous humor.RESULTS:Both bFGF and VEGF-A rose significantly on the third day after laser treatment in both eyes.In the control eye the bFGF concentration peaked on the 14th day while the VEGF-A concentration dropped rapidly soon after the third day.After nintadanib injection in the study eye,both bFGF and VEGF-A showed a significant reduction on the 4th day(7th day after laser treatment)when compared to the control eye,and kept on low level in the following several weeks.CONCLUSION:Intravitreal injection of nintedanib can inhibit the expression of bFGF and VEGF in the process of RVO model to a certain extent,which is expected to become a new method for the treatment of retinal vascular diseases or fibrotic diseases.展开更多
With the rapid development of computer technology,the application of artificial intelligence(AI)to ophthalmology has gained prominence in modern medicine.As modern optometry is closely related to ophthalmology,AI rese...With the rapid development of computer technology,the application of artificial intelligence(AI)to ophthalmology has gained prominence in modern medicine.As modern optometry is closely related to ophthalmology,AI research on optometry has also increased.This review summarizes current AI research and technologies used for diagnosis in optometry,related to myopia,strabismus,amblyopia,optical glasses,contact lenses,and other aspects.The aim is to identify mature AI models that are suitable for research on optometry and potential algorithms that may be used in future clinical practice.展开更多
AIM:To evaluate the clinical factors related to chronic rhegmatogenous retinal detachment (RRD).· METHODS:A retrospective case-control study.A total of 103 consecutive patients (103 eyes) with primary RRD were st...AIM:To evaluate the clinical factors related to chronic rhegmatogenous retinal detachment (RRD).· METHODS:A retrospective case-control study.A total of 103 consecutive patients (103 eyes) with primary RRD were studied to evaluate the clinical factors related to chronic RRD.· RESULTS:Chi-square test was used to sift out the following associated factors with chronic RRD:younger patients(P =0.0028),better preoperative best corrected visual acuity (BCVA,P =0.0316),atrophic retinal break (P <0.0001),inferior retinal break (P <0.0001),smaller break (P =0.0005);then the independent risk factors related to chronic RRD was determined by stepwise logistic regression analysis as following:atrophic retinal break (odds ratio (OR)=7.997,P =0.007),inferior retinal break (OR=14.127,P <0.0001) and better preoperative BCVA (OR=1.636 P <0.0722).· CONCLUSION:Atrophic retinal break,inferior retinal break and better preoperative BCVA are the independent risk factors related to chronic RRD.展开更多
AIM:To observe whether silicone oil(SO)tamponade could decrease macular perfusion after retinal detachment repair.METHODS:A prospective observational case-control study.Patients diagnosed with primary macular off rheg...AIM:To observe whether silicone oil(SO)tamponade could decrease macular perfusion after retinal detachment repair.METHODS:A prospective observational case-control study.Patients diagnosed with primary macular off rhegmatogenous retinal detachment undergoing successful retinal repair surgery with vitrectomy were strictly selected.Optical coherence tomography angiography findings were compared between SO and air tamponade groups.Two postoperative visiting points were set(1 and 3 mo).RESULTS:Totally 29 patients(29 eyes)were enrolled.Twenty cases had SO tamponade while 9 cases were with air tamponade.At the first visiting point,superficial parafoveal vessel density(PFSVD)significantly decreased in the SO group(P=0.0403),especially in the superior quadrant or superior-hemi area(P=0.0089,0.0426,respectively).Parafoveal deep vessel density(PFDVD)had no difference between the two groups.At the second visiting point,all quadrants of PFSVD reduced significantly in the SO group(P=0.0256,0.0001,0.0031,<0.0001 in temporal,superior,nasal,and inferior area,respectively),but PFDVD remained no different.In the air group,all areas of PFSVD showed significantly improving from the first visit to the second one(P=0.0324,0.0001,0.0371,0.0026,in temporal,superior,nasal,and inferior area,respectively);however,almost all quadrants of PFDVD showed no changes during this period.In the SO group,both PFSVD and PFDVD showed no obvious changes between the two visiting points.Besides,parafoveal full retinal thickness in the SO group reduced significantly at both visiting points over the air tamponade,while the foveal avascular zone area showed no difference in the two groups.CONCLUSION:After retinal detachment surgery with vitrectomy and SO tamponade,superficial macular perfusion and full retinal thickness could decrease obviously when compared to air tamponade.This reduction process could persist throughout the tamponade period.展开更多
Background Many studies have demonstrated the effectiveness of Screening,Brief Intervention and Referral to Treatment(SBIRT)in addressing substance use problem.However,owing to the shortage of counsellors,it has not b...Background Many studies have demonstrated the effectiveness of Screening,Brief Intervention and Referral to Treatment(SBIRT)in addressing substance use problem.However,owing to the shortage of counsellors,it has not been widely used in China.With the development of smart medicine,we developed a web-based electronic SBIRT(E-SBIRT)program and explored the effectiveness of E-SBIRT in reducing substance use in China.Methods A randomised controlled trial will be conducted in primary healthcare institutions.Four primary healthcare institutions will be selected and randomly divided into an intervention group and a control group(each institution will recruit 60 participants,and in total,240 participants will be recruited).The control group will get a pamphlet of drug abuse prevention,and the intervention group will get the E-SBIRT intervention and the pamphlet.Both groups will receive baseline and follow-up assessment at 1 and 3 months after the intervention.The primary outcome is the change in scores on the Alcohol,Smoking and Substance Use Involvement Screening Test,and the secondary outcomes include changes in motivation,depression,anxiety,positive/negative emotion,self-esteem,addiction knowledge and addiction severity index.Conclusions If the‘E-SBIRT’program is found to be effective,it will be an accessible,affordable and widely implementable intervention to help participants at moderate risk of substance use to reduce their consumption.The potential benefit is to provide early intervention to high-risk patients in time and reduce the harmful consequences to individuals and society.展开更多
In order to understand the stress corrosion behavior of super-high strength aluminum alloys by spray forming, different aluminum alloys by different heat treatment was made. The results showed that the alloy with peak...In order to understand the stress corrosion behavior of super-high strength aluminum alloys by spray forming, different aluminum alloys by different heat treatment was made. The results showed that the alloy with peak aging has the most sensitive stress corrosion cracking, the crack can even be seen using eyes;the alloys with two step aging were better than one step aging alloys, the alloys has not been found stress corrosion cracking.展开更多
Sphingolipids, including sphingosine-1-phosphate (S1P), have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed fun...Sphingolipids, including sphingosine-1-phosphate (S1P), have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice (Oryza sativa) S1P lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid (ABA), and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPL1-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPL1-overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.展开更多
BaSO_(4) nanoparticles as important functional materials have attracted considerable research interests,due to their X-rays barrier and absorption properties.However,most of BaSO_(4) nanoparticles prepared by traditio...BaSO_(4) nanoparticles as important functional materials have attracted considerable research interests,due to their X-rays barrier and absorption properties.However,most of BaSO_(4) nanoparticles prepared by traditional technology are nanopowders with broad size distribution and poor dispersibility,which may greatly limit their applications.To the best of our knowledge,the synthesis of transparent BaSO_(4) nanodispersions was rarely reported.Here,we firstly present a novel and efficient method to prepare transparent and stable BaSO_(4) nanodispersions with a relatively small particle size around 10 to 17 nm using a precipitation method in a rotating packed bed(RPB),followed by a modification treatment using stearic acid.Compared with the BaSO_(4) prepared in a traditional stirred tank,the product prepared using an RPB has much smaller particle size and narrower size distribution.More importantly,by using RPB,the reaction time can be significantly decreased from 20 min to 18 s.Furthermore,the transparent BaSO_(4)-polyvinyl butyral nanocomposite films with good X-ray shielding performance can be easily fabricated.We believe that the stable BaSO_(4) nanodispersions may have a wide range of applications for transparent composite materials and coatings with X-ray shielding performance for future research.展开更多
Cryopreservation of rare testicular-retrieved spermatozoa for intracytoplasmic sperm injection(ICSI)in patients with severe oligozoospermia and azoospermia remains a major challenge in clinical practice.This study eva...Cryopreservation of rare testicular-retrieved spermatozoa for intracytoplasmic sperm injection(ICSI)in patients with severe oligozoospermia and azoospermia remains a major challenge in clinical practice.This study evaluated the Cryopiece system as a potential technique to cryopreserve rare human spermatozoa for ICSI.Small numbers of ejaculated(24 patients)and testicular(13 patients)spermatozoa were cryopreserved using the Cryopiece system.The total number of recovered spermatozoa and motility were assessed after thawing.Thirty-seven couples underwent ICSI using spermatozoa cryopreserved by the Cryopiece system,and ICSI outcomes(rates of fertilization,embryo cleavage,and clinical pregnancy)were evaluated.The average sperm post-thaw retrieval rate was 79.1%,and motility was 29.7%.Ejaculated spermatozoa had a higher post-thaw motility(32.5%)than testicular spermatozoa(21.8%;P=0.005).ICSI achieved a fertilization rate of 61.9%,embryo cleavage rate of 84.6%,and clinical pregnancy rate of 43.3%.The ICSI outcomes in the ejaculated and testicular frozen-thawed spermatozoa were similar.Assisted oocyte activation(AOA)after ICSI with motile(72.1%)or immotile(71.9%)spermatozoa resulted in a significantly higher fertilization rate than that when using motile spermatozoa without AOA(52.0%;P=0.005).However,AOA did not enhance the clinical pregnancy rate(55.6%or 40.0%vs 35.3%;P=0.703).The Cryopiece system is simple and useful for the cryopreservation of small numbers of ejaculated or testicular spermatozoa for ICSI in patients with severe oligozoospermia or nonobstructive azoospermia.展开更多
基金Supported by Medical Health Science and Technology Project of Zhejiang Province(No.2020KY654).
文摘AIM:To evaluate whether a novel tyrosine kinase inhibitor nintedanib could inhibit basic fibroblast growth factor(bFGF)and vascular endothelial growth factor(VEGF)simultaneously for retinal vascular disease in vivo.METHODS:After a laser induced rabbit retinal vein occlusion(RVO)model was made,0.5 mg of nintedanib was injected intravitreally in the left eye on the third day while the right eye was as a control.Intracameral samples were taken on the day before laser treatment and days 1,3,7,14,21,and 28 after treatment.Enzyme-linked immunosorbent assay(ELISA)was used to test the bFGF and VEGF-A concentrations in the aqueous humor.RESULTS:Both bFGF and VEGF-A rose significantly on the third day after laser treatment in both eyes.In the control eye the bFGF concentration peaked on the 14th day while the VEGF-A concentration dropped rapidly soon after the third day.After nintadanib injection in the study eye,both bFGF and VEGF-A showed a significant reduction on the 4th day(7th day after laser treatment)when compared to the control eye,and kept on low level in the following several weeks.CONCLUSION:Intravitreal injection of nintedanib can inhibit the expression of bFGF and VEGF in the process of RVO model to a certain extent,which is expected to become a new method for the treatment of retinal vascular diseases or fibrotic diseases.
基金Supported by the Zhejiang Provincial Medical and Health Science Technology Program of Health Commission(No.2022PY074,No.2022KY217)the Scientific Research Fund of Zhejiang Provincial Education Department(No.Y202147994).
文摘With the rapid development of computer technology,the application of artificial intelligence(AI)to ophthalmology has gained prominence in modern medicine.As modern optometry is closely related to ophthalmology,AI research on optometry has also increased.This review summarizes current AI research and technologies used for diagnosis in optometry,related to myopia,strabismus,amblyopia,optical glasses,contact lenses,and other aspects.The aim is to identify mature AI models that are suitable for research on optometry and potential algorithms that may be used in future clinical practice.
文摘AIM:To evaluate the clinical factors related to chronic rhegmatogenous retinal detachment (RRD).· METHODS:A retrospective case-control study.A total of 103 consecutive patients (103 eyes) with primary RRD were studied to evaluate the clinical factors related to chronic RRD.· RESULTS:Chi-square test was used to sift out the following associated factors with chronic RRD:younger patients(P =0.0028),better preoperative best corrected visual acuity (BCVA,P =0.0316),atrophic retinal break (P <0.0001),inferior retinal break (P <0.0001),smaller break (P =0.0005);then the independent risk factors related to chronic RRD was determined by stepwise logistic regression analysis as following:atrophic retinal break (odds ratio (OR)=7.997,P =0.007),inferior retinal break (OR=14.127,P <0.0001) and better preoperative BCVA (OR=1.636 P <0.0722).· CONCLUSION:Atrophic retinal break,inferior retinal break and better preoperative BCVA are the independent risk factors related to chronic RRD.
基金The authors would like to thank National Natural Science Foundation of China （No. 20776122）；Hebei Natural Science Foundation （No. B2006000191）；State Key Laboratory of Fine Chemicals, Dalian University of Technology （No. KF0502） for providing the financial support for this project.
基金Wenzhou Municipal Science and Technology Bureau(No.Y20180730)。
文摘AIM:To observe whether silicone oil(SO)tamponade could decrease macular perfusion after retinal detachment repair.METHODS:A prospective observational case-control study.Patients diagnosed with primary macular off rhegmatogenous retinal detachment undergoing successful retinal repair surgery with vitrectomy were strictly selected.Optical coherence tomography angiography findings were compared between SO and air tamponade groups.Two postoperative visiting points were set(1 and 3 mo).RESULTS:Totally 29 patients(29 eyes)were enrolled.Twenty cases had SO tamponade while 9 cases were with air tamponade.At the first visiting point,superficial parafoveal vessel density(PFSVD)significantly decreased in the SO group(P=0.0403),especially in the superior quadrant or superior-hemi area(P=0.0089,0.0426,respectively).Parafoveal deep vessel density(PFDVD)had no difference between the two groups.At the second visiting point,all quadrants of PFSVD reduced significantly in the SO group(P=0.0256,0.0001,0.0031,<0.0001 in temporal,superior,nasal,and inferior area,respectively),but PFDVD remained no different.In the air group,all areas of PFSVD showed significantly improving from the first visit to the second one(P=0.0324,0.0001,0.0371,0.0026,in temporal,superior,nasal,and inferior area,respectively);however,almost all quadrants of PFDVD showed no changes during this period.In the SO group,both PFSVD and PFDVD showed no obvious changes between the two visiting points.Besides,parafoveal full retinal thickness in the SO group reduced significantly at both visiting points over the air tamponade,while the foveal avascular zone area showed no difference in the two groups.CONCLUSION:After retinal detachment surgery with vitrectomy and SO tamponade,superficial macular perfusion and full retinal thickness could decrease obviously when compared to air tamponade.This reduction process could persist throughout the tamponade period.
基金This work was supported by the Shanghai Mental Health Center(SMHC)Clinical Research Center Project(CRC2017YB04).
文摘Background Many studies have demonstrated the effectiveness of Screening,Brief Intervention and Referral to Treatment(SBIRT)in addressing substance use problem.However,owing to the shortage of counsellors,it has not been widely used in China.With the development of smart medicine,we developed a web-based electronic SBIRT(E-SBIRT)program and explored the effectiveness of E-SBIRT in reducing substance use in China.Methods A randomised controlled trial will be conducted in primary healthcare institutions.Four primary healthcare institutions will be selected and randomly divided into an intervention group and a control group(each institution will recruit 60 participants,and in total,240 participants will be recruited).The control group will get a pamphlet of drug abuse prevention,and the intervention group will get the E-SBIRT intervention and the pamphlet.Both groups will receive baseline and follow-up assessment at 1 and 3 months after the intervention.The primary outcome is the change in scores on the Alcohol,Smoking and Substance Use Involvement Screening Test,and the secondary outcomes include changes in motivation,depression,anxiety,positive/negative emotion,self-esteem,addiction knowledge and addiction severity index.Conclusions If the‘E-SBIRT’program is found to be effective,it will be an accessible,affordable and widely implementable intervention to help participants at moderate risk of substance use to reduce their consumption.The potential benefit is to provide early intervention to high-risk patients in time and reduce the harmful consequences to individuals and society.
文摘In order to understand the stress corrosion behavior of super-high strength aluminum alloys by spray forming, different aluminum alloys by different heat treatment was made. The results showed that the alloy with peak aging has the most sensitive stress corrosion cracking, the crack can even be seen using eyes;the alloys with two step aging were better than one step aging alloys, the alloys has not been found stress corrosion cracking.
基金supported by the National Key Project for Research on Transgenic Plants (2009ZX08001-017B and 2011ZX08009-003-001)the National Natural Science Foundation of China (No. 30971880)+1 种基金the National High-tech R&D Program of China (No. 2 012AA101504)the Program for Changjiang Scholars and Innovative Research Team in the University of the Ministry of Education of China (No. IRT0943)
文摘Sphingolipids, including sphingosine-1-phosphate (S1P), have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice (Oryza sativa) S1P lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid (ABA), and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPL1-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPL1-overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.
基金This work was financially supported by the National Key Research and Development Program of China(Grant No.2016YFA0201701/2016YFA0201700)the National Natural Science Foundation of China(Grant No.21878015).
文摘BaSO_(4) nanoparticles as important functional materials have attracted considerable research interests,due to their X-rays barrier and absorption properties.However,most of BaSO_(4) nanoparticles prepared by traditional technology are nanopowders with broad size distribution and poor dispersibility,which may greatly limit their applications.To the best of our knowledge,the synthesis of transparent BaSO_(4) nanodispersions was rarely reported.Here,we firstly present a novel and efficient method to prepare transparent and stable BaSO_(4) nanodispersions with a relatively small particle size around 10 to 17 nm using a precipitation method in a rotating packed bed(RPB),followed by a modification treatment using stearic acid.Compared with the BaSO_(4) prepared in a traditional stirred tank,the product prepared using an RPB has much smaller particle size and narrower size distribution.More importantly,by using RPB,the reaction time can be significantly decreased from 20 min to 18 s.Furthermore,the transparent BaSO_(4)-polyvinyl butyral nanocomposite films with good X-ray shielding performance can be easily fabricated.We believe that the stable BaSO_(4) nanodispersions may have a wide range of applications for transparent composite materials and coatings with X-ray shielding performance for future research.
基金supported by the National Key R&D Program of China(No.2017YFC1002003)the National Natural Science Foundation of China(No.81771637 and No.81571488)+2 种基金Shanghai Collaborative Innovation Center for Translational Medicine(No.TM202015 to ZJZ)the China Postdoctoral Science Foundation(No.2019M661521)the Key Project of Research and Development of Ningxia Hui Autonomous Region of China(No.2020BFH02002).
文摘Cryopreservation of rare testicular-retrieved spermatozoa for intracytoplasmic sperm injection(ICSI)in patients with severe oligozoospermia and azoospermia remains a major challenge in clinical practice.This study evaluated the Cryopiece system as a potential technique to cryopreserve rare human spermatozoa for ICSI.Small numbers of ejaculated(24 patients)and testicular(13 patients)spermatozoa were cryopreserved using the Cryopiece system.The total number of recovered spermatozoa and motility were assessed after thawing.Thirty-seven couples underwent ICSI using spermatozoa cryopreserved by the Cryopiece system,and ICSI outcomes(rates of fertilization,embryo cleavage,and clinical pregnancy)were evaluated.The average sperm post-thaw retrieval rate was 79.1%,and motility was 29.7%.Ejaculated spermatozoa had a higher post-thaw motility(32.5%)than testicular spermatozoa(21.8%;P=0.005).ICSI achieved a fertilization rate of 61.9%,embryo cleavage rate of 84.6%,and clinical pregnancy rate of 43.3%.The ICSI outcomes in the ejaculated and testicular frozen-thawed spermatozoa were similar.Assisted oocyte activation(AOA)after ICSI with motile(72.1%)or immotile(71.9%)spermatozoa resulted in a significantly higher fertilization rate than that when using motile spermatozoa without AOA(52.0%;P=0.005).However,AOA did not enhance the clinical pregnancy rate(55.6%or 40.0%vs 35.3%;P=0.703).The Cryopiece system is simple and useful for the cryopreservation of small numbers of ejaculated or testicular spermatozoa for ICSI in patients with severe oligozoospermia or nonobstructive azoospermia.
