AIM: To study the effect of type 1 Na+/H+ exchanger (NHE1 ) antisense human gene transfection on the biological behavior of gastric carcinoma cell line SGC-7901. METHODS: Antisense NHE1 eukaryotic expression on vector...AIM: To study the effect of type 1 Na+/H+ exchanger (NHE1 ) antisense human gene transfection on the biological behavior of gastric carcinoma cell line SGC-7901. METHODS: Antisense NHE1 eukaryotic expression on vector pcDNA3.1 was constructed by recombinant DNA technique and transfected into gastric carcinoma cell line SGC-7901 with DOTAP liposome transfection method. Morphological changes of cells were observed with optic and electron microscopes. Changes in cell proliferative capacity, apoptosis, intracellular pH (pHi), cell cycle, clone formation in two-layer soft agar, and tumorigenicity in nude mice were examined. RESULTS: Antisense eukaryotic expressing vectors were successfully constructed and transfected into SGC-7901. The transfectant obtained named 7901 -antisense (7901-AS) stablely produced antisense NHE1. There was a significant difference between the pHi of 7901-AS cells (6.77 ± 0.05) and that of 7901-zeo cells and SGC-7901 cells (7.24 ± 0.03 and 7.26 ± 0.03, P < 0.01). Compared with SGC-7901 and 7901-zeo cells, 7901-AS cells mostly showed cell proliferation inhibition, G1/G0 phase arrest, increased cell apoptotic rate, recovery of contact inhibition, and density contact. The tumorigenicity in nude mice and cloning efficiency in the two-layer soft agar were clearly inhibited. CONCLUSION: NHE1 antisense gene significantly restrains the malignant behavior of human gastric carcinoma cells, suppresses cell growth and induces cell apoptosis, and partially reverses the malignant phenotypes of SGC-7901 . These results suggest a potential role for human tumor gene therapy.展开更多
BACKGROUND:Earthquakes,floods,droughts,storms,mudslides,landslides,and forest wild fires are serious threats to human lives and properties.The present study aimed to study the environmental characteristics and pathoge...BACKGROUND:Earthquakes,floods,droughts,storms,mudslides,landslides,and forest wild fires are serious threats to human lives and properties.The present study aimed to study the environmental characteristics and pathogenic traits,recapitulate experiences,and augment applications of medical reliefs in tropical regions.METHODS:Analysis was made on work and projects of emergency medical rescue,based on information and data collected from 3 emergency medical rescue missions of China International Search and Rescue Team to overseas earthquakes and tsunamis aftermaths in tropical disaster regions — Indonesia-Aceh,Indonesia-Yogyakarta,and Haiti-Port au Prince.RESULTS:Shock,infection and heat stroke were frequently encountered in addition to outbreaks of infectious diseases,skin diseases,and diarrhea during post-disaster emergency medical rescue in tropical regions.CONCLUSIONS:High temperature,high humidity,and proliferation of microorganisms and parasites are the characteristics of tropical climate that impose strict requirements on the preparation of rescue work including selective team members suitable for a particular rescue mission and the provisioning of medical equipment and life support materials.The overseas rescue mission itself needs a scientific,efficient,simple workflow for providing efficient emergency medical assistance.Since shock and infection are major tasks in post-disaster treatment of severely injured victims in tropical regions,the prevention and diagnosis of hyperthermia,insect-borne infectious diseases,tropic skin diseases,infectious diarrhea,and pest harms of disaster victims and rescue team staff should be emphasized during the rescue operations.展开更多
Objective：To evaluate the technical characteristics and application of mass casualty incident （MCI） primary triage （PT） methods applied in China.Data Sources：Chinese literature was searched by Chinese Academic J...Objective：To evaluate the technical characteristics and application of mass casualty incident （MCI） primary triage （PT） methods applied in China.Data Sources：Chinese literature was searched by Chinese Academic Journal Network Publishing Database （founded in June 2014）.The English literature was searched by PubMed （MEDLINE） （1950 to June 2014）.We also searched Official Websites of Chinese Central Government＇s （http：//www.gov.cn/）,National Health and Family Planning Commission of China （http：//www.nhfpc.gov.cn/）,and China Earthquake Information （http：//www.csi.ac.cn/）.Study Selection：We included studies associated with mass casualty events related to China,the PT applied in China,guidelines and standards,and application and development of the carding PT method in China.Results：From 3976 potentially relevant articles,22 met the inclusion criteria,20 Chinese,and 2 English.These articles included 13 case reports,3 retrospective analyses of MCI,two methods introductions,three national or sectoral criteria,and one simulated field testing and validation.There were a total of 19 kinds ofMCI PT methods that have been reported in China from 1950 to 2014.In addition,there were 15 kinds of PT methods reported in the literature from the instance of the application.Conclusions：The national and sectoral current triage criteria are developed mainly for earthquake relief.Classification is not clear.Vague criteria （especially between moderate and severe injuries） operability are not practical.There are no triage methods and research for children and special populations.There is no data and evidence supported triage method.We should revise our existing classification and criteria so it is clearer and easier to be grasped in order to build a real,practical,and efficient PT method.展开更多
Background：Inflammation plays a pivotal role in the formation and progression of ischemic stroke.Recently,more and more epidemiological studies have focused on the association between C-reactive protein （CRP）-717A ...Background：Inflammation plays a pivotal role in the formation and progression of ischemic stroke.Recently,more and more epidemiological studies have focused on the association between C-reactive protein （CRP）-717A 〉 G and-286C 〉 T 〉 A genetic polymorphisms and ischemic stroke.However,the findings of these researches are not conclusive.Methods：We performed a meta-analysis to determine whether these two polymorphisms are associated with the risk of ischemic stroke.Eligible studies were identified from the database of PubMed,Medline,Embase,Web of Science,CNKI,Weipu,and Wanfang.We calculated odds ratios （ORs） with 95% confidence intervals （CIs） to assess the strength of the association.Results：Four articles were included in our study,including 1926 cases and 2678 controls for-717A 〉 G polymorphism,652 cases and 1103 controls for-286C 〉 T 〉 A polymorphism.The results of meta-analysis showed that single nucleotide polymorphism （SNP）-717A 〉 G was not significantly associated with the risk of ischemic stroke （GG vs.AA,OR =1.12,95% CI =0.83-1.50,P =0.207;GG ＋ GA vs.AA,OR =1.04,95% CI =0.93-1.17,P =0.533;GG vs.GA ＋ AA,OR =1.10,95% CI =0.82-1.47,P =0.220）.Meta-analysis of SNP-286C 〉 T 〉 A also demonstrated no statistical evidence of a significant association with the risk of ischemic stroke （AA vs.CC,OR =0.86,95% CI =0.59-1.25,P =0.348;AA vs.CC,OR =0.92,95% CI =0.80-1.06,P =0.609;AA vs.CC,OR =0.89,95% CI =0.62-1.30,P =0.374）.Conclusions：This meta-analysis demonstrated little evidence to support a role of CRP gene-717A 〉 G,-286C 〉 T 〉 A polymorphisms in ischemic stroke predisposition.However,to draw comprehensive and more reliable conclusions,further larger studies are needed to validate the association between CRP gene polymorphisms and ischemic stroke in various ethnic groups.展开更多
基金The grant from the Science Foundation of Chinese People’s Armed Police Forces, No. WZ200415 and No. WZ200511
文摘AIM: To study the effect of type 1 Na+/H+ exchanger (NHE1 ) antisense human gene transfection on the biological behavior of gastric carcinoma cell line SGC-7901. METHODS: Antisense NHE1 eukaryotic expression on vector pcDNA3.1 was constructed by recombinant DNA technique and transfected into gastric carcinoma cell line SGC-7901 with DOTAP liposome transfection method. Morphological changes of cells were observed with optic and electron microscopes. Changes in cell proliferative capacity, apoptosis, intracellular pH (pHi), cell cycle, clone formation in two-layer soft agar, and tumorigenicity in nude mice were examined. RESULTS: Antisense eukaryotic expressing vectors were successfully constructed and transfected into SGC-7901. The transfectant obtained named 7901 -antisense (7901-AS) stablely produced antisense NHE1. There was a significant difference between the pHi of 7901-AS cells (6.77 ± 0.05) and that of 7901-zeo cells and SGC-7901 cells (7.24 ± 0.03 and 7.26 ± 0.03, P < 0.01). Compared with SGC-7901 and 7901-zeo cells, 7901-AS cells mostly showed cell proliferation inhibition, G1/G0 phase arrest, increased cell apoptotic rate, recovery of contact inhibition, and density contact. The tumorigenicity in nude mice and cloning efficiency in the two-layer soft agar were clearly inhibited. CONCLUSION: NHE1 antisense gene significantly restrains the malignant behavior of human gastric carcinoma cells, suppresses cell growth and induces cell apoptosis, and partially reverses the malignant phenotypes of SGC-7901 . These results suggest a potential role for human tumor gene therapy.
基金supported by a grant from Capital Medical Development Scientific Research Fund(2009-1029)
文摘BACKGROUND:Earthquakes,floods,droughts,storms,mudslides,landslides,and forest wild fires are serious threats to human lives and properties.The present study aimed to study the environmental characteristics and pathogenic traits,recapitulate experiences,and augment applications of medical reliefs in tropical regions.METHODS:Analysis was made on work and projects of emergency medical rescue,based on information and data collected from 3 emergency medical rescue missions of China International Search and Rescue Team to overseas earthquakes and tsunamis aftermaths in tropical disaster regions — Indonesia-Aceh,Indonesia-Yogyakarta,and Haiti-Port au Prince.RESULTS:Shock,infection and heat stroke were frequently encountered in addition to outbreaks of infectious diseases,skin diseases,and diarrhea during post-disaster emergency medical rescue in tropical regions.CONCLUSIONS:High temperature,high humidity,and proliferation of microorganisms and parasites are the characteristics of tropical climate that impose strict requirements on the preparation of rescue work including selective team members suitable for a particular rescue mission and the provisioning of medical equipment and life support materials.The overseas rescue mission itself needs a scientific,efficient,simple workflow for providing efficient emergency medical assistance.Since shock and infection are major tasks in post-disaster treatment of severely injured victims in tropical regions,the prevention and diagnosis of hyperthermia,insect-borne infectious diseases,tropic skin diseases,infectious diarrhea,and pest harms of disaster victims and rescue team staff should be emphasized during the rescue operations.
文摘Objective：To evaluate the technical characteristics and application of mass casualty incident （MCI） primary triage （PT） methods applied in China.Data Sources：Chinese literature was searched by Chinese Academic Journal Network Publishing Database （founded in June 2014）.The English literature was searched by PubMed （MEDLINE） （1950 to June 2014）.We also searched Official Websites of Chinese Central Government＇s （http：//www.gov.cn/）,National Health and Family Planning Commission of China （http：//www.nhfpc.gov.cn/）,and China Earthquake Information （http：//www.csi.ac.cn/）.Study Selection：We included studies associated with mass casualty events related to China,the PT applied in China,guidelines and standards,and application and development of the carding PT method in China.Results：From 3976 potentially relevant articles,22 met the inclusion criteria,20 Chinese,and 2 English.These articles included 13 case reports,3 retrospective analyses of MCI,two methods introductions,three national or sectoral criteria,and one simulated field testing and validation.There were a total of 19 kinds ofMCI PT methods that have been reported in China from 1950 to 2014.In addition,there were 15 kinds of PT methods reported in the literature from the instance of the application.Conclusions：The national and sectoral current triage criteria are developed mainly for earthquake relief.Classification is not clear.Vague criteria （especially between moderate and severe injuries） operability are not practical.There are no triage methods and research for children and special populations.There is no data and evidence supported triage method.We should revise our existing classification and criteria so it is clearer and easier to be grasped in order to build a real,practical,and efficient PT method.
文摘Background：Inflammation plays a pivotal role in the formation and progression of ischemic stroke.Recently,more and more epidemiological studies have focused on the association between C-reactive protein （CRP）-717A 〉 G and-286C 〉 T 〉 A genetic polymorphisms and ischemic stroke.However,the findings of these researches are not conclusive.Methods：We performed a meta-analysis to determine whether these two polymorphisms are associated with the risk of ischemic stroke.Eligible studies were identified from the database of PubMed,Medline,Embase,Web of Science,CNKI,Weipu,and Wanfang.We calculated odds ratios （ORs） with 95% confidence intervals （CIs） to assess the strength of the association.Results：Four articles were included in our study,including 1926 cases and 2678 controls for-717A 〉 G polymorphism,652 cases and 1103 controls for-286C 〉 T 〉 A polymorphism.The results of meta-analysis showed that single nucleotide polymorphism （SNP）-717A 〉 G was not significantly associated with the risk of ischemic stroke （GG vs.AA,OR =1.12,95% CI =0.83-1.50,P =0.207;GG ＋ GA vs.AA,OR =1.04,95% CI =0.93-1.17,P =0.533;GG vs.GA ＋ AA,OR =1.10,95% CI =0.82-1.47,P =0.220）.Meta-analysis of SNP-286C 〉 T 〉 A also demonstrated no statistical evidence of a significant association with the risk of ischemic stroke （AA vs.CC,OR =0.86,95% CI =0.59-1.25,P =0.348;AA vs.CC,OR =0.92,95% CI =0.80-1.06,P =0.609;AA vs.CC,OR =0.89,95% CI =0.62-1.30,P =0.374）.Conclusions：This meta-analysis demonstrated little evidence to support a role of CRP gene-717A 〉 G,-286C 〉 T 〉 A polymorphisms in ischemic stroke predisposition.However,to draw comprehensive and more reliable conclusions,further larger studies are needed to validate the association between CRP gene polymorphisms and ischemic stroke in various ethnic groups.
